Article
Biochemistry & Molecular Biology
Sirui Zhou, Guillaume Butler-Laporte, Tomoko Nakanishi, David R. Morrison, Jonathan Afilalo, Marc Afilalo, Laetitia Laurent, Maik Pietzner, Nicola Kerrison, Kaiqiong Zhao, Elsa Brunet-Ratnasingham, Danielle Henry, Nofar Kimchi, Zaman Afrasiabi, Nardin Rezk, Meriem Bouab, Louis Petitjean, Charlotte Guzman, Xiaoqing Xue, Chris Tselios, Branka Vulesevic, Olumide Adeleye, Tala Abdullah, Noor Almamlouk, Yiheng Chen, Michael Chasse, Madeleine Durand, Clare Paterson, Johan Normark, Robert Frithiof, Miklos Lipcsey, Michael Hultstrom, Celia M. T. Greenwood, Hugo Zeberg, Claudia Langenberg, Elin Thysell, Michael Pollak, Vincent Mooser, Vincenzo Forgetta, Daniel E. Kaufmann, J. Brent Richards
Summary: The study identified that increased levels of OAS1 protein are associated with reduced susceptibility and severity of COVID-19, providing evidence for a protective role of OAS1 in adverse outcomes. This suggests that pharmacological agents that increase OAS1 levels could be prioritized for drug development against COVID-19. The variant of the OAS1 gene associated with decreased risk of death in COVID-19 patients encodes an enzyme critical for the innate immune response to viral infections.
Article
Biotechnology & Applied Microbiology
Victor R. Campos-Garcia, Jorge Benavides, Jose Gonzalez-Valdez
Summary: This study successfully demonstrates the production and purification of PEGylated proteins with enhanced biological activity using reactive ATPS systems, presenting a novel approach for simultaneous synthesis and purification.
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Chintan Savla, Andre F. Palmer
Summary: By binding heme to human serum albumin and stabilizing it with polyethylene glycol, the anti-inflammatory effects of heme can be realized. This study provides a method for the synthesis and purification of heme protein carriers.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Medicine, General & Internal
Juan Carlos Cordova, Sheng Sun, Jeffrey Bos, Srinath Thirumalairajan, Sanjeevani Ghone, Miyako Hirai, Ricarda A. Busse, Julia S. v. der Hardt, Ian Schwartz, Jieyu Zhou
Summary: Membrane chromatography, specifically utilizing Sartobind(R) S and Phenyl membranes, is used to remove aggregates and excess payload in ADC downstream processing. This approach effectively improves the purity of ADCs by clearing unwanted species and increasing homogeneity of drug-to-antibody ratio (DAR).
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Rakesh Kumar, Sunita Katwal, Bhupender Sharma, Abhishek Sharma, Sanjeev Puri, Nidhi Kamboj, Shamsher Singh Kanwar
Summary: The RNase produced by B. safensis RB-5 was purified using various techniques and showed optimal activity and antiproliferative effects under specific conditions, while also exhibiting cytotoxicity towards certain transformed cell lines.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Allyson Tucker Dilks, Joanne Gilchrist, Yik Lam, Nathan Nicholes, Brad Stanley
Summary: No-salt flowthrough hydrophobic interaction chromatography (HIC) is effective in removing impurities from bioprocess streams. This study demonstrates the operating principles of no-salt flowthrough HIC in antibody purification, showing robust aggregate clearance across various conditions. HMW reduction can be improved by adjusting protein load and/or concentration.
BIOTECHNOLOGY PROGRESS
(2023)
Article
Biochemistry & Molecular Biology
Bijoy K. Mohanty, Keri Nichols, Sidney R. Kushner
Summary: This study reveals a complex maturation process for Glu2, Ile1, and Ala1B tRNAs encoded within the rRNA operons in Escherichia coli, which is distinct from previously characterized tRNA processing pathways in Gram-positive bacteria.
MOLECULAR MICROBIOLOGY
(2022)
Article
Plant Sciences
Hansol Bae, Magnus Paludan, Jan Knoblauch, Kaare H. Jensen
Summary: Plant metabolites are crucial chemicals with diverse functional properties. By utilizing neural networks, we are able to identify and extract metabolite-rich cells from herbal plants, offering a new approach to enhance the efficiency of plant metabolites.
Article
Biochemistry & Molecular Biology
Shing Fung Lam, Xiaojiao Shang, Raja Ghosh
Summary: This paper presents a microfiltration membrane-based hybrid method for purifying PEGylated proteins, which involves converting the proteins into micellar form using a lyotropic salt and separating them from impurities using a microfiltration membrane. The retained proteins on the membrane can be recovered by removing the lyotropic salt, and different PEGylated forms can be fractionated by controlling the salt removal. The method is shown to be fast and scalable through the use of two different types of microfiltration membrane devices.
