4.7 Article

Physicochemical and Flavor Characteristics of Flavoring Agent from Mungbean Protein Hydrolyzed by Bromelain

Journal

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 59, Issue 15, Pages 8475-8483

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/jf202006a

Keywords

physicochemical; bromelain; flavoring agents; protein hydrolysate; mungbean meal protein isolate; volatile compounds

Funding

  1. Thailand Research Fund Master Research Grants (TRF-MAG)
  2. Higher Education Research Promotion
  3. National Research University Project of Thailand
  4. King Mongkut's University of Technology Thonburi, Thailand

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Enzymatic bromelain mungbean meal protein hydrolysate (eb-MPH) was produced from mungbean meal protein isolate (MPI). Enzymatic bromelain, with a known protease activity of 98,652 (unit/g), was used at concentrations of 0, 2, 6, 10, 14 and 18% (w/w) and with hydrolysis times of 0.5, 3, 6, 12, and 24 h. The pH and temperature were controlled at 6.0 and 50 degrees C, respectively. It was found that the best treatment combination for eb-MPH production by response surface methodology (RSM) was 18% bromelain and a hydrolysis time of 3 h, resulting in the greatest degree of hydrolysis (% DH) and percent yield, with values of 61.04 and 45.63%, respectively. Results also showed that the phenylalanine, tyrosine and leucine contents of the optimally produced eb-MPH were 20.88, 14.50 and 10.93%, respectively. Twelve volatile compounds were identified using gas chromatography mass spectrometry in eb-MPH; benzaldehyde, 2-pentylfuran and furfural were the predominant odorants.

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