Article
Biology
Ruchi Roy, Janet Zayas, Sunil K. Singh, Kaylee Delgado, Stephen J. Wood, Mohamed F. Mohamed, Dulce M. Frausto, Yasmeen A. Albalawi, Thea P. Price, Ricardo Estupinian, Eileena F. Giurini, Timothy M. Kuzel, Andrew Zloza, Jochen Reiser, Sasha H. Shafikhani
Summary: Infection is a common complication that hinders the healing process in diabetic wounds. Research has found that exposure to glucose impairs chemotaxis signaling in diabetic neutrophils, leading to reduced chemotaxis and delayed migration, making diabetic wounds more susceptible to infection. However, the pro-inflammatory cytokine CCL3 can override the impaired signaling and improve infection control, as well as stimulate healing in diabetic wounds.
Article
Food Science & Technology
Kierra S. Hardy, Maxx H. Tessmer, Dara W. Frank, Jonathon P. Audia
Summary: Pseudomonas aeruginosa is an opportunistic, Gram-negative pathogen known to cause hospital acquired infections in immunocompromised patients. Highly virulent strains of P. aeruginosa use a type III secretion system to inject exoenzyme effectors directly into the cytoplasm of a target host cell, leading to adverse outcomes. The T3SS effector ExoU is a highly cytotoxic phospholipase A(2) enzyme that damages host cell membranes and subverts the innate immune response to infection.
Article
Immunology
Haofeng Xu, Lili Huang, Qin Luo, Qianqian Tu, Jiayu Liu, Renlin Yu, Jun Huang, Te Chen, Yibing Yin, Ju Cao
Summary: Research shows that absence of TLR7 provides protective effects during Pseudomonas aeruginosa pneumonia, improving survival and bacterial clearance while reducing inflammation and lung pathology.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ke-Qin Gong, Carmen Mikacenic, Matthew E. Long, Charles W. Frevert, Timothy P. Birkland, Jean Charron, Sina A. Gharib, Anne M. Manicone
Summary: Inhibition of MAP2K2 can improve acute lung injury (ALI) and deficiency of MAP2K2 can accelerate the resolution of ALI. Genetic variants in MAP2K2 are associated with mortality from acute respiratory distress syndrome (ARDS). MAP2K2 plays an important role in promoting and sustaining proinflammatory pathway activation in ALI.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Microbiology
Xu Yang, Anwarul Haque, Shigenobu Matsuzaki, Tetsuya Matsumoto, Shigeki Nakamura
Summary: The study investigated the use of bacteriophage KPP10 for treating Pseudomonas aeruginosa-induced pneumonia in mouse models. Results showed that phage treatment significantly improved survival rate, reduced bacterial count and inflammation, suggesting the potential of phage therapy for P. aeruginosa lung infections.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Adrienne R. Kambouris, Jerod A. Brammer, Holly Roussey, Chixiang Chen, Alan S. Cross
Summary: The combination of burn state and infection affects gene expression in both the host and pathogen, increasing the propensity for infection. Inhibiting IL-10 signaling or co-administering arginine can improve survival rates in a burn and infection model.
Article
Immunology
Bianca L. Ferreira, Ivan Ramirez-Moral, Natasja A. Otto, Reinaldo Salomao, Alex F. de Vos, Tom van der Poll
Summary: During acute P. aeruginosa pneumonia, the PPAR-gamma agonist pioglitazone exerts a selective proinflammatory effect on bronchial epithelial cells, possibly by enhancing intracellular glycolysis.
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2022)
Article
Microbiology
Nitasha D. Menon, Samuel Penziner, Elizabeth T. Montano, Raymond Zurich, David T. Pride, Bipin G. Nair, Geetha B. Kumar, Victor Nizet
Summary: Bacteriophage therapy is an alternative treatment to antibiotics for multidrug-resistant pathogens. This study found that phage therapy can lead to the emergence of phage-resistant mutants with pyomelanin pigmentation, but these mutants are less virulent due to large genomic deletions and retain susceptibility to the antibiotic colistin. This suggests that they do not pose a contraindication to using anti-pseudomonal phage therapy.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Review
Immunology
Stephane Pont, Anne-Beatrice Blanc-Potard
Summary: Pseudomonas aeruginosa is an opportunistic human pathogen responsible for acute infections and a major cause of mortality in CF patients. The zebrafish embryo model is a powerful tool to study P. aeruginosa pathogenesis and host immune response, especially in a CF context.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biology
See-Yeun Ting, Kaitlyn D. LaCourse, Hannah E. Ledvina, Rutan Zhang, Matthew C. Radey, Hemantha D. Kulasekara, Rahul Somavanshi, Savannah K. Bertolli, Larry A. Gallagher, Jennifer Kim, Kelsi M. Penewit, Stephen J. Salipante, Libin Xu, S. Brook Peterson, Joseph D. Mougous
Summary: Pseudomonas aeruginosa shows a coordinated defense against bacterial threats by activating conserved interbacterial antagonism resistance clusters, similar to eukaryotic innate immunity.
Article
Biochemistry & Molecular Biology
Tooba Sadat Ahmadi, Seyed Latif Mousavi Gargari, Daryush Talei
Summary: The study evaluated the antibacterial potency of IgY antibodies raised against Pseudomonas aeruginosa flagellin, confirming their role in reducing bacterial motility, biofilm formation, and invasion ability, as well as providing high protective efficacy through polymorphonuclear leukocyte-enhanced bacterial killing. The protective efficacy of anti-flagellin IgY antibodies was demonstrated in animal models, highlighting their potential as a novel therapeutic option against PA infection.
MOLECULAR IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Ehsan Zare Banadkoki, Iraj Rasooli, Tooba Ghazanfari, Seyed Davar Siadat, Mehdi Shafiee Ardestani, Parviz Owlia
Summary: This study evaluated the immunogenicity and efficacy of a vaccine consisting of Pseudomonas aeruginosa PA-OMVs (PA-OMVs) conjugated with diphtheria toxoid (DT) in a mice model of burn wound infection. The results showed that the conjugated vaccine significantly increased specific antibodies titer and provided greater protective effectiveness against P. aeruginosa infection, as seen by lower bacterial loads, decreased inflammatory cell infiltration, and less tissue damage.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Jiarong Zhou, Nishta Krishnan, Zhongyuan Guo, Christian J. Ventura, Maya Holay, Qiangzhe Zhang, Xiaoli Wei, Weiwei Gao, Ronnie H. Fang, Liangfang Zhang
Summary: This study investigates the effectiveness of a biomimetic nanotoxoid vaccine in protecting immunodeficient animals from Pseudomonas aeruginosa infection. The nanotoxoids utilize a macrophage membrane coating to sequester and present bacterial virulence factors, providing rapid and long-lasting immunity. The nanovaccine can be administered through multiple routes and effectively protects against lethal infections in pneumonia and septicemia models.
Article
Infectious Diseases
Gamal El-Nobi, Mohammed Hassanin, Alshimaa A. Khalil, Alaa Y. Mohammed, Shimaa A. Amer, Metwally M. Montaser, Mohamed E. El-sharnouby
Summary: The study found that synbiotic additives significantly improved immune parameters and enhanced antioxidant activity in O. niloticus, increasing the resistance against P. aeruginosa infection.
Article
Microbiology
Fabio Aguiar-Alves, Hoan N. Le, Vuvi G. Tran, Emmanuelle Gras, Trang T. T. Vu, Oliver X. Dong, Josiane Silva Quetz, Lily Cheng, Li Yu, Bret R. Sellman, Charles K. Stover, Antonio DiGiandomenico, Binh An Diep
Summary: The use of MEDI3902 antibody for prophylactic treatment has been shown to protect rabbits from Pseudomonas aeruginosa infection and reduce lung lesions and bacteremia. Compared to MEDI3902 treatment, rabbits treated with irrelevant control IgG exhibited severe pneumonia and systemic pathophysiological changes. These results suggest that MEDI3902 prophylaxis can decrease the severity of P. aeruginosa ventilator-associated pneumonia.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Immunology
Stephen J. Gurczynski, Niket Nathani, Helen I. Warheit-Niemi, Elissa M. Hult, Amy Podsiad, Jane Deng, Rachel L. Zemans, Urvashi Bhan, Bethany B. Moore
MUCOSAL IMMUNOLOGY
(2019)
Article
Physiology
Lei Sun, Elissa M. Hult, Timothy T. Cornell, Kevin K. Kim, Thomas P. Shanley, Carol A. Wilke, Manisha Agarwal, Stephen J. Gurczynski, Bethany B. Moore, Mary K. Dahmer
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2019)
Article
Critical Care Medicine
David N. O'Dwyer, Shanna L. Ashley, Stephen J. Gurczynski, Meng Xia, Carol Wilke, Nicole R. Falkowski, Katy C. Norman, Kelly B. Arnold, Gary B. Huffnagle, Margaret L. Salisbury, MeiLan K. Han, Kevin R. Flaherty, Eric S. White, Fernando J. Martinez, John R. Erb-Downward, Susan Murray, Bethany B. Moore, Robert P. Dickson
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2019)
Article
Critical Care Medicine
Xiaofeng Zhou, David N. O'Dwyer, Meng Xia, Holly K. Miller, Paul R. Chan, Kelsey Trulik, Mathew M. Chadwick, Timothy C. Hoffman, Camille Bulte, Kevin Sekerak, Carol A. Wilke, Swapneel J. Patel, Wayne M. Yokoyama, Susan Murray, Gregory A. Yanik, Bethany B. Moore
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2019)
Article
Biochemistry & Molecular Biology
Daniel J. Schneider, Katherine A. Smith, Catrina Latuszek, Carol A. Wilke, Danny M. Lyons, Loka R. Penke, Jennifer M. Speth, Matangi Marthi, Joel A. Swanson, Bethany B. Moore, Adam S. Lauring, Marc Peters-Golden
Editorial Material
Immunology
Stephen J. Gurczynski, Bethany B. Moore
MUCOSAL IMMUNOLOGY
(2020)
Article
Multidisciplinary Sciences
Eiji Saito, Stephen J. Gurczynski, Kevin R. Kramer, Carol A. Wilke, Stephen D. Miller, Bethany B. Moore, Lonnie D. Shea
Article
Medicine, Research & Experimental
Stephen J. Gurczynski, Nicolas L. Pereira, Steven M. Hrycaj, Carol Wilke, Rachel L. Zemans, Bethany B. Moore
Summary: The aryl-hydrocarbon receptor (AHR) plays a key role in controlling IL-17 responses and pulmonary fibrosis following bone marrow transplant (BMT) by regulating the production of AHR ligands in response to respiratory pathogens. Inhibition or genetic ablation of AHR signaling results in decreased IL-17 expression and lung pathology, indicating the importance of the TDO2/AHR axis in shaping immune responses to respiratory pathogens.
