Journal
BIOMOLECULES
Volume 11, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/biom11071040
Keywords
mycotoxin; ochratoxin A; ochratoxinase; biodegradation; structural biology
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Funding
- Portuguese Foundation for Science and Technology (FCT) [PTDC-MED-GEN-29389-2017]
- Fundação para a Ciência e a Tecnologia [PTDC/MED-GEN/29389/2017] Funding Source: FCT
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Through structure-based methods and proteome mining, potential enzymatic activity capable of degrading ochratoxins has been identified in filamentous fungi, offering a promising solution for detoxification of various food commodities.
(1) Background: ochratoxins are mycotoxins produced by filamentous fungi with important implications in the food manufacturing industry due to their toxicity. Decontamination by specific ochratoxin-degrading enzymes has become an interesting alternative for the treatment of contaminated food commodities. (2) Methods: using a structure-based approach based on homology modeling, blind molecular docking of substrates and characterization of low-frequency protein motions, we performed a proteome mining in filamentous fungi to characterize new enzymes with potential ochratoxinase activity. (3) Results: the proteome mining results demonstrated the ubiquitous presence of fungal binuclear zinc-dependent amido-hydrolases with a high degree of structural homology to the already characterized ochratoxinase from Aspergillus niger. Ochratoxinase-like enzymes from ochratoxin-producing fungi showed more favorable substrate-binding pockets to accommodate ochratoxins A and B. (4) Conclusions: filamentous fungi are an interesting and rich source of hydrolases potentially capable of degrading ochratoxins, and could be used for the detoxification of diverse food commodities.
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