Review
Biochemistry & Molecular Biology
Till Klecker, Benedikt Westermann
Summary: Mitochondria are complex organelles with two membranes, characterized by characteristic folds of the inner membrane called cristae. Recent studies have identified four major pathways that cooperate to shape cristae membranes, including ATP synthase dimer formation, assembly of MICOS, inner membrane remodelling by GTPase, and modulation of mitochondrial lipid composition. These pathways play a crucial role in establishing mitochondrial membrane architecture.
Article
Multidisciplinary Sciences
Christopher Jakubke, Rodaria Roussou, Andreas Maiser, Christina Schug, Felix Thoma, David Bunk, David Horl, Heinrich Leonhardt, Peter Walter, Till Klecker, Christof Osman
Summary: The study shows that yeast can distinguish between functional and defective mtDNA, promoting the generation of healthier daughter cells. Purifying selection for functional mtDNA occurs in a continuous mitochondrial network, depending on intact cristae morphology.
Article
Biochemical Research Methods
Magdalena Rzepka, Tamara Suhm, Martin Ott
Summary: This article presents a protocol for introducing reporter genes into the mitochondrial genome of budding yeast, allowing for investigation of mitochondrial gene expression. The technique is exemplified by the use of a mitochondrially encoded nanoluciferase to monitor mitochondrial translation levels under different growth conditions.
Article
Biochemistry & Molecular Biology
Masami Koushi, Rei Asakai
Summary: The study demonstrates that in Saccharomyces cerevisiae, the PTP formation is mediated by AACs instead of ATP synthase dimers, suggesting a role of AAC-associated pore regulated by porin1/2 and Cpr3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Gea Cereghetti, Caroline Wilson-Zbinden, Vera M. Kissling, Maren Diether, Alexandra Arm, Haneul Yoo, Ilaria Piazza, Shady Saad, Paola Picotti, D. Allan Drummond, Uwe Sauer, Reinhard Dechant, Matthias Peter
Summary: The glycolytic metabolite fructose-1,6-bisphosphate is able to initiate the disassembly of inactive amyloids formed by Cdc19 during stress, leading to the restoration of ATP production and disassembly of stress granules (SGs) in yeast cells. This mechanism directly links cellular metabolism with SG dynamics, and plays a critical role in stress recovery by promoting Cdc19 re-solubilization and enhancing ATP production.
NATURE CELL BIOLOGY
(2021)
Article
Food Science & Technology
Juan Antonio Porras-Aguera, Jaime Moreno-Garcia, Teresa Garcia-Martinez, Juan Moreno, Juan Carlos Mauricio
Summary: The prise de mousse stage during the elaboration of sparkling wine through the traditional method involves a second fermentation in a sealed bottle, which significantly contributes to the unique organoleptic properties of these wines. This study characterizes the mitochondrial response of Saccharomyces cerevisiae strains used in sparkling wine production and found that CO2 overpressure negatively affects mitochondrion-related proteins. However, proteins involved in wine aromas and respiratory chain are found to be over-represented in sealed bottles, with differences in concentrations observed depending on time and CO2 overpressure parameters. Ethanol content during fermentation may be a key factor in these proteomic changes, highlighting the importance of further research in this area.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Cell Biology
Heike Rampelt, Florian Wollweber, Mariya Licheva, Rinse de Boer, Inge Perschil, Liesa Steidle, Thomas Becker, Maria Bohnert, Ida van der Klei, Claudine Kraft, Martin van der Laan, Nikolaus Pfanner
Summary: Cristae, invaginations of the mitochondrial inner membrane, are important for oxidative phosphorylation. The study reveals that Mic10, a subunit of MICOS, also interacts with the F1Fo-ATP synthase, and it plays distinct roles in cristae shaping and respiratory adaptation and growth.
Review
Biochemistry & Molecular Biology
Alessandra Ferramosca, Vincenzo Zara
Summary: Yeast mitochondrial carriers play a crucial role in cellular metabolism by connecting mitochondrial and cytosolic reactions, contributing to various important metabolic pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Immunology
Yewei Yang, Wenbo Lei, Lanhua Zhao, Yating Wen, Zhongyu Li
Summary: Mitochondria play crucial roles in energy production, metabolism, apoptosis, and intrinsic immunity within cells. The infection of Chlamydia, an intracellular bacterium, is closely associated with the host cell mitochondria, affecting mitochondrial fusion and fission to create a favorable environment for Chlamydia replication and growth.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alexandr A. Tomashevsky, Valery V. Petrov
Summary: Both ATP and inorganic polyphosphates are involved in yeast energy homeostasis, with the PMA1 H+-ATPase playing a key role. The structure and phosphorylation of the enzyme affect its function and its relationship with PolyP metabolism. Substitutions of specific residues in different domains of the enzyme have varied effects on PolyP content and distribution, depending on the type of substitution and its impact on ATPase activity.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biochemistry & Molecular Biology
Emilia Baranowska, Katarzyna Niedzwiecka, Chiranjit Panja, Camille Charles, Alain Dautant, Jean-Paul di Rago, Deborah Tribouillard-Tanvier, Roza Kucharczyk
Summary: The mitochondrial DNA mutation m.9032T>C was found in NARP patients, causing maternal transmission and abnormalities in mitochondrial function. This mutation leads to a disruption in proton conduction between the external side of the membrane and the c-ring of ATP synthase. Intragenic suppressors were identified, restoring ATP synthase function to some extent.
