Article
Biochemistry & Molecular Biology
Hanrui Qing, Xueting Huo, Shimeng Huang, Lihong Zhao, Jianyun Zhang, Cheng Ji, Qiugang Ma
Summary: The study demonstrated that Bacillus subtilis ANSB168 could degrade OTA and its bioproduct could alleviate damages induced by OTA in laying hens, including reducing inflammation and oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Christoph Gonaus, Laura Wieland, Gerhard G. Thallinger, Shreenath Prasad
Summary: Ochratoxin A, a mycotoxin produced by certain fungi, poses a threat to animal and human health due to its nephrotoxic, carcinogenic, and genotoxic properties. Stenotrophomonas sp. 043-1a, a bacterium isolated from soil, has been previously shown to degrade ochratoxin A into non-toxic compounds. In this study, the enzymes responsible for this degradation were identified as the metallo-dependent hydrolase Chr1_3858681_3267 and a member of the peptidase S9 family, Chr1_3858681_771. This is the first report of an ochratoxin A degrading enzyme from the peptidase S9 family.
FEMS MICROBIOLOGY LETTERS
(2023)
Article
Agriculture, Dairy & Animal Science
Insaf Riahi, Antonio J. Ramos, Jog Raj, Zdenka Jakovcevic, Hunor Farkas, Marko Vasiljevic, Anna Maria Perez-Vendrell
Summary: The contamination of animal feed with mycotoxins is a global concern, leading to adverse effects on productivity and animal health. The addition of detoxifying agents, such as MMDA, to contaminated diets can alleviate the negative impact of mycotoxins on broiler chickens.
Article
Chemistry, Analytical
Zahra Khoshbin, Elham Sameiyan, Hamed Zahraee, Mohammad Ramezani, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: In this study, a simple aptasensing approach was used to detect ochratoxin A (OTA), a dangerous mycotoxin with severe health effects. The aptasensor utilized the alteration of liquid crystal (LC) molecules at the surfactant-arranged interface to sensitively detect OTA. By perturbing the alignment of LCs, the presence of OTA induced a noticeable change in the polarized view of the aptasensor substrate. The aptasensor demonstrated high sensitivity and could detect OTA in various real samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Food Science & Technology
Majid Behfar, Ali Heshmati, Freshteh Mehri, Amin Mousavi Khaneghah
Summary: This study used the response surface methodology to achieve maximum removal of ochratoxin A (OTA) while minimizing reduction in antioxidant compounds during the grape juice clarification process. The results showed that the concentrations of bentonite, gelatin, and diatomite, as well as the clarification time, were correlated with the reduction amounts of OTA and antioxidant compounds. The study also found that the reduction amounts of OTA and antioxidant capacity followed a linear mode, while the decreased phenolic acid and flavonoid values followed a quadratic model.
Article
Food Science & Technology
Maria Izco, Ariane Vettorazzi, Maria de Toro, Yolanda Saenz, Lydia Alvarez-Erviti
Summary: The study showed that sub-chronic exposure to low doses of OTA significantly altered the structure and diversity of the gut microbiome in mice, with even more pronounced effects than in the high-dose OTA group.
Article
Food Science & Technology
Beyza H. Ulusoy, Canan Hecer, Serkan Sayiner, Fatma Kaya Yildirim
Summary: Dietary exposure to mycotoxins in samarella, a traditional meat product in Cyprus, poses a risk to public health, with some samples exceeding the safety limits for total aflatoxins and AFB1, while no contamination was found for OTA.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2022)
Review
Chemistry, Applied
Abirami Ramu Ganesan, Kannan Mohan, Durairaj Karthick Rajan, Arti A. Pillay, Thavamani Palanisami, Palanivel Sathishkumar, Lorenza Conterno
Summary: Mycotoxins, such as OTA and DON, are widely distributed in food materials and have harmful effects on human health. Improper handling and practices during the production and processing of food can lead to the formation of these mycotoxins. Understanding their distribution, toxicity, and interaction with other contaminants is crucial for ensuring food safety and good agricultural practices.
