FRAP, FLIM, and FRET: Detection and analysis of cellular dynamics on a molecular scale using fluorescence microscopy

Germ-cell hub position in a heteropteran testis correlates with the sequence and location of spermatogenesis and production of elaborate sperm bundles

(2015) George J. Ott et al.   MOLECULAR REPRODUCTION AND DEVELOPMENT

The potential of label-free nonlinear optical molecular microscopy to non-invasively characterize the viability of engineered human tissue constructs

(2014) Leng-Chun Chen et al.   BIOMATERIALS

Tracking single molecules at work in living cells

(2014) Akihiro Kusumi et al.   Nature Chemical Biology

Wide-field time-correlated single-photon counting (TCSPC) lifetime microscopy with microsecond time resolution

(2014) Liisa M. Hirvonen et al.   OPTICS LETTERS

Abscisic acid dynamics in roots detected with genetically encoded FRET sensors

(2014) Alexander M Jones et al.   eLife

FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis

(2014) Rainer Waadt et al.   eLife

Fluid Shear Stress on Endothelial Cells Modulates Mechanical Tension across VE-Cadherin and PECAM-1

(2013) Daniel E. Conway et al.   CURRENT BIOLOGY

Vinculin tension distributions of individual stress fibers within cell-matrix adhesions

(2013) C.-W. Chang et al.   JOURNAL OF CELL SCIENCE

Small molecule inhibition of the KRAS–PDEδ interaction impairs oncogenic KRAS signalling

(2013) Gunther Zimmermann et al.   NATURE

N-Way FRET Microscopy of Multiple Protein-Protein Interactions in Live Cells

(2013) Adam D. Hoppe et al.   PLoS One

T Cell Activation Results in Conformational Changes in the Src Family Kinase Lck to Induce Its Activation

(2013) A. Stirnweiss et al.   Science Signaling

A Versatile Toolkit to Produce Sensitive FRET Biosensors to Visualize Signaling in Time and Space

(2013) R. D. Fritz et al.   Science Signaling

FRET microscopy in the living cell: Different approaches, strengths and weaknesses

(2012) Sergi Padilla-Parra et al.   BIOESSAYS

Minimizing the Impact of Photoswitching of Fluorescent Proteins on FRAP Analysis

(2012) Florian Mueller et al.   BIOPHYSICAL JOURNAL

NADH Distribution in Live Progenitor Stem Cells by Phasor-Fluorescence Lifetime Image Microscopy

(2012) Belinda K. Wright et al.   BIOPHYSICAL JOURNAL

Inhibition of Basal FGF Receptor Signaling by Dimeric Grb2

(2012) Chi-Chuan Lin et al.   CELL

Single Molecule Fluorescence Detection and Tracking in Mammalian Cells: The State-of-the-Art and Future Perspectives

(2012) Marisa Martin-Fernandez et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Total variation versus wavelet-based methods for image denoising in fluorescence lifetime imaging microscopy

(2012) Ching-Wei Chang et al.   Journal of Biophotonics

Orientation-based FRET sensor for real-time imaging of cellular forces

(2012) F. Meng et al.   JOURNAL OF CELL SCIENCE

Improving FRET dynamic range with bright green and red fluorescent proteins

(2012) Amy J Lam et al.   NATURE METHODS

Interplay between Cytoskeletal Stresses and Cell Adaptation under Chronic Flow

(2012) Deepika Verma et al.   PLoS One

The Scaffolding Protein Synapse-Associated Protein 97 Is Required for Enhanced Signaling Through Isotype-Switched IgG Memory B Cell Receptors

(2012) W. Liu et al.   Science Signaling

Fluorescence Correlation Spectroscopy: Past, Present, Future

(2011) Elliot L. Elson   BIOPHYSICAL JOURNAL

Biosensor Förster resonance energy transfer detection by the phasor approach to fluorescence lifetime imaging microscopy

(2011) Elizabeth Hinde et al.   MICROSCOPY RESEARCH AND TECHNIQUE

Development of an optimized backbone of FRET biosensors for kinases and GTPases

(2011) Naoki Komatsu et al.   MOLECULAR BIOLOGY OF THE CELL

Local, persistent activation of Rho GTPases during plasticity of single dendritic spines

(2011) Hideji Murakoshi et al.   NATURE

Quantitative evaluation of healthy epidermis by means of multiphoton microscopy and fluorescence lifetime imaging microscopy

