Article
Biochemistry & Molecular Biology
Bartlomiej Stanczykiewicz, Tomasz M. Goszczynski, Pawel Migdal, Marta Piksa, Krzysztof Pawlik, Jakub Gburek, Krzysztof Golab, Boguslawa Konopska, Agnieszka Zablocka
Summary: The research aims to determine the inhibitory activity of ovocystatin, a cysteine protease inhibitor isolated from egg white, on A beta 42 fibril formation in vitro. The results show that ovocystatin possesses A beta 42 anti-aggregation activity and inhibits A beta 42 oligomer toxicity in PC12 cells. This research may contribute to the development of potential substances to prevent or delay beta-amyloid aggregation, a major cause of Alzheimer's disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Tripti Halder, Bharat Patel, Niyati Acharya
Summary: This study developed nanostructured lipid carriers loaded with Asiatic acid (AA) for targeted delivery to the brain and improvement of cognitive deficits in Alzheimer's disease (AD) rats. The optimized carriers showed desired particle size, zeta potential, and entrapment efficiency for brain targeting delivery. In vitro experiments demonstrated better neuroprotective potential of the carriers compared to AA suspension. Pharmacokinetic studies showed increased AA content in plasma and brain after administration of the carriers. The treatment with the carriers significantly improved cognitive deficits in AD rats.
PHARMACEUTICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Alice Filippini, Valentina Salvi, Vincenzo Dattilo, Chiara Magri, Stefania Castrezzati, Robert Veerhuis, Daniela Bosisio, Massimo Gennarelli, Isabella Russo
Summary: The accumulation of amyloid-beta in Alzheimer's disease (AD) brains causes reactive astrogliosis and neuroinflammatory response. This study investigates the role of leucine-rich repeat kinase 2 (LRRK2) in regulating astrocytic activation in response to amyloid-beta(1-42) (A beta(1-42)). The results demonstrate that LRRK2 kinase activity modulates astrocytic reactivity and functions in the presence of A beta(1-42) deposits, suggesting a potential contribution of PD-linked LRRK2 to AD-related neuroinflammation and pathogenesis.
Article
Biochemistry & Molecular Biology
Sareer Ahmad, Myeung Hoon Jo, Muhammad Ikram, Amjad Khan, Myeong Ok Kim
Summary: The study revealed that Luteolin has protective effects against amyloid-beta-induced neuroinflammation, amyloidogenesis, and synaptic dysfunction in mice, potentially through inhibition of JNK signaling and modulation of inflammatory and cell death markers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Renuka Ranjan, Arvind M. Kayastha, Neeraj Sinha
Summary: Researchers conducted preliminary analyses of interactions between amyloid-beta and hydroxyapatite-cholesterol composites, finding that amyloid-beta aggregates only under certain cholesterol concentration conditions in the composites. Solid-state NMR and transmission electron microscopy revealed that the aggregation kinetics of amyloid-beta depend to a certain extent on the exposure and availability of cholesterol surface in the composites.
Article
Clinical Neurology
Lei Liu, Bianca M. Lauro, Amy He, Hyo Lee, Sanjay Bhattarai, Michael S. Wolfe, David A. Bennett, Celeste M. Karch, Tracy Young-Pearse, Dennis J. Selkoe
Summary: This study aims to identify biomarkers for Alzheimer's disease (AD) and finds that the A beta 37/42 ratio can be used to distinguish AD from normal aging. Experimental results show that this ratio outperforms the traditional ratio in differentiating physiological and pathological states in cell culture, brain tissue, and cerebrospinal fluid. The findings may provide a new indicator for early diagnosis of AD.
