A cryo-ET survey of microtubules and intracellular compartments in mammalian axons

Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies

(2021) Steffen Klein et al.   Communications Biology

Architecture and mechanism of metazoan retromer:SNX3 tubular coat assembly

(2021) Natalya Leneva et al.   Science Advances

Intercellular Arc Signaling Regulates Vasodilation

(2021) June Bryan de la Peña et al.   JOURNAL OF NEUROSCIENCE

Highly accurate protein structure prediction with AlphaFold

(2021) John Jumper et al.   NATURE

Highly accurate protein structure prediction for the human proteome

(2021) Kathryn Tunyasuvunakool et al.   NATURE

High-resolution asymmetric structure of a Fab–virus complex reveals overlap with the receptor binding site

(2021) Daniel J. Goetschius et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural heterogeneity of cellular K5/K14 filaments as revealed by cryo-electron microscopy

(2021) Miriam S Weber et al.   eLife

In-cell structures of conserved supramolecular protein arrays at the mitochondria–cytoskeleton interface in mammalian sperm

(2021) Miguel Ricardo Leung et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Electron cryo-tomography reveals the subcellular architecture of growing axons in human brain organoids

(2021) Patrick C Hoffmann et al.   eLife

Structures of virus-like capsids formed by the Drosophila neuronal Arc proteins

(2020) Simon Erlendsson et al.   NATURE NEUROSCIENCE

The tubulin code and its role in controlling microtubule properties and functions

(2020) Carsten Janke et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Biochemical reconstitution of branching microtubule nucleation

(2020) Raymundo Alfaro-Aco et al.   eLife

Axonal Endoplasmic Reticulum Dynamics and Its Roles in Neurodegeneration

(2020) Zeynep Öztürk et al.   Frontiers in Neuroscience

Cytoskeletal organization of axons in vertebrates and invertebrates

(2020) Andreas Prokop   JOURNAL OF CELL BIOLOGY

In situ cryo-electron tomography reveals filamentous actin within the microtubule lumen

(2020) Danielle M. Paul et al.   JOURNAL OF CELL BIOLOGY

Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

(2020) Felix R. Wagner et al.   Nature Protocols

Structure of V-ATPase from the mammalian brain

(2020) Yazan M. Abbas et al.   SCIENCE

Charting the native architecture of Chlamydomonas thylakoid membranes with single-molecule precision

(2020) Wojciech Wietrzynski et al.   eLife

MAP6 is an intraluminal protein that induces neuronal microtubules to coil

(2020) Camille Cuveillier et al.   Science Advances

The In Situ Structure of Parkinson’s Disease-Linked LRRK2

(2020) Reika Watanabe et al.   CELL

In-cell architecture of an actively transcribing-translating expressome

(2020) Francis J. O’Reilly et al.   SCIENCE

Cryoelectron Tomography Reveals Nanoscale Organization of the Cytoskeleton and Its Relation to Microtubule Curvature Inside Cells

(2020) Saikat Chakraborty et al.   STRUCTURE

Synaptic Vesicle Precursors and Lysosomes Are Transported by Different Mechanisms in the Axon of Mammalian Neurons

(2020) Raffaella De Pace et al.   Cell Reports

Architecture of the centriole cartwheel‐containing region revealed by cryo‐electron tomography

(2020) Nikolai Klena et al.   EMBO JOURNAL

In-cell architecture of the nuclear pore and snapshots of its turnover

(2020) Matteo Allegretti et al.   NATURE

The molecular structure of mammalian primary cilia revealed by cryo-electron tomography

(2020) Petra Kiesel et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

In situ structure of virus capsids within cell nuclei by correlative light and cryo-electron tomography

(2020) Swetha Vijayakrishnan et al.   Scientific Reports

Feedback-Driven Mechanisms between Microtubules and the Endoplasmic Reticulum Instruct Neuronal Polarity

(2019) Ginny G. Farías et al.   NEURON

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

(2019) Simon Alberti et al.   CELL

Lattice defects induce microtubule self-renewal

(2019) Laura Schaedel et al.   Nature Physics

The Molecular Architecture of Native BBSome Obtained by an Integrated Structural Approach

(2019) Hui-Ting Chou et al.   STRUCTURE

Real-time cryo-electron microscopy data preprocessing with Warp

(2019) Dimitry Tegunov et al.   NATURE METHODS

Structural determinants of microtubule minus end preference in CAMSAP CKK domains

(2019) Joseph Atherton et al.   Nature Communications

Building sensory axons: Delivery and distribution of NaV1.7 channels and effects of inflammatory mediators

