Article
Chemistry, Analytical
Ritong Sun, Jiao Chen, Yingeng Wang, Zheng Zhang, Yong Li, Fengmei Li, Cuiping Ma, Qingxia Han, Yanjing Shi
Summary: A highly sensitive and specific real-time narrow thermal-cycling amplification detection method based on accelerated strand exchange amplification (ASEA) was developed for the detection of Vibrio parahaemolyticus. The method can detect low concentrations of Vibrio parahaemolyticus in cultured samples and artificially spiked scallop meat within a short time. It is a useful tool for rapid detection of Vibrio parahaemolyticus in seafood samples.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Multidisciplinary
Yue Zhai, Xiangjun Meng, Li Li, Yushen Liu, Kun Xu, Chao Zhao, Juan Wang, Xiuling Song, Juan Li, Minghua Jin
Summary: This study presents a rapid and sensitive detection method for Vibrio parahaemolyticus by integrating magnetic nanobeads and quantum dots technology. The detection combines immunoseparation and immunofluorescence to achieve efficient detection of Vibrio parahaemolyticus, showing satisfactory results in real food samples.
Article
Food Science & Technology
Renpeng Li, Jing Wang, Lu Wang, Bin Hu, Zongdong Liu, Na Sun, Ying Yang, Song Miao, Xi Guo
Summary: In this study, nine untypeable K antigen strains of Vibrio parahaemolyticus were identified and characterized during a serotyping survey in Shandong province, China. Among these strains, three novel K serotypes were identified, with seven possessing the KUT2 antigens reported recently by another group. Comparative genomic analysis showed that the Shandong KUT2 strains exhibit different virulence profiles compared to their identical K serotype partners from Zhejiang province, suggesting possible evolution from a common ancestor. One isolate, SD2016062, was found to be phylogenetically similar to strains associated with local gastroenteritis outbreaks, indicating potential for disease spread. A sero-specific PCR assay targeting the three novel K serotypes was developed for clinical and epidemiological monitoring of V. parahaemolyticus strains.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Xueyun Chen, Liu Wang, Fang He, Ganghui Chen, Linlin Bai, Kaiyu He, Fang Zhang, Xiahong Xu
Summary: A novel label-free, colorimetric method for visual detection of V. parahaemolyticus was proposed, utilizing CRISPR/Cas12a to trim G-quadruplex DNAzyme and prevent color development of ABTS(2-). The method showed sensitivity comparable to real-time LAMP, detecting 6.1 x 10^2 CFU/mL V. parahaemolyticus in shrimp samples, making it a promising universal biosensing strategy for field pathogenic bacterial testing.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Mariam Almejhim, Mohammed Aljeldah, Nasreldin Elhadi
Summary: This study investigated the presence of toxigenic V. parahaemolyticus in coastal water in the Eastern Province of Saudi Arabia using IMS, chromogenic agar medium, and PCR. The results showed a high genomic diversity among the V. parahaemolyticus isolates, with some strains carrying toxin genes.
Article
Food Science & Technology
Monica Campas, Sandra Leonardo, Ainoa Ferre-Gode, Asif A. Chowdhury, Anna Toldra, Karl B. Andree, Ana Roque
Summary: Vibrio parahaemolyticus is a dominant cause of foodborne infections and can accumulate in shellfish. A duplex electrochemical biosensor was developed to detect the tdh and trh genes of V. parahaemolyticus in oysters, providing a quick and specific method for monitoring this pathogen.
Article
Food Science & Technology
Ali Parsaeimehr, Gulnihal Ozbay
Summary: We have developed a PCR-based detection platform integrated with HRPzyme and DNAzyme as a signaling probe to detect Vibrio parahaemolyticus. The colorimetric signal can detect bacterial colonies at concentrations as low as 101 cfu/mL. Extending the polyadenine length reduces background signaling and improves detection accuracy.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Haibo Zhou, Xinmei Liu, Zhaoxin Lu, Antuo Hu, Wenjie Ma, Changzheng Shi, Xiaomei Bie, Yiyu Cheng, Haijing Wu, Jun Yang
Summary: A new method called PMA-duplex ddPCR was developed for the quantitative determination of viable Vibrio parahaemolyticus in aquatic products, with lower limit of detection and high accuracy. This method allows for the detection of V. parahaemolyticus without pre-enrichment.