(2011) Elisa Benati et al.   SKIN RESEARCH AND TECHNOLOGY

Three-Color Spectral FRET Microscopy Localizes Three Interacting Proteins in Living Cells

(2010) Yuansheng Sun et al.   BIOPHYSICAL JOURNAL

Enhancing precision in time-domain fluorescence lifetime imaging

(2010) Ching-Wei Chang et al.   JOURNAL OF BIOMEDICAL OPTICS

Visualizing dynamic cytoplasmic forces with a compliance-matched FRET sensor

(2010) F. Meng et al.   JOURNAL OF CELL SCIENCE

Assembly of the fluorescent acrosomal matrix and its fate in fertilization in the water strider, Aquarius remigis

(2010) Haruhiko Miyata et al.   JOURNAL OF CELLULAR PHYSIOLOGY

The design of Förster (fluorescence) resonance energy transfer (FRET)-based molecular sensors for Ran GTPase

(2010) Petr Kaláb et al.   METHODS

Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics

(2010) Carsten Grashoff et al.   NATURE

Precise fluorophore lifetime mapping in live-cell, multi-photon excitation microscopy

(2010) Ching-Wei Chang et al.   OPTICS EXPRESS

Instrumentation to rapidly acquire fluorescence wavelength-time matrices of biological tissues

(2010) William R. Lloyd et al.   Biomedical Optics Express

A Fast Multilevel Algorithm for Wavelet-Regularized Image Restoration

(2009) C. Vonesch et al.   IEEE TRANSACTIONS ON IMAGE PROCESSING

Physiological fluorescence lifetime imaging microscopy improves Förster resonance energy transfer detection in living cells

(2009) Ching-Wei Chang et al.   JOURNAL OF BIOMEDICAL OPTICS

Calibration and validation of an optical sensor for intracellular oxygen measurements

(2009) Dhruv Sud et al.   JOURNAL OF BIOMEDICAL OPTICS

Image analysis for denoising full-field frequency-domain fluorescence lifetime images

(2009) B.Q. SPRING et al.   JOURNAL OF MICROSCOPY

Physical and Functional Interaction between the Dopamine Transporter and the Synaptic Vesicle Protein Synaptogyrin-3

(2009) L. A. Egana et al.   JOURNAL OF NEUROSCIENCE

Coordination of Rho GTPase activities during cell protrusion

(2009) Matthias Machacek et al.   NATURE

Synchronous Hyperactivity and Intercellular Calcium Waves in Astrocytes in Alzheimer Mice

(2009) K. V. Kuchibhotla et al.   SCIENCE

Conformational changes of a Ca2+-binding domain of the Na+/Ca2+ exchanger monitored by FRET in transgenic zebrafish heart

(2008) Yi Xie et al.   AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY

Cyclic 3′,5′-AMP Causes ADAM1/ADAM2 to Rapidly Diffuse Within the Plasma Membrane of Guinea Pig Sperm1

(2008) Gary R. Hunnicutt et al.   BIOLOGY OF REPRODUCTION

Multiphoton microscopy and fluorescence lifetime imaging microscopy (FLIM) to monitor metastasis and the tumor microenvironment

(2008) Paolo P. Provenzano et al.   CLINICAL & EXPERIMENTAL METASTASIS

Engineering Redox-Sensitive Linkers for Genetically Encoded FRET-Based Biosensors

(2008) Vladimir L. Kolossov et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

A fluorescence energy transfer-based mechanical stress sensor for specific proteins in situ

(2008) Fanjie Meng et al.   FEBS Journal

Visualization of Polarized Membrane Type 1 Matrix Metalloproteinase Activity in Live Cells by Fluorescence Resonance Energy Transfer Imaging

(2008) Mingxing Ouyang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Regulation of chromatin binding by a conformational switch in the tail of the Ran exchange factor RCC1

(2008) Yi Hao et al.   JOURNAL OF CELL BIOLOGY

Rapid Frequency-Domain FLIM Spinning Disk Confocal Microscope: Lifetime Resolution, Image Improvement and Wavelet Analysis

(2008) Chittanon Buranachai et al.   JOURNAL OF FLUORESCENCE

Improving membrane voltage measurements using FRET with new fluorescent proteins

(2008) Hidekazu Tsutsui et al.   NATURE METHODS

Spatiotemporal activation of Rac1 for engulfment of apoptotic cells

(2008) M. Nakaya et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA