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Biochemistry & Molecular Biology
Hsiang-Pu Feng, Hsiao-Yun Cheng, Ting-Feng Hsiao, Tai-Wei Lin, Jia-Wei Hsu, Lien-Hung Huang, Chia-Jung Yu
Summary: ADP-ribosylation factors (Arfs) and Arf-like (Arl) GTPases are crucial regulators of intracellular vesicle trafficking and Golgi structure. In this study, it was found that ArfGAP1 can promote GTP hydrolysis of Arl1, affecting its function at the trans-Golgi network (TGN).
Article
Biochemistry & Molecular Biology
Niels Boegholm, Narcis A. Petriman, Marta Loureiro-Lopez, Jiaolong Wang, Miren Itxaso Santiago Vela, Beibei Liu, Tomoharu Kanie, Roy Ng, Peter K. Jackson, Jens S. Andersen, Esben Lorentzen
Summary: Cilia are important cellular organelles for signaling and motility and are constructed via intraflagellar transport (IFT). RabL2 localizes to the basal body of cilia via an interaction with CEP19 before downstream association with the IFT machinery. Reconstituted pentameric IFT complex containing IFT81/74 enhances the GTP hydrolysis rate of RabL2. Structural models of RabL2-containing IFT complexes validate the architectural understanding of RabL2 incorporation into the IFT complex.
Article
Endocrinology & Metabolism
Xue Wen Ng, Yong Hee Chung, Farzad Asadi, Chen Kong, Alessandro Ustione, David W. Piston
Summary: Excessive glucagon secretion from pancreatic alpha-cells contributes to hyperglycemia in both type 1 and type 2 diabetes. This study reveals the role of EphA-RhoA signaling pathway in regulating glucagon secretion. The findings provide insights into the mechanisms underlying insulin secretion.
Article
Cell Biology
Lingye Zhang, Anni Zhou, Shengtao Zhu, Li Min, Si Liu, Peng Li, Shutian Zhang
Summary: This review summarizes the biological functions and molecular mechanisms of ARHGAP26 in different tumors, proposes the potential clinical value of ARHGAP26 in cancer treatment, and discusses current issues that need to be addressed.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Xiankui Cui, Shuwei Wang, Yaohui Huang, Xuening Ding, Zirong Wang, Lidan Zheng, Yujing Bi, Fanghui Ge, Lei Zhu, Ming Yuan, Shaul Yalovsky, Ying Fu
Summary: This study uncovers a direct link between an ROP GTPase and vesicular trafficking and a new mechanism for the control of apical exocytosis, whereby ROP GTPase signaling spatially regulates SNARE complex assembly and the polar distribution of a Q-SNARE.
Article
Gastroenterology & Hepatology
Michelle M. Cooley, Diana D. H. Thomas, Kali Deans, Yajing Peng, Aurelia Lugea, Stephen J. Pandol, Luigi Puglielli, Guy E. Groblewski
Summary: The study demonstrated that down-regulation of AT-1 during pancreatitis leads to chronic ER stress and pancreatic inflammation. Loss of AT-1 induces activation of unfolded protein response pathways and causes pancreatic disease characterized by intracellular trypsin accumulation, immune cell infiltration, and fibrosis.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Jianhong An, Tingting Jiang, Ling Qi, Keping Xie
Summary: Pancreatic fibrosis is caused by excessive deposition of collagen and fibronectin extracellular matrix in the pancreatic tissue due to repeated injury in chronic pancreatic diseases. The pathophysiology involves acinar cell injury, duct dysfunction, stellate cell activation, and persistent inflammatory response. The role of acinar cells in pancreatic fibrosis and potential clinical significance are discussed.
CYTOKINE & GROWTH FACTOR REVIEWS
(2023)
Article
Immunology
Rohini Shrivastava, Gourango Pradhan, Sudip Ghosh, Sangita Mukhopadhyay
Summary: This study demonstrates that Rabaptin5 acts as a guanine nucleotide exchange factor for Rab7l1, facilitating its recruitment and activation to regulate phagosome maturation.
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Gastroenterology & Hepatology
Leiling Pan, Medhanie A. Mulaw, Johann Gout, Min Guo, Hina Zarrin, Peggy Schwarz, Bernd Baumann, Thomas Seufferlein, Martin Wagner, Franz Oswald
Summary: This study found that downregulation of RBPJ in PDAC patients promotes KRAS-mediated transformation of pancreatic acinar cells and development of fibrosis. This finding provides important clues for the development mechanism of PDAC and offers a theoretical basis for finding new treatment strategies.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Microbiology
Yu Huang, Shuangquan Yan, Yuzhu Li, Xuefeng Ai, Xi Yu, Yan Ge, Xi Lv, Lin Fan, Jianping Xie
Summary: The study reveals that MfpD, a GTPase-activating protein (GAP), is involved in regulating fluoroquinolone resistance. Through genetic and biochemical experiments, it was shown that MfpD acts as a GAP for MfpB, playing a crucial role in FQs resistance.
