Estimation of microtubule-generated forces using a DNA origami nanospring

Cross-linkers at growing microtubule ends generate forces that drive actin transport

(2022) Celine Alkemade et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Regulation of microtubule dynamics, mechanics and function through the growing tip

(2021) Nikita B. Gudimchuk et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Mechanisms of Motor-Independent Membrane Remodeling Driven by Dynamic Microtubules

(2020) Ruddi Rodríguez-García et al.   CURRENT BIOLOGY

Individual kinetochore-fibers locally dissipate force to maintain robust mammalian spindle structure

(2020) Alexandra F. Long et al.   JOURNAL OF CELL BIOLOGY

Microneedle manipulation of the mammalian spindle reveals specialized, short-lived reinforcement near chromosomes

(2020) Pooja Suresh et al.   eLife

Microtubules pull the strings: disordered sequences as efficient couplers of microtubule-generated force

(2020) Vladimir A. Volkov   Essays in Biochemistry

The whole is greater than the sum of its parts: at the intersection of order, disorder, and kinetochore function

(2020) Margaux R. Audett et al.   Essays in Biochemistry

A Gradient in Metaphase Tension Leads to a Scaled Cellular Response in Mitosis

(2019) Soumya Mukherjee et al.   DEVELOPMENTAL CELL

Centromere mechanical maturation during mammalian cell mitosis

(2019) Lauren A. Harasymiw et al.   Nature Communications

Mammalian kinetochores count attached microtubules in a sensitive and switch-like manner

(2019) Jonathan Kuhn et al.   JOURNAL OF CELL BIOLOGY

Modulation of Kinesin’s Load-Bearing Capacity by Force Geometry and the Microtubule Track

(2019) Serapion Pyrpassopoulos et al.   BIOPHYSICAL JOURNAL

Molecular determinants of the Ska-Ndc80 interaction and their influence on microtubule tracking and force-coupling

(2019) Pim J Huis in 't Veld et al.   eLife

Force-Induced Unravelling of DNA Origami

(2018) Megan C. Engel et al.   ACS Nano

Multivalency of NDC80 in the outer kinetochore is essential to track shortening microtubules and generate forces

(2018) Vladimir A Volkov et al.   eLife

Measuring NDC80 binding reveals the molecular basis of tension-dependent kinetochore-microtubule attachments

(2018) Tae Yeon Yoo et al.   eLife

A Force-Induced Directional Switch of a Molecular Motor Enables Parallel Microtubule Bundle Formation

(2016) Maxim I. Molodtsov et al.   CELL

A TOG Protein Confers Tension Sensitivity to Kinetochore-Microtubule Attachments

(2016) Matthew P. Miller et al.   CELL

How the kinetochore couples microtubule force and centromere stretch to move chromosomes

(2016) Aussie Suzuki et al.   NATURE CELL BIOLOGY

Chromosome biorientation produces hundreds of piconewtons at a metazoan kinetochore

(2016) Anna A. Ye et al.   Nature Communications

A programmable DNA origami nanospring that reveals force-induced adjacent binding of myosin VI heads

(2016) M. Iwaki et al.   Nature Communications

Molecular basis of outer kinetochore assembly on CENP-T

(2016) Pim J Huis in 't Veld et al.   eLife

Ca2+-independent sortase-A exhibits high selective protein ligation activity in the cytoplasm ofEscherichia coli

(2015) Hidehiko Hirakawa et al.   Biotechnology Journal

Ca2+-independent sortase-A exhibits high selective protein ligation activity in the cytoplasm ofEscherichia coli

(2015) Hidehiko Hirakawa et al.   Biotechnology Journal

How to Measure Molecular Forces in Cells: A Guide to Evaluating Genetically-Encoded FRET-Based Tension Sensors

(2014) Anna-Lena Cost et al.   Cellular and Molecular Bioengineering

SpyAvidin Hubs Enable Precise and Ultrastable Orthogonal Nanoassembly

(2014) Michael Fairhead et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

An improved surface passivation method for single-molecule studies

(2014) Boyang Hua et al.   NATURE METHODS

Computational design of ligand-binding proteins with high affinity and selectivity

(2013) Christine E. Tinberg et al.   NATURE

Long tethers provide high-force coupling of the Dam1 ring to shortening microtubules

(2013) V. A. Volkov et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cortical Dynein Controls Microtubule Dynamics to Generate Pulling Forces that Position Microtubule Asters

(2012) Liedewij Laan et al.   CELL

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

Photo-Cross-Linking-Assisted Thermal Stability of DNA Origami Structures and Its Application for Higher-Temperature Self-Assembly

(2011) Arivazhagan Rajendran et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Tension directly stabilizes reconstituted kinetochore-microtubule attachments

(2010) Bungo Akiyoshi et al.   NATURE

Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics

(2010) Carsten Grashoff et al.   NATURE

A streptavidin variant with slower biotin dissociation and increased mechanostability

(2010) Claire E Chivers et al.   NATURE METHODS

An EB1-Binding Motif Acts as a Microtubule Tip Localization Signal

(2009) Srinivas Honnappa et al.   CELL

Rapid prototyping of 3D DNA-origami shapes with caDNAno

(2009) Shawn M. Douglas et al.   NUCLEIC ACIDS RESEARCH

Sensing Chromosome Bi-Orientation by Spatial Separation of Aurora B Kinase from Kinetochore Substrates

(2009) D. Liu et al.   SCIENCE

Highly inclined thin illumination enables clear single-molecule imaging in cells

(2008) Makio Tokunaga et al.   NATURE METHODS