Article
Biochemistry & Molecular Biology
Ireneusz Litwin, Seweryn Mucha, Ewa Pilarczyk, Robert Wysocki, Ewa Maciaszczyk-Dziubinska
Summary: In this study, it was found that trivalent antimony can cause various forms of DNA damage, including replication and oxidative DNA damage, and affect the activation of DNA damage checkpoints and formation of recombination repair centers in the model organism Saccharomyces cerevisiae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Environmental Sciences
Sabrina Krief, Petra Spirhanzlova, Shelly Masi, Chloe Couturier, Eric Okwir, Edward Asalu, Paco Bustamante, David Costantini
Summary: Agricultural expansion leads to habitat loss and exposure to pesticides for non-human primates. This study analyzes the presence of pesticides in river water in the habitat range of wild chimpanzees in Uganda and investigates the relationship between pesticide exposure and oxidative damage. The results show that chimpanzees from high exposure areas have higher levels of oxidative DNA damage, and individuals with higher C-peptide levels also have higher oxidative DNA damage and lipid peroxides.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Genetics & Heredity
Paolo Pizzul, Erika Casari, Marco Gnugnoli, Carlo Rinaldi, Flavio Corallo, Maria Pia Longhese
Summary: This review focuses on the studies performed in Saccharomyces cerevisiae to define the checkpoint concept and identify its players and the mechanisms of activation and deactivation in DNA damage repair.
FRONTIERS IN GENETICS
(2022)
Article
Environmental Sciences
Marion Garofalo, Delphine Payros, Eric Oswald, Jean-Philippe Nougayrede, Isabelle P. Oswald
Summary: This study found that the dose of DON found in food can exacerbate DNA damage caused by etoposide, cisplatin, and phleomycin, but not the DNA damage caused by de-epoxy-deoxynivalenol (DOM-1). Ribotoxicity plays a key role in exacerbating DNA damage.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biology
Ehab Mustafa, Leonardo Makinistian, Jukka Luukkonen, Jukka Juutilainen, Jonne Naarala
Summary: The study revealed that exposure to 50 and 60 Hz magnetic fields may lead to a slight increase in expression of ROS-related genes, but had no significant effects on cytosolic O-2(center dot-) levels, DNA damage, and repair rate. Additionally, exposure to magnetic fields did not exceed the expected rate of false positive findings for changes in the expression of DNA damage-signaling genes.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2022)
Article
Toxicology
Ji-Eun Seo, Joshua Z. Yu, Hannah Xu, Xilin Li, Aisar H. Atrakchi, Timothy J. McGovern, Karen L. Davis Bruno, Nan Mei, Robert H. Heflich, Xiaoqing Guo
Summary: This study evaluated the genotoxicity of eight nitrosamines using metabolically competent human HepaRG cells. The HepaRG spheroids, which had higher levels of cytochrome P450 activity compared to monolayer cells, were used along with conventional 2D cultures. The results suggest that HepaRG spheroids may provide a sensitive, human-based cell system for evaluating the genotoxicity of nitrosamines.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Sneh M. Toprani, Dimitrios Bitounis, Qiansheng Huang, Nathalia Oliveira, Kee Woei Ng, Chor Yong Tay, Zachary D. Nagel, Philip Demokritou
Summary: The potential genotoxic effects of engineered nanomaterials (ENMs) on DNA repair pathways have been investigated using a fluorescence multiplex-host-cell reactivation (FM-HCR) assay. Results showed that some ENMs may enhance DNA repair capacity while others suppress it. This method can be a valuable part of a multitier, in vitro hazard assessment of ENMs, providing insights into the interplay between ENM properties, DNA repair efficiency, and genomic stability.
Article
Environmental Sciences
Felipe de Araujo Nascimento, Daniela de Melo e Silva, Thays Millena Alves Pedroso, Jheneffer Sonara Aguiar Ramos, Michelle Rocha Parise
Summary: Occupational exposure to pesticides in farmers results in significant DNA damage, showing a notable impact and variability in the studies. Smokers who are occupationally exposed to pesticides have slightly reduced DNA damage compared to non-smokers, indicating some genetic toxicity. The study demonstrates a higher risk of occupationally exposed rural workers developing related diseases due to the genotoxic effect of pesticides.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
F. Akcha, C. Cahuc, J. Rouxel, C. Munschy, Y. Aminot, T. Chouvelon, K. Mahe, H. Budzinski, A. Mauffret
Summary: Telomeres are suggested as a biomarker for cellular aging, stress exposure, and lifespan in humans, and a q-PCR protocol was used to measure Telomere Length (TL) in European flounder. The study found no correlation between TL and DNA damage or measured contaminant concentrations in the flounder, but further research is needed to determine the potential impact of chemical pollution on flatfish telomeres.