ALZHEIMERS & DEMENTIA
(2023)
Article
Medicine, Research & Experimental
Julia Ring, Jelena Tadic, Selena Ristic, Michael Poglitsch, Martina Bergmann, Nemanja Radic, Dirk Mossmann, Yongtian Liang, Marta Maglione, Andrea Jerkovic, Roozbeh Hajiraissi, Marcel Hanke, Victoria Kuettner, Heimo Wolinski, Andreas Zimmermann, Lana Domuz Trifunovic, Leonie Mikolasch, Daiana N. Moretti, Filomena Broeskamp, Julia Westermayer, Claudia Abraham, Simon Schauer, Christopher Dammbrueck, Sebastian J. Hofer, Mahmoud Abdellatif, Guido Grundmeier, Guido Kroemer, Ralf J. Braun, Niklas Hansen, Cornelia Sommer, Mirjana Ninkovic, Sandra Seba, Patrick Rockenfeller, Friederike-Nora Voegtle, Joern Dengjel, Chris Meisinger, Adrian Keller, Stephan J. Sigrist, Tobias Eisenberg, Frank Madeo
Summary: In this study, the researchers used yeast and fruit fly models to investigate the role of HSP40 family member Ydj1 in Alzheimer's disease. They found that Ydj1 physically interacts with Abeta42, stabilizes Abeta42 oligomers, and facilitates their translocation to mitochondria, resulting in cell death and memory impairment.
EMBO MOLECULAR MEDICINE
(2022)
Article
Chemistry, Analytical
Houriyeh Sohrabnavi, Mohammad Mohammadimasoudi, Hassan Hajghassem
Summary: This article reports a liquid crystal-based biosensor for the detection of the main biomarker of Alzheimer's disease, amyloid beta protein. The sensor has a fast response time, high specificity, and a low detection limit.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Nanoscience & Nanotechnology
Ana R. Araujo, Juan Correa, Vicente Dominguez-Arca, Rui L. Reis, Eduardo Fernandez-Megia, Ricardo A. Pires
Summary: In this study, the synthesis of nanosized, functional gallic acid-based dendrimers is described, which interact with Aβ species and remodel them into noncytotoxic aggregates. The multivalent presentation of gallic acid on the dendrimer surface enhances their ability to interact with Aβ, inhibiting fibrillization and disrupting preformed fibrils.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Nutrition & Dietetics
Lifang Zhang, Jessica Cao, Haiqiang Yang, Phillip Pham, Umer Khan, Breanna Brown, Yanhong Wang, Tarek Zieneldien, Chuanhai Cao
Summary: Coffee consumption, regardless of brand or type, is beneficial for stalling the progression and development of A beta-related Alzheimer's disease (AD). All coffees tested have beneficial health effects for AD through lowering A beta 1-40 and A beta 1-42 production, with Dunkin' Donuts (R) medium roast coffee demonstrating the most consistent and optimal cell survival rates and A beta concentration, while Starbucks (R) coffee exhibited the highest cell toxicity rates among the tested coffees.
FRONTIERS IN NUTRITION
(2022)
Article
Geriatrics & Gerontology
Chiara Zecca, Giuseppe Pasculli, Rosanna Tortelli, Maria Teresa Dell'Abate, Rosa Capozzo, Maria Rosaria Barulli, Roberta Barone, Miriam Accogli, Serena Arima, Alessio Pollice, Vincenzo Brescia, Giancarlo Logroscino
Summary: The study explores the influence of age and other factors on A beta(1-42) plasma levels in cognitively and neurologically normal individuals. Age and cholesterol were significantly associated with A beta(1-42) plasma level, with only the association of age surviving post hoc adjustment for multiple comparisons. Further research is needed to understand the age-dependent variability of A beta levels across different age groups.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Yoshie Haratake, Kohei Sano, Miki Tsuchiya, Kaori Minaki, Masayuki Munekane, Toshihide Yamasaki, Masayori Hagimori, Takahiro Mukai
Summary: This study designed and synthesized a novel radiolabeled amyloid imaging probe, demonstrating its potential for diagnosing amyloidosis.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Patricia Lam, Chitra Vinnakota, Beatriz Calvo-Flores Guzman, Julia Newland, Katie Peppercorn, Warren P. Tate, Henry J. Waldvogel, Richard L. M. Faull, Andrea Kwakowsky
Summary: Alzheimer's disease is a neurodegenerative disorder that requires the development of disease-modifying treatments. Recent evidence suggests that the GABA neurotransmitter system undergoes remodeling in AD, disrupting the excitatory/inhibitory balance in the brain. This study explores the expression levels of KCC2 and NKCC1 in AD and finds that chronic exposure to beta-amyloid alters the balance of KCC2 and NKCC1 expression in mouse hippocampal tissue. Furthermore, the study reveals that the drug bumetanide, used for AD therapy, is highly neurotoxic.
