Article
Multidisciplinary Sciences
Aenne Harberts, Constantin Schmidt, Joanna Schmid, Daniel Reimers, Friedrich Koch-Nolte, Hans-Willi Mittruecker, Friederike Raczkowski
Summary: IRF4 is essential for the activation and reactivation of CD8(+) memory T cells, but does not affect their overall survival. However, the formation and maintenance of CD8(+) tissue-resident memory T cells appears to depend on IRF4.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Joseph S. Dolina, Joey Lee, Eugene L. Moore, Jennifer L. Hope, Donald T. Gracias, Takaji Matsutani, Ashu Chawla, Jason A. Greenbaum, Joel Linden, Stephen P. Schoenberger
Summary: During acute infection, CD4(+) regulatory T cells play a crucial role in regulating the immune response by suppressing cytotoxic CD8(+) T lymphocytes through different mechanisms. Early T cells inhibit primary CTL expansion through adenosine production, while late-phase T-regs suppress T cell proliferation through the transfer of cAMP. This study reveals the distinct roles of different T-reg lineages in fine-tuning CTL priming and contraction phases during acute infection.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemical Research Methods
Ryan D. Sheldon, Eric H. Ma, Lisa M. DeCamp, Kelsey S. Williams, Russell G. Jones
Summary: The protocol integrates traditional immunology infection models with whole-body metabolite infusion and mass-spectrometry-based metabolomic profiling to evaluate T-cell metabolism. Researchers can assess the metabolic substrate utilization of specific T-cell subpopulations in vivo using this method.
Article
Biochemistry & Molecular Biology
Yi Feng, Shu Yao, Shan Li, Zuxiang Peng, Guoying Feng, Yan Ma, Bo Guo, Hongming Liu
Summary: Homozygous mutations in the AIRE gene lead to APECED by impairing thymic negative selection of autoreactive T cells. Moreover, the study reveals the crucial role of extrathymic Aire-expressing cells in shaping or sustaining memory T cells. These findings provide important insights into the T-cell response to infection.
Article
Cell Biology
Sae-Hae Kim, Byeol-Hee Cho, Kwang Soon Kim, Yong-Suk Jang
Summary: C5a in the Peyer's patch enhances CD8(+) T cell responses by increasing C5/C5a levels. Single-cell RNA sequencing identifies dendritic cells expressing C5aR and lysozyme as key players in CD8(+) T cell immune induction.
Article
Immunology
Zeyu Wang, Ziyu Dai, Hao Zhang, Xisong Liang, Xun Zhang, Zhipeng Wen, Peng Luo, Jian Zhang, Zaoqu Liu, Mingyu Zhang, Quan Cheng
Summary: In this study, lactate was found to contribute to the immune suppressive microenvironment in glioblastoma through RNA sequencing and machine learning algorithms. High levels of lactate can affect the migration and infiltration of CD8 T cells in glioblastoma.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Lang Jiang, Lu Liu, Miaomiao Zhang, Linxia Zhang, Cuisong Zhu, Qian He, Lilin Ye, Chen Zhao, Zejun Li, Jianqing Xu, Xiaoyan Zhang
Summary: Research has shown that CD8+ lung tissue resident memory T (T-RM) cells play a crucial role in protecting against respiratory viruses. By studying mouse infection models, it was found that lung CD8+ T-RM cells can quickly respond to antigen re-exposure and induce interferon-stimulated genes (ISGs) in the surrounding tissues. The study also revealed that the induction of certain important antiviral ISGs and the suppression of key inflammatory cytokines are dependent on IFN-gamma signaling.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Snehanshu Chowdhury, Anwesha Kar, Debaleena Bhowmik, Anupam Gautam, Debashree Basak, Ishita Sarkar, Puspendu Ghosh, Deborpita Sarkar, Alvina Deka, Paramita Chakraborty, Asima Mukhopadhyay, Shikhar Mehrotra, Soumen Basak, Sandip Paul, Shilpak Chatterjee
Summary: IL-12 stimulation increases intracellular acetyl CoA levels in CD8(+) T cells, allowing them to maintain effector function in nutrient-deprived tumor microenvironments. Inhibition of ATP-citrate lyase, an enzyme involved in the glucose-citrate-acetyl CoA pathway, impairs IFN gamma production and viability of CD8(+) T cells in nutrient-restricted conditions. CD8(+) T cells cultured in high pyruvate-containing media acquire metabolic features of IL-12-stimulated CD8(+) T cells and display improved antitumor potential.