(2019) Elizabeth J. Akin et al.   Science Advances

In Situ Structure of an Intact Lipopolysaccharide-Bound Bacterial Surface Layer

(2019) Andriko von Kügelgen et al.   CELL

Direct visualization of degradation microcompartments at the ER membrane

(2019) Sahradha Albert et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Neuronal Gene Arc Encodes a Repurposed Retrotransposon Gag Protein that Mediates Intercellular RNA Transfer

(2018) Elissa D. Pastuzyn et al.   CELL

Retrovirus-like Gag Protein Arc1 Binds RNA and Traffics across Synaptic Boutons

(2018) James Ashley et al.   CELL

Characterization of LAMP1-labeled nondegradative lysosomal and endocytic compartments in neurons

(2018) Xiu-Tang Cheng et al.   JOURNAL OF CELL BIOLOGY

Degradation of dendritic cargos requires Rab7-dependent transport to somatic lysosomes

(2018) Chan Choo Yap et al.   JOURNAL OF CELL BIOLOGY

Microtubules grow by the addition of bent guanosine triphosphate tubulin to the tips of curved protofilaments

(2018) J. Richard McIntosh et al.   JOURNAL OF CELL BIOLOGY

Differentiation and Characterization of Excitatory and Inhibitory Synapses by Cryo-electron Tomography and Correlative Microscopy

(2018) Chang-Lu Tao et al.   JOURNAL OF NEUROSCIENCE

Routine determination of ice thickness for cryo-EM grids

(2018) William J. Rice et al.   JOURNAL OF STRUCTURAL BIOLOGY

Cryo-electron tomography reveals that dynactin recruits a team of dyneins for processive motility

(2018) Danielle A. Grotjahn et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Pleomorphic linkers as ubiquitous structural organizers of vesicles in axons

(2018) Nikolas Schrod et al.   PLoS One

The intriguing nature of dorsal root ganglion neurons: linking structure with polarity and function

(2018) Ana Isabel Nascimento et al.   PROGRESS IN NEUROBIOLOGY

Accumulation of Dense Core Vesicles in Hippocampal Synapses Following Chronic Inactivity

(2018) Chang-Lu Tao et al.   Frontiers in Neuroanatomy

Cryo-electron tomography reveals that dynactin recruits a team of dyneins for processive motility

(2018) Danielle A. Grotjahn et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Microtubule architecture in vitro and in cells revealed by cryo-electron tomography

(2018) Joseph Atherton et al.   Acta Crystallographica Section D-Structural Biology

Cryo-EM shows how dynactin recruits two dyneins for faster movement

(2018) Linas Urnavicius et al.   NATURE

Direct induction of microtubule branching by microtubule nucleation factor SSNA1

(2018) Nirakar Basnet et al.   NATURE CELL BIOLOGY

Severing enzymes amplify microtubule arrays through lattice GTP-tubulin incorporation

(2018) Annapurna Vemu et al.   SCIENCE

The HAUS Complex Is a Key Regulator of Non-centrosomal Microtubule Organization during Neuronal Development

(2018) Inês Cunha-Ferreira et al.   Cell Reports

Morphology of mitochondria in spatially restricted axons revealed by cryo-electron tomography

(2018) Tara D. Fischer et al.   PLOS BIOLOGY

The cryo-EM structure of intraflagellar transport trains reveals how dynein is inactivated to ensure unidirectional anterograde movement in cilia

(2018) Mareike A. Jordan et al.   NATURE CELL BIOLOGY

emClarity: software for high-resolution cryo-electron tomography and subtomogram averaging

(2018) Benjamin A. Himes et al.   NATURE METHODS

Structure and architecture of immature and mature murine leukemia virus capsids

(2018) Kun Qu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Impaired JIP3-dependent axonal lysosome transport promotes amyloid plaque pathology

(2017) Swetha Gowrishankar et al.   JOURNAL OF CELL BIOLOGY

Efficient 3D-CTF correction for cryo-electron tomography using NovaCTF improves subtomogram averaging resolution to 3.4 Å

(2017) Beata Turoňová et al.   JOURNAL OF STRUCTURAL BIOLOGY

Implementation of a cryo-electron tomography tilt-scheme optimized for high resolution subtomogram averaging

(2017) Wim J.H. Hagen et al.   JOURNAL OF STRUCTURAL BIOLOGY

Tubulin acetylation protects long-lived microtubules against mechanical ageing

(2017) Didier Portran et al.   NATURE CELL BIOLOGY

A structural model for microtubule minus-end recognition and protection by CAMSAP proteins

(2017) Joseph Atherton et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Axonal Endoplasmic Reticulum Ca 2+ Content Controls Release Probability in CNS Nerve Terminals

(2017) Jaime de Juan-Sanz et al.   NEURON

MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- versus KIF5-Dependent Cargo Transport in Sensory Neurons

(2017) Laura F. Gumy et al.   NEURON

Large dense-core vesicle exocytosis from mouse dorsal root ganglion neurons is regulated by neuropeptide Y