Article
Neurosciences
Mari Aksnes, Hans Christian D. Aass, Ann Tiiman, Lars Terenius, Nenad Bogdanovi, Vladana Vukojevi, Anne-Brita Knapskog
Summary: In this small pilot study, there was no association between serum nanoplaques and serum cytokines in patients assessed at a memory clinic. This suggests that serum nanoplaque levels cannot be used to differentiate clinical AD patients from non-AD patients in this unselected memory clinic cohort.
JOURNAL OF ALZHEIMERS DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Kazuma Murakami, Shiori Horii, Mizuho Hanaki, Kazuhiro Irie
Summary: The aggregation of Amyloid beta 42 in Alzheimer's disease involves nucleation and elongation phases. An orcein derivative, O4, has been shown to convert toxic oligomers into inert fibrils. Screening natural products for compounds that delay nucleation and promote elongation revealed several candidates that reduce the toxicity of Amyloid beta 42 against neuroblastoma cells. These compounds may interact with Amyloid beta 42 to shift its equilibrium from toxic oligomers to inert fibrils.
ACS CHEMICAL NEUROSCIENCE
(2021)
Letter
Biochemistry & Molecular Biology
Christopher Exley
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
(2020)
Letter
Environmental Sciences
Emma Shardlow, Matthew Mold, Christopher Exley
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Farhang Aliakbari, Hossein Mohammad-Beigi, Shahsanam Abbasi, Nasrollah Rezaei-Ghaleh, Frederik Lermyte, Soha Parsafar, Stefan Becker, Azita Parvaneh Tafreshi, Peter B. O'Connor, Joanna F. Collingwood, Gunna Christiansen, Duncan S. Sutherland, Poul Henning Jensen, Dina Morshedi, Daniel E. Otzen
Summary: Incorporation of baicalein into zwitterionic nanoliposomes successfully addresses the stability and solubility issues, making NLP-Ba effective in inhibiting alpha-synuclein fibrillation associated with Parkinson's disease. This nanosystem shows potential neuroprotective effects and could be a promising therapeutic option against PD.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Clinical Neurology
Simone M. Crivelli, Qian Luo, Jo A. A. Stevens, Caterina Giovagnoni, Daan van Kruining, Gerard Bode, Sandra den Hoedt, Barbara Hobo, Anna-Lena Scheithauer, Jochen Walter, Monique T. Mulder, Christopher Exley, Matthew Mold, Michelle M. Mielke, Helga E. De Vries, Kristiaan Wouters, Daniel L. A. van den Hove, Dusan Berkes, Maria Dolores Ledesma, Joost Verhaagen, Mario Losen, Erhard Bieberich, Pilar Martinez-Martinez
Summary: The study reveals that CERTL binds to APP, modifies Aβ aggregation, and reduces Aβ neurotoxicity in vitro. In a mouse model of familial AD, overexpression of CERTL decreases Aβ levels, modulates microglia, and has mild effects on animal locomotion. These findings suggest a crucial role of CERTL in regulating ceramide levels in the brain and in the pathogenesis of AD and other neurodegenerative diseases.
ALZHEIMERS RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Emma Shardlow, Caroline Linhart, Sameerah Connor, Erin Softely, Christopher Exley
Summary: The study measured the aluminium content of thirteen infant vaccines, finding that only three vaccines contained the amount indicated by the manufacturer. Six vaccines had a greater quantity of aluminium, while four vaccines had a lower quantity. The data raised questions about the significance of aluminium content in vaccines and highlighted areas of limited information. Monitoring the aluminium content in vaccines is crucial for ensuring both efficacy and safety, given the known toxicity of aluminium in humans.
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Emma Shardlow, Bakhtbilland Khan, Christopher Exley
Summary: This study investigated the impact of different metal ions on the aggregatory behavior and size of A beta(1-42) under simulated physiological conditions. The results showed that Al3+ ions stabilized smaller, toxic intermediates while excess Cu2+ facilitated the formation of larger, more inert species. This suggests a complex relationship between metal ions and the toxicity of A beta(1-42).
