Article
Engineering, Chemical
Siyu Song, Guohua Gu, Weiqin Huang, Yanhong Wang
Summary: This study identified the mechanisms of chalcopyrite flotation with high sodium bentonite content using saline water with divalent cations, Ca2+ and Mg2+. The decrease in pulp viscosity significantly enhanced copper recovery and grade at a certain concentration of divalent cations, while non-selective aggregation of minerals also affected copper grade. The findings suggest that the ion exchange interaction complicates the control of divalent cations concentration in industry flotation, but within a suitable range, both copper grade and recovery can be enhanced.
MINERALS ENGINEERING
(2021)
Article
Multidisciplinary Sciences
Christopher A. Pohl
Summary: The preparation of columns using electrostatic attachment of anion exchange latex particles with charge density gradients can enhance chromatographic performance at low linear velocity. Significant improvement in chromatographic performance is observed during gradient elution chromatography when multiple successive charge density gradients are prepared along the length of the column with the highest capacity oriented at the inlet end of the column.
Article
Engineering, Chemical
Xiaofeng Shi, Xianmin Mai, Renbo Wei, Yong Ma, Nithesh Naik, Zhenfeng He, Yanjun Chen, Chao Wang, Binbin Dong, Zhanhu Guo
Summary: Fe-Mg-BDC effectively removes Pb(II), while Fe-Pb(Mg)-BDC saturated with Pb(II) can rapidly remove As(V) and has a good adsorption capacity at low concentrations, allowing for the control of As(V) concentration below drinking water standards, with good renewable ability.
Article
Engineering, Chemical
Maonan Zhang, Qing Xia, Xiaodan Zhao, Jiaming Guo, Li Zeng, Zhen Zhou
Summary: The concentration effects of Ca2+ on APAM fouling were systematically investigated. The results showed that Ca2+ could alleviate APAM fouling and change the structure of the fouled layer. The study provides new insights into the concentration effects of Ca2+ on polymer fouling of membranes.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Engineering, Environmental
Xiaoying Chi, Lixuan Zeng, Yuejin Du, Jiaquan Huang, Yuan Kang, Jiwen Luo, Qiuyun Zhang
Summary: The persistence of antibiotics in sewage treatment plants has become a serious problem in recent years. In this study, natural zeolite (NZ) was evaluated as an adsorbent for the removal of levofloxacin (LEV). The effects of ammonia nitrogen and humic acid (HA) on the interfacial behavior of LEV on NZ were explored. The study found that low concentrations of ammonia nitrogen and HA are conducive to improving the treatment effect of sewage.
WATER SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Matthew J. Sorensen, Mellie June Paulines, Todd D. Maloney
Summary: This study evaluated the orthogonality between ion-pair reversed phase (IP-RP), anion exchange (AEX), and hydrophilic interaction liquid chromatography (HILIC) for the separation of oligonucleotides. The results showed that coupling HILIC with either AEX or IP-RP provided the highest degree of orthogonality.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Petr Kozlik, Jana Vaclova, Kveta Kalikova
Summary: In this study, three recently developed HILIC columns were characterized in detail to compare their peptide retention mechanisms, revealing a multimodal retention mechanism in systems with these stationary phases. By balancing the properties of analytes and stationary phases, satisfactory retention and peak shape of peptides can be achieved. Buffer concentration and pH optimization was confirmed to be an effective tool for manipulating retention and peak symmetry, enhancing the potential applications of these columns in the analysis of diverse compounds.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Sylwia Studzinska, Szymon Bocian, Anna Kilanowska, Boguslaw Buszewski
Summary: This study aimed to develop a new method for separating oligonucleotides by using different mobile phases to improve separation selectivity. The number of anion-exchange layers, type of salt, and pH significantly impacted the analysis of oligonucleotides. The developed chromatographic method exhibited sufficient selectivity for different oligonucleotide lengths. Based on the results, dendrimer stationary phases can be used as alternatives to commonly applied packing materials in ion-exchange chromatography.
