Reversible inhibition of the ClpP protease via an N-terminal conformational switch

Simultaneous Determination of Protein Structure and Dynamics Using Cryo-Electron Microscopy

(2018) Massimiliano Bonomi et al.   BIOPHYSICAL JOURNAL

Loss of mitochondrial protease ClpP protects mice from diet‐induced obesity and insulin resistance

(2018) Shylesh Bhaskaran et al.   EMBO REPORTS

NMR Studies of Large Proteins

(2017) Yajun Jiang et al.   JOURNAL OF MOLECULAR BIOLOGY

The development of small-molecule modulators for ClpP protease activity

(2017) Fei Ye et al.   Molecular BioSystems

Conformational control of the bacterial Clp protease by natural product antibiotics

(2017) I. T. Malik et al.   NATURAL PRODUCT REPORTS

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

Probing the cooperativity ofThermoplasma acidophilumproteasome core particle gating by NMR spectroscopy

(2017) Rui Huang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ratchet-like polypeptide translocation mechanism of the AAA+ disaggregase Hsp104

(2017) Stephanie N. Gates et al.   SCIENCE

Structural basis of protein translocation by the Vps4-Vta1 AAA ATPase

(2017) Nicole Monroe et al.   eLife

Characterization of Gain-of-Function Mutant Provides New Insights into ClpP Structure

(2016) Tengfeng Ni et al.   ACS Chemical Biology

ClpP-deletion impairs the virulence of Legionella pneumophila and the optimal translocation of effector proteins

(2016) Bei-bei Zhao et al.   BMC MICROBIOLOGY

Bacterial proteases, untapped antimicrobial drug targets

(2016) Elizabeth Culp et al.   JOURNAL OF ANTIBIOTICS

Structure and Functional Properties of the Active Form of the Proteolytic Complex, ClpP1P2, fromMycobacterium tuberculosis

(2016) Mi Li et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Unfolding the mechanism of the AAA+ unfoldase VAT by a combined cryo-EM, solution NMR study

(2016) Rui Huang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Open and compressed conformations ofFrancisella tularensisClpP

(2016) Laura Díaz-Sáez et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

The Mitochondrial Unfoldase-Peptidase Complex ClpXP Controls Bioenergetics Stress and Metastasis

(2016) Jae Ho Seo et al.   PLOS BIOLOGY

Metainference: A Bayesian inference method for heterogeneous systems

(2016) M. Bonomi et al.   Science Advances

Metadynamic metainference: Enhanced sampling of the metainference ensemble using metadynamics

(2016) Massimiliano Bonomi et al.   Scientific Reports

Inhibition of the Mitochondrial Protease ClpP as a Therapeutic Strategy for Human Acute Myeloid Leukemia

(2015) Alicia Cole et al.   CANCER CELL

Methyl-specific isotopic labeling: a molecular tool box for solution NMR studies of large proteins

(2015) Rime Kerfah et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Phenyl Esters Are Potent Inhibitors of Caseinolytic Protease P and Reveal a Stereogenic Switch for Deoligomerization

(2015) Mathias W. Hackl et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Mechanistic insights into the recycling machine of the SNARE complex

(2015) Minglei Zhao et al.   NATURE

Mechanistic insights into bacterial AAA+ proteases and protein-remodelling machines

(2015) Adrian O. Olivares et al.   NATURE REVIEWS MICROBIOLOGY

Assaying the kinetics of protein denaturation catalyzed by AAA+ unfolding machines and proteases

(2015) Vladimir Baytshtok et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

AAA+ chaperones and acyldepsipeptides activate the ClpP protease via conformational control

(2015) Malte Gersch et al.   Nature Communications

Bringing Dynamic Molecular Machines into Focus by Methyl-TROSY NMR

(2014) Rina Rosenzweig et al.   Annual Review of Biochemistry

Dynamics of the ClpP serine protease: A model for self-compartmentalized proteases

(2014) Kaiyin Liu et al.   CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY

Perrault Syndrome Is Caused by Recessive Mutations in CLPP, Encoding a Mitochondrial ATP-Dependent Chambered Protease

(2013) Emma M. Jenkinson et al.   AMERICAN JOURNAL OF HUMAN GENETICS

Mutations in LARS2, Encoding Mitochondrial Leucyl-tRNA Synthetase, Lead to Premature Ovarian Failure and Hearing Loss in Perrault Syndrome

(2013) Sarah B. Pierce et al.   AMERICAN JOURNAL OF HUMAN GENETICS

Helix Unfolding/Refolding Characterizes the Functional Dynamics ofStaphylococcus aureusClp Protease

(2013) Fei Ye et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Nmrglue: an open source Python package for the analysis of multidimensional NMR data

(2013) Jonathan J. Helmus et al.   JOURNAL OF BIOMOLECULAR NMR

Activation of ClpP Protease by ADEP Antibiotics: Insights from Hydrogen Exchange Mass Spectrometry

