Non-conventional expression systems for the production of vaccine proteins and immunotherapeutic molecules

Safety Profile of the 9-Valent HPV Vaccine: A Combined Analysis of 7 Phase III Clinical Trials

(2016) Edson D. Moreira et al.   PEDIATRICS

Yarrowia lipolytica: recent achievements in heterologous protein expression and pathway engineering

(2015) Catherine Madzak   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Lactic acid bacteria—20 years exploring their potential as live vectors for mucosal vaccination

(2015) Agnieszka Wyszyńska et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Biosynthesis of membrane dependent proteins in insect cell lysates: identification of limiting parameters for folding and processing

(2015) Helmut Merk et al.   BIOLOGICAL CHEMISTRY

Cultivation strategies to enhance productivity of Pichia pastoris: A review

(2015) V. Looser et al.   BIOTECHNOLOGY ADVANCES

Expression of the human cytomegalovirus pentamer complex for vaccine use in a CHO system

(2015) Irmgard Hofmann et al.   BIOTECHNOLOGY AND BIOENGINEERING

Designing cell lines for viral vaccine production: Where do we stand?

(2015) Yvonne Genzel   Biotechnology Journal

A simplified and robust protocol for immunoglobulin expression inEscherichia colicell-free protein synthesis systems

(2015) Qi Cai et al.   BIOTECHNOLOGY PROGRESS

Efficient expression of codon-adapted affinity tagged super folder green fluorescent protein for synchronous protein localization and affinity purification studies in Tetrahymena thermophila

(2015) Gürkan Yilmaz et al.   BMC BIOTECHNOLOGY

Development of genetic tools for Myceliophthora thermophila

(2015) Jing Xu et al.   BMC BIOTECHNOLOGY

Exogenous enzymes upgrade transgenesis and genetic engineering of farm animals

(2015) Pablo Bosch et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Stable mammalian producer cell lines for structural biology

(2015) Konrad Büssow   CURRENT OPINION IN STRUCTURAL BIOLOGY

HEK293 in cell biology and cancer research: phenotype, karyotype, tumorigenicity, and stress-induced genome-phenotype evolution

(2015) A.A. Stepanenko et al.   GENE

Alga-Produced Malaria Transmission-Blocking Vaccine Candidate Pfs25 Formulated with a Human Use-Compatible Potent Adjuvant Induces High-Affinity Antibodies That Block Plasmodium falciparum Infection of Mosquitoes

(2015) Kailash P. Patra et al.   INFECTION AND IMMUNITY

Mannose receptor-mediated delivery of moss-made α-galactosidase A efficiently corrects enzyme deficiency in Fabry mice

(2015) Jin-Song Shen et al.   JOURNAL OF INHERITED METABOLIC DISEASE

Expeditious screening of candidate proteins for microbial vaccines

(2015) Rahat Zaheer et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Improving Protein Production on the Level of Regulation of both Expression and Secretion Pathways in Bacillus subtilis

(2015) Yafeng Song et al.   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Potential Antiviral Agents from Marine Fungi: An Overview

(2015) Soheil Moghadamtousi et al.   Marine Drugs

Versatile plasmid-based expression systems for Gram-negative bacteria—General essentials exemplified with the bacterium Ralstonia eutropha H16

(2015) Steffen Gruber et al.   New Biotechnology

Discovery of Novel Plasmodium falciparum Pre-Erythrocytic Antigens for Vaccine Development

(2015) Joao C. Aguiar et al.   PLoS One

Optimisation of a simple method to transiently transfect a CHO cell line in high-throughput and at large scale

(2015) W. Mark Abbott et al.   PROTEIN EXPRESSION AND PURIFICATION

Preclinical development of a dengue tetravalent recombinant subunit vaccine: Immunogenicity and protective efficacy in nonhuman primates

(2015) Dhanasekaran Govindarajan et al.   VACCINE

Immunogenic virus-like particles continuously expressed in mammalian cells as a veterinary rabies vaccine candidate

(2015) Diego Fontana et al.   VACCINE

Designing cell lines for viral vaccine production: Where do we stand?