HUMAN MOLECULAR GENETICS
(2023)
Article
Biotechnology & Applied Microbiology
Sha Xu, Guangjian Li, Jingwen Zhou, Guicai Chen, Jianzhong Shao
Summary: This study successfully engineered Saccharomyces cerevisiae for efficient anthocyanin production through metabolic engineering, providing new methods and approaches for anthocyanin production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anna Strizova, Paulina Smatralova, Petra Chovancikova, Zdenko Machala, Peter Polcic
Summary: Exposure of living cells to non-thermal plasma affects cell physiology and often results in cell death. This study investigated the involvement of cellular components or pathways in plasma-induced cell killing, revealing that mitochondria play a crucial role in both the damage and signaling, leading to cell protection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Takuya Matsumoto, Tomoki Osawa, Hikaru Taniguchi, Akira Saito, Ryosuke Yamada, Hiroyasu Ogino
Summary: This study focused on utilizing accumulated intermediates, including acetyl-CoA, in mitochondria to enhance carotenoid production in Saccharomyces cerevisiae. By localizing carotenoid-producing enzymes in the mitochondria, the strain showed improved carotenoid production compared to the strain with enzymes in the cytosol. Furthermore, increasing glucose concentration improved cell growth and carotenoid production in mitochondria, but not in the cytosol.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Cell Biology
Praveen K. Patnaik, Carine Beaupere, Hanna Barlit, Antonia Maria Romero, Mitsuhiro Tsuchiya, Michael Muir, Maria Teresa Martinez-Pastor, Sergi Puig, Matt Kaeberlein, Vyacheslav M. Labunskyy
Summary: This study reveals that defects in the mRNA-binding protein Cth2 contribute to iron dys-homeostasis, aging, and lifespan regulation in yeast. Modulation of Cth2 activity can impact mitochondrial function and extend lifespan.
Article
Biochemistry & Molecular Biology
Xin Su, Alain Dautant, Francois Godard, Marine Bouhier, Teresa Zoladek, Roza Kucharczyk, Jean-Paul di Rago, Deborah Tribouillard-Tanvier
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biology
Gaetan Bader, Ludovic Enkler, Yuhei Araiso, Marine Hemmerle, Krystyna Binko, Emilia Baranowska, Johan-Owen De Craene, Julie Ruer-Laventie, Jean Pieters, Deborah Tribouillard-Tanvier, Bruno Senger, Jean-Paul di Rago, Sylvie Friant, Roza Kucharczyk, Hubert Dominique Becker
Article
Cell Biology
Michela Carraro, Kristen Jones, Geppo Sartori, Marco Schiavone, Salvatore Antonucci, Roza Kucharczyk, Jean-Paul Di Rago, Cinzia Franchin, Giorgio Arrigoni, Michael Forte, Paolo Bernardi
Article
Biology
Qiuju Ding, Roza Kucharczyk, Weiwei Zhao, Alain Dautant, Shutian Xu, Katarzyna Niedzwiecka, Xin Su, Marie-France Giraud, Kewin Gombeau, Mingchao Zhang, Honglang Xie, Caihong Zeng, Marine Bouhier, Jean-Paul Di Rago, Zhihong Liu, Deborah Tribouillard-Tanvier, Huimei Chen
Article
Biochemistry & Molecular Biology
Xin Su, Alain Dautant, Malgorzata Rak, Francois Godard, Nahia Ezkurdia, Marine Bouhier, Mailis Bietenhader, David M. Mueller, Roza Kucharczyk, Jean-Paul Di Rago, Deborah Tribouillard-Tanvier
Summary: Human ATP synthase is composed of 29 subunits, with only two encoded in the mitochondrial genome. Mutations in the gene encoding subunit a, ATP6, have been associated with neuromuscular diseases. The aL(173)R mutation has been shown to compromise respiratory growth and decrease mitochondrial ATP synthesis.
