Article
Biochemistry & Molecular Biology
Abramo J. Manfredonia, Daniel A. Kraut
Summary: The ubiquitin-proteasome system is responsible for protein degradation in eukaryotic cells. The study showed that degradation of ubiquitin-independent degrons (UbIDs) is slower and relies on loosely folded substrates. Furthermore, UbID degradation is ATP-independent.
Review
Biochemistry & Molecular Biology
Wojciech Bialek, James F. Collawn, Rafal Bartoszewski
Summary: This article discusses the role of ubiquitin-mediated protein destabilization in protein degradation, as well as the significance of the ubiquitin-independent pathway and 20S proteasome. It also explores the interaction between the ubiquitin-proteasome system and pathogenic microorganisms, and how these microorganisms manipulate the system to evade or counteract host responses.
Article
Multidisciplinary Sciences
Julianna R. Cresti, Abramo J. Manfredonia, Christopher E. Braganca, Joseph A. Boscia, Christina M. Hurley, Mary D. Cundiff, Daniel A. Kraut
Summary: The 26S proteasome, responsible for protein degradation in eukaryotic cells, transitions between substrate-accepting and substrate-processing conformations, with important intramolecular interactions stabilizing these conformations. A new conformationally sensitive assay revealed that interactions involving Rpn5 and Rpn2 are crucial for stabilizing substrate-processing conformations, impacting the proteasome's ability to successfully unfold and degrade difficult substrates.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Afu Fu, Victoria Cohen-Kaplan, Noa Avni, Ido Livneh, Aaron Ciechanover
Summary: The degradation of proteins through the ubiquitin-proteasome system is a complex multistep process that relies on the coordinated activity of various enzymes. Nuclear condensates containing essential components like p62 play a crucial role in protein quality control and degradation, especially under stress conditions. These assemblies, generated through liquid-liquid phase separation, efficiently facilitate proteolysis of nuclear proteins and unassembled proteasome subunits, indicating their involvement in cellular protein quality control.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Jinjin Li, Weiwei Wang, Jinhong Yuan, Jinyu Xu, Lifei He, Xinying Zhang, Haiyan Zhang
Summary: Plants have evolved sophisticated mechanisms to adjust to nutrient deficiency or excess. The COPT2 protein in Arabidopsis thaliana is transcriptionally regulated in response to changing levels of cellular copper, but its activity is subject to degradation in response to excess copper. The protein's stability is affected by proteasome inhibition and its ubiquitination status, highlighting the complexity of plasma membrane transporter degradation mechanisms.
Article
Biochemistry & Molecular Biology
Zhefan Stephen Chen, Mingqi Yan, Wenhui Pei, Bowen Yan, Caoxing Huang, Ho Yin Edwin Chan
Summary: In this study, the potential therapeutic effect of lignin-carbohydrate complexes (LCCs) on polyglutamine diseases was investigated. LCCs isolated from bamboo and poplar were found to effectively eliminate protein aggregation and rescue photoreceptor degeneration. Mechanistically, LCCs were shown to upregulate proteasomal activity, which was crucial for their beneficial effects on polyglutamine neurotoxicity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Biochemistry & Molecular Biology
Carolyn Allain Breckel, Mark Hochstrasser
Summary: The proper folding of proteins is vital for their diverse functions, and misfolded proteins can potentially harm cells by forming aggregates. Protein quality control pathways are responsible for repairing or degrading abnormal proteins, with the ubiquitin-proteasome system being commonly employed.
Article
Multidisciplinary Sciences
Donghong Ju, Li Li, Youming Xie
Summary: Ribosomal proteins are regulated by cotranslational protein degradation, which does not require prior ubiquitylation. Cotranslational folding efficiency and expression level are critical factors determining cotranslational protein degradation of ribosomal proteins.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biochemistry & Molecular Biology
Hangjun Sun, Xinxin Jing, Chaonan Wang, Pengyue Wang, Ziting Huang, Bingjian Sun, Pengbai Li, Honglian Li, Chao Zhang
Summary: Plant viruses cause significant damage to global crop production and plants activate defense signaling pathways to hinder virus propagation. Protein homeostasis regulation plays a vital role in the ongoing battle between plants and viruses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Yanhui Zhou, Hakim Manghwar, Weiming Hu, Fen Liu
Summary: Autophagy is a key pathway for nutrient recycling in eukaryotes, influenced by various factors such as hormones, second messengers, post-transcriptional regulation, and protein post-translational modification. It is activated under stress conditions to help cells survive, and the degradation mechanism of autophagy-related proteins in different organisms has attracted attention.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Hyeongjoo Oh, Min-Kyung Kang, Sin-Hye Park, Dong Yeon Kim, Soo-Il Kim, Su Yeon Oh, Woojin Na, Jae-Hoon Shim, Soon Sung Lim, Young-Hee Kang
Summary: Asaronic acid can inhibit cellular stress caused by 7 beta-hydroxycholesterol, decrease the activation of ER stress sensors, and enhance ubiquitin-proteasomal degradation, potentially serving as a potent atheroprotective agent.
