Outsmarting Pathogens with Antibody Engineering

Synergistic Protection against Secondary Pneumococcal Infection by Human Monoclonal Antibodies Targeting Distinct Epitopes

(2023) Aaron D. Gingerich et al.   JOURNAL OF IMMUNOLOGY

Omicron escapes the majority of existing SARS-CoV-2 neutralizing antibodies

(2022) Yunlong Cao et al.   NATURE

An infectious SARS-CoV-2 B.1.1.529 Omicron virus escapes neutralization by therapeutic monoclonal antibodies

(2022) Laura A. VanBlargan et al.   NATURE MEDICINE

Engineered human antibodies for the opsonization and killing of Staphylococcus aureus

(2022) Xinhai Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Improving Antibody Therapeutics by Manipulating the Fc Domain: Immunological and Structural Considerations

(2022) George Delidakis et al.   Annual Review of Biomedical Engineering

Kinetic Studies of the Activation of Bordetella pertussis Adenylate Cyclase by Calmodulin

(2022) Ross L. Stein   BIOCHEMISTRY

Biophysical Characterization and Stability of Modified IgG1 Antibodies with Different Hexamerization Propensities

(2022) Muriel D. van Kampen et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Dromedary camel nanobodies broadly neutralize SARS-CoV-2 variants

(2022) Jessica Hong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Natural Human Immunity Against Staphylococcal Protein A Relies on Effector Functions Triggered by IgG3

(2022) Elena Boero et al.   Frontiers in Immunology

A Molecular Analysis of Memory B Cell and Antibody Responses Against Plasmodium falciparum Merozoite Surface Protein 1 in Children and Adults From Uganda

(2022) S. Jake Gonzales et al.   Frontiers in Immunology

Mosaic RBD nanoparticles protect against challenge by diverse sarbecoviruses in animal models

(2022) Alexander A. Cohen et al.   SCIENCE

Blockade of the Adenylate Cyclase Toxin Synergizes with Opsonizing Antibodies to Protect Mice against Bordetella pertussis

(2022) Andrea M. DiVenere et al.   mBio

Superimmunity by pan-sarbecovirus nanobodies

(2022) Yufei Xiang et al.   Cell Reports

Structural basis for non-canonical integrin engagement by Bordetella adenylate cyclase toxin

(2022) Jory A. Goldsmith et al.   Cell Reports

Neutralizing monoclonal antibodies elicited by mosaic RBD nanoparticles bind conserved sarbecovirus epitopes

(2022) Chengcheng Fan et al.   IMMUNITY

Evolution of the vls Antigenic Variability Locus of the Lyme Disease Pathogen and Development of Recombinant Monoclonal Antibodies Targeting Conserved VlsE Epitopes

(2022) Li Li et al.   Microbiology Spectrum

Antibodies to watch in 2023

(2022) Hélène Kaplon et al.   mAbs

Prospective mapping of viral mutations that escape antibodies used to treat COVID-19

(2021) Tyler N. Starr et al.   SCIENCE

Virtual Screening Approach to Identifying a Novel and Tractable Series of Pseudomonas aeruginosa Elastase Inhibitors

(2021) Simon Leiris et al.   ACS Medicinal Chemistry Letters

Human antibodies targeting a Mycobacterium transporter protein mediate protection against tuberculosis

(2021) Avia Watson et al.   Nature Communications

Efficacy of Margetuximab vs Trastuzumab in Patients With Pretreated ERBB2-Positive Advanced Breast Cancer

(2021) Hope S. Rugo et al.   JAMA Oncology

Pseudomonas aeruginosa elastase (LasB) as a therapeutic target

(2021) Martin J Everett et al.   DRUG DISCOVERY TODAY

Monoclonal antibodies targeting nonstructural viral antigens can activate ADCC against human cytomegalovirus

(2021) Virginia-Maria Vlahava et al.   JOURNAL OF CLINICAL INVESTIGATION

Staphylococcal protein A inhibits complement activation by interfering with IgG hexamer formation

(2021) Ana Rita Cruz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Broadly Reactive Human Monoclonal Antibodies Targeting the Pneumococcal Histidine Triad Protein Protect against Fatal Pneumococcal Infection

(2021) Jiachen Huang et al.   INFECTION AND IMMUNITY

Efficacy and safety of suvratoxumab for prevention of Staphylococcus aureus ventilator-associated pneumonia (SAATELLITE): a multicentre, randomised, double-blind, placebo-controlled, parallel-group, phase 2 pilot trial

(2021) Bruno François et al.   LANCET INFECTIOUS DISEASES

An intracellular nanobody targeting T4SS effector inhibits Ehrlichia infection

(2021) Wenqing Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Human cytomegalovirus antagonizes activation of Fcγ receptors by distinct and synergizing modes of IgG manipulation

(2021) Philipp Kolb et al.   eLife

Monoclonal antibodies opsonise Burkholderia and reduce intracellular actin tail formation in a macrophage infection assay.

(2021) A. Taylor et al.   JOURNAL OF BACTERIOLOGY

SARS-CoV-2 RBD antibodies that maximize breadth and resistance to escape

(2021) Tyler N. Starr et al.   NATURE

Predicting in vivo escape dynamics of HIV-1 from a broadly neutralizing antibody

(2021) Matthijs Meijers et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A protein A based Staphylococcus aureus vaccine with improved safety

(2021) Miaomiao Shi et al.   VACCINE

Potent Killing of Pseudomonas aeruginosa by an Antibody-Antibiotic Conjugate

(2021) Kimberly K. Kajihara et al.   mBio

A Structural Model for the Ligand Binding of Pneumococcal Serotype 3 Capsular Polysaccharide-Specific Protective Antibodies

(2021) Ahmet Ozdilek et al.   mBio

Broad cross-reactivity across sarbecoviruses exhibited by a subset of COVID-19 donor-derived neutralizing antibodies

(2021) Claudia A. Jette et al.   Cell Reports

Structural basis for antibody binding to adenylate cyclase toxin reveals RTX linkers as neutralization-sensitive epitopes

(2021) Jory A. Goldsmith et al.   PLoS Pathogens

Human Single-Chain Antibodies That Neutralize Elastolytic Activity of Pseudomonas aeruginosa LasB

(2021) Sirijan Santajit et al.   Pathogens

Breakthrough therapy designation of nirsevimab for the prevention of lower respiratory tract illness caused by respiratory syncytial virus infections (RSV)

(2021) Harrison C. Bergeron et al.   EXPERT OPINION ON INVESTIGATIONAL DRUGS

Broadly neutralizing antibodies target a haemagglutinin anchor epitope

(2021) Jenna J. Guthmiller et al.   NATURE

SARS-CoV-2 evolution in animals suggests mechanisms for rapid variant selection

(2021) Laura Bashor et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single B cell technologies for monoclonal antibody discovery

(2021) Alessandro Pedrioli et al.   TRENDS IN IMMUNOLOGY

Stabilized coronavirus spike stem elicits a broadly protective antibody

(2021) Ching-Lin Hsieh et al.   Cell Reports

Two Distinct Lysosomal Targeting Strategies Afford Trojan Horse Antibodies With Pan-Filovirus Activity

(2021) Ariel S. Wirchnianski et al.   Frontiers in Immunology

Further Insight into the Mechanism of Human PMN Lysis following Phagocytosis of Staphylococcus aureus

(2021) Viktoria Rungelrath et al.   Microbiology Spectrum

Atomistic Simulations and In Silico Mutational Profiling of Protein Stability and Binding in the SARS-CoV-2 Spike Protein Complexes with Nanobodies: Molecular Determinants of Mutational Escape Mechanisms

(2021) Gennady M. Verkhivker et al.   ACS Omega

A highly conserved cryptic epitope in the receptor binding domains of SARS-CoV-2 and SARS-CoV

(2020) Meng Yuan et al.   SCIENCE

Intracellular neutralisation of rotavirus by VP6-specific IgG

(2020) Sarah L. Caddy et al.   PLoS Pathogens

Protease Inhibition Mechanism of Camelid-like Synthetic Human Antibodies

(2020) Dong Hyun Nam et al.   BIOCHEMISTRY

Four-component Meningococcal Serogroup B Vaccine Induces Antibodies With Bactericidal Activity Against Diverse Outbreak Strains in Adolescents

(2020) Alessia Biolchi et al.   PEDIATRIC INFECTIOUS DISEASE JOURNAL

Antibody-Dependent Enhancement: A Challenge for Developing a Safe Dengue Vaccine

(2020) Rahul Shukla et al.   Frontiers in Cellular and Infection Microbiology

Longitudinal Analysis Reveals Early Development of Three MPER-Directed Neutralizing Antibody Lineages from an HIV-1-Infected Individual

(2019) Shelly J. Krebs et al.   IMMUNITY

Penetration of a Monoclonal Antibody Combination (ASN100) Targeting S. aureus Cytotoxins in Lung Epithelial Lining Fluid: A Randomized, Double-blind, Placebo-controlled, Single Ascending Dose Study in Healthy Volunteers.

