期刊
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
卷 25, 期 7, 页码 963-977出版社
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1501.01028
关键词
Promoter; ribosome-binding site; signal peptide; Sec pathway; Tat pathway; ATP-binding cassette transporter
资金
- State Key Development Program for Basic Research of China (973 Program) [2013CB733600]
- National Nature Science Foundation of China [31200036, 31370089, 21446008]
- Key Projects in the Tianjin Science AMP
- Technology Pillar Program [12ZCZDSY12700, 14ZCZDSY00065]
The well-characterized gram-positive bacterium Bacillus subtilis is an outstanding industrial candidate for protein expression owing to its single membrane and high capacity of secretion, simplifying the downstream processing of secretory proteins. During the last few years, there has been continuous progress in the illustration of secretion mechanisms and application of this robust host in various fields of life science, such as enzyme production, feed additives, and food and pharmaceutical industries. Here, we review the developments of Bacillus subtilis as a highly promising expression system illuminating strong chemical- and temperature-inducible and other types of promoters, strategies for ribosome-binding-site utilization, and the novel approach of signal peptide selection. Furthermore, we outline the main steps of the Sec pathway and the relevant elements as well as their interactions. In addition, we introduce the latest discoveries of Tat-related complex structures and functions and the countless applications of this full-folded protein secretion pathway. This review also lists some of the current understandings of ATP-binding cassette transporters. According to the extensive knowledge on the genetic modification strategies and molecular biology of Bacillus subtilis, we propose some suggestions and strategies for improving the yield of intended productions. We expect this to promote striking future developments in the optimization and application of this bacterium.
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