4.3 Article

PHENOLIC PROFILE OF TUNISIAN OPUNTIA FICUS INDICA THORNLESS FORM FLOWERS VIA CHROMATOGRAPHIC AND SPECTRAL ANALYSIS BY REVERSED PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-UV-PHOTODIODE ARRAY AND ELECTROSPRAY IONIZATION-MASS SPECTROMETER

Journal

INTERNATIONAL JOURNAL OF FOOD PROPERTIES
Volume 17, Issue 4, Pages 741-751

Publisher

TAYLOR & FRANCIS INC
DOI: 10.1080/10942912.2012.665404

Keywords

Tunisian Opuntia ficus indica; Flowers of thornless form; Glycosylated flavonols; Isorhamnetin; Phenolic acids; ESI-MS

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The qualitative and quantitative analysis of phenolic compounds of the methanol extract of Tunisian thornless form Opuntia ficus indica flowers was described. Reversed phase high-performance liquid chromatography UV photodiode array analysis detected four peaks at 320 nm, attributed to phenolic acids; three of them were quantified to 141 mg.kg(-1) of fresh plant material. Electrospray ionization mass spectrometer analysis detected 8 glycosylated flavonols at 350 nm, identified among 11 chromatographic peaks. The amount of flavonoids was 4980 mg.kg(-1) of fresh plant material, with isorhamnetin glycosylated derivatives as the main flavonoid components (55.1%), quercetin derivatives as the second (23.7%), and kaempferol derivatives as the third (8.4%).

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