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Matthew John Mold, Christopher Exley
Summary: This study found that aluminium co-localises with lipid-rich Biondi ring tangles in the choroid plexus of Parkinson's disease donors. Although Biondi ring tangles are not composed of phosphorylated tau, the latter was identified in the nuclei of choroidal cells where aluminium and Biondi ring tangles co-localised.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Alberto Falco, Melissa Bello-Perez, Rocio Diaz-Puertas, Matthew Mold, Mikolaj Adamek
Summary: Different inactivation procedures were tested for developing an inactivated vaccine against a new deadly NNV isolate affecting European seabass, with UV-C irradiation and formalin treatment showing promising results as potential NNV-inactivation procedures for vaccine candidates.
Review
Biochemistry & Molecular Biology
Yunxing Ma, Julia Brettschneider, Joanna F. Collingwood
Summary: Levels of amyloid-beta and tau in human cerebrospinal fluid were evaluated to predict the transition from Mild Cognitive Impairment to Alzheimer's disease. The results support the value of amyloid-beta and tau as markers of patient outcome.
Article
Biochemistry & Molecular Biology
Caroline Silveira Martinez, Jose Antonio Uranga-Ocio, Franck Maciel Pecanha, Dalton Valentim Vassallo, Christopher Exley, Marta Miguel-Castro, Giulia Alessandra Wiggers
Summary: The study found that egg white hydrolysate (EWH) can protect rats from the effects of long-term exposure to high and low levels of aluminum in the diet on reproductive function. The supplementation of EWH can reduce inflammation and oxidative stress in the reproductive organs, and maintain normal sperm production and quality.
Article
Neurosciences
Matthew John Mold, Adam O. Farrell, Benjamin Morris, Christopher Exley
Summary: The study found that aluminum is intricately associated with amyloid-beta and neurofibrillary tangles in the brains of donors with Familial Alzheimer's disease. Aluminum was more frequently co-deposited with cortical senile plaques than with neurofibrillary tangles, suggesting a potential preference for binding with amyloid-beta. The data support the role of aluminum in the neuropathology of fAD and suggest that reducing aluminum may have therapeutic benefits.
JOURNAL OF ALZHEIMERS DISEASE REPORTS
(2021)
Article
Chemistry, Inorganic & Nuclear
James Everett, Jake Brooks, Joanna F. Collingwood, Neil D. Telling
Summary: Iron is a vital trace element for healthy brain function, but its disrupted neurochemistry and interaction with beta-amyloid (Aβ) have been implicated in the development of degenerative brain disorders like Alzheimer's disease (AD). This study utilized nanoscale resolution imaging to observe the variations in iron oxidation state and the organic composition of Aβ, demonstrating a potential link between altered iron chemistry and neurodegenerative processes in AD.
INORGANIC CHEMISTRY FRONTIERS
(2021)
Article
Chemistry, Multidisciplinary
Jane M. Donnelly, Frederik Lermyte, Juliusz A. Wolny, Marc Walker, Ben G. Breeze, Russell J. Needham, Christina S. Muller, Peter B. O'Connor, Volker Schunemann, Joanna F. Collingwood, Peter J. Sadler
Summary: A stable square-planar Cu(iii) complex, [bis(toluene-3,4-dithiolato)copper(iii)][NEt3H], has been synthesised and characterised using various analytical techniques. Interestingly, a unique [(toluene-3,4-dithiolato)Cu(iii)(peroxide)](-) complex is formed when the complex is fragmented in FTICR-MS upon reaction with oxygen. Additionally, natural 1,2-dithiolenes known to bind molybdenum may play a role in stabilising Cu(iii) in vivo.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Frederik Lermyte, Wen-Ying Zhang, Jake Brooks, Steven Huband, Joanna F. Collingwood, Martin R. Lees, Margaret P. Rayman, Peter J. Sadler
Article
Multidisciplinary Sciences
Christopher Exley, Matthew J. Mold