Article
Chemistry, Analytical
Wenhai Wang, Shu Xiao, Mengli Zeng, Hongzhen Xie, Ning Gan
Summary: Rapid and accurate detection of Vibrio parahaemolyticus (VP) is crucial for preventing the spread of foodborne illnesses. A dual-mode colorimetric-electrochemical biosensor was developed for the rapid and sensitive detection of VP. The biosensor achieved an accuracy of 88.7%, higher than that of single-mode assays (81.1%). It has the potential to facilitate effective on-site detection of pathogens in shrimp samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Cristina Rodriguez-Quijada, Casandra Lyons, Maria Sanchez-Purra, Charles Santamaria, Brianna M. M. Leonardo, Sara Quinn, Michael F. F. Tlusty, Michael Shiaris, Kimberly Hamad-Schifferli
Summary: Seafood contamination with Vibrio bacteria is a problem for aquaculture, especially with oysters. Current methods for diagnosing bacterial pathogens in seafood involve lab-based assays, which are time-consuming and must occur in a centralized location. A point-of-care assay that can detect the presence of Vibrio in a quick and convenient manner would be valuable for food safety control measures.
Article
Microbiology
Shengzhe Bian, Yangyang Jia, Qiuyao Zhan, Nai-Kei Wong, Qinghua Hu, Wenwei Zhang, Yongwei Zhang, Liqiang Li
Summary: VPsero is an in silico method for serotyping Vibrio parahaemolyticus strains using high-throughput sequencing technologies. It displays high specificity and sensitivity in prediction, covering 43 K and all 12 O serogroups.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biophysics
Hai Jiang, Zhikang Sun, Qian Guo, Xuan Weng
Summary: The thread-based microfluidic electrochemical aptasensor is designed for rapid and highly sensitive detection of Vibrio parahaemolyticus in seafood. It has higher detection sensitivity and shorter assay time compared to traditional methods.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biotechnology & Applied Microbiology
S. Ali, M. Hossain, A. B. Azad, A. B. Siddique, M. Moniruzzaman, M. A. Ahmed, M. B. Amin, M. S. Islam, M. M. Rahman, D. Mondal, Z. H. Mahmud
Summary: The study identified diverse Vibrio parahaemolyticus in marine fishes from local markets in Bangladesh with antibiotic-resistant properties and biofilm formation capacity. Enhanced monitoring is necessary to ensure the safety of seafood consumers.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Food Science & Technology
Dan Wang, Graham C. Fletcher, Stephen L. W. On, Jon S. Palmer, Dragana Gagic, Steve H. Flint
Summary: This study evaluated the biofilm formation ability of Vibrio parahaemolyticus isolated from seafood related environments and found that biofilm cells were less susceptible to sodium hypochlorite. Polysaccharide production was found to be possibly correlated with sodium hypochlorite resistance. Genetic diversity played a role in the variation of biofilm formation between isolates.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Food Science & Technology
Yuwei Ren, Lulu Cao, Xiyan Zhang, Rui Jiao, Dexin Ou, Yang Wang, Danfeng Zhang, Yizhong Shen, Na Ling, Yingwang Ye
Summary: In this study, a novel aptamer-based paper sensor was developed for the rapid, sensitive, portable, and specific detection of Vibrio parahaemolyticus in seafood. The sensor utilized the fluorescence resonance energy transfer (FRET) principle and demonstrated successful detection of the target bacteria. The results indicate the potential of this method as a new portable sensor for the detection of V. parahaemolyticus in complex food matrices.
Article
Immunology
Shuangshuang Ma, Lingyan Jiang, Jingting Wang, Xiaoqian Liu, Wanwu Li, Shuai Ma, Lu Feng
Summary: Salmonella enterica serovar Typhimurium utilizes host macrophages as a niche for survival and replication, controlling the expression of flagellar genes to minimize host immune response. The transcriptional regulator AsiR positively regulates flagellar gene expression and its downregulation contributes to S. Typhimurium replication and systemic infection. Acidic pH in macrophages serves as a signal for AsiR and flagellar gene downregulation.
Article
Chemistry, Applied
Xiaolong Jiang, Jing Bai, Jian Yuan, Huijing Zhang, Gege Lu, Yuhui Wang, Lingyan Jiang, Bin Liu, Lei Wang, Di Huang, Lu Feng
Summary: By reprogramming the metabolic network, the research team increased the yield of OPS-based glycoconjugate vaccines to 38.6 mg L-1, the highest reported so far. Mass spectrometry analysis showed a high glycosylation efficiency of the designed glycosylation sequon in CTB-O5 and CTB-O7, demonstrating the effectiveness of the glyco-optimized platform in producing vaccines against ExPEC.
