Molecular determinants of the Ska-Ndc80 interaction and their influence on microtubule tracking and force-coupling

Microtubule end conversion mediated by motors and diffusing proteins with no intrinsic microtubule end-binding activity

(2019) Manas Chakraborty et al.   Nature Communications

Human Ska complex and Ndc80 complex interact to form a load-bearing assembly that strengthens kinetochore–microtubule attachments

(2018) Luke A. Helgeson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Multivalency of NDC80 in the outer kinetochore is essential to track shortening microtubules and generate forces

(2018) Vladimir A Volkov et al.   eLife

Simplified Protocol for Cross-linking Mass Spectrometry Using the MS-Cleavable Cross-linker DSBU with Efficient Cross-link Identification

(2018) Dongqing Pan et al.   ANALYTICAL CHEMISTRY

The kinetochore–microtubule interface at a glance

(2018) Julie K. Monda et al.   JOURNAL OF CELL SCIENCE

A cross-linking/mass spectrometry workflow based on MS-cleavable cross-linkers and the MeroX software for studying protein structures and protein–protein interactions

(2018) Claudio Iacobucci et al.   Nature Protocols

Unique Phylogenetic Distributions of the Ska and Dam1 Complexes Support Functional Analogy and Suggest Multiple Parallel Displacements of Ska by Dam1

(2017) Jolien J. E. van Hooff et al.   Genome Biology and Evolution

Congressing kinetochores progressively load Ska complexes to prevent force-dependent detachment

(2017) Philip Auckland et al.   JOURNAL OF CELL BIOLOGY

Spindle assembly checkpoint satisfaction occurs via end-on but not lateral attachments under tension

(2017) Jonathan Kuhn et al.   JOURNAL OF CELL BIOLOGY

Aurora A kinase phosphorylates Hec1 to regulate metaphase kinetochore–microtubule dynamics

(2017) Keith F. DeLuca et al.   JOURNAL OF CELL BIOLOGY

Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells

(2017) Roshan L. Shrestha et al.   Nature Communications

Phosphatase-regulated recruitment of the spindle- and kinetochore-associated (Ska) complex to kinetochores

(2017) Sushama Sivakumar et al.   Biology Open

A TOG Protein Confers Tension Sensitivity to Kinetochore-Microtubule Attachments

(2016) Matthew P. Miller et al.   CELL

The Ska complex promotes Aurora B activity to ensure chromosome biorientation

(2016) Patrick M. Redli et al.   JOURNAL OF CELL BIOLOGY

Insights from biochemical reconstitution into the architecture of human kinetochores

(2016) John R. Weir et al.   NATURE

biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes

(2016) Florian Weissmann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Conserved Tetramer Junction in the Kinetochore Ndc80 Complex

(2016) Roberto Valverde et al.   Cell Reports

The human SKA complex drives the metaphase-anaphase cell cycle transition by recruiting protein phosphatase 1 to kinetochores

(2016) Sushama Sivakumar et al.   eLife

Molecular basis of outer kinetochore assembly on CENP-T

(2016) Pim J Huis in 't Veld et al.   eLife

Aurora A Kinase Contributes to a Pole-Based Error Correction Pathway

(2015) Anna A. Ye et al.   CURRENT BIOLOGY

Multiple assembly mechanisms anchor the KMN spindle checkpoint platform at human mitotic kinetochores

(2015) Soonjoung Kim et al.   JOURNAL OF CELL BIOLOGY

Building an integrated model of chromosome congression

(2015) P. Auckland et al.   JOURNAL OF CELL SCIENCE

Multisite phosphorylation of the NDC80 complex gradually tunes its microtubule-binding affinity

(2015) Anatoly V. Zaytsev et al.   MOLECULAR BIOLOGY OF THE CELL

A quantitative description of Ndc80 complex linkage to human kinetochores

(2015) Aussie Suzuki et al.   Nature Communications

The Aurora B Kinase in Chromosome Bi-Orientation and Spindle Checkpoint Signaling

(2015) Veronica Krenn et al.   Frontiers in Oncology

Accurate phosphoregulation of kinetochore–microtubule affinity requires unconstrained molecular interactions

(2014) Anatoly V. Zaytsev et al.   JOURNAL OF CELL BIOLOGY

Chromosome congression is promoted by CENP-Q- and CENP-E-dependent pathways

(2014) J. Bancroft et al.   JOURNAL OF CELL SCIENCE

SpyAvidin Hubs Enable Precise and Ultrastable Orthogonal Nanoassembly

(2014) Michael Fairhead et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Kinetochore motors drive congression of peripheral polar chromosomes by overcoming random arm-ejection forces

(2014) Marin Barisic et al.   NATURE CELL BIOLOGY

Structural basis for microtubule recognition by the human kinetochore Ska complex

(2014) Maria Alba Abad et al.   Nature Communications

Conserved and divergent features of kinetochores and spindle microtubule ends from five species

(2013) J. Richard McIntosh et al.   JOURNAL OF CELL BIOLOGY

The Kinetochore-Bound Ska1 Complex Tracks Depolymerizing Microtubules and Binds to Curved Protofilaments

(2012) Jens C. Schmidt et al.   DEVELOPMENTAL CELL

Aurora B controls kinetochore–microtubule attachments by inhibiting Ska complex–KMN network interaction

(2012) Ying Wai Chan et al.   JOURNAL OF CELL BIOLOGY

The Ndc80 internal loop is required for recruitment of the Ska complex to establish end-on microtubule attachment to kinetochores

(2012) Gang Zhang et al.   JOURNAL OF CELL SCIENCE

Structural and Functional Organization of the Ska Complex, a Key Component of the Kinetochore-Microtubule Interface