MICROBIOLOGY SPECTRUM
(2022)
Article
Cell Biology
Guojie Xu, Lei Zhao, Qingling Hua, Lanqing Wang, Hongli Liu, Zhenyu Lin, Min Jin, Jing Wang, Pengfei Zhou, Kunyu Yang, Gang Wu, Dandan Yu, Dejun Zhang, Tao Zhang
Summary: In this study, it was found that CEMIP interacts with GRAF1, and the combination of high CEMIP and low GRAF1 predicts poor survival of patients. It was also revealed that CEMIP promotes CRC metastasis by negatively regulating the stability of GRAF1 and by bridging MIB1 and GRAF1. Furthermore, it was demonstrated that CEMIP activates the CDC42/MAPK pathway-regulated EMT, which is essential for CEMIP-mediated migration and invasion of CRC cells. It was concluded that CDC42 inhibitor could be a potential therapeutic strategy for CEMIP-mediated CRC metastasis.
CELL DEATH & DISEASE
(2023)
Article
Oncology
Shingo Yoshimachi, Ryutaro Shirakawa, Mingxin Cao, Duc Anh Trinh, Pan Gao, Natsumi Sakata, Kento Miyazaki, Kota Goto, Takayuki Miura, Kyohei Ariake, Shimpei Maeda, Kunihiro Masuda, Masaharu Ishida, Hideo Ohtsuka, Michiaki Unno, Hisanori Horiuchi
Summary: RalA and RalB, small GTPase members of the Ras family, play essential roles in pancreatic ductal adenocarcinoma. Ral proteins are activated by GTP binding and inactivated by GDP binding. Deficiency in RalGAP β leads to increased Ral activity, resulting in enhanced migration, tumor growth, and metastasis in PDAC cells.
Article
Cell Biology
Qi Tian, Huan Gao, Yan Zhou, Lizhe Zhu, Jiao Yang, Bo Wang, Peijun Liu, Jin Yang
Summary: This study elucidates the role and molecular mechanism of RICH1 in regulating stemness of breast cancer cells. RICH1 activates the kinase cascade of the Hippo signaling pathway by displacing Amot-p80 from the complex with Merlin, thereby promoting stemness and inhibiting migration and invasion of breast cancer cells.
CELL DEATH & DISEASE
(2022)
Article
Biotechnology & Applied Microbiology
Wenfei Ji, Lili Zhang, Hongjun Zhu
Summary: This study found that GATA5 and ARHGAP9 are lowly expressed in lung adenocarcinoma and are positively correlated. Further experiments demonstrated that GATA5 can successfully activate ARHGAP9, which significantly inhibits the proliferation, invasion, and migration of lung adenocarcinoma cells. Additionally, the silencing of GATA5 reversed the inhibitory effect of upregulated ARHGAP9 on the malignant progression of lung adenocarcinoma cells.
Article
Biochemistry & Molecular Biology
Eva Wisniewski, Domonkos Czaran, Fanni Kovacs, Eniko Bahurek, Afrodite Nemeth, Peter Sasvari, Gergo Szanda, Aladar Pettko-Szandtner, Eva Klement, Erzsebet Ligeti, Roland Csepanyi-Komi
Summary: This study reveals the regulatory role of different phosphorylation patterns on the GAP activity, superoxide production, and phagocytic effect of ARHGAP25.
Article
Cell Biology
Maria E. Sabbatini, Yan Bi, Baoan Ji, Stephen A. Ernst, John A. Williams
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2010)
Article
Gastroenterology & Hepatology
Stephen J. Crozier, M. Dolors Sans, Jackie Y. Wang, Stephen I. Lentz, Stephen A. Ernst, John A. Williams
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2010)
Article
Gastroenterology & Hepatology
Maria Dolors Sans, Maria Eugenia Sabbatini, Stephen A. Ernst, Louis G. D'Alecy, Ichiko Nishijima, John A. Williams
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2011)
Article
Gastroenterology & Hepatology
Lili Guo, Maria Dolors Sans, Yanan Hou, Stephen A. Ernst, John A. Williams
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2012)
Article
Gastroenterology & Hepatology
John A. Williams
CURRENT OPINION IN GASTROENTEROLOGY
(2010)
Article
Gastroenterology & Hepatology
Grzegorz T. Gurda, Stephen J. Crozier, Baoan Ji, Stephen A. Ernst, Craig D. Logsdon, Beverly A. Rothermel, John A. Williams
Editorial Material
Gastroenterology & Hepatology
John A. Williams
Article
Biochemistry & Molecular Biology
Kamaldeen A. Muili, Dong Wang, Abrahim I. Orabi, Sheharyar Sarwar, Yuhuan Luo, Tanveer A. Javed, John F. Eisses, Syeda M. Mahmood, Shunqian Jin, Vijay P. Singh, Meena Ananthanaravanan, George Perides, John A. Williams, Jeffery D. Molkentin, Sohail Z. Husain
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
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Neurosciences
Maria E. Sabbatini, Louis D'Alecy, Stephen I. Lentz, Tong Tang, John A. Williams
JOURNAL OF PHYSIOLOGY-LONDON
(2013)
Article
Biochemical Research Methods
Xuequn Chen, Maria Dolors Sans, John R. Strahler, Alla Karnovsky, Stephen A. Ernst, George Michailidis, Philip C. Andrews, John A. Williams
JOURNAL OF PROTEOME RESEARCH
(2010)
Review
Gastroenterology & Hepatology
John A. Williams
Article
Cell Biology
Grzegorz T. Gurda, Jackie Y. Wang, Lili Guo, Stephen A. Ernst, John A. Williams
PHYSIOLOGICAL GENOMICS
(2012)
Article
Multidisciplinary Sciences
Maria Eugenia Sabbatini, John A. Williams
Review
Biochemical Research Methods
Xuequn Chen, Alla Karnovsky, Maria Dolors Sans, Philip C. Andrews, John A. Williams