Article
Cell Biology
Takafumi Ogawa, Koji Masumura, Yuki Kohara, Muneyoshi Kanai, Tomoyoshi Soga, Yoshikazu Ohya, T. Keith Blackwell, Masaki Mizunuma
Summary: Methionine restriction can extend lifespan and delay aging-associated pathologies. Supplementation with S-adenosyl-L-homocysteine (SAH) can mimic the effects of methionine restriction by reducing methionine levels and activating AMP-activated protein kinase (AMPK), thereby extending lifespan.
Article
Biochemistry & Molecular Biology
Mangal S. Kadam, V. L. S. Prasad Burra
Summary: Methylation plays a significant role in various diseases, and methyltransferases are important targets for drug development. However, the function of methyltransferases relies on the presence of SAM. This article provides a comprehensive analysis of SAM-receptor interactions and reveals crucial insights into the structure and function of SAM.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Ryuichi Iwasaki, Tomohiro Bito, Atsushi Ishihara, Fumio Watanabe, Yukinori Yabuta
Summary: SAM and SAH are important biochemical intermediates, with SAM being the major methyl donor in vivo. The SAM to SAH ratio is a marker of methylation capacity. Stable isotope-labeled SAM and SAH are used to measure this ratio with high sensitivity. We focused on the SAHH of Pyrococcus horikoshii OT3 and prepared recombinant P. horikoshii SAHH to efficiently produce labeled SAH.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Zhong-Ce Hu, Chui-Mu Zheng, Yun-Chao Tao, Shu-Nan Wang, Yuan-Shan Wang, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: This study aimed to increase the accumulation of S-adenosyl-L-methionine (SAM) in Saccharomyces cerevisiae by promoting ATP availability. The results showed that the lack of gene sod1 benefited ATP generation and positively regulated SAM synthesis. Additionally, improving substrate assimilation further enhanced SAM production. The SAM concentration of strain Delta SOD1 was increased from 7.3 g L-1 to 10.1 g L-1 in a 5-L fermenter.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Chang Dong, J. Carl Schultz, Wei Liu, Jiazhang Lian, Lei Huang, Zhinan Xu, Huimin Zhao
Summary: This study identified novel targets for enhancing SAM production through genome-scale engineering (GSE) and validated their effects in industrial yeast strains, leading to significant increases in SAM productivity. Transcriptomic and metabolomic analyses were conducted to investigate the molecular mechanisms for enhanced SAM accumulation.
METABOLIC ENGINEERING
(2021)
Article
Cell Biology
Renata Orlowska, Jacek Zebrowski, Janusz Zimny, Piotr Androsiuk, Piotr Tomasz Bednarek
Summary: This study used a structural equation modeling approach to analyze the effects of Cu(II) and Ag(I) ions, DNA methylation, and S-adenosyl-L-methionine on the regeneration efficiency of triticale through in vitro culture. The results revealed the relationships between these factors and provided a theoretical model for the biological phenomena associated with green plant regeneration efficiency.
Article
Biotechnology & Applied Microbiology
Siyu Ren, Xinkuan Cheng, Long Ma
Summary: Natural fluorinated products are rare and of great interest. The biosynthetic pathway of de novo fluorometabolites in microbes has been studied, revealing the key roles of fluorinase (FLA) and a novel methionine adenosyltransferase from Streptomyces xinghaiensis (SxMAT) in the synthesis of 5'-fluoro-deoxy-adenosine.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Hailong Chen, Xiaoqin Chai, Yan Wang, Jing Liu, Guohai Zhou, Pinghe Wei, Yuhe Song, Lingman Ma
Summary: Background: Saccharomyces cerevisiae is commonly used for the production of S-adenosyl-l-methionine (SAM), but glucose repression negatively affects its production. This study shows that deleting REG1 and overexpressing SNF1 can improve SAM production in S. cerevisiae by enhancing glucose utilization and glycolysis, promoting ethanol conversion, enhancing precursor amino acid biosynthesis, and extending yeast lifespan.