Article
Biochemistry & Molecular Biology
Jelena Loncar, Barbara Bellich, Alessia Parroni, Massimo Reverberi, Roberto Rizzo, Slaven Zjalic, Paola Cescutti
Summary: The study evaluated oligosaccharides extracted from Trametes versicolor for controlling two mycotoxins, with heptasaccharides showing up to 90% inhibitory activity. These results suggest that these oligosaccharides could be natural alternatives for controlling mycotoxins.
Article
Spectroscopy
Qin Ma, Dongqin Nie, Xinyi Sun, Yaling Xu, Jingxian He, Li Yang, Lizhu Yang
Summary: A versatile Y shaped DNA nanostructure has been developed for simultaneous detection of aflatoxin B1 and ochratoxin A. The method is simple and rapid, utilizing self-powered DNA hybridization reaction to control the opening of DNA tweezers, allowing for quantitative detection of target mycotoxins.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Biochemistry & Molecular Biology
Ana Lucia Leitao, Francisco J. Enguita
Summary: Through structure-based methods and proteome mining, potential enzymatic activity capable of degrading ochratoxins has been identified in filamentous fungi, offering a promising solution for detoxification of various food commodities.
Article
Pharmacology & Pharmacy
Riddhi Sharma, Sean M. Gettings, Gareth Hazell, Nora Bourbia
Summary: This study investigated the impact of Ochratoxin A (OTA) on neuronal survival and viability. The findings suggest that typical daily exposure to OTA may not trigger neuronal dysfunction, but increased OTA dose caused decreased BDNF expression, potentially leading to adverse effects on neuronal health.
Review
Food Science & Technology
Houshmand Sharafi, Negar Alizadeh, Mehran Moradi, Kiomars Sharafi, Amin Mousavi Khaneghah
Summary: The prevalence of ochratoxin A (OTA) in meat, edible offal, and meat products (MOP) was assessed through systematic review and meta-analysis. A total of 75 articles comprising 8585 samples from global studies were analyzed. The overall prevalence of OTA in MOP was 39%, with the highest and lowest prevalence percentages recorded in Iraq (77%) and the USA (3%), respectively. The study also found variations in OTA concentration across different food types and countries.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Food Science & Technology
Z. Dilek Heperkan, Ece Gunalan-Inci, Tugce Ceyhan
Summary: Fruit juices, dried fruits, and other fruit-based products contribute significantly to human exposure to mycotoxins. This study examined the contamination of patulin (PAT) and ochratoxin A (OTA) in homemade vinegar made from various fruits, with apples and grapes being the most contaminated. Analysis indicated that 61% of the vinegar samples were contaminated with PAT, and 12% with OTA. Interestingly, vinegar produced from fruits collected from the producers' own orchards showed the highest levels of mycotoxin contamination. This contamination poses a health risk, as 15% of the vinegar samples exceeded the European Union limit for PAT concentration.
Article
Biotechnology & Applied Microbiology
Meilin Wei, Solairaj Dhanasekaran, Guillaume Legrand Ngolong Ngea, Esa Abiso Godana, Xiaoyun Zhang, Qiya Yang, Xiangfeng Zheng, Hongyin Zhang
Summary: The study found that Cryptococcus podzolicus Y3 efficiently eliminated ochratoxin A (OTA) and converted it into non-toxic ochratoxin A (OTa) in different media. The elimination substances produced by the strain were heat stable and involved intracellular proteins. Additionally, C. podzolicus Y3 also had the ability to degrade citrinin (CIT), although the efficiency was lower than single mycotoxin degradation.
BIOLOGICAL CONTROL
(2022)
Article
Biochemistry & Molecular Biology
Diego Barriales, Itziar Martin-Ruiz, Ana Carreras-Gonzalez, Marta Montesinos-Robledo, Mikel Azkargorta, Ibon Iloro, Iraide Escobes, Teresa Martin-Mateos, Estibaliz Atondo, Ainhoa Palacios, Monika Gonzalez-Lopez, Laura Barcena, Ana R. Cortazar, Diana Cabrera, Ainize Pena-Cearra, Sebastiaan M. van Liempd, Juan M. Falcon-Perez, Miguel A. Pascual-Itoiz, Juana Maria Flores, Leticia Abecia, Aize Pellon, Maria Luz Martinez-Chantar, Ana M. Aransay, Alberto Pascual, Felix Elortza, Edurne Berra, Jose Luis Lavin, Hector Rodriguez, Juan Anguita
Summary: Lyme carditis is associated with down-regulation of mitochondrial components in the heart and long-term memory-like responses in macrophages exposed to B. burgdorferi. This memory-like response can be manipulated in vivo by inhibiting glycolysis, leading to reduced inflammation and spirochetal burdens, and the recovery of tissue mitochondrial components.