Article
Biochemistry & Molecular Biology
Guillem Prats-Ejarque, Helena Lorente, Clara Villalba, Raul Anguita, Lu Lu, Sergi Vazquez-Monteagudo, Pablo Fernandez-Millan, Ester Boix
Summary: Bacterial resistance to antibiotics has led to the development of a hybrid enzyme with high antimicrobial and catalytic activities. After multiple improvements, the RNase 3/1-v3 chimera was designed, which exhibited enhanced antimicrobial and catalytic activities, as well as low cytotoxicity. Furthermore, the RNase 3/1-v3 chimera was able to effectively eradicate intracellular infection in macrophages and induce autophagy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Maria C. Martinez Ceron, Lucia Avila, Silvana L. Giudicessi, Juan M. Minoia, Matias Fingermann, Silvia A. Camperi, Fernando Albericio, Osvaldo Cascone
Summary: A fully automated two-stage library screening method was applied to identify peptide ligands suitable for tetanus toxoid purification, achieving a highly efficient purification of T-tx.
Review
Biochemical Research Methods
Joselyn S. Soto, Yasaman Jami-Alahmadi, James A. Wohlschlegel, Baljit S. Khakh
Summary: This protocol provides a method for labeling and identifying cell-specific and subcompartment-specific proteins within intact astrocytes and neurons. The approach utilizes the proximity-dependent biotinylation system BioID2 to selectively label proximal proteins in cells. This protocol can be applied to any area of the central nervous system and is important for physiological and disease-related research.
Article
Chemistry, Multidisciplinary
Leila Khazdooz, Amin Zarei, Gopinathan Meletharayil, Rohit Kapoor, Alireza Abbaspourrad
Summary: Lactoferrin (LF), the main iron-binding protein in milk, has nutritional, biological, and pharmaceutical properties. Extracting lactoferrin from whey, a byproduct of the dairy industry, can have economic and environmental benefits. The synthesis of a strong cation-exchange resin for selective extraction of lactoferrin from whey is presented.
Article
Chemistry, Analytical
Songbo Xie, Jingrui Li, Shuang Sun, Wei Chen, Haisu Cheng, Yinlong Song, Yuyang Li, Min Liu, Xueliang Zhu, Xin Liang, Jun Zhou
Summary: This study developed a peptide probe called TUBright that can label in vitro reconstituted microtubules with high signal-to-noise ratio. TUBright does not interfere with the dynamic behaviors of microtubules and microtubule-associated proteins.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jessica Asami, Bruna Quevedo, Arnaldo R. Santos Jr, Luciana Pastena Giorno, Daniel Komatsu, Eliana Aparecida de Rezende Duek
Summary: This investigation examines the impact of the deproteinization process on the chemical and biological dynamics of natural rubber latex. The results show that the deproteinization process can affect the swelling kinetics, cell adhesion, surface charge, and surface morphology of the latex. The study suggests that the proteins in latex serum have potential usage in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biotechnology & Applied Microbiology
Alonso Ornelas-Gonzalez, Mirna Gonzalez-Gonzalez, Marco Rito-Palomares
Summary: Stem cell-based therapies have emerged as an alternative for treating various diseases, with the use of microcarrier culture techniques showing promise for expanding stem cells. However, challenges in production and medical applications remain, highlighting the need for the development of new bioprocesses to simplify stem cell cultures.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Mirna Gonzalez-Gonzalez, Guillermo Pico, Alvaro Silva Lima, Cassamo Ussemane Mussagy, Jorge Fernando Brandao Pereira, Hector Marcelo Fernandez-Lahore, Juan A. Asenjo, Marco Rito-Palomares
Summary: Aqueous two-phase systems (ATPS) are a simple and robust bioprocessing technology with high efficiency for concentration, enrichment, recovery, and purification of biological molecules. Four relevant countries in Latin America have played important roles in the practical evolution of ATPS. Novel applications of ATPS are being explored for visionary therapeutic tasks in healthcare, biotechnology, pharmaceutical sectors, and environmental purposes.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Mirna Gonzalez-Gonzalez, Marco Rito-Palomares
Summary: Bioseparation is a crucial process in obtaining biological products, with researchers focusing on optimizing upstream and downstream processes leading to a technological revolution. This perspective article summarises recent advances, challenges, and future trends in bioseparation, and proposes a classification of different technologies.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Review
Endocrinology & Metabolism
Margarita Ortiz-Martinez, Mirna Gonzalez-Gonzalez, Alexandro J. Martagon, Victoria Hlavinka, Richard C. Willson, Marco Rito-Palomares
Summary: Diabetes mellitus is a complex chronic illness characterized by elevated blood glucose levels, with poor control leading to various complications. Type 2 diabetes represents the most common form and its incidence is increasing globally, particularly among children and young adults due to lifestyle factors. Early detection through cost-effective screening strategies and development of new biomarkers is crucial to prevent life-threatening complications.
CURRENT DIABETES REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Alonso Ornelas-Gonzalez, Mirna Gonzalez-Gonzalez, Marco Rito-Palomares
Summary: The study demonstrates the flexibility of an enzyme-based colorimetric system for the accurate quantification of various clinically relevant biomolecules. The optimized system shows excellent linearity, precision, and sensitivity, with low variability and high recovery rates. This multi-enzyme platform has great potential for quantifying different substrates in non-conventional biofluids.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Editorial Material
Biotechnology & Applied Microbiology
Marco Rito-Palomares, Guillermo Quijano
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Medicinal
Allison Leyton, Carolina Shene, Yusuf Chisti, Juan A. Asenjo
Summary: The carotenogenic thraustochytrid Thraustochytrium sp. RT2316-16 was grown in batch and repeated-batch cultures using different feeds containing glucose, glycerol, and yeast extract, for the production of lipids, phospholipids, and carotenoids. The composition of carotenoids depended strongly on the composition of the feed.
