Heritable human genome editing: Research progress, ethical considerations, and hurdles to clinical practice

Ethics in embryo research: a position statement by the ASRM Ethics in Embryo Research Task Force and the ASRM Ethics Committee

(2020) Paula Amato et al.   FERTILITY AND STERILITY

Therapeutic Germline Editing: Sense and Sensibility

(2020) Eli Y. Adashi et al.   TRENDS IN GENETICS

Efficient SNP editing in haploid human pluripotent stem cells

(2020) Lauren Zakarin Safier et al.   JOURNAL OF ASSISTED REPRODUCTION AND GENETICS

The mutational impact of culturing human pluripotent and adult stem cells

(2020) Ewart Kuijk et al.   Nature Communications

Efficient generation of mouse models with the prime editing system

(2020) Yao Liu et al.   Cell Discovery

Mapping Gene Circuits Essential for Germ Layer Differentiation via Loss-of-Function Screens in Haploid Human Embryonic Stem Cells

(2020) Atilgan Yilmaz et al.   Cell Stem Cell

A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing

(2020) Beverly Y. Mok et al.   NATURE

Gene Editing Sperm and Eggs (not Embryos): Does it Make a Legal or Ethical Difference?

(2020) I. Glenn Cohen et al.   JOURNAL OF LAW MEDICINE & ETHICS

Human Germ Line and Heritable Genome Editing: The Global Policy Landscape

(2020) Françoise Baylis et al.   CRISPR Journal

Allele-Specific Chromosome Removal after Cas9 Cleavage in Human Embryos

(2020) Michael V. Zuccaro et al.   CELL

How much should having a baby cost?

(2020) Sarah DeWeerdt   NATURE

Reconstitution of the oocyte transcriptional network with transcription factors

(2020) Nobuhiko Hamazaki et al.   NATURE

Delivery technologies for in utero gene therapy

(2020) Rohan Palanki et al.   ADVANCED DRUG DELIVERY REVIEWS

Adopt a moratorium on heritable genome editing

(2019) Eric S. Lander et al.   NATURE

Moral reasons to edit the human genome: picking up from the Nuffield report

(2019) Christopher Gyngell et al.   JOURNAL OF MEDICAL ETHICS

An ethical pathway for gene editing

(2019) Julian Savulescu et al.   BIOETHICS

Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant

(2019) Tarun S. Nambiar et al.   Nature Communications

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

In vitro expansion of human sperm through nuclear transfer

(2019) Xiaoyu Merlin Zhang et al.   CELL RESEARCH

Human germline genome editing

(2019) Rebecca A. Lea et al.   NATURE CELL BIOLOGY

Responsible innovation in human germline gene editing: Background document to the recommendations of ESHG and ESHRE

(2018) Guido De Wert et al.   EUROPEAN JOURNAL OF HUMAN GENETICS

Use of preimplantation genetic testing for monogenic defects (PGT-M) for adult-onset conditions: an Ethics Committee opinion

(2018) Judith Daar et al.   FERTILITY AND STERILITY

Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos

(2018) Bin Gu et al.   NATURE BIOTECHNOLOGY

Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements

(2018) Michael Kosicki et al.   NATURE BIOTECHNOLOGY

Progress in mitochondrial replacement therapies

(2018) Mary Herbert et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Gene editing in human development: ethical concerns and practical applications

(2018) Janet Rossant   DEVELOPMENT

Correction of the Marfan Syndrome Pathogenic FBN1 Mutation by Base Editing in Human Cells and Heterozygous Embryos

(2018) Yanting Zeng et al.   MOLECULAR THERAPY

Ma et al. reply

(2018) Hong Ma et al.   NATURE

Large deletions induced by Cas9 cleavage

(2018) Fatwa Adikusuma et al.   NATURE

Generation of human oogonia from induced pluripotent stem cells in vitro

(2018) Chika Yamashiro et al.   SCIENCE

Genome-edited baby claim provokes international outcry

(2018) David Cyranoski et al.   NATURE

Base editing: precision chemistry on the genome and transcriptome of living cells

(2018) Holly A. Rees et al.   NATURE REVIEWS GENETICS

Treatment of heritable diseases using CRISPR: Hopes, fears, and reality

(2018) Natalie Kofler et al.   SEMINARS IN PERINATOLOGY

Genetic diseases and aneuploidies can be detected with a single blastocyst biopsy: a successful clinical approach

(2017) Maria Giulia Minasi et al.   HUMAN REPRODUCTION

ESHRE PGD Consortium data collection XIV–XV: cycles from January 2011 to December 2012 with pregnancy follow-up to October 2013†