Article
Chemistry, Multidisciplinary
Yongyang Song, Xuefang Dong, Danyi Shang, Xiaofei Zhang, Xiuling Li, Xinmiao Liang, Shutao Wang
Summary: This study demonstrates the synthesis of nanofractal microparticles with rapid protein capture, release, and separation capabilities using a strategy of electrostatic interaction regulated emulsion interfacial polymerization. The controlled growth of nanostructures at the microparticle surface enables fast and efficient protein separation.
Article
Engineering, Chemical
A. A. M. Abusultan, J. A. Wood, T. Sainio, A. J. B. Kemperman, W. G. J. van der Meer
Summary: Reverse osmosis (RO) membrane technology is increasingly used for large-scale production of potable water. However, the acidic and unbuffered RO permeate can damage water piping networks. Remineralization using an ion exchange resin - bipolar membrane electrodialysis hybrid process can protect the water distribution network. This study investigates the use of preparative ion exchange chromatography principles for monovalent-divalent ion separation.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Polymer Science
Shihua Dong, Li Yang, Peng Zhang, Hong Wang, Jiaxi Cui
Summary: In this study, a new class of omni-dynamic silicone elastomers was developed via a simple polymerization method. These elastomers exhibited good solubility, excellent mechanical properties, and degradation ability. The unique structure of the elastomers, consisting of an initiator and two catalysts, enabled supramolecular crosslinking and chain exchange reactions.
Article
Chemistry, Analytical
Luyao Liu, Bin Zhu, Zheng Fang, Na Zhang, Hongqiang Qin, Zhimou Guo, Xinmiao Liang, Zhenzhen Yao, Mingliang Ye
Summary: Bottom-up proteomics is increasingly used in clinical research, but glycoproteomic analysis has been a bottleneck. An automated glycopeptide enrichment method was developed for site-specific N-glycoproteome analysis, showing higher efficiency and reliability.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Bastiaan L. Duivelshof, Amarande Murisier, Julien Camperi, Szabolcs Fekete, Alain Beck, Davy Guillarme, Valentina D'Atri
Summary: Fc-fusion proteins are a successful class of biopharmaceutical products with diverse biological ligands, combining tailored pharmacological properties and multiple functions of immunoglobulins. Analyzing Fc-fusion proteins is more complex than monoclonal antibodies due to their highly diverse structures, requiring the development of specific methods for characterization.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Michal Halaj, Maria Matulova, Peter Capek
Summary: Ion-exchange chromatography was used to separate the biologically active extracellular biopolymer produced by D. chlorelloides into ten fractions. These fractions differed in terms of yield, protein content, monosaccharide composition, and molecular weight distribution. The dominant component in all fractions, except one, was rhamnogalactans, which were substituted to different extents by mannose and glucose.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Engineering, Chemical
Xing Yang, Andrea Merenda, Riyadh AL-Attabi, Ludovic F. Dumee, Xiwang Zhang, San H. Thang, Hung Pham, Lingxue Kong
Summary: Membrane chromatography is considered a potential solution for downstream processing in protein purification. Despite limited commercial adoption in large-scale production, significant progress has been made. However, robust evaluation of its performance is still needed, and the disconnection between academic and commercial efforts should be addressed.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Biochemical Research Methods
Yu Isakari, Ales Podgornik, Noriko Yoshimoto, Shuichi Yamamoto
BIOTECHNOLOGY JOURNAL
(2016)
Article
Biochemical Research Methods
Tomokazu Yada, Koichi Nonaka, Masayuki Yabuta, Noriko Yoshimoto, Shuichi Yamamoto
BIOTECHNOLOGY JOURNAL
(2017)
Article
Biochemical Research Methods
Tomokazu Yada, Koichi Nonaka, Masayuki Yabuta, Noriko Yoshimoto, Shuichi Yamamoto
BIOTECHNOLOGY JOURNAL
(2017)
Article