Article
Biochemistry & Molecular Biology
David Medina-Ortiz, Sebastian Contreras, Juan Amado-Hinojosa, Jorge Torres-Almonacid, Juan A. Asenjo, Marcelo Navarrete, Alvaro Olivera-Nappa
Summary: This study proposes a method to improve the performance of predictive models in protein engineering by applying physicochemical properties for encoding strategies. Through partitioning the AAIndex database into semantically-consistent groups and using non-linear PCA to define encoders, the authors demonstrate that models using these encoders outperform classical approaches in protein and peptide function prediction, folding prediction, and biological activity prediction. Additionally, a methodology to create new sequences with desired properties is proposed. Overall, this study provides simple ways to enhance predictive tasks in protein engineering without increasing complexity.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Medicine, Research & Experimental
Karen Y. Orostica, Juan Saez-Hidalgo, Pamela R. de Santiago, Solange Rivas, Sebastian Contreras, Gonzalo Navarro, Juan A. Asenjo, Alvaro Olivera-Nappa, Ricardo Armisen
Summary: This study found that tumor stage is the primary feature that affects metastasis prediction in lung cancer patients, but the information from total mutational load (TML) also contributes to the classification. Combining clinical variables and TML can more accurately assess the likelihood of lung cancer patients progressing to metastatic states, highlighting the need for comprehensive diagnostic tools for metastasis.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Rishi Vachaspathy Astakala, Gagan Preet, Bruce F. Milne, Julius Tibyangye, Valeria Razmilic, Jean Franco Castro, Juan A. Asenjo, Barbara Andrews, Rainer Ebel, Marcel Jaspars
Summary: A new antibiotic, Mutactimycin AP, belonging to the anthracycline-type family of antibiotics was discovered from a Saccharothrix sp. isolated from the rhizosphere of lupine plants in the extreme hyper-arid Atacama Desert. The compound showed activity against MRSA and bacteria associated with bovine mastitis.
Article
Chemistry, Medicinal
Diego Valdebenito, Sebastian Urrutia, Allison Leyton, Yusuf Chisti, Juan A. Asenjo, Carolina Shene
Summary: This study investigates the effects of different amino acids in the culture medium on the growth and lipid synthesis of Thraustochytrium sp. RT2316-16. The results show that lysine, alanine, or serine can increase the content of omega-3 polyunsaturated fatty acids (PUFA) in the lipids. The use of organic ammonium and glucose in the culture medium also affects lipid accumulation and mobilization. The findings suggest a possible relationship between carotenoid and lipid metabolism, and provide guidance for enhancing omega-3 PUFA in the biomass.
Article
Chemistry, Analytical
Calef Sanchez-Trasvina, Jose Daniel Galindo-Estrada, Raunel Tinoco-Valencia, Leobardo Serrano-Carreon, Marco Rito-Palomares, Richard C. Willson, Karla Mayolo-Deloisa
Summary: This study characterized the laccase-luminol system and determined its kinetic parameters. The system can produce chemiluminescence without an intermediate molecule, but it exhibits substrate-inhibition behavior. Low concentrations of luminol can avoid this inhibition effect. In summary, the laccase-luminol system has potential applications in molecular detection and quantification.
Article
Materials Science, Multidisciplinary
Adriana R. Kyvik, Ramon Roca-Pinilla, Karla Mayolo-Deloisa, Xavier Rodriguez Rodriguez, Marc Martinez-Miguel, Marta Martos, Mariana Kober, Nora Ventosa, Jaume Veciana, Judith Guasch, Elena Garcia-Fruitos, Anna Aris, Imma Ratera
Summary: In this study, novel biofunctionalized gold surfaces with the antimicrobial multidomain recombinant protein JAMF1 were developed. The immobilized JAMF1 protein successfully reduced biofilm formation of both Escherichia coli and carbapenem-resistant Klebsiella pneumoniae. This strategy provides new possibilities for biomolecule immobilization in fundamental biological studies and biotechnological applications.
MATERIALS ADVANCES
(2023)
Article
Multidisciplinary Sciences
Luisaldo Sandate-Flores, Jose Rodriguez-Rodriguez, Magdalena Rostro-Alanis, Jorge Alejandro Santiago Urbina, Karla Mayolo-Deloisa, Elda M. Melchor-Martinez, Juan Eduardo Sosa-Hernandez, Roberto Parra-Saldivar, Hafiz M. N. Iqbal
Summary: Consumer interest in food with natural ingredients has increased, leading to research on new sources and green extraction methods. Betalains, compounds responsible for cacti fruits' color, are being extracted from jiotilla Escontria chiotilla using ATPS. Factors like TL and Vr significantly affect the yield, offering a new approach for the valorization of environmentally related plant waste materials.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)