CARBOHYDRATE POLYMERS
(2021)
Article
Immunology
Shujie Li, Yu Pang, Si Zhang, Qing Li, Qian Wang, Lei Wang, Lu Feng
Summary: This study provides the first comprehensive analysis of the transcriptome profile of UPEC during its intra-BECs life, revealing a new virulence-associated gene and its regulatory mechanism.
MICROBIAL PATHOGENESIS
(2021)
Article
Multidisciplinary Sciences
Lingyan Jiang, Peisheng Wang, Xiaorui Song, Huan Zhang, Shuangshuang Ma, Jingting Wang, Wanwu Li, Runxia Lv, Xiaoqian Liu, Shuai Ma, Jiaqi Yan, Haiyan Zhou, Di Huang, Zhihui Cheng, Chen Yang, Lu Feng, Lei Wang
Summary: S. Typhimurium infection induces upregulated glycolysis and decreased serine synthesis in host macrophages, leading to accumulation of glycolytic intermediates that promote bacterial replication and virulence.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Yutao Liu, Bin Liu, Tingting Xu, Qian Wang, Wendi Li, Jialin Wu, Xiaoyu Zheng, Bin Liu, Ruiying Liu, Xingmei Liu, Xi Guo, Lu Feng, Lei Wang
Summary: The study identifies a fructose uptake system crucial for the growth of Vibrio cholerae in seawater, where fructose concentrations are low. This system may be important for the survival of pandemic V. cholerae in seawater.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Applied
Xiaolong Jiang, Jing Bai, Huijing Zhang, Jian Yuan, Gege Lu, Yuhui Wang, Lingyan Jiang, Bin Liu, Di Huang, Lu Feng
Summary: ExPEC O1 is a common pathogen causing serious infections and often resistant to antibiotics. A glycoconjugate vaccine was designed to target this strain, with promising results in producing OPS antigen and bioconjugates in a glyco-optimized chassis strain, leading to successful generation of specific IgG antibodies and protection in mice against ExPEC O1 challenge. This plug-and-play glyco-optimized platform shows potential for future medical applications.
CARBOHYDRATE POLYMERS
(2022)
Article
Microbiology
Bin Liu, Chengqian Qian, Pan Wu, Xiaodan Li, Yutao Liu, Huiqian Mu, Min Huang, Yang Zhang, Tianyuan Jia, Yuanyuan Wang, Lu Wang, Xiao Zhang, Di Huang, Bin Yang, Lu Feng, Lei Wang
Summary: Enterohemorrhagic Escherichia coli regulates its cell wall structure during infection, balancing survival in the intestinal lumen and infection of intestinal epithelial cells. The bacteria express long-chain OAg in the intestinal lumen for increased resistance to antimicrobial peptides, but reduce OAg chain length upon sensing epithelial cell attachment, leading to enhanced injection of T3SS effectors into epithelial cells for host cell infection. This regulatory mechanism may be widespread in pathogenic bacteria and closely related to bacterial pathogenesis.
Article
Food Science & Technology
Min Wang, Bin Liu, Yanli Xu, Xi Guo, Tian Fu, Jiafei Liu, Lei Wang
Summary: In this study, the O-antigen gene clusters of V. fluvialis were analyzed by whole-genome sequencing, and a microsphere-based suspension array (MSA) was developed for the identification of different serogroups. Additionally, 13 new O-antigen gene cluster types were discovered. This method provides a rapid and efficient tool for detecting and identifying V. fluvialis serogroups, with potential applications in epidemiological investigations and food safety.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Cell Biology
Yu Pang, Zhihui Cheng, Si Zhang, Shujie Li, Xueping Li, Xiaodan Li, Xiao Zhang, Xiaoxiao Li, Yingxing Feng, Heting Cui, Zhen Chen, Le Liu, Qing Li, Jianxiao Huang, Mingqing Zhang, Siwei Zhu, Lei Wang, Lu Feng
Summary: Urinary tract infections are mainly caused by uropathogenic Escherichia coli (UPEC). This study reveals that UPEC senses host immune responses and initiates escape by upregulating a key phospholipase. The findings provide insights into pathogenic bacteria's response to intracellular phosphate shortage and tackling host defense, and offer potential for developing new therapeutic agents to treat UPEC infection.