(2019) Zoltan Magyarics et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Defining the core essential genome of Pseudomonas aeruginosa

(2019) Bradley E. Poulsen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Novel correlates of protection against pandemic H1N1 influenza A virus infection

(2019) Sophia Ng et al.   NATURE MEDICINE

Multimeric Epitope-Scaffold HIV Vaccines Target V1V2 and Differentially Tune Polyfunctional Antibody Responses

(2019) Ann J. Hessell et al.   Cell Reports

Complement alone drives efficacy of a chimeric antigonococcal monoclonal antibody

(2019) Sunita Gulati et al.   PLOS BIOLOGY

Massive antibody discovery used to probe structure–function relationships of the essential outer membrane protein LptD

(2019) Kelly M Storek et al.   eLife

The Roles of Host and Viral Antibody Fc Receptors in Herpes Simplex Virus (HSV) and Human Cytomegalovirus (HCMV) Infections and Immunity

(2019) Jennifer A. Jenks et al.   Frontiers in Immunology

Structural Basis for Broad HIV-1 Neutralization by the MPER-Specific Human Broadly Neutralizing Antibody LN01

(2019) Dora Pinto et al.   Cell Host & Microbe

High-Throughput Mapping of B Cell Receptor Sequences to Antigen Specificity

(2019) Ian Setliff et al.   CELL

Intracellular delivery of therapeutic antibodies into specific cells using antibody-peptide fusions

(2019) Julie Gaston et al.   Scientific Reports

IgG3 enhances neutralization potency and Fc effector function of an HIV V2-specific broadly neutralizing antibody

(2019) Simone I. Richardson et al.   PLoS Pathogens

Blocking FcRn in humans reduces circulating IgG levels and inhibits IgG immune complex–mediated immune responses

(2019) L. J. Blumberg et al.   Science Advances

Safety and tolerability of a single administration of AR-301, a human monoclonal antibody, in ICU patients with severe pneumonia caused by Staphylococcus aureus: first-in-human trial

(2018) Bruno François et al.   INTENSIVE CARE MEDICINE

Structural basis for antibody targeting of the broadly expressed microbial polysaccharide poly-N-acetylglucosamine

(2018) Caroline Soliman et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Diversion of complement-mediated killing by Bordetella

(2018) Anaïs Thiriard et al.   MICROBES AND INFECTION

A natural human monoclonal antibody targeting Staphylococcus Protein A protects against Staphylococcus aureus bacteremia

(2018) Avanish K. Varshney et al.   PLoS One

Monoclonal antibody targeting the β-barrel assembly machine ofEscherichia coliis bactericidal

(2018) Kelly M. Storek et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A CD4-mimetic compound enhances vaccine efficacy against stringent immunodeficiency virus challenge

(2018) Navid Madani et al.   Nature Communications

How single mutations affect viral escape from broad and narrow antibodies to H1 influenza hemagglutinin

(2018) Michael B. Doud et al.   Nature Communications

Phase 1 study of MEDI3902, an investigational anti–Pseudomonas aeruginosa PcrV and Psl bispecific human monoclonal antibody, in healthy adults

(2018) S.O. Ali et al.   CLINICAL MICROBIOLOGY AND INFECTION

Deep mutational scanning of hemagglutinin helps predict evolutionary fates of human H3N2 influenza variants

(2018) Juhye M. Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Bispecific antibody targets multiple Pseudomonas aeruginosa evasion mechanisms in the lung vasculature

(2017) Ajitha Thanabalasuriar et al.   JOURNAL OF CLINICAL INVESTIGATION

IgG antibodies to dengue enhanced for FcγRIIIA binding determine disease severity

(2017) Taia T. Wang et al.   SCIENCE

A highly potent extended half-life antibody as a potential RSV vaccine surrogate for all infants

(2017) Qing Zhu et al.   Science Translational Medicine

Disarming Staphylococcus aureus from destroying human cells by simultaneously neutralizing six cytotoxins with two human monoclonal antibodies

(2017) Harald Rouha et al.   Virulence

Endotoxin neutralization by an O-antigen specific monoclonal antibody: A potential novel therapeutic approach against Klebsiella pneumoniae ST258

(2017) Valéria Szijártó et al.   Virulence

Invasion of Dendritic Cells, Macrophages and Neutrophils by the Bordetella Adenylate Cyclase Toxin: A Subversive Move to Fool Host Immunity

(2017) Giorgio Fedele et al.   Toxins

A peptide immunization approach to counteract a Staphylococcus aureus protease defense against host immunity

(2016) Robert E. Jordan et al.   IMMUNOLOGY LETTERS

Characterization of Antibody Bipolar Bridging Mediated by the Human Cytomegalovirus Fc Receptor gp68

(2016) Blaise Ndjamen et al.   JOURNAL OF VIROLOGY

Pharmacokinetics and pharmacodynamics of DSTA4637A: A novel THIOMAB™ antibody antibiotic conjugate against Staphylococcus aureus in mice

(2016) Chenguang Zhou et al.   mAbs

Targeted drug delivery through the traceless release of tertiary and heteroaryl amines from antibody–drug conjugates

(2016) Leanna R. Staben et al.   Nature Chemistry

Expression of factor H binding protein in meningococcal strains can vary at least 15-fold and is genetically determined

(2016) Massimiliano Biagini et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A “Trojan horse” bispecific-antibody strategy for broad protection against ebolaviruses

(2016) Anna Z. Wec et al.   SCIENCE

Fc-Binding Ligands of Immunoglobulin G: An Overview of High Affinity Proteins and Peptides

(2016) Weonu Choe et al.   Materials

Lessons from the RV144 Thai Phase III HIV-1 Vaccine Trial and the Search for Correlates of Protection

(2015) Jerome H. Kim et al.   Annual Review of Medicine

Novel antibody–antibiotic conjugate eliminates intracellular S. aureus

(2015) Sophie M. Lehar et al.   NATURE

Prefusion F–specific antibodies determine the magnitude of RSV neutralizing activity in human sera

(2015) Joan O. Ngwuta et al.   Science Translational Medicine

Protein A-neutralizing monoclonal antibody protects neonatal mice against Staphylococcus aureus

(2015) Vilasack Thammavongsa et al.   VACCINE

Broadly Neutralizing Anti-HIV-1 Antibodies Require Fc Effector Functions for In Vivo Activity

(2014) Stylianos Bournazos et al.   CELL

Pertactin-Negative Bordetella pertussis Strains: Evidence for a Possible Selective Advantage

(2014) S. W. Martin et al.   CLINICAL INFECTIOUS DISEASES

Human Anti-IgG1 Hinge Autoantibodies Reconstitute the Effector Functions of Proteolytically Inactivated IgGs

(2014) R. J. Brezski et al.   JOURNAL OF IMMUNOLOGY

The HIV-1 gp120 CD4-Bound Conformation Is Preferentially Targeted by Antibody-Dependent Cellular Cytotoxicity-Mediating Antibodies in Sera from HIV-1-Infected Individuals

(2014) Maxime Veillette et al.   JOURNAL OF VIROLOGY

Five birds, one stone: Neutralization of α-hemolysin and 4 bi-component leukocidins ofStaphylococcus aureuswith a single human monoclonal antibody

(2014) Harald Rouha et al.   mAbs

Developmental pathway for potent V1V2-directed HIV-neutralizing antibodies

(2014) Nicole A. Doria-Rose et al.   NATURE

Broadly neutralizing hemagglutinin stalk–specific antibodies require FcγR interactions for protection against influenza virus in vivo

(2014) David J DiLillo et al.   NATURE MEDICINE

Complement Is Activated by IgG Hexamers Assembled at the Cell Surface

(2014) C. A. Diebolder et al.   SCIENCE

Human Cytomegalovirus Fcγ Binding Proteins gp34 and gp68 Antagonize Fcγ Receptors I, II and III

(2014) Eugenia Corrales-Aguilar et al.   PLoS Pathogens

IgG Subclasses and Allotypes: From Structure to Effector Functions

(2014) Gestur Vidarsson et al.   Frontiers in Immunology

Staphylococcus aureus Elaborates Leukocidin AB To Mediate Escape from within Human Neutrophils

(2013) Ashley L. DuMont et al.   INFECTION AND IMMUNITY

Engineered Protease-resistant Antibodies with Selectable Cell-killing Functions

(2013) Michelle Kinder et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Pseudomonas aeruginosa Utilizes the Type III Secreted Toxin ExoS to Avoid Acidified Compartments within Epithelial Cells

