Article
Biochemistry & Molecular Biology
Faye M. Vassel, Daniel J. Laverty, Ke Bian, Cortt G. Piett, Michael T. Hemann, Graham C. Walker, Zachary D. Nagel
Summary: Rev7 is a regulatory protein with multiple functions, including roles in TLS, DSB repair, replication fork protection, and cell cycle regulation. Dimerization of Rev7 is important for its roles in TLS, DSB repair, and regulation of the anaphase promoting complex, but partially dispensable for promoting mitotic spindle assembly through its interaction with Ran.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Kangying Wu, Qixing Zhou, Shaohu Ouyang
Summary: Graphene family nanomaterials have diverse applications and potential genotoxic effects on DNA, with mechanisms classified into direct and indirect genotoxicity. Factors such as physicochemical properties, exposure dose, and time influence the genotoxicity of GFNs, requiring further research on testing methods and response mechanisms.
Article
Biochemistry & Molecular Biology
Ireneusz Litwin, Seweryn Mucha, Ewa Pilarczyk, Robert Wysocki, Ewa Maciaszczyk-Dziubinska
Summary: In this study, it was found that trivalent antimony can cause various forms of DNA damage, including replication and oxidative DNA damage, and affect the activation of DNA damage checkpoints and formation of recombination repair centers in the model organism Saccharomyces cerevisiae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ievgeniia Gazo, Roman Franek, Radek Sindelka, Ievgen Lebeda, Sahana Shivaramu, Martin Psenicka, Christoph Steinbach
Summary: Sterlet embryos show varying sensitivity to different genotoxicants, with some inducing DNA damage and activating DDR pathways, while others causing DNA damage without activating known DDR pathways, with effects also being stage-specific in embryo development. This study provides foundational information for future investigation of sterlet DDR pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Engineering, Environmental
Ashiq Ahamed, Lili Liang, Ming Yang Lee, Johan Bobacka, Grzegorz Lisak
Summary: Engineered nanomaterials (ENMs) are a novel class of materials with superior physicochemical properties compared to conventional materials, but can potentially release into the environment and pose toxic effects. Research focuses on the transformations and potential risks of ENMs in consumer products, aiming to ensure their safe application.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Review
Nanoscience & Nanotechnology
Robert Landsiedel, Naveed Honarvar, Svenja Berit Seiffert, Barbara Oesch, Franz Oesch
Summary: Nanomaterials have unique advantages but may also have adverse effects on humans. However, traditional genotoxicity tests may not be suitable for nanomaterials and modifications are needed. This review critically examines available genotoxicity tests for nanomaterials and discusses their relative usefulness, as well as the open questions and further needs in adapting these tests.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Sarah May, Cordula Hirsch, Alexandra Rippl, Alexander Buerkle, Peter Wick
Summary: This study investigated the genotoxicity of different engineered nanomaterials (ENMs) using two complementary in vitro assays. The results showed that certain ENMs exhibited cytotoxicity and genotoxicity, while others did not have these effects.
Review
Cell Biology
Abhipsha Dey, Martin Flajshans, Martin Psenicka, Ievgeniia Gazo
Summary: Embryogenesis is a crucial stage in an organism's life, but externally fertilizing species like fish are particularly vulnerable to DNA damage from environmental factors. While DNA repair pathways have been extensively studied in some fish species, there is a lack of knowledge about these processes in non-model aquaculture fish. This review aims to summarize the features of different DNA repair pathways during fish embryo development, including their regulation, roles in organogenesis, and response to genotoxic stress, to establish a connection between genotoxic stress and embryo phenotype.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Maria Joao Bessa, Fatima Brandao, Paul H. B. Fokkens, Daan L. A. C. Leseman, A. John F. Boere, Flemming R. Cassee, Apostolos Salmatonidis, Mar Viana, Adriana Vulpoi, Simion Simon, Eliseo Monfort, Joao Paulo Teixeira, Sonia Fraga
Summary: Diverse industries have incorporated engineered nanoparticles (ENP) into their production processes, increasing the risk of worker inhalation exposure. In vitro models, particularly using air-liquid interface (ALI) cell cultures, are valuable for studying ENP toxicity. When comparing submerged and ALI conditions for ENP exposure, it was found that ALI cultures were more sensitive to the cytotoxic effects of ENP on human alveolar epithelial-like cells.