Article
Gastroenterology & Hepatology
Yanan Wang, Yuan Hu, Yuxia Liu, Chongdeng Shi, Linyan Yu, Nan Lu, Cai Zhang
Summary: Liver-resident CD44(hi)CD27(-) gamma delta T cells have enhanced proliferation capacity and rapidly increase in proportion after infection. These cells can produce both IL-17A and IFN-gamma simultaneously and have a protective role against Listeria monocytogenes infection. Hepatic macrophages promote the migration and accumulation of these cells at infection sites.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Janine van Duijn, Maaike J. M. de Jong, Naomi Benne, Romain J. T. Leboux, Marieke E. van Ooijen, Nicky Kruit, Amanda C. Foks, Wim Jiskoot, Ilze Bot, Johan Kuiper, Bram Slutter
Summary: In an atherosclerotic mouse model, a lesion-localized increase in Tc17 cells was found. Tc17 cells appeared to be non-atherogenic compared to their Tc0 counterparts.
CARDIOVASCULAR RESEARCH
(2021)
Article
Immunology
Diego von Werdt, Bilgi Gungor, Juliana Barreto de Albuquerque, Thomas Gruber, Daniel Zysset, Cheong K. C. Kwong Chung, Antonia Correa-Ferreira, Regina Berchtold, Nicolas Page, Mirjam Schenk, John H. Kehrl, Doron Merkler, Beat A. Imhof, Jens V. Stein, Jun Abe, Gleb Turchinovich, Daniela Finke, Adrian C. Hayday, Nadia Corazza, Christoph Mueller
Summary: Members of the Regulator of G-protein signaling (Rgs) family regulate G protein signaling by increasing the GTPase activity of G alpha protein subunits. Rgs1, a member of the Rgs family, preferentially deactivates G alpha q and G alpha i subunits, attenuating chemokine receptor-mediated immune cell trafficking. Its impact on tissue-resident T cells and immunosurveillance in barrier tissues is not fully understood.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Marie Boutet, Zachary Benet, Erik Guillen, Caroline Koch, Saidi M'Homa Soudja, Fabien Delahaye, David Fooksman, Gregoire Lauvau
Summary: Research shows that rapid and effective CD8(+) T cell responses require spatially and temporally coordinated events, delivering activating chemokines locally to CCR2(+)Ly6C(+) monocytes to quickly restrict microbial pathogen growth. CD8(+) T cells recognize cognate antigen on dendritic cells, initiating a burst of chemokines production and delivering IFN gamma and chemokines to CCR2(+)Ly6C(+) monocytes for microbicidal activities.
Article
Food Science & Technology
Ying Feng, Zhangkai Cheng, Xianhu Wei, Moutong Chen, Jumei Zhang, Youxiong Zhang, Liang Xue, Minling Chen, Fan Li, Yuting Shang, Tingting Liang, Yu Ding, Qingping Wu
Summary: In this study, a novel method that combines metabolomics with deep learning was developed for the detection and identification of Listeria monocytogenes. By establishing a convolutional neural network model and using a pseudotargeted metabolomics approach, high prediction accuracies were achieved and different Listeria species were successfully distinguished. This is a promising powerful tool for the detection and classification of pathogens.
Article
Immunology
Juliana Barreto de Albuquerque, Lukas M. Altenburger, Jun Abe, Diego von Werdt, Stefanie Wissmann, Jose Martinez Magdaleno, David Francisco, Geert van Geest, Xenia Ficht, Matteo Iannacone, Remy Bruggmann, Christoph Mueller, Jens Stein
Summary: The study identifies mandibular lymph nodes as sentinel lymphoid organs in intercepting ingested Listeria monocytogenes. Oral uptake of Lm leads to the activation and release of antigen-specific CD8(+) T cells, contributing to rapid clearance of the pathogen. Interestingly, CD8(+) T-EFF generated in the upper GI tract lack gut-seeking phenotype compared to those generated in the lower GI tract.
SCIENCE IMMUNOLOGY
(2022)
Article
Immunology
Woojong Lee, Autumn Larsen, Brock Kingstad-Bakke, Chandranaik B. Marinaik, M. Suresh
Summary: The nanoemulsion adjuvant Adjuplex (ADJ) enhances immunity to L. monocytogenes by promoting CD8 T cell response, especially when combined with Toll-like receptor agonists. ADJ effectively activates specific DCs to induce effector CD8 T cells, providing protective immunity against L. monocytogenes.
INFECTION AND IMMUNITY
(2021)
Article
Immunology
Grant S. Jones, Kate M. Bussell, Tanya Myers-Morales, Abigail M. Fieldhouse, Elsa N. Bou Ghanem, Sarah E. F. D'Orazio
INFECTION AND IMMUNITY
(2015)
Article
Immunology
Michelle G. Pitts, Tanya Myers-Morales, Sarah E. F. D'Orazio
JOURNAL OF IMMUNOLOGY
(2016)
Article
Immunology
Grant S. Jones, Victoria C. Smith, Sarah E. F. D'Orazio
JOURNAL OF IMMUNOLOGY
(2017)
Article
Immunology
Grant S. Jones, Sarah E. F. D'Orazio
JOURNAL OF IMMUNOLOGY
(2017)
Article
Immunology
Michelle G. Pitts, Travis A. Combs, Sarah E. F. D'Orazio
INFECTION AND IMMUNITY
(2018)
Article
Multidisciplinary Sciences
Jakob Schardt, Grant Jones, Stefanie Mueller-Herbst, Kristina Schauer, Sarah E. F. D'Orazio, Thilo M. Fuchs
SCIENTIFIC REPORTS
(2017)
Editorial Material
Microbiology
Michael J. Imperiale, Ira Blader, Patricia Bradford, Sarah D'Orazio, W. Paul Duprex, Craig D. Ellermeier, Ana Fernandez-Sesma, Katherine McMahon, Aaron Mitchell, Marcela F. Pasetti, Susannah Tringe
Article
Immunology
Michelle G. Pitts, Sarah E. F. D'Orazio
JOURNAL OF IMMUNOLOGY
(2019)
Editorial Material
Microbiology
Aaron Mitchell, Ira Blader, Patricia Bradford, Sarah D'Orazio, W. Paul Duprex, Craig D. Ellermeier, Ana Fernandez-Sesma, Michael J. Imperiale, Katherine McMahon, Marcela F. Pasetti, Susannah Tringe
Article
Microbiology
Sarah E. F. D'Orazio
MICROBIOLOGY SPECTRUM
(2019)
Article
Multidisciplinary Sciences
Samuel H. Light, Raphael Meheust, Jessica L. Ferrell, Jooyoung Cho, David Deng, Marco Agostoni, Anthony T. Iavarone, Jillian F. Banfield, Sarah E. F. D'Orazio, Daniel A. Portnoy
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Microbiology
Taylor E. Senay, Jessica L. Ferrell, Filip G. Garrett, Taylor M. Albrecht, Jooyoung Cho, Katie L. Alexander, Tanya Myers-Morales, Olivia F. Grothaus, Sarah E. F. D'Orazio
Article
Immunology
Jamila S. Tucker, Jooyoung Cho, Taylor M. Albrecht, Jessica L. Ferrell, Sarah E. F. D'Orazio
Summary: Through research, it was found that the facultative intracellular foodborne pathogen Listeria monocytogenes can replicate inside the mesenteric lymph nodes (MLN) draining the small intestine and further spread via ActA-mediated cell-to-cell transmission. Replication in the MLN draining the colon is not necessary, but rather serves to amplify bacterial burdens for more efficient colonization.
INFECTION AND IMMUNITY
(2023)
Article
Microbiology
Taylor M. Albrecht, Zuzana Kucerova, Sarah E. F. D'Orazio
Summary: The study presents the whole-genome sequences of two neurotropic lineage III strains of Listeria monocytogenes, UKVDL9 isolated from a sheep brain and 2010L-2198 isolated from a human subject with rhombencephalitis, with an edited draft genome sequence for 2010L-2198.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Review
Microbiology
Michelle G. Pitts, Sarah E. F. D'Orazio