Microfluidics: reframing biological enquiry

Food for thought

(2015)   Nature Chemical Biology

Single-Cell Western Blotting after Whole-Cell Imaging to Assess Cancer Chemotherapeutic Response

(2014) Chi-Chih Kang et al.   ANALYTICAL CHEMISTRY

Microfluidics-Based Single-Cell Functional Proteomics for Fundamental and Applied Biomedical Applications

(2014) Jing Yu et al.   Annual Review of Analytical Chemistry

A microfluidic 3D in vitro model for specificity of breast cancer metastasis to bone

(2014) Simone Bersini et al.   BIOMATERIALS

Integrated chip-based physiometer for automated fish embryo toxicity biotests in pharmaceutical screening and ecotoxicology

(2014) Jin Akagi et al.   CYTOMETRY PART A

Using physiologically-based pharmacokinetic-guided “body-on-a-chip” systems to predict mammalian response to drug and chemical exposure

(2014) Jong Hwan Sung et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Microfluidic analysis of red blood cell deformability

(2014) Quan Guo et al.   JOURNAL OF BIOMECHANICS

Just the FACS

(2014) Lewis L. Lanier   JOURNAL OF IMMUNOLOGY

Human NK Cells Licensed by Killer Ig Receptor Genes Have an Altered Cytokine Program That Modifies CD4+T Cell Function

(2014) Lin Lin et al.   JOURNAL OF IMMUNOLOGY

Microfluidic organs-on-chips

(2014) Sangeeta N Bhatia et al.   NATURE BIOTECHNOLOGY

Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption

(2014) Benjamin L Wang et al.   NATURE BIOTECHNOLOGY

Universal process-inert encoding architecture for polymer microparticles

(2014) Jiseok Lee et al.   NATURE MATERIALS

Modeling the mitochondrial cardiomyopathy of Barth syndrome with induced pluripotent stem cell and heart-on-chip technologies

(2014) Gang Wang et al.   NATURE MEDICINE

Single-cell western blotting

(2014) Alex J Hughes et al.   NATURE METHODS

Glioblastoma cellular architectures are predicted through the characterization of two-cell interactions

(2014) N. Kravchenko-Balasha et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Microfluidic single-cell whole-transcriptome sequencing

(2014) A. M. Streets et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Programmable on-chip DNA compartments as artificial cells

(2014) E. Karzbrun et al.   SCIENCE

Optimization of 3-D organotypic primary colonic cultures for organ-on-chip applications

(2014) Asad A Ahmad et al.   Journal of Biological Engineering

System wide analyses have underestimated protein abundances and the importance of transcription in mammals

(2014) Jingyi Jessica Li et al.   PeerJ

Development of a Capillary Electrophoresis Platform for Identifying Inhibitors of Protein–Protein Interactions

(2013) Jennifer N. Rauch et al.   ANALYTICAL CHEMISTRY

The Potential Impact of Droplet Microfluidics in Biology

(2013) Thomas Schneider et al.   ANALYTICAL CHEMISTRY

High-Throughput Microfluidic Single-Cell Digital Polymerase Chain Reaction

(2013) A. K. White et al.   ANALYTICAL CHEMISTRY

Modeling of cancer metastasis and drug resistance via biomimetic nano-cilia and microfluidics

(2013) Ching-Te Kuo et al.   BIOMATERIALS

Fish in chips: an automated microfluidic device to study drug dynamics in vivo using zebrafish embryos

(2013) Chunhong Zheng et al.   CHEMICAL COMMUNICATIONS

Exploring Living Multicellular Organisms, Organs, and Tissues Using Microfluidic Systems

(2013) Venkataragavalu Sivagnanam et al.   CHEMICAL REVIEWS

Microfluidic interrogation and mathematical modeling of multi-regime calcium signaling dynamics

