4.5 Article

Probiotic potential of lactic acid bacteria from fermented Malaysian food or milk products

Journal

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
Volume 47, Issue 10, Pages 2175-2183

Publisher

WILEY
DOI: 10.1111/j.1365-2621.2012.03085.x

Keywords

Fermented food; lactic acid bacteria; lactobacilli; milk; pediococci; probiotics

Funding

  1. Ministry of Science, Technology and Innovation Malaysia (MOSTI)

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This study investigated the probiotic characteristics of lactic acid bacteria (LAB) isolated from 14 Malaysian fermented food or milk products. In total, 22.3% (121 of 542) of the LAB isolated from the local fermented products exhibited antibacterial activity against Micrococcus luteus. Twelve LAB that demonstrated better antibacterial activity against M.similar to luteus as compared to a commercial strain Lactobacillus casei strain Shirota (LABPC) were selected for further characterisation. Based on 16S rRNA gene sequence, the LAB were identified as pediococci (seven) and lactobacilli (five). All 12 LAB showed bile tolerance, but only eight were acid tolerant at pH = 3.0. The highest level of adhesion to HT-29 cells was observed among the Lactobacillus sp. LAB 1 and 10. The LAB also showed antimicrobial activity against Escherichia coli and Staphylococcus aureus through the production of organic acids. LAB isolated from Malaysian fermented food and milk products, especially fermented tapioca, contains potential probiotic candidates.

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