A Roadmap for the Molecular Farming of Viral Glycoprotein Vaccines: Engineering Glycosylation and Glycosylation-Directed Folding

Co‐expression of human calreticulin significantly improves the production of HIV gp140 and other viral glycoproteins in plants

(2020) E Margolin et al.   PLANT BIOTECHNOLOGY JOURNAL

Discovery of O-Linked Carbohydrate on HIV-1 Envelope and Its Role in Shielding against One Category of Broadly Neutralizing Antibodies

(2020) Zachary A. Silver et al.   Cell Reports

Engineering the Plant Secretory Pathway for the Production of Next-Generation Pharmaceuticals

(2020) Emmanuel A. Margolin et al.   TRENDS IN BIOTECHNOLOGY

Structure-Based Design of Prefusion-Stabilized Filovirus Glycoprotein Trimers

(2020) Lucy Rutten et al.   Cell Reports

Efficient N-Glycosylation of the Heavy Chain Tailpiece Promotes the Formation of Plant-Produced Dimeric IgA

(2020) Kathrin Göritzer et al.   Frontiers in Chemistry

Iminosugars: a host-targeted approach to combat Flaviviridae infections

(2020) Lisa Evans DeWald et al.   ANTIVIRAL RESEARCH

Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor

(2020) Peng Zhao et al.   Cell Host & Microbe

Exploitation of glycosylation in enveloped virus pathobiology

(2019) Yasunori Watanabe et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Protein Quality Control in the Endoplasmic Reticulum

(2019) Benjamin M. Adams et al.   PROTEIN JOURNAL

Engineering the interactions between a plant‐produced HIV antibody and human Fc receptors

(2019) Szymon Stelter et al.   PLANT BIOTECHNOLOGY JOURNAL

In vitro and in vivo efficacy of anti-chikungunya virus monoclonal antibodies produced in wild-type and glycoengineered Nicotiana benthamiana plants

(2019) Jonathan Hurtado et al.   PLANT BIOTECHNOLOGY JOURNAL

Immunogenicity and safety of a quadrivalent plant-derived virus like particle influenza vaccine candidate—Two randomized Phase II clinical trials in 18 to 49 and ≥50 years old adults

(2019) Stéphane Pillet et al.   PLoS One

In Planta Glycan Engineering and Functional Activities of IgE Antibodies

(2019) Laura Montero-Morales et al.   Frontiers in Bioengineering and Biotechnology

Multiple roles for HIV broadly neutralizing antibodies

(2019) Barton F. Haynes et al.   Science Translational Medicine

Production and Immunogenicity of Soluble Plant-Produced HIV-1 Subtype C Envelope gp140 Immunogens

(2019) Emmanuel Margolin et al.   Frontiers in Plant Science

Molecular Pharming for low and middle income countries

(2019) Sheeba Murad et al.   CURRENT OPINION IN BIOTECHNOLOGY

Cryo–electron microscopy structures of human oligosaccharyltransferase complexes OST-A and OST-B

(2019) Ana S. Ramírez et al.   SCIENCE

Structure and Immune Recognition of the HIV Glycan Shield

(2018) Max Crispin et al.   Annual Review of Biophysics

Protein Quality Control in the Endoplasmic Reticulum of Plants

(2018) Richard Strasser   Annual Review of Plant Biology

Harnessing post-translational modifications for next-generation HIV immunogens

(2018) Joel D. Allen et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Common Features of Enveloped Viruses and Implications for Immunogen Design for Next-Generation Vaccines

(2018) Felix A. Rey et al.   CELL

CRISPR/Cas9-mediated knockout of six glycosyltransferase genes in Nicotiana benthamiana for the production of recombinant proteins lacking β-1,2-xylose and core α-1,3-fucose

(2018) Julia Jansing et al.   PLANT BIOTECHNOLOGY JOURNAL

Plant-made E2 glycoprotein single-dose vaccine protects pigs against classical swine fever

(2018) Richard C. Laughlin et al.   PLANT BIOTECHNOLOGY JOURNAL

An oligosaccharyltransferase from Leishmania major increases the N-glycan occupancy on recombinant glycoproteins produced in Nicotiana benthamiana

(2018) Alexandra Castilho et al.   PLANT BIOTECHNOLOGY JOURNAL

Production of complex viral glycoproteins in plants as vaccine immunogens

(2018) Emmanuel Margolin et al.   PLANT BIOTECHNOLOGY JOURNAL

Structure of the Lassa virus glycan shield provides a model for immunological resistance

(2018) Yasunori Watanabe et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of a cleavage-independent HIV Env recapitulates the glycoprotein architecture of the native cleaved trimer