(2013) Andreja Jovic et al.   Integrative Biology

Complex chemoattractive and chemorepellent Kit signals revealed by direct imaging of murine mast cells in microfluidic gradient chambers

(2013) Amir Shamloo et al.   Integrative Biology

Rapid Discovery of a Novel Series of Abl Kinase Inhibitors by Application of an Integrated Microfluidic Synthesis and Screening Platform

(2013) Bimbisar Desai et al.   JOURNAL OF MEDICINAL CHEMISTRY

Ultrafast Hydrogen Exchange Reveals Specific Structural Events during the Initial Stages of Folding of Cytochrome c

(2013) Hossein Fazelinia et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Pinched-flow hydrodynamic stretching of single-cells

(2013) Jaideep S. Dudani et al.   LAB ON A CHIP

Ultrafast cell switching for recording cell surface transitions: new insights into epidermal growth factor receptor signalling

(2013) Ya-Yu Chiang et al.   LAB ON A CHIP

Liver-cell patterning Lab Chip: mimicking the morphology of liver lobule tissue

(2013) Chen-Ta Ho et al.   LAB ON A CHIP

Microfabricated mammalian organ systems and their integration into models of whole animals and humans

(2013) Jong H. Sung et al.   LAB ON A CHIP

A yeast one-hybrid and microfluidics-based pipeline to map mammalian gene regulatory networks

(2013) Carine Gubelmann et al.   Molecular Systems Biology

Quantitative assessment of single-cell RNA-sequencing methods

(2013) Angela R Wu et al.   NATURE METHODS

The promise of single-cell sequencing

(2013) James Eberwine et al.   NATURE METHODS

Construction and use of a microfluidic dissection platform for long-term imaging of cellular processes in budding yeast

(2013) Daphne H E W Huberts et al.   Nature Protocols

Single molecule quantitation and sequencing of rare translocations using microfluidic nested digital PCR

(2013) Joe Shuga et al.   NUCLEIC ACIDS RESEARCH

Microfluidic processor allows rapid HER2 immunohistochemistry of breast carcinomas and significantly reduces ambiguous (2+) read-outs

(2013) A. T. Ciftlik et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Biased migration of confined neutrophil-like cells in asymmetric hydraulic environments

(2013) H. V. Prentice-Mott et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Biomimetic model to reconstitute angiogenic sprouting morphogenesis in vitro

(2013) D.-H. T. Nguyen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Quantitative Diagnosis of Malignant Pleural Effusions by Single-Cell Mechanophenotyping

(2013) H. T. K. Tse et al.   Science Translational Medicine

Molecular phenotyping of aging in single yeast cells using a novel microfluidic device

(2012) Zhengwei Xie et al.   AGING CELL

Microfluidics-Based Assessment of Cell Deformability

(2012) Andrea Adamo et al.   ANALYTICAL CHEMISTRY

Amplification and Temporal Filtering during Gradient Sensing by Nerve Growth Cones Probed with a Microfluidic Assay

(2012) Mathieu Morel et al.   BIOPHYSICAL JOURNAL

Genome-wide Single-Cell Analysis of Recombination Activity and De Novo Mutation Rates in Human Sperm

(2012) Jianbin Wang et al.   CELL

Bistable Isoelectric Point Photoswitching in Green Fluorescent Proteins Observed by Dynamic Immunoprobed Isoelectric Focusing

(2012) Alex J. Hughes et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Engineers are from PDMS-land, Biologists are from Polystyrenia

(2012) Erwin Berthier et al.   LAB ON A CHIP

Programmable microfluidic synthesis of spectrally encoded microspheres

(2012) R. E. Gerver et al.   LAB ON A CHIP

Droplet microfluidics for high-throughput biological assays

(2012) Mira T. Guo et al.   LAB ON A CHIP

Characterization of a microfluidic in vitro model of the blood-brain barrier (μBBB)

(2012) Ross Booth et al.   LAB ON A CHIP

A microfluidic device and computational platform for high-throughput live imaging of gene expression