Article
Biochemistry & Molecular Biology
Le P. Ngo, Simran Kaushal, A. Isaac Chaim, Patrizia Mazzucato, Catherine Ricciardi, D. Leona Samson, D. Zachary Nagel, P. Bevin Engelward
Summary: By utilizing the CometChip, researchers uncovered significant variability in DNA repair efficiency among individuals, indicating its potential utility in future epidemiological and clinical studies.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Environmental Sciences
Marion Garofalo, Delphine Payros, Eric Oswald, Jean-Philippe Nougayrede, Isabelle P. Oswald
Summary: This study found that the dose of DON found in food can exacerbate DNA damage caused by etoposide, cisplatin, and phleomycin, but not the DNA damage caused by de-epoxy-deoxynivalenol (DOM-1). Ribotoxicity plays a key role in exacerbating DNA damage.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biotechnology & Applied Microbiology
Martina Fiumara, Samuele Ferrari, Attya Omer-Javed, Stefano Beretta, Luisa Albano, Daniele Canarutto, Angelica Varesi, Chiara Gaddoni, Chiara Brombin, Federica Cugnata, Erika Zonari, Matteo Maria Naldini, Matteo Barcella, Bernhard Gentner, Ivan Merelli, Luigi Naldini
Summary: Base and prime editors have shown potential for precise genetic engineering, but their cellular responses and genotoxicity are not well understood. This study compared the efficiency, toxicity, and genotoxicity between base and prime editors and Cas9 in human hematopoietic cells. The findings suggest that these editors can induce harmful effects and genotoxicity, raising concerns for their clinical applications.
NATURE BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Huishi Toh, Alexander Smolentsev, Ryan Sadjadi, Dennis Clegg, Jingqi Yan, Ron Stewart, James A. Thomson, Peng Jiang
Summary: Diabetic retinopathy is a common complication of long-term diabetes that can lead to vision loss. The early understanding and prevention of this condition are limited, with no effective methods until later stages. The study used a Nile rat model to examine the transcriptomic changes in retinal vascular development, aiming to better understand the early pathogenesis of diabetic retinopathy.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Huishi Toh, Alexander Smolentsev, Ryan Sadjadi, Dennis Clegg, Jingqi Yan, Ron Stewart, James A. Thomson, Peng Jiang
Summary: This study aims to understand the early pathogenesis of diabetic retinopathy by analyzing whole retinal vascular transcriptomic changes in a Nile rat model. The study identified the complex associations between acellular capillary density and factors like blood glucose, diet, and sex. A random forest regression model based on gene expression patterns was developed to predict acellular capillary density. Additionally, potential drugs that can attenuate early diabetic retinopathy were also identified.
SCIENTIFIC REPORTS
(2023)
Review
Endocrinology & Metabolism
Yan Wang, Mengrong Su, Yujie Chen, Xinyu Huang, Lian Ruan, Qizhuang Lv, Li Li
Summary: This study provides a systematic review of the role and mechanism of DNA damage repair in germ cell development. It summarizes the causes, detection methods, and repair methods of DNA damage, as well as the mechanism of DNA damage repair. It also discusses the causes of abnormal DNA damage repair in germ cells, the effects of germ cell damage, and the applications of DNA damage repair in reproductive diseases and related surgical treatments.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Nachiket Vaze, Anand R. Soorneedi, Matthew D. Moore, Philip Demokritou
Summary: This study developed an antimicrobial platform based on engineered water nanostructures (EWNS) using nanotechnology, which effectively inactivated the human coronavirus 229E (a surrogate of SARS-CoV-2) within 30 seconds, demonstrating its potential as a technology against SARS-CoV-2.