(2018) Anita Sarkar et al.   Nature Communications

Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer

(2018) Liwei Cao et al.   Nature Communications

Site-Specific Glycosylation of Virion-Derived HIV-1 Env Is Mimicked by a Soluble Trimeric Immunogen

(2018) Weston B. Struwe et al.   Cell Reports

Chimaeric Rift Valley Fever Virus-Like Particle Vaccine Candidate Production in Nicotiana benthamiana

(2018) Sandiswa Mbewana et al.   Biotechnology Journal

Recent progress in broadly neutralizing antibodies to HIV

(2018) Devin Sok et al.   NATURE IMMUNOLOGY

Innate immune recognition of glycans targets HIV nanoparticle immunogens to germinal centers

(2018) Talar Tokatlian et al.   SCIENCE

Glycosylation profiling to evaluate glycoprotein immunogens against HIV-1

(2017) Anna-Janina Behrens et al.   Expert Review of Proteomics

Native-like Env trimers as a platform for HIV-1 vaccine design

(2017) Rogier W. Sanders et al.   IMMUNOLOGICAL REVIEWS

A Perspective on the Structural and Functional Constraints for Immune Evasion: Insights from Influenza Virus

(2017) Nicholas C. Wu et al.   JOURNAL OF MOLECULAR BIOLOGY

Residues Comprising the Enhanced Aromatic Sequon Influence Protein N-Glycosylation Efficiency

(2017) Yen-Wen Huang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Immunogenicity of plant-produced African horse sickness virus-like particles: implications for a novel vaccine

(2017) Susan J. Dennis et al.   PLANT BIOTECHNOLOGY JOURNAL

Plant-produced Zika virus envelope protein elicits neutralizing immune responses that correlate with protective immunity against Zika virus in mice

(2017) Ming Yang et al.   PLANT BIOTECHNOLOGY JOURNAL

Interdomain conformational flexibility underpins the activity of UGGT, the eukaryotic glycoprotein secretion checkpoint

(2017) Pietro Roversi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Comparison of N - and O -linked glycosylation patterns of ebolavirus glycoproteins

(2017) Amanda L. Collar et al.   VIROLOGY

A plant-derived quadrivalent virus like particle influenza vaccine induces cross-reactive antibody and T cell response in healthy adults

(2016) Stéphane Pillet et al.   CLINICAL IMMUNOLOGY

Iminosugars: Promising therapeutics for influenza infection

(2016) Beatrice Ellen Tyrrell et al.   CRITICAL REVIEWS IN MICROBIOLOGY

Protein quality control at the endoplasmic reticulum

(2016) K. McCaffrey et al.   Essays in Biochemistry

Plant protein glycosylation

(2016) Richard Strasser   GLYCOBIOLOGY

Mutational and functional analysis of N-linked glycosylation of envelope fusion protein F of Helicoverpa armigera nucleopolyhedrovirus

(2016) Shu Shen et al.   JOURNAL OF GENERAL VIROLOGY

Reduced paucimannosidicN-glycan formation by suppression of a specific β-hexosaminidase fromNicotiana benthamiana

(2016) Yun-Ji Shin et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineering of complex protein sialylation in plants

(2016) Somanath Kallolimath et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Monoclonal antibody therapy for Junin virus infection

(2016) Larry Zeitlin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Plant-produced biopharmaceuticals: A case of technical developments driving clinical deployment

(2016) G. P. Lomonossoff et al.   SCIENCE

Transient Glyco-Engineering to Produce Recombinant IgA1 with Defined N- and O-Glycans in Plants

(2016) Martina Dicker et al.   Frontiers in Plant Science

Enhanced Aromatic Sequons Increase Oligosaccharyltransferase Glycosylation Efficiency and Glycan Homogeneity

(2015) Amber N. Murray et al.   CHEMISTRY & BIOLOGY

A sweet code for glycoprotein folding

(2015) Julio J. Caramelo et al.   FEBS LETTERS

Single-Chain Soluble BG505.SOSIP gp140 Trimers as Structural and Antigenic Mimics of Mature Closed HIV-1 Env

(2015) Ivelin S. Georgiev et al.   JOURNAL OF VIROLOGY

Human Lectins and Their Roles in Viral Infections

(2015) Christopher Mason et al.   MOLECULES

Plant-produced candidate countermeasures against emerging and reemerging infections and bioterror agents

(2015) Stephen J. Streatfield et al.   PLANT BIOTECHNOLOGY JOURNAL

Commercial-scale biotherapeutics manufacturing facility for plant-made pharmaceuticals