Article
Cell Biology
Lu Feng, Bin Yang, Yongchang Xu, Yingying Xiong, Fang Wang, Bin Liu, Wen Yang, Ting Yao, Lei Wang
Summary: This study reveals a signal transduction pathway for the activation of virulence in EHEC O157:H7, providing insights into the relationship between adherence, gene regulation, and pathogenicity.
Article
Cell Biology
Hao Sun, Xuehua Wan, Yu Fan, Peng Liu, Yajun Song, Ningyu Zhu, Zhifeng Duan, Qian Wang, Fang Chen, Changhong Zhou, Yangyang Zheng, Peng Ding, Fenxia Liu, Lu Feng, Kwang Sik Kim, Lei Wang
Summary: The immune response of brain cells to invading bacteria and the mechanism used by pathogenic bacteria to escape immune surveillance in the brain are still not well understood. Microglia can engulf pathogenic bacteria and activate astrocytes to produce C3 for antibacterial activity. Pathogenic bacteria can sense low concentration of threonine in cerebrospinal fluid to reduce the immune response.
Article
Multidisciplinary Sciences
Bin Liu, Yutao Liu, Bin Yang, Qian Wang, Xingmei Liu, Jingliang Qin, Ke Zhao, Fan Li, Xiaohui Feng, Linxing Li, Pan Wu, Miaomiao Liu, Siwei Zhu, Lu Feng, Lei Wang
Summary: This study reveals that EHEC O157:H7 acquired a previously uncharacterized regulatory system rbfSR, which senses riboflavin produced by gut microbiota and activates the expression of virulence genes. This acquisition represents an essential step in the evolution of the highly pathogenic O157:H7. The introduction of rbfSR into other EHEC serotypes also increased their pathogenicity, indicating a potential public health issue.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Food Science & Technology
Guangfei Wei, Yichuan Liang, Guozhuang Zhang, Zhaoyu Zhang, Yongqing Zhang, Shilin Chen, Linlin Dong
Summary: This study investigated the presence of fungi on the surface of Polygonum multiflorum Thunb and found that it is easily contaminated by toxigenic fungi and mycotoxins, posing a health risk for consumers. The results also showed that sampling location and processing can influence the assembly and network complexity of the surface microbiome of Polygonum multiflorum Thunb.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Gabriele Busetta, Giuliana Garofalo, Salvatore Claps, Maria Teresa Sardina, Elena Franciosi, Antonio Alfonzo, Nicola Francesca, Giancarlo Moschetti, Luca Settanni, Raimondo Gaglio
Summary: This study evaluates the microbial diversity of wooden shelves used for the ripening of PDO Pecorino di Filiano and PGI Canestrato di Moliterno cheeses. The results show a strict correlation between the microbiota of wooden shelves and cheese rinds, indicating the positive role of wooden shelves in the cheese ripening process.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Massimo Todaro, Giuliana Garofalo, Gabriele Busetta, Riccardo Gannuscio, Ambra Rita Di Rosa, Maria Luisa Scatassa, Cinzia Cardamone, Isabella Mancuso, Elena Franciosi, Fatima Rando, Monica Agnolucci, Vincenzo Chiofalo, Raimondo Gaglio, Luca Settanni
Summary: This study aimed to reduce the production time of PDO Pecorino Siciliano cheese by comparing two cooking methods and assessing their impact on microbiological composition and lactic acid bacteria populations. The results showed that the different cooking methods did not affect the physicochemical characteristics of the cheese, and sensory evaluation confirmed that the modified production protocol did not significantly alter the product characteristics and overall acceptance. Therefore, cooking under hot water proved to be an effective way to reduce the transformation duration while maintaining the typicality of PDO Pecorino Siciliano cheese.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Yingying Sun, Yana Liu, Weiwei Zhou, Lele Shao, Han Wang, Yijie Zhao, Bo Zou, Xingmin Li, Ruitong Dai
Summary: The study evaluates the effect of Ohmic heating (OH) at various voltage gradients and water bath on microbial inactivation, physicochemical and sensory properties, and microbial flora of pasteurized milk. Results show that OH with higher voltage effectively inactivates microorganisms, slows down lipid oxidation, and maintains sensory quality and amino acids content of milk. The microbial community in milk treated with OH remains relatively stable during storage.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Minjin Oh, Ana Cevallos-Urena, Byoung Sik Kim
Summary: In this study, two bacteriophages, PECP14 and PECP20, infecting Escherichia coli O157:H7 were isolated and found to be stable under different environmental conditions. These phages exhibited specificity in targeting different host receptors and demonstrated the ability to infect other foodborne pathogens. The efficacy of phage treatment was demonstrated through rapid adsorption and bacterial lysis. Furthermore, purified endolysins derived from these phages showed remarkable bacteriolytic activity not only against E. coli O157:H7 but also against other pathogens. These findings suggest that these phages and their endolysins can be used for biological control of E. coli O157:H7 in various stages of agricultural product production.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Review
Food Science & Technology
Filippo Marzoli, Michela Bertola, Juliane Pinarelli Fazion, Giulia Cento, Pietro Antonelli, Beatrice Dolzan, Lisa Barco, Simone Belluco
Summary: Insects are a sustainable and protein-rich food source, and Salmonella is a common pathogen in insects. This systematic review aims to collect and evaluate existing studies on the occurrence of Salmonella in crickets and mealworms. Although the number of studies is limited and there are some limitations, this review highlights the importance of obtaining reliable data on the presence of Salmonella in insects, considering the growing market and increased farming scale.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Review
Food Science & Technology
Lucas Chikhi, Melanie Mancier, Hubert Brugere, Bertrand Lombard, Lyazrhi Faouzi, Laurent Guillier, Nathalie Gnanou Besse
Summary: Listeriosis is a severe foodborne disease with a high mortality rate, mainly caused by L. monocytogenes. The EU regulation sets qualitative or quantitative food safety criteria for L. monocytogenes and recommends the use of EN ISO 11290-1 as the reference detection method. Our study compares alternative certified methods for detecting L. monocytogenes, highlighting the importance of diversifying available detection methods.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Christina A. M. DeWitt, Kevin A. Nelson, Hyung Joo Kim, David H. Kingsley
Summary: High pressure processing (HPP) is a non-thermal method that can effectively inactivate pathogens. Research has shown that applying high pressure to thawed samples in an ultra-low temperature environment enhances the inactivation of norovirus.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Suzane Manzini, Thaina Valente Bertozzo, Isabella Neves Aires, Nassarah Jabur Lot Rodrigues, Amanda Bezerra Bertolini, Marcela Alexandrino, Jackieline Sampaio Steinle, Renata Pimentel Bandeira de Melo, Rinaldo Aparecido Mota, Maria Izabel Merino de Medeiros, Virginia Bodelao Richini-Pereira, Vera Claudia Lorenzetti Magalhaes Curci, Simone Baldini Lucheis
Summary: This study detected Toxoplasma gondii DNA in raw bovine milk samples from expansion tanks in small properties located in different cities of the Midwest region of Sa similar to o Paulo, Brazil using PCR. The results showed that the TgNP1 and TgNP2 genes were more efficient in detecting T. gondii DNA, highlighting the importance of raw bovine milk as a potential source of human infections caused by this parasite, and emphasizing the need for proper pasteurization and further understanding of the epidemiology of this protozoan.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Luca Bolzoni, Mauro Conter, Luca Lamperti, Erika Scaltriti, Marina Morganti, Antonio Poeta, Marco Vecchi, Silvia Paglioli, Alessandra Rampini, Paolo Ramoni, Daniela De Vita, Cristina Bacci, Martina Rega, Laura Andriani, Stefano Pongolini, Silvia Bonardi
Summary: This study assessed the role of equids as carriers of Salmonella and the occurrence of contaminated equid carcasses during the slaughter process. Salmonella was detected in a small percentage of equids' caecal contents, but not in carcass samples. Additionally, a genetic match was found between Salmonella strains from horse caecal contents and human cases, suggesting a link between horse meat consumption and salmonellosis.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Daria Nowinski, Tomasz Czapka, Irena Maliszewska
Summary: The effect of multiple sublethal doses of non-thermal plasma treatments on phenotypical changes and the reduction in phytopathogenicity of three species of fungi was investigated. The plasma damaged the surface of the fungal hyphae, decreased their dry biomass, and affected the activity of pectinase and xylanase. Furthermore, the plasma treatments increased the germination rate of artificially infected cucumber seeds and resulted in plants with higher biomass and longer roots and stems. However, the reduction in phytopathogenicity was not complete after the multiple plasma treatments.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)