MARINE POLLUTION BULLETIN
(2021)
Article
Biochemistry & Molecular Biology
Aline H. Sousa, Joao P. G. Pereira, Allan C. Malaquias, Fernanda do E. S. Sagica, Edivaldo H. C. de Oliveira
Summary: Mercury is widely used in industrial processes and improper disposal of waste or products containing this metal can have significant negative impacts on ecosystems and living organisms, including humans. Methylmercury, a highly toxic organic compound, can cause neurological and developmental impairments. Recent research has shown that mercurial compounds can also cause genotoxic effects, such as disrupting mitotic spindle and causing chromosome loss, which are associated with the neurological effects of mercury. This study aimed to investigate the cytotoxic and genotoxic potential of methylmercury in neuron and glia cell lines. The findings showed that glial cells had a higher tolerance to methylmercury compared to neuronal cells, leading to higher intracellular accumulation of mercury and the occurrence of genotoxic effects.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ye Lv, Shuchang Chen, Bing Zhu, Hong Xu, Shanshan Xu, Weiyan Liu, Yunyun Shen, Qunli Zeng
Summary: The study found that 50 Hz ELF-MF did not induce DNA damage in FLs, HSFs, or HUVECs, regardless of the intensity or duration of exposure. This suggests that ELF-MF may not have significant effects on DNA damage in the tested cells.
BIOMED RESEARCH INTERNATIONAL
(2021)
Article
Biology
Satyaprakash Pandey, Mona Hajikazemi, Theresa Zacheja, Stephanie Schalbetter, Jonathan Baxter, Victor Guryev, Andreas Hofmann, Dieter W. Heermann, Stefan A. Juranek, Katrin Paeschke
Summary: The study found that the catalytic subunit of telomerase (Est2) in budding yeast binds to multiple guanine-rich genomic loci, known as non-telomeric binding sites (NTBS). Est2 binds to NTBS in G1 and G2 phase independently of Est1 and Est3. The absence of Est1 and Est3 renders telomerase inactive at NTBS.
Article
Environmental Sciences
Tuanwei Wang, Yu Meng, Yuting Tu, Guanghui Zhang, Kan Wang, Shiyang Gong, Yunxia Zhang, Tongshuai Wang, Anqi Li, David C. Christiani, William Au, Zhao-lin Xia
Summary: Studies have shown that lead exposure can cause genotoxicity, and one possible mechanism is through DNA methylation. This study aimed to investigate the association between DNA methylation, lead exposure, and genotoxicity. Blood lead levels and genotoxic biomarkers were analyzed in lead-exposed workers, and the methylation levels of specific genes were measured. The results showed that lead exposure induced changes in DNA methylation, which mediated lead-induced genotoxicity.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biochemistry & Molecular Biology
Galina N. Chelomina, Sergey P. Kukla, Viktor P. Chelomin, Pham N. Doanh
Summary: Parasite infection can lead to DNA damage, potentially involving oxidative stress and cancer development; these research findings are important for understanding the pathogenesis mechanisms of parasitic diseases.
Article
Biochemistry & Molecular Biology
Ievgeniia Gazo, Roman Franek, Radek Sindelka, Ievgen Lebeda, Sahana Shivaramu, Martin Psenicka, Christoph Steinbach
Summary: Sterlet embryos show varying sensitivity to different genotoxicants, with some inducing DNA damage and activating DDR pathways, while others causing DNA damage without activating known DDR pathways, with effects also being stage-specific in embryo development. This study provides foundational information for future investigation of sterlet DDR pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Environmental Sciences
Fernando Pareja-Pena, Antonio M. Burgos-Molina, Francisco Sendra-Portero, Miguel J. Ruiz-Gomez
Summary: Due to the increase in non-ionizing radiation emitting devices, concerns about their potential carcinogenicity have grown. Research suggests a possible causal relationship between RF-EMF exposure and brain tumor incidence, particularly with longer exposure and latency periods.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH
(2022)
Article
Environmental Sciences
Silvia Mercado-Saenz, Beatriz Lopez-Diaz, Antonio M. Burgos-Molina, Francisco Sendra-Portero, Alejandro Gonzalez-Vidal, Miguel Ruiz-Gomez
Summary: This study found that exposure of S. cerevisiae cells to pulsed magnetic field during chronological aging can lead to DNA damage and premature aging. An increase in spontaneous mutants was observed in the pulsed MF samples compared to unexposed controls, indicating genomic DNA damage induced by the exposure.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH
(2022)
Article
Education, Scientific Disciplines
Teodoro Rudolphi-Solero, Rocio Lorenzo-Alvarez, Miguel J. Ruiz-Gomez, Francisco Sendra-Portero
Summary: Competitive game-based learning within virtual worlds like Second Life shows great potential in teaching radiology, but when participation is compulsory, there is a decrease in scores, lower acceptance of virtual world technology, and poorer opinions about the game. Therefore, learning games in three-dimensional virtual environments should be voluntary to maintain motivation and engagement of medical students.