(2015) Barry R. Holtz et al.   PLANT BIOTECHNOLOGY JOURNAL

Characterization of plants expressing the human β1,4-galactosyltrasferase gene

(2015) Jeannine Schneider et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

An intrinsic mechanism of secreted protein aging and turnover

(2015) Won Ho Yang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Crystal structure of a fully glycosylated HIV-1 gp120 core reveals a stabilizing role for the glycan at Asn262

(2015) Leopold Kong et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Plant-derived H7 VLP vaccine elicits protective immune response against H7N9 influenza virus in mice and ferrets

(2015) S. Pillet et al.   VACCINE

Contribution of N-linked glycans on HSV-2 gB to cell–cell fusion and viral entry

(2015) Sukun Luo et al.   VIROLOGY

N-Glycosylation of cholera toxin B subunit in Nicotiana benthamiana: impacts on host stress response, production yield and vaccine potential

(2015) Krystal Teasley Hamorsky et al.   Scientific Reports

Cleavage-Independent HIV-1 Env Trimers Engineered as Soluble Native Spike Mimetics for Vaccine Design

(2015) Shailendra Kumar Sharma et al.   Cell Reports

Protective mAbs and Cross-Reactive mAbs Raised by Immunization with Engineered Marburg Virus GPs

(2015) Marnie L. Fusco et al.   PLoS Pathogens

Association of malectin with ribophorin I is crucial for attenuation of misfolded glycoprotein secretion

(2014) Koh Takeda et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

O-Linked Glycosylation of the Mucin Domain of the Herpes Simplex Virus Type 1-specific Glycoprotein gC-1 Is Temporally Regulated in a Seed-and-spread Manner

(2014) Rickard Nordén et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural Plasticity of the Semliki Forest Virus Glycome upon Interspecies Transmission

(2014) Max Crispin et al.   JOURNAL OF PROTEOME RESEARCH

Spatial Localization of the Ebola Virus Glycoprotein Mucin-Like Domain Determined by Cryo-Electron Tomography

(2014) E. E. H. Tran et al.   JOURNAL OF VIROLOGY

Biochemical composition of haemagglutinin-based influenza virus-like particle vaccine produced by transient expression in tobacco plants

(2014) François Le Mauff et al.   PLANT BIOTECHNOLOGY JOURNAL

Proteolytic and N-Glycan Processing of Human  1-Antitrypsin Expressed in Nicotiana benthamiana

(2014) A. Castilho et al.   PLANT PHYSIOLOGY

Host Controls of HIV Neutralizing Antibodies

(2014) B. F. Haynes et al.   SCIENCE

Human antibody response to N-glycans present on plant-made influenza virus-like particle (VLP) vaccines

(2014) Brian J. Ward et al.   VACCINE

Drug levels, anti-drug antibodies, and clinical efficacy of the anti-TNFα biologics in rheumatic diseases

(2013) C. C. Mok et al.   CLINICAL RHEUMATOLOGY

Anti-drug antibodies in psoriasis: a critical evaluation of clinical significance and impact on treatment response

(2013) Leeyen Hsu et al.   Expert Review of Clinical Immunology

The availability of glucose to CHO cells affects the intracellular lipid-linked oligosaccharide distribution, site occupancy and the N-glycosylation profile of a monoclonal antibody

(2013) Bo Liu et al.   JOURNAL OF BIOTECHNOLOGY

Glycosylation of closely spaced acceptor sites in human glycoproteins

(2013) S. Shrimal et al.   JOURNAL OF CELL SCIENCE

Structural basis for diverse N-glycan recognition by HIV-1–neutralizing V1–V2–directed antibody PG16

(2013) Marie Pancera et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

A method for rapid production of heteromultimeric protein complexes in plants: assembly of protective bluetongue virus-like particles

(2013) Eva C. Thuenemann et al.   PLANT BIOTECHNOLOGY JOURNAL

Rapid High-Level Production of Functional HIV Broadly Neutralizing Monoclonal Antibodies in Transient Plant Expression Systems

(2013) Yvonne Rosenberg et al.   PLoS One

Imino sugar glucosidase inhibitors as broadly active anti-filovirus agents

(2013) Jinhong Chang et al.   Emerging Microbes & Infections

Setting up a platform for plant-based influenza virus vaccine production in South Africa

(2012) Elizabeth Mortimer et al.   BMC BIOTECHNOLOGY

Engineering of Sialylated Mucin-typeO-Glycosylation in Plants

(2012) Alexandra Castilho et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Engineering Mammalian Mucin-typeO-Glycosylation in Plants

(2012) Zhang Yang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Marketing approval of mogamulizumab

