Article
Pharmacology & Pharmacy
Radoslaw Wojcik, Marek R. Baranowski, Lukasz Markiewicz, Dorota Kubacka, Marcelina Bednarczyk, Natalia Baran, Anna Wojtczak, Pawel J. Sikorski, Joanna Zuberek, Joanna Kowalska, Jacek Jemielity
Summary: This passage discusses the utility of dinucleotide analogs of messenger RNA cap, particularly those with benzyl substitution at N7 position, for potential translational inhibition. The synthesis of novel Bn(7)GpppG analogs and their evaluation in terms of translation and translational inhibition are highlighted, showing promising results for N7-(4-halogenbenzyl) substitutions. Additionally, crystal structures of compounds with eIF4E were determined to gain more molecular insight into N7-benzylation of mRNA cap.
Review
Cell Biology
Angelica Montiel-Davalos, Yeniffer Ayala, Greco Hernandez
Summary: Among CNS cancers, glioblastoma (GB) is the most common and aggressive type. Current treatments involve surgery, radiation, and chemotherapy, but the prognosis remains poor. Recent studies have focused on mRNA translation and its dysregulation in GB, particularly in the initiation phase. The translation machinery and ribosomal proteins have also been implicated in GB development. This review discusses the relationship between translation initiation, the translation machinery, and GB, as well as the potential of targeting the translation machinery for improved patient survival.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Manfei Liang, Clara Hody, Vanessa Yammine, Romuald Soin, Yuqiu Sun, Xing Lin, Xiaoying Tian, Romane Meurs, Camille Perdrau, Nadege Delacourt, Marina Oumalis, Fabienne Andris, Louise Conrard, Veronique Kruys, Cyril Gueydan
Summary: Hypoxia causes changes in gene expression in eukaryotic cells by reducing ATP supply and inhibiting protein synthesis. This study shows that a CA-rich motif in the 3'untranslated region of Ldh mRNA allows it to be highly translated in hypoxia. The cap-binding protein eIF4EHP is identified as a key factor in 3'UTR-dependent translation under low oxygen conditions, and it is also necessary for Drosophila development and mobility after hypoxic challenge.
Article
Oncology
Sho Takahashi, Shusaku Shibutani, Hiroyuki Iwata
Summary: This study investigated the relationship between the localization and phosphorylation of 4E-BP1. The results showed efficient phosphorylation of 4E-BP1 at lysosomes and the plasma membrane, while it was decreased in the nucleus. Additionally, nuclear-localized TORCAR altered cell cycle distribution and reduced cyclin D1 protein levels.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Tamari Chkuaseli, K. Andrew White
Summary: Many eukaryotic RNA viruses regulate gene expression through transcription of subgenomic mRNA, which is controlled by intragenomic interactions forming higher-order RNA structures. However, a new study reveals that an umbravirus activates sg mRNA transcription through base pair-mediated dimerization of its RNA genome. The study provides evidence of a kissing-loop interaction that forms a dimer,located just upstream of the transcriptional initiation site, and is involved in transcriptional activation. This unconventional transcription strategy may be utilized by other similar viruses.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Monica Mesa-Perez, Phineas T. Hamilton, Alex Miranda, Nicholas Brodie, Connor O'Sullivan, Jennifer Christie, Bridget C. Ryan, Robert L. Chow, David Goodlett, Christopher J. Nelson, Perry L. Howard
Summary: The lifecycle of RNA polymerase II (RNAPII) transcripts is influenced by the formation of mutually exclusive ribonucleoprotein complexes (RNPs) that guide transcript generation and degradation. A recent study reveals that the scaffold protein ARS2, bound to the cap binding complex (CBC), regulates RNA metabolism in the nucleus. Interestingly, alternative splicing of ARS2 generates cytoplasmic isoforms that exhibit different functions from their nuclear counterparts, suggesting their importance in cellular homeostasis and stress response.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Guofang Hou, Xian Zhao, Lian Li, Qianqian Yang, Xiaojia Liu, Caihu Huang, Runhui Lu, Ran Chen, Yanli Wang, Bin Jiang, Jianxiu Yu
Summary: Our study reveals that YTHDF2 can be SUMOylated, playing a crucial role in cancer progression.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Maria J. R. Bezerra, Danielle M. N. Moura, Eden R. Freire, Fabiola B. Holetz, Christian R. S. Reis, Tallyta T. S. Monteiro, Adriana R. S. Pinto, Ning Zhang, Antonio M. Rezende, Antonio Pereira-Neves, Regina C. B. Q. Figueiredo, Christine Clayton, Mark C. Field, Mark Carrington, Osvaldo P. de Melo Neto
Summary: Translation in pathogenic protozoans of the family Trypanosomatidae involves multiple eIF4F-like complexes, with the EIF4E4/EIF4G3 and EIF4E3/EIF4G4 pairs being the most prominent. These complexes exhibit distinct protein partners and play important roles in gene expression and cell morphology. Knockdown studies revealed that EIF4E3/EIF4G4 are essential for viability and are involved in cytokinesis. The differential association of mRNAs with each complex was also identified through CLIP-seq analysis, further confirming their distinct functions.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Multidisciplinary Sciences
Vladimir Despic, Samie R. R. Jaffrey
Summary: The mRNA cap structure is a major site of dynamic mRNA methylation, with Cap1 and Cap2 forms depending on the presence of 2'-O-methylation on the first transcribed nucleotide or both the first and second transcribed nucleotides, respectively. The identity and function of Cap2-containing mRNAs are currently unclear.
