Article
Plant Sciences
Naphatsamon Uthailak, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama
Summary: This research successfully produced functional recombinant GCase with a mannosidic-type N-glycan structure in Nicotiana benthamiana plants with downregulated N-acetylglucosaminyltransferase I gene. The glycoengineered plant line offers a promising alternative for producing therapeutic proteins targeted at the mannose receptor.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Engineering, Biomedical
Cosmin Butnarasu, Paola Petrini, Francesco Bracotti, Livia Visai, Giuseppe Guagliano, Alessandra Fiorio Pla, Ettore Sansone, Sara Petrillo, Sonja Visentin
Summary: Mucosomes, a novel class of glycosylated nanoparticles based on mucin, are introduced to overcome the complex barrier of mucus during transmucosal drug delivery. They improve drug absorption and residence time on mucosal tissues and can be loaded with different physicochemical properties. Mucosomes exhibit good mucoadhesive properties and have shown no adverse effects in in vitro and in vivo tests.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Ameer B. Taresh, Craig A. Hutton
Summary: This study presents a site-specific method for preparing N-glycosylated peptides through the incorporation of a peptide backbone thioamide linkage adjacent to an Asp residue, which facilitates site-specific conversion to N-glycosylated Asn residues in peptides promoted by Ag-I.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Jessica R. Kramer, Anna C. Deleray
Summary: This article introduces the importance of glycosylated threonine and its synthesis methods. Through high purity threonine polymers, the contributions of individual amino acids to protein structure can be explored. Structural studies have found that glycosylated threonine can induce the formation of specific conformations, shedding light on the function of rigid proteins such as mucins and collagen.
Article
Plant Sciences
Gernot Beihammer, Julia Koenig-Beihammer, Benjamin Kogelmann, Valentina Ruocco, Clemens Gruenwald-Gruber, Marc-Andre D'Aoust, Pierre-Olivier Lavoie, Pooja Saxena, Johannes S. S. Gach, Herta Steinkellner, Richard Strasser
Summary: By testing a single-subunit oligosaccharyltransferase from Leishmania donovani (LdOST), we found that it can significantly increase the Fc glycosylation level of IgG1 without changing the overall N-glycan composition. This finding is important for reducing the heterogeneity of plant-produced antibodies and improving their stability and effector functions.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Environmental Sciences
Nikoletta Mitsopoulou, Kosmas Lakiotis, Evangelia E. Golia, Ebrahim M. Khah, Ourania Pavli
Summary: The study investigated the response of Nicotiana benthamiana plants expressing SP/hrpZ(Psph) under cadmium stress. The transgenic plants demonstrated significant differences in germination rates and growth parameters compared to wild-type plants, with some lines showing superior resistance to cadmium stress. Additionally, a tissue-specific cadmium accumulation pattern was observed between wild-type and hrpZ(Psph)-plants, suggesting potential mechanisms underlying the resistance to cadmium stress.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Yanan Jiang, Zhaoxi Cheng, Si Chen, Linji Li, Weidong Zhang, Xiang Li, Honggan Hu
Summary: Among posttranslational modifications, atypical arginine N-glycosylation plays a fundamental role in cellular processes and signaling pathways. This work presents a silver-promoted solid-phase glycosylation (SGG) method for efficient synthesis of diverse arginine N-glycosylated peptides. The SSG strategy involves robust synthesis of gram-scale S-alkyl-isothiourea glycosyl donors and simultaneous introduction of side-chain sugar motif and arginine residue. The resulting toolbox facilitates the investigation of specific antibodies and provides potent biochemical tools for exploring arginine N-glycosylation.
BIOORGANIC CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Lukas Eidenberger, Florian Eminger, Alexandra Castilho, Herta Steinkellner
Summary: This study investigated the capability of three commonly used transient expression vectors to express a multi-component protein with controlled and modified N-glycosylation. The results showed that all three expression vectors produced similar quantities of targeted N-glycosylation, demonstrating the universal application of plant-based glycoengineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Takatsugu Miyazaki, Marina Ikegaya, Santiago Alonso-Gil
Summary: Glycoside hydrolase family 31 (GH31) is a diverse family of anomer-retaining alpha-glycoside hydrolases. Recently, GH31 alpha-N-acetylgalactosaminidases (Nag31s) have been found to hydrolyze mucin-type O-glycans and the crystal structure of Enterococcus faecalis Nag31 has been reported. This study investigates the substrate specificity and hydrolysis mechanism of E. faecalis Nag31, revealing its ability to release N-acetylgalactosamine from O-glycoproteins and providing insights into the diversification of sugar recognition and hydrolysis mechanisms in GH31 enzymes.