(2017) Anneka Bost et al.   NEUROSCIENCE

In situ structural studies of tripeptidyl peptidase II (TPPII) reveal spatial association with proteasomes

(2017) Yoshiyuki Fukuda et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Contacts between the endoplasmic reticulum and other membranes in neurons

(2017) Yumei Wu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Intraflagellar Transport and Ciliary Dynamics

(2017) Hiroaki Ishikawa et al.   Cold Spring Harbor Perspectives in Biology

A structural model for microtubule minus-end recognition and protection by CAMSAP proteins

(2017) Joseph Atherton et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Co-option of endogenous viral sequences for host cell function

(2017) John A .Frank et al.   Current Opinion in Virology

Sec61β facilitates the maintenance of endoplasmic reticulum homeostasis by associating microtubules

(2017) Yimeng Zhu et al.   Protein & Cell

9Å structure of the COPI coat reveals that the Arf1 GTPase occupies two contrasting molecular environments

(2017) Svetlana O Dodonova et al.   eLife

3D visualization of mitochondrial solid-phase calcium stores in whole cells

(2017) Sharon Grayer Wolf et al.   eLife

Impaired retrograde transport of axonal autophagosomes contributes to autophagic stress in Alzheimer’s disease neurons

(2017) Prasad Tammineni et al.   eLife

The structure of the COPI coat determined within the cell

(2017) Yury S Bykov et al.   eLife

Kinesin-2 and Apc function at dendrite branch points to resolve microtubule collisions

(2016) Alexis T. Weiner et al.   Cytoskeleton

Self-repair promotes microtubule rescue

(2016) Charlotte Aumeier et al.   NATURE CELL BIOLOGY

EB1 interacts with outwardly curved and straight regions of the microtubule lattice

(2016) Audrey Guesdon et al.   NATURE CELL BIOLOGY

Mechanism of microtubule lumen entry for the α-tubulin acetyltransferase enzyme αTAT1

(2016) Courtney Coombes et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Prevalent presence of periodic actin–spectrin-based membrane skeleton in a broad range of neuronal cell types and animal species

(2016) Jiang He et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Visualizing the molecular sociology at the HeLa cell nuclear periphery

(2016) J. Mahamid et al.   SCIENCE

Intermediate Filaments: Structure and Assembly

(2016) Harald Herrmann et al.   Cold Spring Harbor Perspectives in Biology

Corrigendum: Flux of signalling endosomes undergoing axonal retrograde transport is encoded by presynaptic activity and TrkB

(2016) Tong Wang et al.   Nature Communications

Non-centrosomal nucleation mediated by augmin organizes microtubules in post-mitotic neurons and controls axonal microtubule polarity

(2016) Carlos Sánchez-Huertas et al.   Nature Communications

A dynamic formin-dependent deep F-actin network in axons

(2015) Archan Ganguly et al.   JOURNAL OF CELL BIOLOGY

Microtubules self-repair in response to mechanical stress

(2015) Laura Schaedel et al.   NATURE MATERIALS

Control of microtubule organization and dynamics: two ends in the limelight

(2015) Anna Akhmanova et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Building the Neuronal Microtubule Cytoskeleton

(2015) Lukas C. Kapitein et al.   NEURON

Three-dimensional architecture of extended synaptotagmin-mediated endoplasmic reticulum–plasma membrane contact sites

(2015) Rubén Fernández-Busnadiego et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The structure of the dynactin complex and its interaction with dynein

(2015) L. Urnavicius et al.   SCIENCE

Direct observation shows superposition and large scale flexibility within cytoplasmic dynein motors moving along microtubules

(2015) Hiroshi Imai et al.   Nature Communications

Molecular Basis for Age-Dependent Microtubule Acetylation by Tubulin Acetyltransferase

(2014) Agnieszka Szyk et al.   CELL

Autophagosome Biogenesis in Primary Neurons Follows an Ordered and Spatially Regulated Pathway

(2014) Sandra Maday et al.   DEVELOPMENTAL CELL

A Cluster of Thin Tubular Structures Mediates Transformation of the Endoplasmic Reticulum to Autophagic Isolation Membrane

(2014) T. Uemura et al.   MOLECULAR AND CELLULAR BIOLOGY

Structure of human cytoplasmic dynein-2 primed for its power stroke

(2014) Helgo Schmidt et al.   NATURE

The Complex Ultrastructure of the Endolysosomal System

(2014) J. Klumperman et al.   Cold Spring Harbor Perspectives in Biology

An assay to image neuronal microtubule dynamics in mice

(2014) Tatjana Kleele et al.   Nature Communications

Spectrins: A structural platform for stabilization and activation of membrane channels, receptors and transporters