Article
Biology
Jiale Du, Sandor Babik, Yanfeng Li, Kirandeep K. Deol, Stephen J. Eyles, Jasna Fejzo, Marco Tonelli, Eric Strieter
Summary: This study reveals that UCH37 plays a positive regulatory role in protein degradation by binding to specific K48 chains and debranching them. This finding provides insights into the mechanism of protein degradation.
Article
Cell Biology
Sonia R. Singh, Moritz Meyer-Jens, Erda Alizoti, W. Clark Bacon, Gregory Davis, Hanna Osinska, James Gulick, Silke Reischmann-Duesener, Ellen Orthey, Patrick M. McLendon, Jeffery D. Molkentin, Saskia Schlossarek, Jeffrey Robbins, Lucie Carrier
Summary: The study revealed that ZNF418 activates the ALP, inhibits the UPS, and regulates genes associated with cardiomyocyte structure and function. RNA-seq analysis showed that ZNF418 also plays a role in regulating genes involved in cardiac development and/or hypertrophy.
Article
Cell Biology
Byung-Kwon Jung, Yujin Park, Boran Yoon, Jin-Sil Bae, Seung-Woo Han, Ji-Eun Heo, Dong-Eun Kim, Kwon-Yul Ryu
Summary: LCN2 is a secretory protein produced by activated microglia and reactive astrocytes under neuroinflammatory stress conditions, leading to neuronal apoptosis. This study demonstrates that LCN2 secretion can be reduced by proteasome inhibition and autophagic flux activation in reactive astrocytes stimulated with lipopolysaccharide. The results suggest that modulating LCN2 production and secretion may offer a new therapeutic approach for neuroinflammatory diseases.
Article
Cell Biology
Joanna Abi Habib, Julie Lesenfants, Nathalie Vigneron, Benoit J. van den Eynde
Summary: This review focuses on the different subtypes of proteasomes and their roles in maintaining protein homeostasis, protein degradation, and immune response. The mechanisms of ATP- and ubiquitin-dependent protein degradation are described, along with the ATP- and ubiquitin-independent degradation process. The review also discusses the generation of peptides presented by MHC class I molecules and the potential therapeutic application of proteasome modulation in autoimmune diseases.
Article
Sport Sciences
Michael F. Almeida, Carolliny M. Silva, Rodrigo S. Chaves, Nathan C. R. Lima, Renato S. Almeida, Karla P. Melo, Marilene Demasi, Tiago Fernandes, Edilamar M. Oliveira, Luis E. S. Netto, Sandra M. Cardoso, Merari F. R. Ferrari
JOURNAL OF SPORTS SCIENCES
(2018)
Article
Endocrinology & Metabolism
Caroline A. P. de Souza, Camila Congentino Gallo, Ludmilla Scodeler de Camargo, Paula Vargas Versignassi de Carvalho, Ingrid Fernandes Olescuck, Felipe Macedo, Fernanda Marques da Cunha, Jose Cipolla-Neto, Fernanda G. do Amaral
JOURNAL OF PINEAL RESEARCH
(2019)
Article
Neurosciences
Karla P. Melo, Carolliny M. Silva, Michael F. Almeida, Rodrigo S. Chaves, Tania Marcourakis, Sandra M. Cardoso, Marilene Demasi, Luis E. S. Netto, Merari F. R. Ferrari
NEUROTOXICITY RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Janaina M. M. Leme, Erina Ohara, Veronica F. Santiago, Mario H. Barros, Luis E. S. Netto, Daniel C. Pimenta, Douglas O. C. Mariano, Cristiano L. P. Oliveira, Renata N. Bicev, Maria L. M. Barreto-Chaves, Caroline A. Lino, Marilene Demasi
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2019)
Article
Cell Biology
Caroline Antunes Lino, Marilene Demasi, Maria Luiza Barreto-Chaves
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2019)
Review
Neurosciences
Felipe Macedo, Lucas Souza dos Santos, Isaias Glezer, Femanda Marques da Cunha
FRONTIERS IN NEUROSCIENCE
(2019)
Article
Biochemistry & Molecular Biology
Felipe Macedo, Gabriel Loureiro Martins, Luis A. Luevano-Martinez, Gustavo Monteiro Viana, Karin A. Riske, Alex Inague, Marcos Y. Yoshinaga, Hugo Aguilaniu, Sayuri Miyamoto, Isaias Glezer, Fernanda Marques da Cunha
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2020)
Article
Geriatrics & Gerontology
Vittoria de Lima Camandona, Rafaela Maria Rios-Anjos, Thiago Geronimo Pires Alegria, Fabio Pereira, Renata Naporano Bicev, Fernanda Marques da Cunha, Luciano Antonio Digiampietri, Mario Henrique de Barros, Luis Eduardo Soares Netto, Jose Ribamar Ferreira-Junior
Article
Biochemistry & Molecular Biology
Felipe Macedo, Talita Romanatto, Carolina Gomes de Assis, Alexia Buis, Alicia J. Kowaltowski, Hugo Aguilaniu, Fernanda Marques da Cunha