Article
Biotechnology & Applied Microbiology
Jose Miguel Mancheno, Estibaliz Atondo, Julen Tomas-Cortazar, Jose Luis Lavin, Laura Plaza-Vinuesa, Itziar Martin-Ruiz, Diego Barriales, Ainhoa Palacios, Claudio Daniel Navo, Leticia Sampedro, Ainize Pena-Cearra, Miguel Angel Pascual-Itoiz, Janire Castelo, Ana Carreras-Gonzalez, Donatello Castellana, Aize Pellon, Susana Delgado, Patricia Ruas-Madiedo, Blanca delas Rivas, Leticia Abecia, Rosario Munoz, Gonzalo Jimenez-Oses, Juan Anguita, Hector Rodriguez
Summary: The occurrence and development of colorectal cancer is associated with the presence of Fusobacterium nucleatum, dietary components, and reduction of acetylated derivatives of spermidine. The study found that a new tannase orthologue of F. nucleatum (TanBF(nn)) has structural differences affecting the active site and substrate accessibility. Spermidine and its derivatives inhibit the hydrolytic activity of TanBF(nn) and increase the toxicity of gallotannins to F. nucleatum.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Eduardo Rosado, Paloma Delgado-Fernandez, Blanca de las Rivas, Rosario Munoz, Francisco Javier Moreno, Nieves Corzo, Cesar Mateo
Summary: This study presents the synthesis of beta-galactosyl xylitol derivatives using immobilized LacA beta-galactosidase from Lactobacillus plantarum WCFS1. The immobilized enzyme exhibited high activity and stability, allowing for the synthesis of a large amount of beta-galactosyl xylitol derivatives with improved efficiency. It also showed lower in vitro intestinal digestibility compared to lactose.
Review
Biochemistry & Molecular Biology
Felix Lopez de Felipe, Blanca de las Rivas, Rosario Munoz
Summary: This review summarizes the molecular mechanisms involved in the responses of Lactobacillus spp. to plant phenolic compounds (PPCs). Lactobacilli are more tolerant to PPCs compared to other bacteria and induce a wide variety of mechanisms to increase oxidative stress response. Additionally, PPCs can modulate molecular functions used by lactobacilli to adapt to host environments.
Article
Engineering, Chemical
Antonella Costantini, Enrico Vaudano, Laura Pulcini, Lara Boatti, Elisa Gamalero, Emilia Garcia-Moruno
Summary: The study focused on monitoring the evolution of yeast microflora on the berry surface during the ripening of Barbera grapes. Different methodologies, including culture-dependent methods and NGS sequencing, were employed. The results showed a variety of yeast species present at different stages of ripening, highlighting the importance of using integrated techniques for a comprehensive understanding of mycodiversity in the wine-growing environment.
Article
Gastroenterology & Hepatology
Maria Juarez-Fernandez, Naroa Goikoetxea-Usandizaga, David Porras, Maria Victoria Garcia-Mediavilla, Miren Bravo, Marina Serrano-Macia, Jorge Simon, Teresa C. Delgado, Sofia Lachiondo-Ortega, Susana Martinez-Florez, Oscar Lorenzo, Mercedes Rincon, Marta Varela-Rey, Leticia Abecia, Hector Rodriguez, Juan Anguita, Esther Nistal, Maria Luz Martinez-Chantar, Sonia Sanchez-Campos
Summary: This study provides evidence for the relevance of the interaction between mitochondria and microbiota in NASH and identifies targeting this interaction as a valuable therapeutic approach.