Article
Biochemistry & Molecular Biology
Kirk B. Jensen, B. Kate Dredge, John Toubia, Xin Jin, Valentina Iadevaia, Gregory J. Goodall, Christopher G. Proud
Summary: This study introduces a novel method, capCLIP, to comprehensively capture and quantify the eIF4E 'cap-ome' and investigate the biological consequences of eIF4E-cap binding in different cellular contexts. The results from analyzing human cells with mTORC1 inhibitor rapamycin and examining phosphorylation of eIF4E demonstrate that capCLIP is a valuable tool for studying the function of eIF4E and other cap-binding proteins.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Amarnath Bollu, Helena Schepers, Nils Kloecker, Mehmet Erguven, Ann-Marie Lawrence-Doerner, Andrea Rentmeister
Summary: Protein synthesis is crucial in cells and regulated by various mechanisms. Cytoplasmic cap methylation, the final step of cytoplasmic recapping, can be activated by a photocaged cap. This process plays an important role in cells.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Cell Biology
Vytaute Boreikaite, Thomas S. Elliott, Jason W. Chin, Lori A. Passmore
Summary: In this study, the researchers reconstituted the 3' endonuclease activity of human CPSF and identified the protein factors required for this activity. They found that the activation and site-specific pre-mRNA cleavage by CPSF are tightly controlled. The results suggest that fidelity in mRNA processing is maintained through precise regulation of CPSF activity.
GENES & DEVELOPMENT
(2022)
Article
Oncology
Qianqian Chen, Yiling Wang, Lin Yang, Liyuan Sun, Yuxin Wen, Yongyi Huang, Kaiping Gao, Wenhan Yang, Feng Bai, Lijuan Ling, Zizi Zhou, Xiaoming Zhang, Juan Xiong, Rihong Zhai
Summary: This study revealed that PM2.5 activated the overexpression of DLAT and enhanced glycolysis metabolism, contributing to the tumorigenesis of NSCLC. DLAT-associated pathway may be a potential therapeutic target for NSCLC.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Endocrinology & Metabolism
Jaclyn E. Welles, Allyson L. Toro, Siddharth Sunilkumar, Shaunaci A. Stevens, Carson J. Purnell, Scot R. Kimball, Michael D. Dennis
Summary: Increased expression of the peptide hormone retinol-binding protein 4 (RBP4) has been linked to insulin resistance and type 2 diabetes. Studies have shown that translational control of RBP4 synthesis plays a role in its regulation, with activation of mTORC1 kinase promoting hepatic Rbp4 mRNA translation. Targeting Rbp4 mRNA translation could be an alternative therapeutic approach to reduce serum RBP4 levels.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Chemistry, Multidisciplinary
Mateusz Kozarski, Karolina Drazkowska, Marcelina Bednarczyk, Marcin Warminski, Jacek Jemielity, Joanna Kowalska
Summary: mRNA-based gene delivery is a powerful strategy for vaccines and therapeutics. This study synthesized novel triazole-containing tri- and tetranucleotide cap analogs through copper-catalyzed azide-alkyne cycloaddition. One of the compounds showed translational activity and biochemical properties comparable to natural cap structure, making it a promising mRNA capping reagent.