Article
Chemistry, Medicinal
Roxana Coreas, Carmen Castillo, Zongbo Li, Dong Yan, Ziting Gao, Junyi Chen, Dimitrios Bitounis, Dorsa Parviz, Michael S. Strano, Philip Demokritou, Wenwan Zhong
Summary: This study assessed the toxic effects of reduced graphene oxide (rGO) on mammary epithelial cells and found that rGO dissolved in sodium cholate may influence cellular toxicity. Surface-modified c-rGO exhibited higher toxicity, oxidative stress, and mitochondrial disruption compared to rGO, indicating a strong correlation between dispersant coating and cellular impacts.
CHEMICAL RESEARCH IN TOXICOLOGY
(2022)
Article
Engineering, Biomedical
Hui Ying Lai, Magdiel Inggrid Setyawati, Catarina Vizetto Duarte, Huei Min Chua, Choon Teck Low, Kee Woei Ng
Summary: Human hair proteins, especially keratins, are found to have high radical scavenging activity and can protect human dermal fibroblasts from oxidative stress.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Chelsea M. Cary, Glen M. DeLoid, Zhenning Yang, Dimitrios Bitounis, Marianne Polunas, Michael J. Goedken, Brian Buckley, Byron Cheatham, Phoebe A. Stapleton, Philip Demokritou
Summary: Recent studies have found that oral exposure to micro- and nano-plastics during pregnancy can have adverse effects on outcomes and progeny. This study examined the translocation of ingested nanoscale polystyrene MNPs to the placenta and fetal tissues in pregnant rats, and found that these MNPs can breach the intestinal and placental barriers to access the fetal circulation and all fetal tissues. Further research is needed to understand the mechanisms and potential adverse effects of this translocation.
Article
Chemistry, Multidisciplinary
Chin Guan Ho, Magdiel I. Setyawati, Glen M. DeLoid, Ke Li, Sunil S. Adav, Shuzhou Li, Say Chye Joachim Loo, Philip Demokritou, Kee Woei Ng
Summary: Exploitation of nature-derived materials is important for environmental sustainability, and cellulose is particularly interesting due to its abundance. Cellulose nanofibers (CNFs) have found applications as emulsifiers and lipid digestion modulators in food. This report demonstrates that CNFs can be modified to modulate toxin bioavailability in the gastrointestinal tract by forming inclusion complexes and promoting interaction with surface hydroxyl groups. Functionalized CNFs (FCNFs) were shown to effectively interact with the pesticide boscalid, and using an in vitro GIT simulation platform, it was found that FCNFs had a greater effect in retarding triglyceride digestion and binding boscalid in a simulated intestinal fluid environment. Overall, FCNFs have the potential to be developed into a functional food ingredient for modulating food digestion and toxin uptake.
Review
Cell Biology
Sunil S. Adav, Kee Woei Ng
Summary: Aging is a complex process that affects physiological functions, including hair graying, thinning, and loss. These changes in hair can have a significant impact on an individual's self-esteem and mental health. Omics technologies have helped lead to the discovery of hair aging biomarkers and potential therapies.
AGEING RESEARCH REVIEWS
(2023)
Article
Oncology
Rumani Singh, Stacey Yu, Marwa Osman, Zintis Inde, Cameron Fraser, Abigail H. Cleveland, Nicole Almanzar, Chuan Bian Lim, Gaurav N. Joshi, Johan Spetz, Xingping Qin, Sneh M. Toprani, Zachary Nagel, Matthew C. Hocking, Robert A. Cormack, Torunn I. Yock, Jeffrey W. Miller, Zhi-Min Yuan, Timothy Gershon, Kristopher A. Sarosiek
Summary: Age- and differentiation-dependent apoptotic priming plays a pivotal role in driving radiotherapy-induced neurocognitive impairment and can be targeted for neuroprotection in pediatric patients.