Biochemical Research Methods
Joao C. Simoes-Cardoso, Noriko Yoshimoto, Shuichi Yamamoto
JOURNAL OF CHROMATOGRAPHY A
(2019)
Article
Engineering, Chemical
Sumiko Hasegawa, Chyi-Shin Chen, Noriko Yoshimoto, Shuichi Yamamoto
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
(2020)
Article
Engineering, Chemical
Sumiko Hasegawa, Chyi-Shin Chen, Noriko Yoshimoto, Shuichi Yamamoto
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
(2020)
Article
Biochemical Research Methods
Chyi-Shin Chen, Joao C. Simoes-Cardoso, Yuka Ikezuki, Daichi Tanaka, Noriko Yoshimoto, Shuichi Yamamoto
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Biotechnology & Applied Microbiology
Chyi-Shin Chen, Kosei Ando, Noriko Yoshimoto, Shuichi Yamamoto
Summary: The proposed linear flow-velocity gradient (LFG) method increases productivity and reduces resin cost in capture chromatography. It has great potential for application in optimizing process efficiency.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Engineering, Chemical
Chyi-Shin Chen, Noriko Yoshimoto, Shuichi Yamamoto
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
(2020)
Review
Biochemistry & Molecular Biology
Hidenori Inaba, Kosuke Wakabayashi, Ikuo Tsujimoto, Noriko Yoshimoto, Shuichi Yamamoto
Summary: Recent advancements in cell engineering and bioreactor engineering have led to increased concentrations of monoclonal antibodies (mAbs) in downstream process (DSP). A method for measuring high protein concentration (C-P) using optical rotation (OR) was developed and successfully applied in monitoring C-P in cell culture solutions. The results showed good agreement with the standard Protein A HPLC method, indicating the potential of OR as an efficient process analytical tool (PAT) for DSP.
CURRENT PROTEIN & PEPTIDE SCIENCE
(2021)
Article
Biochemical Research Methods
Lalita Kanwar Shekhawat, Anamika Tiwari, Shuichi Yamamoto, Anurag S. Rathore
Summary: With the increasing demand for therapeutic monoclonal antibodies, in silico downstream process development based on mechanistic modeling of chromatography separation process is becoming popular. However, model calibration, especially obtaining input values for binding kinetic parameters, can be time-consuming. This study proposes an accelerated approach to estimate the input values for binding kinetic parameters using Yamamoto coefficients A and B obtained directly from model calibration linear gradient elution runs. The proposed method is more efficient and accelerated than current approaches, accelerating the development and implementation of mechanistic modeling for process chromatography.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biotechnology & Applied Microbiology
Hironobu Shirataki, Yoshihiro Matsumoto, Fuminori Konoike, Shuichi Yamamoto
Summary: This study proposes an end-to-end integrated continuous process for monoclonal antibody (mAb) production, including upstream production processes, pH-controlled virus inactivation, and flow-through integrated polishing process. The results demonstrate high virus reduction and the potential for continuous production.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Fuminori Konoike, Masatoshi Taniguchi, Shuichi Yamamoto
Summary: A continuous downstream process for monoclonal antibodies was developed using periodic-counter current chromatography (PCCC) for capture, a batch reactor for low pH virus inactivation (VI), and flow-through chromatography (FTC) for polish. Different runs with varying feed rates and concentrations were performed, achieving good recovery and purity values. The process was found to be flexible and stable for feed fluctuations, requiring only a few surge or pool tanks in addition to the final product pool tank.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biochemical Research Methods
Ales Podgornik, Masataka Hamachi, Yu Isakari, Noriko Yoshimoto, Shuichi Yamamoto
Article
Biochemistry & Molecular Biology
Noriko Yoshimoto, Yukiteru Sugiyama, Shuichi Yamamoto
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2017)