(2012) Alain Beck et al.   mAbs

Moss-based production of asialo-erythropoietin devoid of Lewis A and other plant-typical carbohydrate determinants

(2012) Juliana Parsons et al.   PLANT BIOTECHNOLOGY JOURNAL

Reduction of Plant-Specific Arabinogalactan-TypeO-Glycosylation by Treating Tobacco Plants with Ferrous Chelator 2,2′-Dipyridyl

(2011) Ryo MORIGUCHI et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

N-Glycosylation engineering of plants for the biosynthesis of glycoproteins with bisected and branched complex N-glycans

(2011) Alexandra Castilho et al.   GLYCOBIOLOGY

β-N-Acetylhexosaminidases HEXO1 and HEXO3 Are Responsible for the Formation of PaucimannosidicN-Glycans inArabidopsis thaliana

(2011) Eva Liebminger et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Oligosaccharyltransferase: the central enzyme of N-linked protein glycosylation

(2011) Elisabeth Mohorko et al.   JOURNAL OF INHERITED METABOLIC DISEASE

Role of malectin in Glc2Man9GlcNAc2-dependent quality control of α1-antitrypsin

(2011) Yang Chen et al.   MOLECULAR BIOLOGY OF THE CELL

Recombinant plant-expressed tumour-associated MUC1 peptide is immunogenic and capable of breaking tolerance in MUC1.Tg mice

(2011) Julia Pinkhasov et al.   PLANT BIOTECHNOLOGY JOURNAL

Production of Complex Multiantennary N-Glycans in Nicotiana benthamiana Plants

(2011) B. Nagels et al.   PLANT PHYSIOLOGY

Site-specific characterization of threonine, serine, and tyrosine glycosylations of amyloid precursor protein/amyloid  -peptides in human cerebrospinal fluid

(2011) A. Halim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Highly conserved HIV-1 gp120 glycans proximal to CD4-binding region affect viral infectivity and neutralizing antibody induction

(2011) Xin Huang et al.   VIROLOGY

Engineering of N. benthamiana L. plants for production of N-acetylgalactosamine-glycosylated proteins - towards development of a plant-based platform for production of protein therapeutics with mucin type O-glycosylation

(2010) Sasha M Daskalova et al.   BMC BIOTECHNOLOGY

Plant glycans: friend or foe in vaccine development?

(2010) Dirk Bosch et al.   Expert Review of Vaccines

In PlantaProtein Sialylation through Overexpression of the Respective Mammalian Pathway

(2010) Alexandra Castilho et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural Basis of Cyclophilin B Binding by the Calnexin/Calreticulin P-domain

(2010) Guennadi Kozlov et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Plant-made vaccines for humans and animals

(2010) Edward P. Rybicki   PLANT BIOTECHNOLOGY JOURNAL

The production of hemagglutinin-based virus-like particles in plants: a rapid, efficient and safe response to pandemic influenza

(2010) Marc-André D’Aoust et al.   PLANT BIOTECHNOLOGY JOURNAL

Preclinical and Clinical Development of Plant-Made Virus-Like Particle Vaccine against Avian H5N1 Influenza

(2010) Nathalie Landry et al.   PLoS One

Cotranslational and Posttranslational N-Glycosylation of Polypeptides by Distinct Mammalian OST Isoforms

(2009) Catalina Ruiz-Canada et al.   CELL

Plants as biofactories for the production of subunit vaccines against bio-security-related bacteria and viruses☆

(2009) M. Manuela Rigano et al.   VACCINE

Crystal Structure and Carbohydrate Analysis of Nipah Virus Attachment Glycoprotein: a Template for Antiviral and Vaccine Design

(2008) T. A. Bowden et al.   JOURNAL OF VIROLOGY

Malectin: A Novel Carbohydrate-binding Protein of the Endoplasmic Reticulum and a Candidate Player in the Early Steps of Protein N-Glycosylation

(2008) Thomas Schallus et al.   MOLECULAR BIOLOGY OF THE CELL

Cetuximab-Induced Anaphylaxis and IgE Specific for Galactose-α-1,3-Galactose

(2008)   NEW ENGLAND JOURNAL OF MEDICINE

Influenza virus-like particles produced by transient expression inNicotiana benthamianainduce a protective immune response against a lethal viral challenge in mice

(2008) Marc-André D’Aoust et al.   PLANT BIOTECHNOLOGY JOURNAL

Generation of glyco-engineered Nicotiana benthamiana for the production of monoclonal antibodies with a homogeneous human-like N-glycan structure

(2008) Richard Strasser et al.   PLANT BIOTECHNOLOGY JOURNAL