(2013) Modupeola A. Sowole et al.   JOURNAL OF MOLECULAR BIOLOGY

One number does not fit all: Mapping local variations in resolution in cryo-EM reconstructions

(2013) Giovanni Cardone et al.   JOURNAL OF STRUCTURAL BIOLOGY

Activated ClpP kills persisters and eradicates a chronic biofilm infection

(2013) B. P. Conlon et al.   NATURE

Insights into Structural Network Responsible for Oligomerization and Activity of Bacterial Virulence Regulator Caseinolytic Protease P (ClpP) Protein

(2012) Malte Gersch et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

ClpP: A structurally dynamic protease regulated by AAA+ proteins

(2012) John A. Alexopoulos et al.   JOURNAL OF STRUCTURAL BIOLOGY

Studying “Invisible” Excited Protein States in Slow Exchange with a Major State Conformation

(2012) Pramodh Vallurupalli et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Life and Death of Proteins: A Case Study of Glucose-starvedStaphylococcus aureus

(2012) Stephan Michalik et al.   MOLECULAR & CELLULAR PROTEOMICS

Bacterial proteolytic complexes as therapeutic targets

(2012) Ravikiran M. Raju et al.   NATURE REVIEWS DRUG DISCOVERY

Proteasome allostery as a population shift between interchanging conformers

(2012) A. M. Ruschak et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mycobacterium tuberculosis ClpP1 and ClpP2 Function Together in Protein Degradation and Are Required for Viability in vitro and During Infection

(2012) Ravikiran M. Raju et al.   PLoS Pathogens

A Conformational Switch Underlies ClpP Protease Function

(2011) Sebastian R. Geiger et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Development and characterization of improved β-lactone-based anti-virulence drugs targeting ClpP

(2011) Evelyn Zeiler et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Activators of Cylindrical Proteases as Antimicrobials: Identification and Development of Small Molecule Activators of ClpP Protease

(2011) Elisa Leung et al.   CHEMISTRY & BIOLOGY

Structural Switching ofStaphylococcus aureusClp Protease

(2011) Jie Zhang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

SHIFTX2: significantly improved protein chemical shift prediction

(2011) Beomsoo Han et al.   JOURNAL OF BIOMOLECULAR NMR

An Optimized Relaxation-Based Coherence Transfer NMR Experiment for the Measurement of Side-Chain Order in Methyl-Protonated, Highly Deuterated Proteins

(2011) Hechao Sun et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Site-Directed Methyl Group Labeling as an NMR Probe of Structure and Dynamics in Supramolecular Protein Systems: Applications to the Proteasome and to the ClpP Protease

(2011) Tomasz L. Religa et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structural insights into the conformational diversity of ClpP from Bacillus subtilis

(2011) Byung-Gil Lee et al.   MOLECULES AND CELLS

Antibiotic acyldepsipeptides activate ClpP peptidase to degrade the cell division protein FtsZ

(2011) P. Sass et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Stereospecific Isotopic Labeling of Methyl Groups for NMR Spectroscopic Studies of High-Molecular-Weight Proteins

(2010) Pierre Gans et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Binding of the ClpA Unfoldase Opens the Axial Gate of ClpP Peptidase

(2010) Grégory Effantin et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Control of Substrate Gating and Translocation into ClpP by Channel Residues and ClpX Binding

(2010) Mary E. Lee et al.   JOURNAL OF MOLECULAR BIOLOGY

Determination of Isoleucine Side-Chain Conformations in Ground and Excited States of Proteins from Chemical Shifts

(2010) D. Flemming Hansen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structures of ClpP in complex with acyldepsipeptide antibiotics reveal its activation mechanism

(2010) Byung-Gil Lee et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Dynamic Regulation of Archaeal Proteasome Gate Opening As Studied by TROSY NMR

(2010) T. L. Religa et al.   SCIENCE

Structural and Theoretical Studies Indicate that the Cylindrical Protease ClpP Samples Extended and Compact Conformations

(2010) Matthew S. Kimber et al.   STRUCTURE

The antibiotic ADEP reprogrammes ClpP, switching it from a regulated to an uncontrolled protease

(2009) Janine Kirstein et al.   EMBO Molecular Medicine

Clp chaperone–proteases: structure and function

(2009) Wolfgang Kress et al.   RESEARCH IN MICROBIOLOGY

β-Lactones as Privileged Structures for the Active-Site Labeling of Versatile Bacterial Enzyme Classes

(2008) Thomas Böttcher et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

ClpP Hydrolyzes a Protein Substrate Processively in the Absence of the ClpA ATPase: Mechanistic Studies of ATP-Independent Proteolysis†

(2008) Laura D. Jennings et al.   BIOCHEMISTRY

The Structural Basis for the Activation and Peptide Recognition of Bacterial ClpP

(2008) Dong Young Kim et al.   JOURNAL OF MOLECULAR BIOLOGY

Flexible Fitting of Atomic Structures into Electron Microscopy Maps Using Molecular Dynamics

(2008) Leonardo G. Trabuco et al.   STRUCTURE