(2012) Wolfgang Busch et al.   NATURE METHODS

Systematic reconstruction of RNA functional motifs with high-throughput microfluidics

(2012) Lance Martin et al.   NATURE METHODS

Highly multiplexed and strand-specific single-cell RNA 5′ end sequencing

(2012) Saiful Islam et al.   Nature Protocols

Erratum: Regulation of cytoplasmic mRNA decay

(2012) Daniel R. Schoenberg et al.   NATURE REVIEWS GENETICS

Microfluidics separation reveals the stem-cell-like deformability of tumor-initiating cells

(2012) W. Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Innate immune response to homologous rotavirus infection in the small intestinal villous epithelium at single-cell resolution

(2012) A. Sen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Whole lifespan microscopic observation of budding yeast aging through a microfluidic dissection platform

(2012) S. S. Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Migration of cells in a social context

(2012) S. Vedel et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Serine phosphorylation by SYK is critical for nuclear localization and transcription factor function of Ikaros

(2012) F. M. Uckun et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Basic leucine zipper transcription factor Hac1 binds DNA in two distinct modes as revealed by microfluidic analyses

(2012) P. M. Fordyce et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In vitro microvessels for the study of angiogenesis and thrombosis

(2012) Y. Zheng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Hydrodynamic stretching of single cells for large population mechanical phenotyping

(2012) D. R. Gossett et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Massively parallel measurements of molecular interaction kinetics on a microfluidic platform

(2012) M. Geertz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Functional single-cell hybridoma screening using droplet-based microfluidics

(2012) B. E. Debs et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Three-dimensional microfluidic model for tumor cell intravasation and endothelial barrier function

(2012) I. K. Zervantonakis et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mitochondrial Network Size Scaling in Budding Yeast

(2012) S. M. Rafelski et al.   SCIENCE

Genome-Wide Detection of Single-Nucleotide and Copy-Number Variations of a Single Human Cell

(2012) C. Zong et al.   SCIENCE

Microfluidic Investigation of BDNF-Enhanced Neural Stem Cell Chemotaxis in CXCL12 Gradients

(2012) Hui Xu et al.   Small

CEL-Seq: Single-Cell RNA-Seq by Multiplexed Linear Amplification

(2012) Tamar Hashimshony et al.   Cell Reports

Identification and Targeting of an Interaction between a Tyrosine Motif within Hepatitis C Virus Core Protein and AP2M1 Essential for Viral Assembly

(2012) Gregory Neveu et al.   PLoS Pathogens

The Role of Body-on-a-Chip Devices in Drug and Toxicity Studies

(2011) M.B. Esch et al.   Annual Review of Biomedical Engineering

Shear Stress Regulates Adhesion and Rolling of CD44+ Leukemic and Hematopoietic Progenitor Cells on Hyaluronan

(2011) Christof Christophis et al.   BIOPHYSICAL JOURNAL

Protein Signaling Networks from Single Cell Fluctuations and Information Theory Profiling

(2011) Young Shik Shin et al.   BIOPHYSICAL JOURNAL

In vivo measurement of signaling cascade dynamics

(2011) Megan N. McClean et al.   CELL CYCLE

In vitro modeling of the microvascular occlusion and thrombosis that occur in hematologic diseases using microfluidic technology

(2011) Michelle Tsai et al.   JOURNAL OF CLINICAL INVESTIGATION

Fish and Chips: a microfluidic perfusion platform for monitoring zebrafish development

(2011) Deepak Choudhury et al.   LAB ON A CHIP

A clinical microchip for evaluation of single immune cells reveals high functional heterogeneity in phenotypically similar T cells

(2011) Chao Ma et al.   NATURE MEDICINE

Automated protein-DNA interaction screening of Drosophila regulatory elements

(2011) Korneel Hens et al.   NATURE METHODS

Stiffness Gradients Mimicking In Vivo Tissue Variation Regulate Mesenchymal Stem Cell Fate