Article
Physiology
Elissa M. Hult, Stephen J. Gurczynski, Bethany B. Moore
Summary: Macrophages play a critical role in pulmonary fibrosis, with M2 macrophages being associated with profibrotic effects. Research shows that M2 macrophage conditioned media can promote fibroblast migration and proliferation, alveolar epithelial cell apoptosis, and the expression of fibrotic genes. The profibrotic effects of M2 macrophage conditioned media are mainly attributed to the polarization cytokines IL-4 and IL-13.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Physiology
Scott J. Denstaedt, Angela C. Bustamante, Michael W. Newstead, Bethany B. Moore, Theodore J. Standiford, Rachel L. Zemans, Benjamin H. Singer
Summary: Millions of sepsis survivors suffer from late pulmonary complications, possibly due to persistent immune reprogramming. This study used a murine sepsis model to show enhanced/primed immune responses in the lungs and identified S100A8/A9 as a potential biomarker and therapeutic target for long-term pulmonary complications. This provides insight into the mechanisms mediating enhanced immune responses in sepsis survivors and paves the way for further investigation.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Physiology
Jay H. Lipinski, Nicole R. Falkowski, Gary B. Huffnagle, John R. Erb-Downward, Robert P. Dickson, Beth B. Moore, David N. O'Dwyer
Summary: The absence of toll-like receptors results in altered lung microbiota with changes in community composition, reduced diversity, and bacterial burden. Co-housing wild-type mice with toll-like receptor-deficient mice for 3 weeks did not significantly affect the composition of the lung microbiome. Toll-like receptor signaling plays a role in shaping lung microbiota.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Medicine, Research & Experimental
Helen Warheit-Niemi, Summer J. Edwards, Shuvasree SenGupta, Carole A. Parent, Xiaofeng Zhou, David N. O'Dwyer, Bethany B. Moore
Summary: This study found that fibrotic mice infected with MRSA showed increased morbidity and mortality compared to nonfibrotic mice. Fibrosis led to a defect in MRSA clearance due to impaired innate immune responses, including neutrophil killing of MRSA and lung macrophage phagocytosis of MRSA.
Article
Biochemistry & Molecular Biology
Elissa M. Hult, Stephen J. Gurczynski, David N. O'Dwyer, Rachel L. Zemans, Andrew Rasky, Yizhuo Wang, Susan Murray, Howard C. Crawford, Bethany B. Moore
Summary: Idiopathic pulmonary fibrosis (IPF) is a progressive and lethal lung disease without known cure. Chronic inflammation and the involvement of HB-EGF have been observed in IPF. This study demonstrates that there is increased expression of HB-EGF in IPF patients and mice with pulmonary fibrosis, and HB-EGF plays a critical role in regulating fibrosis progression.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Respiratory System
Bethany B. Moore, Megan N. Ballinger, Natalie N. Bauer, Timothy S. Blackwell, Zea Borok, G. R. Scott Budinger, Blanca Camoretti-Mercado, Serpil C. Erzurum, Blanca E. Himes, Venkateshwar G. Keshamouni, Hrishikesh S. Kulkarni, Rama K. Mallampalli, Thomas J. Mariani, Fernando J. Martinez, Janet E. McCombs, Dawn C. Newcomb, Richard A. Johnston, Michael A. O'Reilly, Y. S. Prakash, Karen M. Ridge, Patricia J. Sime, Anne I. Sperling, Shelia Violette, David S. Wilkes, Melanie Konigshoff
Summary: The American Thoracic Society established an ad hoc committee to identify barriers and opportunities for non-clinically practicing basic and translational scientists to integrate into clinical units. Based on surveys of division chiefs and workshop participants, it was found that hiring Ph.D. scientists into clinical units often resulted in non-tenure track positions without the same support as basic science units. These barriers are primarily financial. The recommendation is for academic medical centers to consider strategies to integrate basic and translational scientists into clinical units in a meaningful way.
ANNALS OF THE AMERICAN THORACIC SOCIETY
(2023)
Article
Medicine, Research & Experimental
Carol A. Wilke, Mathew M. Chadwick, Paul R. Chan, Bethany B. Moore, Xiaofeng Zhou