(2013) Susan R. Heimer et al.   PLoS One

Antibody to a conserved antigenic target is protective against diverse prokaryotic and eukaryotic pathogens

(2013) C. Cywes-Bentley et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of RSV Fusion Glycoprotein Trimer Bound to a Prefusion-Specific Neutralizing Antibody

(2013) J. S. McLellan et al.   SCIENCE

Results of a Phase 1 Study of AME-133v (LY2469298), an Fc-Engineered Humanized Monoclonal Anti-CD20 Antibody, in FcγRIIIa-Genotyped Patients with Previously Treated Follicular Lymphoma

(2012) Andres Forero-Torres et al.   CLINICAL CANCER RESEARCH

Protein A-Specific Monoclonal Antibodies and Prevention of Staphylococcus aureus Disease in Mice

(2012) Hwan Keun Kim et al.   INFECTION AND IMMUNITY

Identification of broadly protective human antibodies toPseudomonas aeruginosaexopolysaccharide Psl by phenotypic screening

(2012) Antonio DiGiandomenico et al.   JOURNAL OF EXPERIMENTAL MEDICINE

The in vitro resistance of IgG2 to proteolytic attack concurs with a comparative paucity of autoantibodies against peptide analogs of the IgG2 hinge

(2012) Randall J. Brezski et al.   mAbs

Human Cytomegalovirus Escapes a Naturally Occurring Neutralizing Antibody by Incorporating It into Assembling Virions

(2011) Kate Manley et al.   Cell Host & Microbe

The Sbi Protein Is a Multifunctional Immune Evasion Factor of Staphylococcus aureus

(2011) Emma Jane Smith et al.   INFECTION AND IMMUNITY

Complement-Mediated Bactericidal Activity of Anti-Factor H Binding Protein Monoclonal Antibodies against the Meningococcus Relies upon Blocking Factor H Binding

(2011) Serena Giuntini et al.   INFECTION AND IMMUNITY

Protease inhibitors decrease IgG shedding from Staphylococcus aureus, increasing complement activation and phagocytosis efficiency

(2011) M. F. Fernandez Falcon et al.   JOURNAL OF MEDICAL MICROBIOLOGY

The Herpes Simplex Virus 1 IgG Fc Receptor Blocks Antibody-Mediated Complement Activation and Antibody-Dependent Cellular Cytotoxicity In Vivo

(2011) J. M. Lubinski et al.   JOURNAL OF VIROLOGY

Are bloodstream leukocytes Trojan Horses for the metastasis of Staphylococcus aureus?

(2011) Guy E. Thwaites et al.   NATURE REVIEWS MICROBIOLOGY

Intracellular neutralization of viral infection in polarized epithelial cells by neonatal Fc receptor (FcRn)-mediated IgG transport

(2011) Y. Bai et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Preclinical In Vitro and In Vivo Characterization of the Fully Human Monoclonal IgM Antibody KBPA101 Specific for Pseudomonas aeruginosa Serotype IATS-O11

(2010) M. P. Horn et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Cell cycle arrest induced by the bacterial adenylate cyclase toxins from Bacillus anthracis and Bordetella pertussis

(2010) Mary C. Gray et al.   CELLULAR MICROBIOLOGY

Cleavage of IgGs by proteases associated with invasive diseases

(2010) Randall J. Brezski et al.   mAbs

Antibodies protect against intracellular bacteria by Fc receptor-mediated lysosomal targeting

(2010) N. Joller et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

HIV-1 and influenza antibodies: seeing antigens in new ways

(2009) Peter D Kwong et al.   NATURE IMMUNOLOGY

Tumor-associated and microbial proteases compromise host IgG effector functions by a single cleavage proximal to the hinge

(2009) R. J. Brezski et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Human Cytomegalovirus Fc Receptor gp68 Binds the Fc CH2-CH3 Interface of Immunoglobulin G

(2008) E. R. Sprague et al.   JOURNAL OF VIROLOGY

Microbial complement inhibitors as vaccines

(2008) Seppo Meri et al.   VACCINE

Proteolysis of purified IgGs by human and bacterial enzymes in vitro and the detection of specific proteolytic fragments of endogenous IgG in rheumatoid synovial fluid

(2007) Mary H. Ryan et al.   MOLECULAR IMMUNOLOGY