(2011) Justin R. Tse et al.   PLoS One

Observation of spatial propagation of amyloid assembly from single nuclei

(2011) T. P. J. Knowles et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-resolution dose-response screening using droplet-based microfluidics

(2011) O. J. Miller et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dendritic cell chemotaxis in 3D under defined chemokine gradients reveals differential response to ligands CCL21 and CCL19

(2011) U. Haessler et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-throughput microfluidic single-cell RT-qPCR

(2011) A. K. White et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single-cell proteomic chip for profiling intracellular signaling pathways in single tumor cells

(2011) Q. Shi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Onset of Turbulence in Pipe Flow

(2011) K. Avila et al.   SCIENCE

Microfluidic Cardiac Cell Culture Model (μCCCM)

(2010) Guruprasad A. Giridharan et al.   ANALYTICAL CHEMISTRY

Quantitative proteomic profiling of breast cancers using a multiplexed microfluidic platform for immunohistochemistry and immunocytochemistry

(2010) Minseok S. Kim et al.   BIOMATERIALS

Thrombin Flux and Wall Shear Rate Regulate Fibrin Fiber Deposition State during Polymerization under Flow

(2010) K.B. Neeves et al.   BIOPHYSICAL JOURNAL

Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine

(2010) Tania Konry et al.   BIOSENSORS & BIOELECTRONICS

Rolling on E- or P-selectin induces the extended but not high-affinity conformation of LFA-1 in neutrophils

(2010) Y. Kuwano et al.   BLOOD

A Microfluidic Platform for Systems Pathology: Multiparameter Single-Cell Signaling Measurements of Clinical Brain Tumor Specimens

(2010) J. Sun et al.   CANCER RESEARCH

High-Throughput Screening of Enzymes by Retroviral Display Using Droplet-Based Microfluidics

(2010) Lucia Granieri et al.   CHEMISTRY & BIOLOGY

Robust Growth of Escherichia coli

(2010) Ping Wang et al.   CURRENT BIOLOGY

Damped oscillations in the adaptive response of the iron homeostasis network ofE. coli

(2010) Amnon Amir et al.   MOLECULAR MICROBIOLOGY

Cell mechanics and the cytoskeleton

(2010) Daniel A. Fletcher et al.   NATURE

De novo identification and biophysical characterization of transcription-factor binding sites with microfluidic affinity analysis

(2010) Polly M Fordyce et al.   NATURE BIOTECHNOLOGY

Measurement of Contractile Stress Generated by Cultured Rat Muscle on Silicon Cantilevers for Toxin Detection and Muscle Performance Enhancement

(2010) Kerry Wilson et al.   PLoS One

External and internal constraints on eukaryotic chemotaxis

(2010) D. Fuller et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

(2010) J. J. Agresti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reconstituting Organ-Level Lung Functions on a Chip

(2010) D. Huh et al.   SCIENCE

Microfluidic system for formation of PC-3 prostate cancer co-culture spheroids

(2009) Amy Y. Hsiao et al.   BIOMATERIALS

Toward an Artificial Golgi: Redesigning the Biological Activities of Heparan Sulfate on a Digital Microfluidic Chip

(2009) Jeffrey G. Martin et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Inertial microfluidics

(2009) Dino Di Carlo   LAB ON A CHIP

Material properties of the cell dictate stress-induced spreading and differentiation in embryonic stem cells

(2009) Farhan Chowdhury et al.   NATURE MATERIALS

Tracking lineages of single cells in lines using a microfluidic device

(2009) A. C. Rowat et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cell Growth and Size Homeostasis in Proliferating Animal Cells

(2009) A. Tzur et al.   SCIENCE

Analyzing cell mechanics in hematologic diseases with microfluidic biophysical flow cytometry

(2008) Michael J. Rosenbluth et al.   LAB ON A CHIP