Article
Plant Sciences
Nicolo Alvisi, Kim van Noort, Sarlita Dwiani, Nathan Geschiere, Octavina Sukarta, Koen Varossieau, Dieu-Linh Nguyen, Richard Strasser, Cornelis H. Hokke, Arjen Schots, Ruud H. P. Wilbers
Summary: Recent research has shown that glycoproteins from parasitic worms can be produced transiently in Nicotiana benthamiana plants by co-expressing desired glycosyltransferases. However, efficient modification of N-glycans in plants seems to be hindered by specific enzymes.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Analytical
James W. Dressman, Colin T. McDowell, Xiaowei Lu, Peggi M. Angel, Richard R. Drake, Anand S. Mehta
Summary: N-linked glycosylation plays a crucial role in immune response and cell-to-cell interactions. The current analytical techniques for studying immune cell N-glycosylation have limitations in throughput, structural information, starting materials, and cell purification. This study proposes a rapid antibody array-based approach coupled with MALDI-IMS for analyzing cellular N-glycosylation, which provides a valuable tool for glycoimmunology research and clinical applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Plant Sciences
Kim Dua Nguyen, Hiroyuki Kajiura, Ryo Kamiya, Takahiro Yoshida, Ryo Misaki, Kazuhito Fujiyama
Summary: N-glycan engineering allows for the optimization and quality control of recombinant antibodies. Nicotiana benthamiana has emerged as an attractive production system for therapeutic antibodies. In this study, a CD27-targeting monoclonal antibody Varlilumab was successfully produced in N. benthamiana with biantennary β1,4-galactosylated N-glycan structures through N-glycan engineering.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Endocrinology & Metabolism
Leif Wide, Tord Naessen, Inger Sundstrom-Poromaa, Karin Eriksson
Summary: Women with PCOS have higher levels of LH with elevated bioactivity, leading to abnormal early follicular development, while FSH levels are decreased, contributing to arrested follicle growth.
JOURNAL OF THE ENDOCRINE SOCIETY
(2021)
Review
Plant Sciences
Richard Strasser, Georg Seifert, Monika S. Doblin, Kim L. Johnson, Colin Ruprecht, Fabian Pfrengle, Antony Bacic, Jose M. Estevez
Summary: Glycosylation is a crucial modification process that can alter the biological function of proteins and lipids, affecting the development and physiology of an organism. While challenges remain in understanding plant glycosylation, high throughput approaches may help to address these gaps, revolutionizing the identification and characterization of unknown plant glycosyltransferases and hydrolases.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Shiva Izadi, Vinny Kunnummel, Herta Steinkellner, Stefan Werner, Alexandra Castilho
Summary: There is a demand to increase manufacturing capacities for recombinant protein-based drugs. Novel expression systems such as plants are being explored for faster and more flexible production. In this study, we discuss the technical issues and solutions for achieving efficient protein sialylation in plants. We also evaluate different strategies for delivering the sialylation pathway genes and demonstrate the scalability of protein sialylation for large-scale production.
JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Immunology
Yung-Yi C. Mosley, Josiah E. Radder, Annerose Berndt, Harm HogenEsch
JOURNAL OF INFECTIOUS DISEASES
(2017)
Article
Biochemical Research Methods
Charles A. Downs, Nicholle M. Johnson, George Tsaprailis, My N. Helms
JOURNAL OF PROTEOMICS
(2018)
Editorial Material
Biochemistry & Molecular Biology
Josiah E. Radder, Steven D. Shapiro
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2019)
Article
Critical Care Medicine
Nathaniel Weathington, Michael E. O'Brien, Josiah Radder, Thomas C. Whisenant, Eugene R. Bleecker, William W. Busse, Serpil C. Erzurum, Benjamin Gaston, Annette T. Hastie, Nizar N. Jarjour, Deborah A. Meyers, Jadranka Milosevic, Wendy C. Moore, John R. Tedrow, John B. Trudeau, Hesper P. Wong, Wei Wu, Naftali Kaminski, Sally E. Wenzel, Brian D. Modena
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2019)
Article
Biochemical Research Methods
Eva Csosz, Ferenc Toth, Mohamed Mahdi, George Tsaprailis, Miklos Emri, Jozsef Tozser
BMC BIOINFORMATICS
(2019)
Article
Biochemistry & Molecular Biology
Iryna Lytvynenko, Helge Paternoga, Anna Thrun, Annika Balke, Tina A. Mueller, Christina H. Chiang, Katja Nagler, George Tsaprailis, Simon Anders, Ilka Bischofs, Julie A. Maupin-Furlow, Christian M. T. Spahn, Claudio A. P. Joazeiro
Article
Multidisciplinary Sciences
Mehdi Eshraghi, Uri Nimrod Ramirez-Jarquin, Neelam Shahani, Tommaso Nuzzo, Arianna De Rosa, Supriya Swarnkar, Nicole Galli, Oscar Rivera, George Tsaprailis, Catherina Scharager-Tapia, Gogce Crynen, Qin Li, Marie-Laure Thiolat, Erwan Bezard, Alessandro Usiello, Srinivasa Subramaniam
Article
Biochemistry & Molecular Biology
Sebastian Filbeck, Federico Cerullo, Helge Paternoga, George Tsaprailis, Claudio A. P. Joazeiro, Stefan Pfeffer
Summary: The study found that aborted translation leads to large ribosomal subunits obstructed with tRNA-linked nascent chains, which are substrates of ribosome-associated quality control. Bacterial RqcH, a widely conserved RQC factor, can modify the C-termini of nascent chains to form a polyalanine degron. The research revealed the mechanism of tRNA selection and the role of RqcH in the synthesis of nascent chains.
Article
Neurosciences
Jenna A. Levy, Christy W. LaFlamme, George Tsaprailis, Gogce Crynen, Damon T. Page
Summary: The study reveals that deletion of Dyrk1a gene in the cortex results in decreased brain mass, reduced neuronal size, structural hypoconnectivity, and autism-relevant behaviors in mice. Phosphoproteomic screening identified dysregulated growth-associated signaling cascades upon Dyrk1a deletion, and genetic suppression of Pten or pharmacological treatment with IGF-1 rescued microcephaly and neuronal undergrowth in mutant mice. These findings highlight a previously unknown mechanism through which Dyrk1a mutations disrupt growth factor signaling in the developing brain, influencing neuronal growth and connectivity.
BIOLOGICAL PSYCHIATRY
(2021)
Article
Multidisciplinary Sciences
Jingru Fang, Colette Pietzsch, Haydar Witwit, George Tsaprailis, Gogce Crynen, Kelvin Frank Cho, Alice Y. Ting, Alexander Bukreyev, Erica Ollmann Saphire, Juan Carlos de la Torre
Summary: In this study, proximity proteomics was used to define the interactome of Lassa virus polymerase, leading to the identification of proteins involved in the virus replication and transcription. Eukaryotic peptide chain release factor subunit 3a was characterized as a proviral factor that physically associates with Lassa virus polymerase. Targeted degradation of this factor resulted in strong inhibition of Lassa virus infection, suggesting it as a potential target for antiviral development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Xiaofan Zhang, John Martin Sabandal, George Tsaprailis, Ronald L. Davis
Summary: Forgetting is an important component of the brain's memory management system. This study identifies a memory suppressor gene, sickie, that functions in a dopamine neuron and supports the active forgetting process. The molecular and circuit mechanisms of forgetting are revealed, highlighting the role of sickie in maintaining presynaptic active zone structure for neurotransmitter release.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Neurosciences
Abhishek Sadhu, Kerriann K. Badal, Yibo Zhao, Adia A. Ali, Supriya Swarnkar, George Tsaprailis, Gogce C. Crynen, Sathyanarayanan Puthanveettil
Summary: Long-term memory formation requires the transport of certain proteins from the soma of a neuron to its synapses, and this study investigates the specific proteins involved and how they change in response to learning. The researchers used mass spectrometry to analyze the protein cargos of a molecular motor protein called ApKHC1 in Aplysia californica after short-term and long-term sensitization. They found that different proteins were enriched in the transport complexes at different time points, indicating experience-dependent changes in the communication between the cell body and synapses during learning.
Article
Medicine, Research & Experimental
Nathaniel M. Weathington, Diana Alvarez, John Sembrat, Josiah Radder, Nayra Cardenes, Kentaro Noda, Qiaoke Gong, Hesper Wong, Jay Kolls, Jonathan D'Cunha, Rama K. Mallampalli, Bill B. Chen, Mauricio Rojas
Article
Biochemistry & Molecular Biology
Josiah E. Radder, Alyssa D. Gregory, Adriana S. Leme, Michael H. Cho, Yanxia Chu, Neil J. Kelly, Per Bakke, Amund Gulsvik, Augusto A. Litonjua, David Sparrow, Terri H. Beaty, James D. Crapo, Edwin K. Silverman, Yingze Zhang, Annerose Berndt, Steven D. Shapiro
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2017)
Article
Critical Care Medicine
Nadine Dandachi, Neil J. Kelly, John P. Wood, Christine L. Burton, Josiah E. Radder, Adriana S. Leme, Alyssa D. Gregory, Steven D. Shapiro
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2017)