HUMAN MOLECULAR GENETICS
(2021)
Article
Multidisciplinary Sciences
Daniel Paprocki, Maria Winiewska-Szajewska, Elzbieta Speina, Roza Kucharczyk, Jaroslaw Poznanski
Summary: The study found that iodinated compounds are an attractive alternative to brominated ones, with one of the new iodinated compounds showing superior performance in terms of cytotoxicity and mitochondrial activity, becoming a candidate leader in the further development of CK2 inhibitors.
SCIENTIFIC REPORTS
(2021)
Article
Genetics & Heredity
Anna M. Kabala, Krystyna Binko, Francois Godard, Camille Charles, Alain Dautant, Emilia Baranowska, Natalia Skoczen, Kewin Gombeau, Marine Bouhier, Hubert D. Becker, Sharon H. Ackerman, Lars M. Steinmetz, Deborah Tribouillard-Tanvier, Roza Kucharczyk, Jean-Paul Di Rago
Summary: The yeast mitochondrial ATP synthase is composed of multiple subunits, some encoded by mitochondrial genes and others by nuclear genes. Subunit 6 and 9 are involved in proton transport across the mitochondrial inner membrane and are synthesized in both cytosol and mitochondria. Translation modifications and cis-regulatory sequences play a role in the assembly and gene expression control of subunit 6 and 9.
Meeting Abstract
Biochemistry & Molecular Biology
Roza Kucharczyk, Emilia Baranowska, Katarzyna Niedzwiecka, Chiranjit Panja, Alain Dautant, Jaroslaw Poznanski, Jean-Paul di Rago, Deborah Tribouillard-Tanvier
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Anna Kabala, Krystyna Binko, Francois Godard, Alain Dautant, Emilia Baranowska, Natalia Skoczen, Kevin Gombeau, Marine Bouhier, Hubert Becker, Sharon Ackerman, Lars Steinmetz, Deborah Tribouillard-Tanvier, Roza Kucharczyk, Jean-Paul di Rago
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Gaetan Bader, Ludovic Enkler, Yuhei Araiso, Marine Hemmerle, Krystyna Binko, Emilia Baranowska, Aneta Wiesyk, Johan-Owen De Craene, Julie Ruer-Laventie, Jean Pieters, Deborah Tribouillard-Tanvier, Bruno Senger, Jean-Paul di Rago, Sylvie Friant, Hubert Dominique Becker, Roza Kucharczyk
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Chiranjit Panja, Aneta Wiesyk, Katarzyna Niedzwiecka, Emilia Baranowska, Roza Kucharczyk
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Marta Kasabula, Chiranijt Panja, Roza Kucharczyk
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Article
Biochemistry & Molecular Biology
Emilia Baranowska, Katarzyna Niedzwiecka, Chiranjit Panja, Camille Charles, Alain Dautant, Jean-Paul di Rago, Deborah Tribouillard-Tanvier, Roza Kucharczyk
Summary: The mitochondrial DNA mutation m.9032T>C was found in NARP patients, causing maternal transmission and abnormalities in mitochondrial function. This mutation leads to a disruption in proton conduction between the external side of the membrane and the c-ring of ATP synthase. Intragenic suppressors were identified, restoring ATP synthase function to some extent.
HUMAN MOLECULAR GENETICS
(2023)
Article
Cell Biology
Emilia Baranowska, Katarzyna Niedzwiecka, Chiranjit Panja, Camille Charles, Alain Dautant, Jaroslaw Poznanski, Jean-Paul di Rago, Deborah Tribouillard-Tanvier, Roza Kucharczyk
Summary: The number of mtDNA variants detected in patients with neurodegenerative diseases is increasing, making it difficult to evaluate their functional consequences and pathogenicity. Saccharomyces cerevisiae provides a convenient model to investigate the functional consequences of these variants. Yeast models of certain MT-ATP6 gene variants showed significant defects in growth and ATP production, indicating their pathogenicity, while yeast models of other variants showed mild or no effect on mitochondrial function, suggesting they do not have the potential to compromise human health.
DISEASE MODELS & MECHANISMS
(2023)
Meeting Abstract
Chemistry, Multidisciplinary
Diana R. Tomchick, Anju Sreelatha, Samantha S. Yee, Victor A. Lopez, Brenden C. Park, Lisa N. Kinch, Sylwia Pilch, Kelly A. Servage, Junmei Zhang, Jenny Jiou, Monika Karasiewicz-Urbanska, Malgorzata Lobocka, Nick V. Grishin, Kim Orth, Roza Kucharczyk, Krzysztof Pawlowski, Vincent S. Tagliabracci
ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES
(2019)