ANATOMICAL SCIENCES EDUCATION
(2022)
Article
Environmental Sciences
Alejandro Gonzalez-Vidal, Silvia Mercado-Saenz, Antonio M. Burgos-Molina, Francisco Sendra-Portero, Miguel J. Ruiz-Gomez
Summary: The study demonstrates that exposure to pulsed MF of 25Hz and 1.5mT for 33 hours can negatively impact the germination and growth of Allium cepa roots, resulting in a decrease in the number and length of roots grown.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH
(2022)
Review
Biology
Juan C. Alamilla-Presuel, Antonio M. Burgos-Molina, Alejandro Gonzalez-Vidal, Francisco Sendra-Portero, Miguel J. Ruiz-Gomez
Summary: Radiation resistance is a cause of treatment failure and tumor relapse. Understanding cancer stem cells and associated signaling pathways can lead to new strategies for radiation therapy. The mechanisms of radiation resistance in different tumors are not fully understood, and molecular markers can help classify and treat more aggressive tumors.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2022)
Article
Biology
Beatriz Lopez-Diaz, Silvia Mercado-Saenz, Antonio M. Burgos-Molina, Alejandro Gonzalez-Vidal, Francisco Sendra-Portero, Miguel J. Ruiz-Gomez
Summary: This study investigated the effect of pulsed magnetic field on genomic DNA damage induced by DNA damaging agents. The results showed that pulsed magnetic field increased the level of DNA damage caused by the agents.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2023)
Article
Environmental Sciences
Andres Barreiro-Ares, Annia Morales-Santiago, Francisco Sendra-Portero, Miguel Souto-Bayarri
Summary: The rise of artificial intelligence in medicine, particularly in radiology, has a significant impact on the practice and education model of the specialty. This study aims to analyze the perception of undergraduate medical students regarding the current situation of AI in medicine, specifically in radiology. A survey was conducted among 281 medical students, revealing that although 79.3% claimed to know what AI is, their objective knowledge about AI was low. Only 24.9% would choose radiology as a specialty, and only 40% included it in their top three choices. Despite that, most students recognized the positive value of AI applications in radiology and expressed no fear of being replaced by AI. Additionally, they emphasized the need for ethical principles in AI implementation and valued academic training in AI.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2023)
Article
Biochemistry & Molecular Biology
Alejandro Gonzalez-Vidal, Silvia Mercado-Saenz, Antonio M. Burgos-Molina, Juan C. Alamilla-Presuel, Miguel Alcaraz, Francisco Sendra-Portero, Miguel J. Ruiz-Gomez
Summary: The repair of genome and proteome damage caused by ionizing radiation, oxidizing agents, and aging is crucial for cellular homeostasis. This study investigates the relationship between resistance/response to ionizing radiation, cellular aging, and the mechanisms of response to oxidative stress, free radicals, reactive oxygen species (ROS), and antioxidant activity in yeast. The results identify several common processes, such as homologous recombination, non-homologous end joining, and base excision repair pathways, as essential for maintaining cellular homeostasis. The study also proposes common biomarkers for radiation resistance/response and aging, as well as for response to radiation and oxidative stress.
Article
Clinical Neurology
Jose P. Martinez-Barbero, Antonio J. Lainez-Ramos Bossini, Francisco Sendra-Portero
Summary: Headache is a common health issue, often requiring neuroimaging exams such as brain MRI to rule out secondary causes. This study found no significant association between headache-associated symptoms and detection of intracranial structural abnormalities in MRI studies.
REVISTA DE NEUROLOGIA
(2022)