Article
Agriculture, Multidisciplinary
Laura Plaza-Vinuesa, Oswaldo Hernandez-Hernandez, Ana Sanchez-Arroyo, Jose M. Cumella, Nieves Corzo, Ana M. Munnoz-Labrador, F. Javier Moreno, Blanca de las Rivas, Rosario Munoz
Summary: This study confirmed the myrosinase-like activity of Lactiplantibacillus plantarum WCFS1, indicating that glucosinolates in this strain may undergo phosphorylation and the glycosidases belonging to the GH1 family are involved in the hydrolysis of phosphorylated glucosinolates.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Immunology
Michelle J. Klouwens, Jos J. A. Trentelman, Diego Barriales, Jasmin I. Ersoz, Mikel Azkargorta, Felix Elortza, Radek Sima, Ondrej Hajdusek, Julen Tomas Cortazar, Iraide Escobes Corcuera, Emil Colstrup, Abhijeet Nayak, Itziar Martin Ruiz, Hector Rodriguez, Ard M. Nijhof, Juan Anguita, Joppe W. R. Hovius
Summary: In this study, differential protein production in the salivary glands of Ixodes ricinus ticks in response to Borrelia afzelii infection and different feeding conditions was identified using quantitative proteomics. Recombinant tick proteins selected from this study significantly reduced the post-engorgement weights of I. ricinus nymphs in experimental animal models, but efficient transmission of B. afzelii to the murine host was still observed.
Article
Biotechnology & Applied Microbiology
Ainize Pena-Cearra, Deguang Song, Janire Castelo, Ainhoa Palacios, Jose Luis Lavin, Mikel Azkargorta, Felix Elortza, Miguel Fuertes, Miguel Angel Pascual-Itoiz, Diego Barriales, Itziar Martin-Ruiz, Asier Fullaondo, Ana M. Aransay, Hector Rodriguez, Noah W. Palm, Juan Anguita, Leticia Abecia
Summary: There is potential association between mitochondrial dysfunction and inflammatory bowel diseases (IBD). The bidirectional interaction between the intestinal microbiota and host mitochondria may affect intestinal inflammation. The study shows that fecal microbiota transplantation (FMT) from MCJ-deficient mice to germ-free mice increases susceptibility to colitis. Furthermore, the study identifies potential microbial signatures associated with complex I deficiency and disease progression.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Article
Gastroenterology & Hepatology
Ainize Pena-Cearra, Janire Castelo, Jose Luis Lavin, Monika Gonzalez-Lopez, Miguel Angel Pascual-Itoiz, Miguel Fuertes, Virginia Gutierrez de Juan, Laura Barcena, Itziar Martin-Ruiz, Aize Pellon, Iratxe Seoane, Diego Barriales, Ainhoa Palacios, Asier Fullaondo, Iago Rodriguez-Lago, Maria L. Martinez-Chantar, Ana M. Aransay, Hector Rodriguez, Juan Anguita, Leticia Abecia
Summary: Anti-TNF therapy is effective in inducing and maintaining remission in intestinal bowel disease. However, a certain percentage of patients do not respond to this therapy. This study suggests that the absence of MCJ, a natural inhibitor of respiratory chain Complex I, leads to changes in gut microbiota, which is a critical determinant of the lack of response in a mouse model of inflammation. The study also highlights the role of macrophage mitochondrial function in the gut ecological niche in affecting the severity of inflammation and the response to current therapies.
Article
Multidisciplinary Sciences
Ainize Pena-Cearra, Miguel Angel Pascual-Itoiz, Jose Luis Lavin, Miguel Fuertes, Itziar Martin-Ruiz, Janire Castelo, Ainhoa Palacios, Diego Barriales, Asier Fullaondo, Ana M. Aransay, Hector Rodriguez, Juan Anguita, Leticia Abecia
Summary: Inflammatory bowel disease (IBD) is a complex and chronic disease. This study found that MCJ deficiency leads to increased TACE activity in chronic inflammation, affecting the metabolic adaptation of colon tissue and potentially associated with reduced microbial members associated with inflammation.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Carolina Simo, Tiziana Fornari, Monica R. Garcia-Risco, Ainize Pena-Cearra, Leticia Abecia, Juan Anguita, Hector Rodriguez, Virginia Garcia-Canas
Summary: In this study, a novel screening strategy was developed to evaluate the effects of food and natural extract on Klebsiella pneumoniae's TMA production. Oregano and red thyme extracts were found to have the potential to inhibit TMA formation.