MICROBIAL CELL FACTORIES
(2022)
Article
Microbiology
Rebeca Dominguez-Santos, Katarina Kosalkova, Isabel-Clara Sanchez-Orejas, Carlos Barreiro, Yolanda Perez-Pertejo, Rosa M. Reguera, Rafael Balana-Fouce, Carlos Garcia-Estrada
Summary: This article describes the use of the filamentous fungus Penicillium chrysogenum in the industrial production of penicillin and highlights the role of AdoMet synthetase in both primary and secondary metabolism in this process.
Review
Oncology
Jiamin Guo, Yanzhong Yang, Ralf Buettner, Steven T. Rosen
Summary: This review summarizes the biological roles of methionine, methionine adenosyl transferase 2A (MAT2A), and S-adenosyl methionine (SAM) in methylation reactions during tumorigenesis. It discusses newly emerged inhibitors targeting the methionine-MAT2A-SAM axis. Recent studies have confirmed the essential roles of methionine and MAT2A in SAM biosynthesis and validated MAT2A as a metabolic dependency of cancer cells. Inhibition of MAT2A has shown synthetic lethality in MTAP-deleted cancers. Notably, significant progress has been made in developing inhibitors targeting the methionine-MAT2A-SAM axis, with first-in-class MAT2A inhibitors entering clinical trials. The methionine-MAT2A-SAM axis plays a critical role in tumorigenesis through providing SAM for abnormal protein, DNA, and RNA methylation in cancer cells. Targeting SAM biosynthesis through MAT2A inhibition has emerged as a novel and promising strategy for cancer therapy.
CURRENT OPINION IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Antonio Francioso, Sergio Fanelli, Maria D'Erme, Eugenio Lendaro, Niccolo Miraglia, Mario Fontana, Rosaria A. Cavallaro, Luciana Mosca
Summary: This study evaluated the pharmacokinetic properties of a new form of SAM, the phytate salt, in rats and found that the phytate anion can protect SAM from degradation, leading to improved pharmacokinetic parameters compared to the SAM tosylated form. These results suggest the potential use of new salts of SAM with better pharmacokinetic properties.
Review
Biotechnology & Applied Microbiology
Jiaojiao Li, Chunxiao Sun, Wenwen Cai, Jing Li, Barry P. Rosen, Jian Chen
Summary: This review systematically discusses eight types of SAM-dependent methyltransferases associated with human diseases, covering their physiological function, substrate specificity, genetic variants, and correlations with disease susceptibility.
MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH
(2021)
Review
Nutrition & Dietetics
Hammad Ullah, Ayesha Khan, Kannan R. R. Rengasamy, Alessandro Di Minno, Roberto Sacchi, Maria Daglia
Summary: Depression is a prevalent and serious health issue globally, and current treatment options are limited. SAMe and probiotics have shown potential therapeutic roles in depression by regulating microbial imbalance and reducing inflammation.
Article
Biochemistry & Molecular Biology
Amanda J. Sales, Izaque S. Maciel, Carlos C. Crestani, Francisco S. Guimara, Samia R. L. Joca
Summary: SAMe exerts antidepressant effects by regulating serotonin synthesis and 5-HT1A receptor activation, reducing immobility time in the forced swimming test in mice.
NEUROCHEMISTRY INTERNATIONAL
(2023)
Article
Biotechnology & Applied Microbiology
Hailong Chen, Nianqing Zhu, Yan Wang, Xinxin Gao, Yuhe Song, Jia Zheng, Jiaping Peng, Xin Zhang
Summary: This study successfully increased the production of SAM in yeast by enhancing glycolysis through deletion of specific genes kcs1 and arg82. The strategy proved to be effective in improving SAM yield, demonstrating the potential for enhancing production of specific metabolites through metabolic engineering.
Article
Biochemistry & Molecular Biology
Pei-Wen Wang, Tung-Yi Lin, Yu-Chiang Hung, Wen-Neng Chang, Pei-Ming Yang, Mu-Hong Chen, Chau-Ting Yeh, Tai-Long Pan
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Genetics & Heredity
Yao-Yu Hsieh, Tsang-Pai Liu, Chia-Jung Chou, Hsin-Yi Chen, Kuen-Haur Lee, Pei-Ming Yang
Article
Chemistry, Multidisciplinary
Guan-Wen Chen, Tai-Yuan Chen, Pei-Ming Yang
Summary: Herbal tea consumption has become popular due to its potential health benefits and attractive flavors. However, there is concern about the contribution of herbal supplements to drug-drug and drug-herb interactions, as well as hepatotoxicity. This study found that herbal tea extracts have differential effects on different modes of hepatotoxicity.