Article
Oncology
Xiangyun Li, Mei Li, Hang Ruan, Wei Qiu, Xiang Xu, Lin Zhang, Jian Yu
Article
Medicine, Research & Experimental
Yi Yang, Xiao He, Rongseng Zhao, Wei Guo, Ming Zhu, Wei Xing, Dongpo Jiang, Chongyang Liu, Xiang Xu
JOURNAL OF TRANSLATIONAL MEDICINE
(2018)
Article
Medical Laboratory Technology
Xiao He, Shanmu Ai, Wei Guo, Yi Yang, Zhengguo Wang, Dongpo Jiang, Xiang Xu
TRANSLATIONAL RESEARCH
(2018)
Article
Cell & Tissue Engineering
Xiao He, Meng-Wei Yao, Ming Zhu, Dong-Lan Liang, Wei Guo, Yi Yang, Rong-Seng Zhao, Ting-Ting Ren, Xiang Ao, Wei Wang, Chun-Yu Zeng, Hua-Ping Liang, Dong-po Jiang, Jian Yu, Xiang Xu
STEM CELL RESEARCH & THERAPY
(2018)
Review
Oncology
Daqian Gu, Xiang Ao, Yu Yang, Zhuo Chen, Xiang Xu
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2018)
Article
Oncology
Xiang Ao, Yu Yang, Weiqiang Li, Yan Tan, Wei Guo, Luoquan Ao, Xiao He, Xiaofeng Wu, Jianchuan Xia, Xiang Xu, Jianxin Guo
JOURNAL OF IMMUNOTHERAPY
(2019)
Article
Critical Care Medicine
Xuanfei Li, Junyu Zhu, Lixing Tian, Xiaoyuan Ma, Xia Fan, Li Luo, Jing Yu, Yu Sun, Xue Yang, Wanqi Tang, Wei Ma, Jun Yan, Xiang Xu, Huaping Liang
CRITICAL CARE MEDICINE
(2020)
Article
Pharmacology & Pharmacy
Wei Guo, Wei Qiu, Xiang Ao, Weiqiang Li, Xiao He, Luoquan Ao, Xueting Hu, Zhan Li, Ming Zhu, Donglin Luo, Wei Xing, Xiang Xu
BRITISH JOURNAL OF PHARMACOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Zhan Li, Yuqing Song, Peisong Yuan, Wei Guo, Xueting Hu, Wei Xing, Luoquan Ao, Yan Tan, Xiaofeng Wu, Xiang Ao, Xiao He, Dongpo Jiang, Huaping Liang, Xiang Xu
Article
Ophthalmology
J. Li, Q. S. Ran, B. Hao, X. Xu, H. F. Yuan
JOURNAL OF OPHTHALMOLOGY
(2020)
Review
Cell Biology
Meng Kou, Li Huang, Jinjuan Yang, Zhixin Chiang, Shaoxiang Chen, Jie Liu, Liyan Guo, Xiaoxian Zhang, Xiaoya Zhou, Xiang Xu, Xiaomei Yan, Yan Wang, Jinqiu Zhang, Aimin Xu, Hung-fat Tse, Qizhou Lian
Summary: Mesenchymal stem cells (MSCs) have paracrine effects in therapy, and extracellular vesicles (EVs) play a crucial role in intercellular communication. MSC-EVs have potential therapeutic applications and play an important role in fibrosis treatment and tissue repair.
CELL DEATH & DISEASE
(2022)
Article
Oncology
Xiaohui Wang, Wei Qiu, Haoyu Liu, Min He, Wei He, Zhan Li, Zhiqiang Wu, Xiang Xu, Ping Chen
Summary: This study describes a novel technique for optimizing CAR-T/NK cells for the treatment of antigenically-diverse solid tumors. By arming CAR133-NK92 cells with a CBLB502 TLR5 agonist, specific elimination of CD133-positive colon cancer cells and indirect eradication of CD133-negative colon cancer cells were demonstrated.
CANCER BIOLOGY & MEDICINE
(2023)
Article
Cell & Tissue Engineering
Zhuo Chen, Meng-Wei Yao, Zhi-Lin Shen, Shi-Dan Li, Wei Xing, Wei Guo, Zhan Li, Xiao-Feng Wu, Luo-Quan Ao, Wen-Yong Lu, Qi-Zhou Lian, Xiang Xu, Xiang Ao
Summary: The synergistic effect of IFN-γ and TNF-α can enhance the expression of PD-L1 in hUC-MSCs, thereby increasing their immunosuppressive capacity and suggesting their potential application in ulcerative colitis.
WORLD JOURNAL OF STEM CELLS
(2023)
Article
Pharmacology & Pharmacy
Fang-qiang Zhu, Min-jia Chen, Ming Zhu, Rong-seng Zhao, Wei Qiu, Xiang Xu, Hong Liu, Hong-wen Zhao, Rong-jie Yu, Xiong-fei Wu, Keqin Zhang, Hong Huang
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2017)
Article
Endocrinology & Metabolism
Ming Zhu, Xiao He, Xiao-Hui Wang, Wei Qiu, Wei Xing, Wei Guo, Tian-Chen An, Luo-Quan Ao, Xue-Ting Hu, Zhan Li, Xiao-Ping Liu, Nan Xiao, Jian Yu, Hong Huang, Xiang Xu