Article
Genetics & Heredity
Sunil S. Adav, Ching Yung Leung, Kee Woei Ng
Summary: This study utilized proteomic analysis to profile hair characteristics of individuals from different ethnic groups, genders, and ages, and found that hair proteins can be used for individual identification and differentiation.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2023)
Article
Green & Sustainable Science & Technology
Minh Phuong Do, Hong Kit Lim, Chiew Kei Tan, Ernest Jun Jie Tang, Madhavi Srinivasan, Chor Yong Tay
Summary: Fruit peel discards are used as a renewable waste biomass for lithium-ion battery recycling, achieving green chemical production. Extractive methods, including hot water treatment, ultrasonic-assisted hydrolysis, and fermentation, produce a fruit peel-derived lixiviant (FL) to extract valuable metals from spent LIB black mass. The FL obtained through fermentation using various fruit peels can leach over 90% of cobalt and lithium from LCO black mass, without synthetic chemicals. Additionally, oxalate precipitation can recover leached cobalt ions from orange peel-derived FL with a high yield of 85%. The regenerated cathode material coupled with LiOH supplementation exhibits excellent electrochemical performance with 93% capacity retention after 140 cycles. In conclusion, this FL-enabled LIB recycling method offers a significant opportunity for a more resource-efficient circular and sustainable economy.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Nanoscience & Nanotechnology
Dhimiter Bello, Lucia Chanetsa, Costas A. Christophi, Dilpreet Singh, Magdiel Inggrid Setyawati, David C. Christiani, Sanjay H. Chotirmall, Kee Woei Ng, Philip Demokritou
Summary: Inhalation of nanoparticles emitted from toner-based printing equipment (TPE), such as laser printers and photocopiers, has been associated with various health issues, including systemic inflammation, cardiovascular disease, and respiratory disorders. A study found dysregulated biomolecules related to inflammation and oxidative stress in TPE operators. Another study investigated the association between urinary oxidative stress biomarkers and nanoparticle exposure in the same TPE operators, finding significant associations with certain biomarkers. The combination of nanoparticle number concentration and oxidative stress potential is recommended for future studies.
Article
Materials Science, Multidisciplinary
Samyukta Ravishankar, Anu Maashaa Nedumaran, Archana Gautam, Kee Woei Ng, Bertrand Czarny, Sierin Lim
Summary: Nanoparticles, both organic and inorganic, are used as tools for diagnostic and therapeutic applications. However, there is limited understanding of the cellular fate and molecular responses of organic nanoparticles in macrophages. In this study, we investigated the intracellular fate and immune responses of a model organic nanoparticle, Archaeoglobus fulgidus ferritin, in murine macrophage cells.
NPG ASIA MATERIALS
(2023)
Article
Engineering, Environmental
Pujiang Shi, Tianle Huang, Hong Kit Lim, Chiew Kei Tan, Jong-Min Lee, Chor Yong Tay
Summary: This study developed a highly porous bioadaptive 3D sponge-like construct from plastic materials in discarded keyboards for advanced in vitro applications. The findings demonstrate the potential of using discarded keyboards as a waste-to-resource feedstock to achieve waste reduction and maximize value-capture.
RESOURCES CONSERVATION AND RECYCLING
(2024)
Article
Engineering, Environmental
Melissa Kao Hui Lee, Hong Kit Lim, Chengxun Su, Jie Yan Cheryl Koh, Magdiel Inggrid Setyawati, Kee Woei Ng, Han Wei Hou, Chor Yong Tay
Summary: This study used a three-dimensional biomimetic microfluidic lung-on-chip platform and RNA sequencing to investigate the effects of two model nanoparticles on airway epithelium cells. It was found that even low-level exposure to these nanoparticles triggered chemotaxis of lung fibroblasts and induced a transcriptomic response associated with the development of lung fibrosis. These findings provide new insights into the early acute events of respiratory harm caused by environmental nanoparticles exposure.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