Article
Microbiology
Laure N. A. Ries, Lakhansing Pardeshi, Zhicliang Dong, Kaeling Tan, Jacob L. Steenwyk, Ana Cristina Colabardini, Jaire A. Ferreira Filho, Patricia A. de Castro, Lilian P. Silva, Nycolas W. Preite, Fausto Almeida, Leandro J. de Assis, Renato A. C. dos Santos, Paul Bowyer, Michael Bromley, Rebecca A. Owens, Sean Doyle, Marilene Demasi, Diego C. R. Hernandez, Luis Eduardo S. Netto, Monica T. Pupo, Antonis Rokas, Flavio Loures, Koon H. Wong, Gustavo H. Goldman
Article
Chemistry, Analytical
Carla S. Santos, Felipe Macedo, Alicia J. Kowaltowski, Mauro Bertotti, Patrick R. Unwin, Fernanda Marques da Cunha, Gabriel N. Meloni
Summary: By using scanning electrochemical microscopy (SECM) to measure the localized oxygen consumption rates (OCRs) in different regions of single adult Caenorhabditis elegans individuals, this study revealed marked heterogeneity of OCR along the worm, with high respiration rates at the reproductive system region. Through a self-referencing approach and genetic/pharmacological methods, SECM measurements indicate that viable eggs in the reproductive system are the main contributors to the total oxygen consumption of adult Caenorhabditis elegans, providing new insights into tissue-specific metabolic analyses and toxicity evaluation within single organisms.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Marilene Demasi, Ohara Augusto, Etelvino J. H. Bechara, Renata N. Bicev, Fernanda M. Cerqueira, Fernanda M. da Cunha, Ana Denicola, Fernando Gomes, Sayuri Miyamoto, Luis E. S. Netto, Lia M. Randall, Cassius V. Stevani, Leonor Thomson
Summary: Research on protein oxidation began in 1980, exploring the impact of oxidative modifications on protein function and cellular homeostasis. Recent studies have shown that not all oxidative modifications result in damage, but can also alter protein structure and function. Understanding the effects of protein oxidation on human pathological conditions is clinically significant.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Multidisciplinary Sciences
Renata Naporano Bicev, Maximilia Frazao de Souza Degenhardt, Cristiano Luis Pinto de Oliveira, Emerson Rodrigo da Silva, Jeril Degrouard, Guillaume Tresset, Graziella Eliza Ronsein, Marilene Demasi, Fernanda Marques da Cunha
Summary: Glucose restriction enhances the activity of proteasomes in yeast cells, improving their ability to degrade proteins, possibly through phosphorylation of the proteasome subunit. However, this enhancement in activity does not result from changes in the conformation of the proteasome particles.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
A. Prabakaran, Amit Alexander
Summary: The molecular interactions and mucoadhesive nature of chitosan-coated liposomes with mucin are crucial for the development of an effective drug delivery system. The positively charged SA-CH-LPs showed stronger interaction and better mucoadhesive properties compared to negatively charged SALPs, thereby improving drug retention in the nasal cavity and enhancing therapeutic efficacy.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Maryam Noei-Khesht Masjedi, Esmaeil Sadroddiny, Jafar Ai, Saeed Balalaie, Yazdan Asgari
Summary: This study discovered an effective strategy for cargo sorting within exosomes by incorporating an appropriate cleavage site, providing further insight into the potential of exosomes as nano-shuttles bearing therapeutic biomolecules.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Pei -Gee Yap, Chee-Yuen Gan
Summary: This study collected the sequences of 128 tyrosinase inhibitory peptides and analyzed their hydrophobicity/hydrophilicity properties and amino acid profiles. Molecular docking analysis was used to investigate the binding interactions between peptides and tyrosinase. The study found that hydrophobic and/or polar neutral properties facilitate or stabilize peptide binding with tyrosinase, and short peptides with cysteine and tyrosine tend to bind to the active site of tyrosinase. These findings provide detailed explanations for the relationship between peptide/amino acid structures and tyrosinase inhibition, as well as potential anti-melanogenesis mechanisms for peptide-based treatments against skin hyperpigmentation.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ye Yang, Hai-Lian Chen, Su Fang Wu, Wei Bao
Summary: The study found that CHMP4B and VPS4A play an important role in reversing GSDMD-mediated pyroptosis by facilitating cell membrane remodeling in endometrial carcinoma.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aya Al Othman, Dmitry Bagrov, Julian M. Rozenberg, Olga Glazova, Gleb Skryabin, Elena Tchevkina, Alexandre Mezentsev, Mikhail Durymanov
Summary: Arc protein is found in glutamatergic neurons of vertebrates and can be transferred between neurons in extracellular vesicles (EVs). In glioma cells, Arc protein is also present in EVs and can facilitate the transfer of mRNA, potentially contributing to tumor progression and affecting synaptic plasticity in cancer patients.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Nan Zhang, Meng-yu Shen, Qing-li Meng, Hao-ping Sun, Fang-yi Fan, Hai Yi, Yong-jian Yang
Summary: In this study, it was demonstrated for the first time that FAT1 inhibited AML proliferation by reducing autophagy level. FAT1 achieved this by decreasing the expression of ATG4B, which is related to autophagy. Mechanistically, FAT1 decreased the levels of phosphorylated and intranuclear smad2/3, thus decreasing the activity of the ATG4B gene promoter. It was also found that FAT1 competitively bound to TGF-beta R II, leading to reduced phosphorylation of TGF-beta R I and smad2/3. Knockdown of FAT1 promoted AML autophagy and proliferation in vivo.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Oleh Demianchuk, Myroslava Vatashchuk, Dmytro Gospodaryov, Viktoria Hurza, Marian Ivanochko, Vitalii Derkachov, Vladyslav Berezovskyi, Oleh Lushchak, Kenneth B. Storey, Maria Bayliak, Volodymyr I. Lushchak
Summary: This study investigated the effects of a high-fat high-fructose diet (HFFD) on the behavior, energy metabolism, and oxidative stress markers in the cerebral cortex of mice. The results showed that HFFD stimulated locomotion and defecation, while an AKG-supplemented diet had a tendency to promote anxiety-like behavior. Additionally, there were significant differences in glutathione-dependent detoxification and processes related to autophagy between the two diets.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Yusaku Chukai, Ginga Ito, Yasuo Miki, Koichi Wakabayashi, Ken Itoh, Eriko Sugano, Hiroshi Tomita, Tomokazu Fukuda, Taku Ozaki
Summary: The study found that mitochondrial calpain-5 plays an important role in the occurrence and development of ischemia-reperfusion injury and is expressed in the human and mouse brains. Targeting the expression or activity of mitochondrial calpain-5 may have significant implications for suppressing inflammation during I/R injuries such as cerebrovascular diseases.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aline Dias da Purificaca, Victor Debbas, Leonardo Yuji Tanaka, Gabriele Veronica de Mello Gabriel, Joao Wosniak Junior, Tiphany Coralie De Bessa, Sheila Garcia-Rosa, Francisco Rafael Martins Laurindo, Percillia Victoria Santos Oliveira
Summary: The ER transmembrane chaperones DNAJB12 and DNAJB14 play important roles in protein folding and ER stress response. They are regulated by thiol redox processes and are involved in ER protein reflux.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ekaterina O. Bryanskaya, Andrey Y. Vinokurov, Angelina I. Dolgikh, Andrey Dunaev, Plamena R. Angelova, Andrey Y. Abramov
Summary: FAD autofluorescence in cells can assess enzymatic activity, and its intensity variations may be related to different cell types and tissues. High levels of FAD autofluorescence can indicate cell pathology and potentially predict the occurrence of apoptosis and necrosis.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Rumei Luan, Manyu Luo, Dongyan Ding, Xin Su, Junling Yang
Summary: Zinc deficiency can worsen obesity-related lung damage, and Nrf2 activation is one of the important mechanisms of this protective effect. Regulating zinc homeostasis can contribute to the prevention and treatment of obesity-related lung injury.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
