Optimized purification of mono-PEGylated lysozyme by heparin affinity chromatography using response surface methodology

Quality by Design (QbD)-Based Process Development for Purification of a Biotherapeutic

(2016) Anurag S. Rathore   TRENDS IN BIOTECHNOLOGY

Exploring the use of heparin as a first capture step in the purification of monoclonal antibodies from cell culture supernatants

(2015) Inês F. Pinto et al.   BIOCHEMICAL ENGINEERING JOURNAL

Toward improving selectivity in affinity chromatography with PEGylated affinity ligands: The performance of PEGylated protein A

(2014) José González-Valdez et al.   BIOTECHNOLOGY PROGRESS

Separation of PEGylated variants of ribonuclease A and apo-α-lactalbumin via reversed phase chromatography

(2014) Mayra Cisneros-Ruiz et al.   JOURNAL OF CHROMATOGRAPHY A

Process for protein PEGylation

(2014) David Pfister et al.   JOURNAL OF CONTROLLED RELEASE

Chemoenzymatic synthesis of heparan sulfate and heparin

(2014) Jian Liu et al.   NATURAL PRODUCT REPORTS

Chemical grafting of Sepharose 6B and its use in the purification of PEGylated RNase A

(2014) Agustín Hernández-Martínez et al.   SEPARATION AND PURIFICATION TECHNOLOGY

Purifying biopharmaceuticals: knowledge-based chromatographic process development

(2014) Alexander T. Hanke et al.   TRENDS IN BIOTECHNOLOGY

Design of experiments applications in bioprocessing: Concepts and approach

(2013) Vijesh Kumar et al.   BIOTECHNOLOGY PROGRESS

Microcalorimetric study of the adsorption of native and mono-PEGylated bovine serum albumin on anion-exchangers

(2013) Tim Blaschke et al.   JOURNAL OF CHROMATOGRAPHY A

Adsorption of polyethylene-glycolated bovine serum albumin on macroporous and polymer-grafted anion exchangers

(2013) Mimi Zhu et al.   JOURNAL OF CHROMATOGRAPHY A

PEGylated protein separations: Challenges and opportunities

(2012) Noriko Yoshimoto et al.   Biotechnology Journal

Hydrophobic interaction chromatography for purification of monoPEGylated RNase A

(2012) Karla Mayolo-Deloisa et al.   JOURNAL OF CHROMATOGRAPHY A

Experimental design in chromatography: A tutorial review

(2012) D. Brynn Hibbert   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Pharmaceutical and biomedical applications of affinity chromatography: Recent trends and developments

(2012) David S. Hage et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Microcalorimetric Study of the Adsorption of PEGylated Lysozyme and PEG on a Mildly Hydrophobic Resin: Influence of Ammonium Sulfate

(2012) Albert Werner et al.   LANGMUIR

Determination of robustness and optimal work conditions for a purification process of a therapeutic recombinant protein using response surface methodology

(2011) Ignacio Amadeo et al.   BIOTECHNOLOGY PROGRESS

Microcalorimetric study of the adsorption of PEGylated lysozyme on a strong cation exchange resin

(2011) Tim Blaschke et al.   JOURNAL OF CHROMATOGRAPHY A

Preparation and characterization of PEGyated Concanavalin A for affinity chromatography with improved stability

(2011) Zhenzhen Wen et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

FDA-approved poly(ethylene glycol)–protein conjugate drugs

(2011) Steevens N. S. Alconcel et al.   Polymer Chemistry

Human Serum Protein Adsorption onto Synthesis Nano-Hydroxyapatite

(2011) M. Mohsen-Nia et al.   PROTEIN JOURNAL

Interaction mechanism of mono-PEGylated proteins in electrostatic interaction chromatography

(2010) Mitsuyo Abe et al.   Biotechnology Journal

PEGylation of therapeutic proteins

(2010) Simona Jevševar et al.   Biotechnology Journal

Biochemical and biophysical characterization of lysozyme modified by PEGylation

(2010) Débora da Silva Freitas et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

Insights into the modeling of adsorption isotherm systems

(2009) K.Y. Foo et al.   CHEMICAL ENGINEERING JOURNAL

Analytical and preparative separation of PEGylated lysozyme for the characterization of chromatography media

(2009) Anna Moosmann et al.   JOURNAL OF CHROMATOGRAPHY A

Quality by design for biopharmaceuticals

(2009) Anurag S Rathore et al.   NATURE BIOTECHNOLOGY

Separation of PEGylated from unmodified ribonuclease A using sepharose media

(2008) Mayra Cisneros-Ruiz et al.   SEPARATION AND PURIFICATION TECHNOLOGY

Heparin chromatography to deplete high-abundance proteins for serum proteomics

(2007) Ting Lei et al.   CLINICA CHIMICA ACTA