(2015) Yvonne Genzel   Biotechnology Journal

Leishmania tarentolae: an alternative approach to the production of monoclonal antibodies to treat emerging viral infections

(2015) Joshua D Jones   Infectious Diseases of Poverty

Expression of Recombinant Human Lysozyme in Egg Whites of Transgenic Hens

(2015) Dainan Cao et al.   PLoS One

The Influence of Sub-Unit Composition and Expression System on the Functional Antibody Response in the Development of a VAR2CSA Based Plasmodium falciparum Placental Malaria Vaccine

(2015) Morten A. Nielsen et al.   PLoS One

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

(2014) Mudassar Ahmad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A combined cell-free transcription-translation system fromSaccharomyces cerevisiaefor rapid and robust protein synthe

(2014) Rui Gan et al.   Biotechnology Journal

A review of machine learning methods to predict the solubility of overexpressed recombinant proteins in Escherichia coli

(2014) Narjeskhatoon Habibi et al.   BMC BIOINFORMATICS

Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system

(2014) Matthias Buntru et al.   BMC BIOTECHNOLOGY

Escherichia coli as a glycoprotein production host: recent developments and challenges

(2014) Stephen RP Jaffé et al.   CURRENT OPINION IN BIOTECHNOLOGY

Recombinant protein subunit vaccine synthesis in microbes: a role for yeast?

(2014) Roslyn M. Bill   JOURNAL OF PHARMACY AND PHARMACOLOGY

Engineering toward a bacterial “endoplasmic reticulum” for the rapid expression of immunoglobulin proteins

(2014) Dan Groff et al.   mAbs

Genome engineering for improved recombinant protein expression in Escherichia coli

(2014) Shubhashree Mahalik et al.   Microbial Cell Factories

Biopharmaceutical benchmarks 2014

(2014) Gary Walsh   NATURE BIOTECHNOLOGY

Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses

(2014) Beth A. Rasala et al.   PHOTOSYNTHESIS RESEARCH

A Novel Malaria Vaccine Candidate Antigen Expressed in Tetrahymena thermophila

(2014) Graeme J. M. Cowan et al.   PLoS One

Improved Cell-Free RNA and Protein Synthesis System

(2014) Jun Li et al.   PLoS One

Expression of recombinant human mast cell chymase with Asn-linked glycans in glycoengineered Pichia pastoris

(2014) Eliot T. Smith et al.   PROTEIN EXPRESSION AND PURIFICATION

Characterization and immunogenicity in mice of recombinant influenza haemagglutinins produced in Leishmania tarentolae

(2014) Corinne Pion et al.   VACCINE

The potential of transgenic green microalgae; a robust photobioreactor to produce recombinant therapeutic proteins

(2014) Fariba Akbari et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Plant Virus Expression Vector Development: New Perspectives

(2014) Kathleen Hefferon   Biomed Research International

Algae-based oral recombinant vaccines

(2014) Elizabeth A. Specht et al.   Frontiers in Microbiology

Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis

(2014) Seok Hoon Hong et al.   Frontiers in Chemistry

Preparation of transgenic Dunaliella salina for immunization against white spot syndrome virus in crayfish

(2013) Shuying Feng et al.   ARCHIVES OF VIROLOGY

Cell-free synthesis system suitable for disulfide-containing proteins

(2013) Takayoshi Matsuda et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

A high-yielding CHO transient system: Coexpression of genes encoding EBNA-1 and GS enhances transient protein expression

(2013) Olalekan Daramola et al.   BIOTECHNOLOGY PROGRESS

Cytomegalovirus Vaccines

(2013) Michael A. McVoy   CLINICAL INFECTIOUS DISEASES

Future directions for the development ofChlamydomonas-based vaccines

(2013) Sergio Rosales-Mendoza   Expert Review of Vaccines

Pichia pastoris: protein production host and model organism for biomedical research

(2013) Brigitte Gasser et al.   Future Microbiology

Production of rotavirus core-like particles in Sf9 cells using recombinase-mediated cassette exchange

(2013) Fabiana Fernandes et al.   JOURNAL OF BIOTECHNOLOGY

Purification of hepatitis B surface antigen virus-like particles from recombinant Pichia pastoris and in vivo analysis of their immunogenic properties

(2013) Chandrasekhar Gurramkonda et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Generation and Characterization of a Leishmania tarentolae Strain for Site-Directed in Vivo Biotinylation of Recombinant Proteins

(2013) Stephan Klatt et al.   JOURNAL OF PROTEOME RESEARCH

Expression of a truncated hepatitis E virus capsid protein in the protozoan organism Leishmania tarentolae and its application in a serological assay

(2013) C. Baechlein et al.   JOURNAL OF VIROLOGICAL METHODS

Vaccination of Captive Nēnē (Branta sandvicensis) against West Nile Virus Using a Protein-based Vaccine (WN-80E)

(2013) Susan I. Jarvi et al.   JOURNAL OF WILDLIFE DISEASES

Vaccination against Influenza with Recombinant Hemagglutinin Expressed by Schizochytrium sp. Confers Protective Immunity

(2013) Anne-Cécile V. Bayne et al.   PLoS One

Pichia pastoris-Expressed Dengue 2 Envelope Forms Virus-Like Particles without Pre-Membrane Protein and Induces High Titer Neutralizing Antibodies

(2013) Shailendra Mani et al.   PLoS One

Animal vaccines based on orally presented yeast recombinants

(2013) Min-Kyoung Shin et al.   VACCINE

Expression at a 20L scale and purification of the extracellular domain of theSchistosoma mansoniTSP-2 recombinant protein

(2013) Elena Curti et al.   Human Vaccines & Immunotherapeutics

Expression Systems and Species Used for Transgenic Animal Bioreactors

(2013) Yanli Wang et al.   Biomed Research International

An oral vaccine against candidiasis generated by a yeast molecular display system

(2013) Seiji Shibasaki et al.   Pathogens and Disease

The current state of GMO governance: Are we ready for GM animals?

(2012) Núria Vàzquez-Salat et al.   BIOTECHNOLOGY ADVANCES

Advances in genetic engineering of marine algae

(2012) Song Qin et al.   BIOTECHNOLOGY ADVANCES

Characterization of transferrin receptor-mediated endocytosis and cellular iron delivery of recombinant human serum transferrin from rice (Oryza sativa L.)

(2012) Deshui Zhang et al.   BMC BIOTECHNOLOGY

Expression without boundaries: Cell-free protein synthesis in pharmaceutical research

(2012) Marco G. Casteleijn et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system

(2012) Gang Yin et al.   mAbs

Algae-Produced Pfs25 Elicits Antibodies That Inhibit Malaria Transmission

(2012) James A. Gregory et al.   PLoS One

Protective Vaccination against Infectious Bursal Disease Virus with Whole Recombinant Kluyveromyces lactis Yeast Expressing the Viral VP2 Subunit

(2012) Marina Arnold et al.   PLoS One

Delayed treatment of Ebola virus infection with plant-derived monoclonal antibodies provides protection in rhesus macaques

(2012) G. G. Olinger et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Will GM animals follow the GM plant fate?

(2012) Núria Vàzquez-Salat et al.   TRANSGENIC RESEARCH

Recombinant protein vaccines produced in insect cells

(2012) Manon M.J. Cox   VACCINE

Expression of Recombinant Human Coagulation Factor VII by the LizardLeishmaniaExpression System

(2011) Sina Mirzaahmadi et al.   JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY

Trichoderma reesei RUT-C30 - thirty years of strain improvement

(2011) R. Peterson et al.   MICROBIOLOGY-SGM

Calcium-dependent mitochondrial extrusion in ciliated protozoa

(2011) Yelena Bisharyan et al.   MITOCHONDRION

Reliable protein production in a Pseudomonas fluorescens expression system

(2011) Diane M. Retallack et al.   PROTEIN EXPRESSION AND PURIFICATION

Adding New Chemistries to the Genetic Code

(2010) Chang C. Liu et al.   Annual Review of Biochemistry

Fungal Protein Production: Design and Production of Chimeric Proteins

(2010) Peter J. Punt et al.   Annual Review of Microbiology

Strategies for high-level recombinant protein expression in transgenic microalgae: A review

(2010) Gabriel Potvin et al.   BIOTECHNOLOGY ADVANCES

Efficacy of a Potential Trivalent Vaccine Based on Hc Fragments of Botulinum Toxins A, B, and E Produced in a Cell-Free Expression System

(2010) R. Zichel et al.   Clinical and Vaccine Immunology

Genetic Ablation of Luteinizing Hormone Receptor Improves the Amyloid Pathology in a Mouse Model of Alzheimer Disease

(2010) Jing Lin et al.   JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY

Cell culture processes for monoclonal antibody production

(2010) Feng Li et al.   mAbs

Artificial linear episome-based protein expression system for protozoon Leishmania tarentolae

(2010) Susanna Kushnir et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Lipidation of intact proteins produces highly immunogenic vaccine candidates

(2010) Weiguang Zeng et al.   MOLECULAR IMMUNOLOGY

Pfizer stakes a claim in plant cell–made biopharmaceuticals

(2010) Mark Ratner   NATURE BIOTECHNOLOGY

Practical cell-free protein synthesis system using purified wheat embryos

(2010) Kazuyuki Takai et al.   Nature Protocols

Engineering the Chloroplast Targeted Malarial Vaccine Antigens in Chlamydomonas Starch Granules

(2010) David Dauvillée et al.   PLoS One

Preclinical and Clinical Development of Plant-Made Virus-Like Particle Vaccine against Avian H5N1 Influenza

(2010) Nathalie Landry et al.   PLoS One

Silkworm expression system as a platform technology in life science

(2009) Tatsuya Kato et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Factors effecting expression of vaccines in microalgae

(2009) Raymond Surzycki et al.   BIOLOGICALS

Strategies for analysing and improving the expression and quality of recombinant proteins made in mammalian cells

(2009) Nigel Jenkins et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

GlycoFi's technology to control the glycosylation of recombinant therapeutic proteins

(2009) Alain Beck et al.   Expert Opinion on Drug Discovery

Heat-stable oral alga-based vaccine protects mice from Staphylococcus aureus infection

(2009) Imke A.J. Dreesen et al.   JOURNAL OF BIOTECHNOLOGY

Characterization of Both Polyunsaturated Fatty Acid Biosynthetic Pathways in Schizochytrium sp.

(2009) J. Casey Lippmeier et al.   LIPIDS

Recombinant Protein Expression in Leishmania tarentolae

(2009) Giancarlo Basile et al.   MOLECULAR BIOTECHNOLOGY

Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology

(2009) Pieter P Jacobs et al.   Nature Protocols

Baker's yeast expressing the Japanese encephalitis virus envelope protein on its cell surface: induction of an antigen-specific but non-neutralizing antibody response

(2009) Bhaskar Upadhyaya et al.   YEAST

Developing Aspergillus as a host for heterologous expression

(2008) David Lubertozzi et al.   BIOTECHNOLOGY ADVANCES

An optimized yeast cell-free system: Sufficient for translation of human papillomavirus 58 L1 mRNA and assembly of virus-like particles

(2008) Xiao Wang et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Novel Controlled-Release Lemna-Derived IFN-α2b (Locteron): Pharmacokinetics, Pharmacodynamics, and Tolerability in a Phase I Clinical Trial

(2008) Leo G.J. De Leede et al.   JOURNAL OF INTERFERON AND CYTOKINE RESEARCH

Expression of Foot-and-Mouth Disease Virus Capsid Proteins in Silkworm-Baculovirus Expression System and Its Utilization as a Subunit Vaccine

(2008) Zhiyong Li et al.   PLoS One

Safety and Immunogenicity of H5N1 Influenza Vaccine Based on Baculovirus Surface Display System of Bombyx mori

(2008) Rongzhong Jin et al.   PLoS One

Production of recombinant albumin by a herd of cloned transgenic cattle

(2008) Yann Echelard et al.   TRANSGENIC RESEARCH