Article
Immunology
Manon M. J. Cox
Summary: The insect cell expression system, proposed as the preferred biosecurity strategy for vaccine production, has limitations that need to be addressed for effective use in future pandemics.
Article
Biochemistry & Molecular Biology
Beata Flachner, Krisztina Dobi, Anett Benedek, Sandor Cseh, Zsolt Lorincz, Istvan Hajdu
Summary: This paper describes the expression of RI in baculovirus-infected High Five cells and compares the differences between RI expressed in insect cells and commercially available products. The results show that RI expressed in insect cells has higher thermal stability and is endotoxin-free, making it potentially useful for future therapeutics.
Article
Virology
Hao Zheng, Yong Pan, Xiong Wang, Weibin Tian, Lunguang Yao, Jingchen Sun
Summary: By screening for suitable promoters, utilizing the envelope display effect of INVASIN and VSVG proteins, and constructing a recombinant baculovirus vector, the study was able to improve the efficiency and effectiveness of the baculovirus display system, providing a foundation for further development.
Article
Virology
Huimin Bai, Michiyo Kataoka, Yasushi Ami, Yuriko Suzaki, Naokazu Takeda, Masamichi Muramatsu, Tian-Cheng Li
Summary: Rabbit hepatitis E virus (HEV) is a novel HEV which belongs to genotype 3 in the Orthohepevirus A species. Through expressing truncated rabbit HEV ORF2 proteins, two types of virus-like particles were obtained, with RsVLPs showing stronger antigenic cross-reactivity against different HEV genotypes. Immunization with RsVLPs completely protected rabbits from challenge by rabbit HEV, suggesting potential for vaccine development.
Review
Immunology
Qiaonan Hong, Jian Liu, Yuquan Wei, Xiawei Wei
Summary: Vaccination is an effective strategy to control epidemics, and the baculovirus expression vector system (BEVS) is a promising technology for vaccine production due to its safety and rapid production. This review provides an overview of the development history of BEVS, the procedures for preparing recombinant protein vaccines using the BEVS platform, and the features and limitations of this platform. The progress of BEVS platform-related research, particularly in the field of vaccine, is highlighted, along with a future prospect for BEVS in vaccine manufacturing.
Review
Biochemical Research Methods
Carissa Grose, Zoe Putman, Dominic Esposito
Summary: Despite the success of generating recombinant proteins in insect cells using the Baculovirus Expression Vector System, challenges still exist, such as cytopathic effects, unpredictable co-infection and co-expression, and baculovirus genome instability. A better understanding of viral gene regulation and different promoter options is needed to advance the platform for expressing a greater variety of proteins and complexes in insect cells.
PROTEIN EXPRESSION AND PURIFICATION
(2021)
Article
Biochemical Research Methods
Nikolaus Virgolini, Ryan Hagan, Ricardo Correia, Marco Silvano, Sofia Fernandes, Paula M. Alves, Colin Clarke, Antonio Roldao, Ines A. Isidro
Summary: The insect cell-baculovirus expression vector system (IC-BEVS) is an alternative platform for efficient production of recombinant AAV for gene therapy. Understanding the biological mechanisms of IC-BEVS is crucial for optimizing the system and improving product yield and quality.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Immunology
Mark R. Bruder, Marc G. Aucoin
Summary: The use of CRISPR-Cas9 technology to introduce indel mutations in the baculovirus expression vector system (BEVS) effectively blocked the release of virus particles and improved the production of virus-like particles (VLPs).
Article
Engineering, Chemical
Carina Bannach, Daniel Ruiz Buck, Genna Bobby, Leo P. Graves, Sainan Li, Adam C. Chambers, Elizabeth Gan, Raquel Arinto-Garcia, Robert D. Possee, Linda A. King
Summary: Autographa californica nucleopolyhedrovirus is an efficient expression vector for recombinant proteins in insect cells. The study found that using the AUA codon may slightly improve protein yield, while the AUU codon may lead to leaky translation.
Article
Biotechnology & Applied Microbiology
Yotaro Yamamoto, Hiroyuki Kajiura, Takahiro Nishibu, Kazuhito Fujiyama
Summary: The use of insect cells for the production of recombinant proteins has been a widely employed technique for over 30 years. A newly established highly proliferative insect cell strain, 2g2, has been found to exhibit equivalent productivity and protein bioactivity compared to the traditional Sf9 cell strain. The 2g2 strain also shows potential for baculovirus-free production of the severe acute respiratory syndrome coronavirus 2 spike protein, with comparable or even better productivity and binding activity.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Morikazu Imamura, Naoko Tabeta, Yoshifumi Iwamaru, Hanae Takatsuki, Tsuyoshi Mori, Ryuichiro Atarashi
Summary: This study successfully generated various Bac-rPrP(Sc) variants with different transmissibility and structural properties using a baculovirus-insect cell expression system, and found that they compete with each other and gradually converge in ultrasonic reactions.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Immunology
Keon-Woong Yoon, Ki-Back Chu, Hae-Ji Kang, Min-Ju Kim, Gi-Deok Eom, Jie Mao, Su-Hwa Lee, Md Atique Ahmed, Fu-Shi Quan
Summary: This study found that oral immunization with Toxoplasma gondii vaccines induced a similarly effective immune response as intranasal or intramuscular immunizations, providing a protective effect against lethal infections in mice.
Review
Biotechnology & Applied Microbiology
Minqing Hong, Tingting Li, Wenhui Xue, Sibo Zhang, Lingyan Cui, Hong Wang, Yuyun Zhang, Lizhi Zhou, Ying Gu, Ningshao Xia, Shaowei Li
Summary: BEVS is a widely used system for foreign protein expression, playing a crucial role in vaccine production, gene therapy, and other fields. Despite its advantages, challenges such as unstable expression and reduced protein glycosylation levels remain to be addressed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Genetics & Heredity
Hao Zheng, Yong Pan, Mian Muhammad Awais, Weibin Tian, Jingyang Li, Jingchen Sun
Summary: Apoptosis plays a crucial role in virus-host interactions and insect immune response. The study explores the regulatory mechanisms of virus-induced apoptosis through the expression of apoptotic genes, focusing on the role of inhibitor of apoptosis proteins (IAPs) in group II baculovirus. The results show that BsIAP1 promotes apoptosis in insect cells, while BsIAP2 and BsIAP3 have inhibitory effects. Co-expression of BsIAP2 and BsIAP3 significantly reduces virus-induced apoptosis and increases viral replication. The findings provide insights into the relationship between apoptosis and group II baculovirus IAPs.
Article
Biochemistry & Molecular Biology
Franz-Georg Hanisch
Summary: This article investigates the post-translational modifications of norovirus capsid proteins and reveals glycosylation in certain regions. These modifications may play a role in modulating the immune response. The findings have significant implications for anti-norovirus vaccine design.