(2017) M De Rycke et al.   HUMAN REPRODUCTION

CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein

(2017) Lichun Tang et al.   MOLECULAR GENETICS AND GENOMICS

Targeted Deletion of an Entire Chromosome Using CRISPR/Cas9

(2017) Fatwa Adikusuma et al.   MOLECULAR THERAPY

Correction of a pathogenic gene mutation in human embryos

(2017) Hong Ma et al.   NATURE

Genome editing reveals a role for OCT4 in human embryogenesis

(2017) Norah M. E. Fogarty et al.   NATURE

Inhibition of 53BP1 favors homology-dependent DNA repair and increases CRISPR–Cas9 genome-editing efficiency

(2017) Marella D Canny et al.   NATURE BIOTECHNOLOGY

Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions

(2017) Y Bill Kim et al.   NATURE BIOTECHNOLOGY

Anti-CRISPR: discovery, mechanism and function

(2017) April Pawluk et al.   NATURE REVIEWS MICROBIOLOGY

Live birth derived from oocyte spindle transfer to prevent mitochondrial disease

(2017) John Zhang et al.   REPRODUCTIVE BIOMEDICINE ONLINE

Hotspots of De Novo Point Mutations in Induced Pluripotent Stem Cells

(2017) Masahito Yoshihara et al.   Cell Reports

Highly efficient base editing in human tripronuclear zygotes

(2017) Changyang Zhou et al.   Protein & Cell

Highly efficient and precise base editing in discarded human tripronuclear embryos

(2017) Guanglei Li et al.   Protein & Cell

Correction of β-thalassemia mutant by base editor in human embryos

(2017) Puping Liang et al.   Protein & Cell

Disabling Cas9 by an anti-CRISPR DNA mimic

(2017) Jiyung Shin et al.   Science Advances

Introducing precise genetic modifications into human 3PN embryos by CRISPR/Cas-mediated genome editing

(2016) Xiangjin Kang et al.   JOURNAL OF ASSISTED REPRODUCTION AND GENETICS

Derivation and differentiation of haploid human embryonic stem cells

(2016) Ido Sagi et al.   NATURE

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

Finding an ethical path forward for mitochondrial replacement

(2016) A. B. Claiborne et al.   SCIENCE

Whole-genome mutational burden analysis of three pluripotency induction methods

(2016) Kunal Bhutani et al.   Nature Communications

Mutational History of a Human Cell Lineage from Somatic to Induced Pluripotent Stem Cells

(2016) Foad J. Rouhani et al.   PLoS Genetics

Germline Modification and the Burden of Human Existence

(2015) JOHN HARRIS   CAMBRIDGE QUARTERLY OF HEALTHCARE ETHICS

Selective Elimination of Mitochondrial Mutations in the Germline by Genome Editing

(2015) Pradeep Reddy et al.   CELL

Don’t edit the human germ line

(2015) Edward Lanphier et al.   NATURE

Evidence of Selection against Complex Mitotic-Origin Aneuploidy during Preimplantation Development

(2015) Rajiv C. McCoy et al.   PLoS Genetics

CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes

(2015) Puping Liang et al.   Protein & Cell

Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells

(2014) Yuxuan Wu et al.   CELL RESEARCH

Aneuploidy across individual chromosomes at the embryonic level in trophectoderm biopsies: changes with patient age and chromosome structure

(2014) Jason M. Franasiak et al.   JOURNAL OF ASSISTED REPRODUCTION AND GENETICS

Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients

(2013) Gerald Schwank et al.   Cell Stem Cell

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Repair of Strand Breaks by Homologous Recombination

(2013) M. Jasin et al.   Cold Spring Harbor Perspectives in Biology

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Offspring from Oocytes Derived from in Vitro Primordial Germ Cell-like Cells in Mice

(2012) K. Hayashi et al.   SCIENCE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

A Systematic Survey of Loss-of-Function Variants in Human Protein-Coding Genes

(2012) D. G. MacArthur et al.   SCIENCE

Reconstitution of the Mouse Germ Cell Specification Pathway in Culture by Pluripotent Stem Cells

(2011) Katsuhiko Hayashi et al.   CELL

Somatic coding mutations in human induced pluripotent stem cells

(2011) Athurva Gore et al.   NATURE

53BP1 Inhibits Homologous Recombination in Brca1-Deficient Cells by Blocking Resection of DNA Breaks

(2010) Samuel F. Bunting et al.   CELL

A cytogenetic survey of 14,069 newborn infants

(2010) J. L. Hamerton et al.   CLINICAL GENETICS