(2013) Beata Machnicka et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Transglutaminase and Polyamination of Tubulin: Posttranslational Modification for Stabilizing Axonal Microtubules

(2013) Yuyu Song et al.   NEURON

Turning gold into ‘junk’: transposable elements utilize central proteins of cellular networks

(2013) György Abrusán et al.   NUCLEIC ACIDS RESEARCH

EBs Recognize a Nucleotide-Dependent Structural Cap at Growing Microtubule Ends

(2012) Sebastian P. Maurer et al.   CELL

Posttranslational Acetylation of α-Tubulin Constrains Protofilament Number in Native Microtubules

(2012) Juan G. Cueva et al.   CURRENT BIOLOGY

Doublecortin Recognizes the 13-Protofilament Microtubule Cooperatively and Tracks Microtubule Ends

(2012) Susanne Bechstedt et al.   DEVELOPMENTAL CELL

MAP6-F Is a Temperature Sensor That Directly Binds to and Protects Microtubules from Cold-induced Depolymerization

(2012) Christian Delphin et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Autophagosomes initiate distally and mature during transport toward the cell soma in primary neurons

(2012) Sandra Maday et al.   JOURNAL OF CELL BIOLOGY

Electron tomographic analysis of synaptic ultrastructure

(2012) Alain C. Burette et al.   JOURNAL OF COMPARATIVE NEUROLOGY

Direct Determination of Actin Polarity in the Cell

(2012) Akihiro Narita et al.   JOURNAL OF MOLECULAR BIOLOGY

Dynamo: A flexible, user-friendly development tool for subtomogram averaging of cryo-EM data in high-performance computing environments

(2012) Daniel Castaño-Díez et al.   JOURNAL OF STRUCTURAL BIOLOGY

RELION: Implementation of a Bayesian approach to cryo-EM structure determination

(2012) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

Rab10 GTPase regulates ER dynamics and morphology

(2012) Amber R. English et al.   NATURE CELL BIOLOGY

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

Endogenous viruses: insights into viral evolution and impact on host biology

(2012) Cédric Feschotte et al.   NATURE REVIEWS GENETICS

Actin, Spectrin, and Associated Proteins Form a Periodic Cytoskeletal Structure in Axons

(2012) K. Xu et al.   SCIENCE

Transport of receptors, receptor signaling complexes and ion channels via neuropeptide-secretory vesicles

(2011) Bo Zhao et al.   CELL RESEARCH

Electron tomography reveals a flared morphology on growing microtubule ends

(2011) J. L. Hoog et al.   JOURNAL OF CELL SCIENCE

Macromolecular organization of ATP synthase and complex I in whole mitochondria

(2011) K. M. Davies et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Multivesicular bodies in neurons: Distribution, protein content, and trafficking functions

(2011) Christopher S. Von Bartheld et al.   PROGRESS IN NEUROBIOLOGY

Crystal structure and functional interpretation of the erythrocyte spectrin tetramerization domain complex

(2010) J. J. Ipsaro et al.   BLOOD

Hereditary spastic paraplegia proteins REEP1, spastin, and atlastin-1 coordinate microtubule interactions with the tubular ER network

(2010) Seong H. Park et al.   JOURNAL OF CLINICAL INVESTIGATION

CTF determination and correction for low dose tomographic tilt series

(2009) Quanren Xiong et al.   JOURNAL OF STRUCTURAL BIOLOGY

The Structure of Rat Liver Vault at 3.5 Angstrom Resolution

(2009) H. Tanaka et al.   SCIENCE

Vaults and the major vault protein: Novel roles in signal pathway regulation and immunity

(2008) W. Berger et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Microtubule stabilization specifies initial neuronal polarization

(2008) Harald Witte et al.   JOURNAL OF CELL BIOLOGY

Imaging of evoked dense-core-vesicle exocytosis in hippocampal neurons reveals long latencies and kiss-and-run fusion events

(2008) X. Xia et al.   JOURNAL OF CELL SCIENCE

Protein 600 Is a Microtubule/Endoplasmic Reticulum-Associated Protein in CNS Neurons

(2008) S. Y. Shim et al.   JOURNAL OF NEUROSCIENCE

Neuronal intermediate filament expression in rat dorsal root ganglia sensory neurons: An in vivo and in vitro study

(2008) M. Fornaro et al.   NEUROSCIENCE

Cryo electron tomography of vitrified fibroblasts: Microtubule plus ends in situ

(2007) Roman I. Koning et al.   JOURNAL OF STRUCTURAL BIOLOGY

Identification of an Axonal Kinesin-3 Motor for Fast Anterograde Vesicle Transport that Facilitates Retrograde Transport of Neuropeptides

(2007) Rosemarie V. Barkus et al.   MOLECULAR BIOLOGY OF THE CELL