DRUG AND CHEMICAL TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Pei-Ming Yang, Li-Shan Lin, Tsang-Pai Liu
Article
Biochemistry & Molecular Biology
Pei-Ming Yang, Yao-Yu Hsieh, Jia-Ling Du, Shih-Chieh Yen, Chien-Fu Hung
Article
Anatomy & Morphology
Albert Li, Pei-Ming Yang
Article
Biochemistry & Molecular Biology
Tung-Yi Lin, Pei-Wen Wang, Chun-Hsun Huang, Pei-Ming Yang, Tai-Long Pan
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Health Care Sciences & Services
Li-Wei Liu, Yao-Yu Hsieh, Pei-Ming Yang
JOURNAL OF PERSONALIZED MEDICINE
(2020)
Article
Medicine, General & Internal
Jiunn-Chang Lin, Tsang-Pai Liu, Pei-Ming Yang
JOURNAL OF CLINICAL MEDICINE
(2020)
Article
Health Care Sciences & Services
Szu-Jen Wang, Pei-Ming Yang
Summary: This study identified STMN1 as a prognosis biomarker and therapeutic target for HCC, and demonstrated that MST-312 has significant anticancer activity against HCC cells with high STMN1 expression.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Jiunn-Chang Lin, Pei-Ming Yang, Tsang-Pai Liu
Summary: The study identified lncRNA ZFAS1 as a potential biomarker for sorafenib therapy resistance in HCC, and showed that sorafenib induces ER stress and the unfolded protein response pathways through the PERK/ATF4/ZFAS1 signaling axis. This finding suggests a new direction for the treatment and prognosis of HCC patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Health Care Sciences & Services
Jiunn-Chang Lin, Tsang-Pai Liu, Vivin Andriani, Muhammad Athoillah, Chih-Yang Wang, Pei-Ming Yang
Summary: This study identified a gene signature from the transcriptome of HCC patients that correlates with poorer prognoses, which is related to mitotic cell cycle regulation. Gene-drug association analysis suggests that taxanes, such as paclitaxel, may be potential drugs targeting this gene signature. HCC cell lines with mutant TP53 or wild-type CTNNB1 genes show increased sensitivity to paclitaxel treatment, indicating potential benefits for these patient populations.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Pei-Wen Wang, Tung-Yi Lin, Pei-Ming Yang, Chau-Ting Yeh, Tai-Long Pan
Summary: Hepatocellular carcinoma (HCC) development is closely associated with interactions between hepatic stellate cells (HSCs) and various hepatic cells. HSCs play a critical role in modulating extracellular remodeling, cell invasion, and macrophage polarization, thereby enhancing HCC development. This study identifies that the tumor microenvironment created by activated HSCs triggers epithelial-mesenchymal transition (EMT) and affects the tumor-associated immune microenvironment, driving hepatoma cells towards a more malignant phenotype. These findings provide new insights into the role of HSCs in hepatocarcinogenesis and suggest HSCs as a potential therapeutic target for advanced HCC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Li-Hsin Cheng, Chung-Chi Hsu, Hung-Wen Tsai, Wen-Ying Liao, Pei-Ming Yang, Tai-Yan Liao, Hsiao-Yen Hsieh, Tze-Sian Chan, Kelvin K. Tsai
Summary: Small cell lung cancer (SCLC) is a highly aggressive and deadly tumor. The upregulation of neurogenesis-associated protein ASPM-I1 is found in SCLC cells and is associated with poor patient prognosis. ASPM-I1 plays a crucial role in maintaining SCLC stemness and tumorigenesis through the regulation of multiple pathways and the Wnt signaling pathway.
Article
Oncology
Yao-Yu Hsieh, Jia-Ling Du, Pei-Ming Yang
Summary: By employing a gene expression-based drug repositioning strategy, this study identifies VU-0365114 as a novel type of tubulin inhibitor with broad-spectrum anticancer activity, especially in colorectal cancer cells. It is not limited by multidrug resistance proteins and does not exhibit significant off-target effects.
MOLECULAR ONCOLOGY
(2023)