(2012) A. Arockia Jeyaprakash et al.   MOLECULAR CELL

Recruitment of the human Cdt1 replication licensing protein by the loop domain of Hec1 is required for stable kinetochore–microtubule attachment

(2012) Dileep Varma et al.   NATURE CELL BIOLOGY

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

Multimodal microtubule binding by the Ndc80 kinetochore complex

(2012) Gregory M Alushin et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The Ndc80 kinetochore complex directly modulates microtubule dynamics

(2012) N. T. Umbreit et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Complete Kinetochore Tracking Reveals Error-Prone Homologous Chromosome Biorientation in Mammalian Oocytes

(2011) Tomoya S. Kitajima et al.   CELL

The Spatial Arrangement of Chromosomes during Prometaphase Facilitates Spindle Assembly

(2011) Valentin Magidson et al.   CELL

The Ndc80 Loop Region Facilitates Formation of Kinetochore Attachment to the Dynamic Microtubule Plus End

(2011) Jean-François Maure et al.   CURRENT BIOLOGY

Ndc80 Internal Loop Interacts with Dis1/TOG to Ensure Proper Kinetochore-Spindle Attachment in Fission Yeast

(2011) Kuo-Shun Hsu et al.   CURRENT BIOLOGY

Structural organization of the kinetochore–microtubule interface

(2011) Jennifer G. DeLuca et al.   CURRENT OPINION IN CELL BIOLOGY

Temporal changes in Hec1 phosphorylation control kinetochore-microtubule attachment stability during mitosis

(2011) K. F. DeLuca et al.   JOURNAL OF CELL SCIENCE

The Ndc80 complex uses a tripartite attachment point to couple microtubule depolymerization to chromosome movement

(2011) John G. Tooley et al.   MOLECULAR BIOLOGY OF THE CELL

The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore–microtubule attachment in mitosis

(2011) Lynsie J.R. Sundin et al.   MOLECULAR BIOLOGY OF THE CELL

Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega

(2011) F. Sievers et al.   Molecular Systems Biology

The Dam1 complex confers microtubule plus end–tracking activity to the Ndc80 kinetochore complex

(2010) Fabienne Lampert et al.   JOURNAL OF CELL BIOLOGY

Cooperation of the Dam1 and Ndc80 kinetochore complexes enhances microtubule coupling and is regulated by aurora B

(2010) Jerry F. Tien et al.   JOURNAL OF CELL BIOLOGY

Tension directly stabilizes reconstituted kinetochore-microtubule attachments

(2010) Bungo Akiyoshi et al.   NATURE

The Ndc80 kinetochore complex forms oligomeric arrays along microtubules

(2010) Gregory M. Alushin et al.   NATURE

A streptavidin variant with slower biotin dissociation and increased mechanostability

(2010) Claire E Chivers et al.   NATURE METHODS

Jalview Version 2--a multiple sequence alignment editor and analysis workbench

(2009) A. M. Waterhouse et al.   BIOINFORMATICS

The Ndc80 Kinetochore Complex Forms Load-Bearing Attachments to Dynamic Microtubule Tips via Biased Diffusion

(2009) Andrew F. Powers et al.   CELL

Protein Architecture of the Human Kinetochore Microtubule Attachment Site

(2009) Xiaohu Wan et al.   CELL

Ska3 Is Required for Spindle Checkpoint Silencing and the Maintenance of Chromosome Cohesion in Mitosis

(2009) John R. Daum et al.   CURRENT BIOLOGY

The Human Kinetochore Ska1 Complex Facilitates Microtubule Depolymerization-Coupled Motility

(2009) Julie P.I. Welburn et al.   DEVELOPMENTAL CELL

Stable kinetochore–microtubule interactions depend on the Ska complex and its new component Ska3/C13Orf3

(2009) Thomas N Gaitanos et al.   EMBO JOURNAL

Comparative profiling identifies C13orf3 as a component of the Ska complex required for mammalian cell division

(2009) Mirko Theis et al.   EMBO JOURNAL

Circos: An information aesthetic for comparative genomics

(2009) M. Krzywinski et al.   GENOME RESEARCH

RAMA1 is a novel kinetochore protein involved in kinetochore-microtubule attachment

(2009) J. A. Raaijmakers et al.   JOURNAL OF CELL SCIENCE

Chromosome congression in the absence of kinetochore fibres

(2009) Shang Cai et al.   NATURE CELL BIOLOGY

Separation and detection of large phosphoproteins using Phos-tag SDS-PAGE

(2009) Eiji Kinoshita et al.   Nature Protocols

Implications for Kinetochore-Microtubule Attachment from the Structure of an Engineered Ndc80 Complex

(2008) Claudio Ciferri et al.   CELL

Molecular Basis of Drug Resistance in Aurora Kinases

(2008) Fiona Girdler et al.   CHEMISTRY & BIOLOGY

Kinetochore-Microtubule Attachment Relies on the Disordered N-Terminal Tail Domain of Hec1

(2008) Geoffrey J. Guimaraes et al.   CURRENT BIOLOGY

Kinetochore Attachments Require an Interaction between Unstructured Tails on Microtubules and Ndc80Hec1

(2008) Stephanie A. Miller et al.   CURRENT BIOLOGY

CENP-E combines a slow, processive motor and a flexible coiled coil to produce an essential motile kinetochore tether

(2008) Yumi Kim et al.   JOURNAL OF CELL BIOLOGY

MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification

(2008) Jürgen Cox et al.   NATURE BIOTECHNOLOGY

Whole genome functional analysis identifies novel components required for mitotic spindle integrity in human cells

(2008) Daniel R Rines et al.   GENOME BIOLOGY

Molecular architecture of the kinetochore–microtubule interface

(2007) Iain M. Cheeseman et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY