Antibody glycosylation: impact on antibody drug characteristics and quality control

Fc Sialylation Prolongs Serum Half-Life of Therapeutic Antibodies

(2019) Mathilde Bas et al.   JOURNAL OF IMMUNOLOGY

Synthetic, Zwitterionic Sp1 Oligosaccharides Adopt a Helical Structure Crucial for Antibody Interaction

(2019) Qingju Zhang et al.   ACS Central Science

Assessing the Potential Risk of Cross-Reactivity Between Anti-Bococizumab Antibodies and Other Anti-PCSK9 Monoclonal Antibodies

(2019) Ellen Q. Wang et al.   BIODRUGS

Impact of Fc N-glycan sialylation on IgG structure

(2019) Zhongqi Zhang et al.   mAbs

Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry

(2018) Yanyan Qu et al.   ANALYTICAL CHEMISTRY

Rapid development of stable transgene CHO cell lines by CRISPR/Cas9-mediated site-specific integration into C12orf35

(2018) Menglin Zhao et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Therapeutic monoclonal antibody N-glycosylation – Structure, function and therapeutic potential

(2018) Florian Cymer et al.   BIOLOGICALS

Antibody glycoengineering strategies in mammalian cells

(2018) Qiong Wang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Development and evaluation of recombinant antigen and monoclonal antibody based competition ELISA for the sero- surveillance of surra in animals

(2018) P.P. Sengupta et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Global site-specific analysis of glycoprotein N-glycan processing

(2018) Liwei Cao et al.   Nature Protocols

Polyclonal human antibodies against glycans bearing red meat-derived non-human sialic acid N-glycolylneuraminic acid are stable, reproducible, complex and vary between individuals: Total antibody levels are associated with colorectal cancer risk

(2018) Annie N. Samraj et al.   PLoS One

Glycoengineering of antibody (Herceptin) through yeast expression and in vitro enzymatic glycosylation

(2018) Chiu-Ping Liu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Sensitive and fast characterization of site-specific protein glycosylation with capillary electrophoresis coupled to mass spectrometry

(2018) Yanyan Qu et al.   TALANTA

Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins

(2018) Sharad Purohit et al.   Nature Communications

Strategies for Generating Diverse Antibody Repertoires Using Transgenic Animals Expressing Human Antibodies

(2018) Weihsu C. Chen et al.   Frontiers in Immunology

Corrigendum to Monitoring native HLA-I trimer specific antibodies in Luminex multiplex single antigen bead assay: Evaluation of beadsets from different manufacturers Journal of Immunological Methods, Volume 450, Year (2017), start page 73, end page 80

(2018) Mepur H Ravindranath et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Brief Report on the 10th Meeting of the European Forum on Antiphospholipid Antibodies

(2018) Valérie Eschwège et al.   Current Rheumatology Reports

The Mechanistic Impact of N-Glycosylation on Stability, Pharmacokinetics, and Immunogenicity of Therapeutic Proteins

(2018) Qun Zhou et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Improving Immunotherapy Through Glycodesign

(2018) Matthew J. Buettner et al.   Frontiers in Immunology

Modulation of Immune Tolerance via Siglec-Sialic Acid Interactions

(2018) Joyce Lübbers et al.   Frontiers in Immunology

Producing defucosylated antibodies with enhanced in vitro antibody-dependent cellular cytotoxicity via FUT8 knockout CHO-S cells

(2017) Huifang Zong et al.   ENGINEERING IN LIFE SCIENCES

Identification of Fc Gamma Receptor Glycoforms That Produce Differential Binding Kinetics for Rituximab

(2017) Jerrard M. Hayes et al.   MOLECULAR & CELLULAR PROTEOMICS

The Immunoregulatory Roles of Antibody Glycosylation

(2017) Madeleine F. Jennewein et al.   TRENDS IN IMMUNOLOGY

Production of Functional Anti-Ebola Antibodies in Pichia pastoris

(2017) Oliver Purcell et al.   ACS Synthetic Biology

Integrated Genome and Protein Editing Swaps α -2,6 Sialylation for α -2,3 Sialic Acid on Recombinant Antibodies from CHO

(2017) Cheng-yu Chung et al.   Biotechnology Journal

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

(2017) Javier Barallobre-Barreiro et al.   Jove-Journal of Visualized Experiments

Decoding the Human Immunoglobulin G-Glycan Repertoire Reveals a Spectrum of Fc-Receptor- and Complement-Mediated-Effector Activities

(2017) Gillian Dekkers et al.   Frontiers in Immunology

Fc-Galactosylation of Human Immunoglobulin Gamma Isotypes Improves C1q Binding and Enhances Complement-Dependent Cytotoxicity

(2017) Benjamin Peschke et al.   Frontiers in Immunology

Anti-Neu5Gc and anti-non-Neu5Gc antibodies in healthy humans

(2017) Bingsi Gao et al.   PLoS One

Glycoengineering of pertuzumab and its impact on the pharmacokinetic/pharmacodynamic properties

(2017) Cheng Luo et al.   Scientific Reports

N-glycosylation heterogeneity and the influence on structure, function and pharmacokinetics of monoclonal antibodies and Fc fusion proteins

(2016) Fabian Higel et al.   EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

Liquid chromatography-tandem mass spectrometry-based fragmentation analysis of glycopeptides

(2016) Jonas Nilsson   GLYCOCONJUGATE JOURNAL

Applying mini-bore HPAEC-MS/MS for the characterization and quantification of Fc N -glycans from heterogeneously glycosylated IgGs

(2016) Maria Maier et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Enhanced sialylation of a human chimeric IgG1 variant produced in human and rodent cell lines

(2016) Yusuke Mimura et al.   JOURNAL OF IMMUNOLOGICAL METHODS

The Emerging Importance of IgG Fab Glycosylation in Immunity

(2016) Fleur S. van de Bovenkamp et al.   JOURNAL OF IMMUNOLOGY

Ultrafast and high-throughput N-glycan analysis for monoclonal antibodies

(2016) Xiaoyu Yang et al.   mAbs

Increased in vivo effector function of human IgG4 isotype antibodies through afucosylation

(2016) Qian Gong et al.   mAbs

The use of lectin microarray for assessing glycosylation of therapeutic proteins

(2016) Lei Zhang et al.   mAbs

Human risk of diseases associated with red meat intake: Analysis of current theories and proposed role for metabolic incorporation of a non-human sialic acid

(2016) Frederico Alisson-Silva et al.   MOLECULAR ASPECTS OF MEDICINE

Fc-galactosylation modulates antibody-dependent cellular cytotoxicity of therapeutic antibodies

(2016) Marco Thomann et al.   MOLECULAR IMMUNOLOGY

The Mannose Receptor Is Involved in the Phagocytosis of Mycobacteria-Induced Apoptotic Cells

(2016) Teresa Garcia-Aguilar et al.   Journal of Immunology Research

Comparison of procainamide and 2-aminobenzamide labeling for profiling and identification of glycans by liquid chromatography with fluorescence detection coupled to electrospray ionization–mass spectrometry

(2015) Radoslaw P. Kozak et al.   ANALYTICAL BIOCHEMISTRY

Rapid Preparation of Released N-Glycans for HILIC Analysis Using a Labeling Reagent that Facilitates Sensitive Fluorescence and ESI-MS Detection

(2015) Matthew A. Lauber et al.   ANALYTICAL CHEMISTRY

Functional knockout of FUT8 in Chinese hamster ovary cells using CRISPR/Cas9 to produce a defucosylated antibody

(2015) Tao Sun et al.   ENGINEERING IN LIFE SCIENCES

Fc glycans of therapeutic antibodies as critical quality attributes

(2015) Dietmar Reusch et al.   GLYCOBIOLOGY

The alpha-gal story: Lessons learned from connecting the dots

(2015) John W. Steinke et al.   JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

Glycan analysis of therapeutic glycoproteins

(2015) Lei Zhang et al.   mAbs

Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3)

(2015) Rosina Plomp et al.   MOLECULAR & CELLULAR PROTEOMICS

Engineered CHO cells for production of diverse, homogeneous glycoproteins

(2015) Zhang Yang et al.   NATURE BIOTECHNOLOGY

Mass spectrometry for glycosylation analysis of biopharmaceuticals

(2015) Viktoria Dotz et al.   TRAC-TRENDS IN ANALYTICAL CHEMISTRY

Allergic/infusion reactions reported with cetuximab and rituximab

(2015) Ray A Wolf et al.   World Allergy Organization Journal

Glycoengineered Monoclonal Antibodies with Homogeneous Glycan (M3, G0, G2, and A2) Using a Chemoenzymatic Approach Have Different Affinities for FcγRIIIa and Variable Antibody-Dependent Cellular Cytotoxicity Activities

(2015) Masaki Kurogochi et al.   PLoS One

Multi-Angle Effector Function Analysis of Human Monoclonal IgG Glycovariants

(2015) Tetyana Dashivets et al.   PLoS One

Impact of Glycation on Antibody Clearance

(2014) Jane Yang et al.   AAPS Journal

Sample preparation for mass spectrometric analysis of human serum N-glycans using hydrophilic interaction chromatography-based solid phase extraction

(2014) Liwei Cao et al.   ANALYST

Effect of glucose feeding on the glycosylation quality of antibody produced by a human cell line, F2N78, in fed-batch culture

(2014) Jin Seok Seo et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Comparison of separation techniques for the elucidation of IgG N-glycans pooled from healthy mammalian species

(2014) Barbara Adamczyk et al.   CARBOHYDRATE RESEARCH

Effects of nutrient levels and average culture pH on the glycosylation pattern of camelid-humanized monoclonal antibody

(2014) Hengameh Aghamohseni et al.   JOURNAL OF BIOTECHNOLOGY

Glycan Analysis of Prostate Specific Antigen (PSA) Directly from the Intact Glycoprotein by HR-ESI/TOF-MS

(2014) Henning N. Behnken et al.   JOURNAL OF PROTEOME RESEARCH

Comparison of methods for the analysis of therapeutic immunoglobulin G Fc-glycosylation profiles—Part 1: Separation-based methods

(2014) Dietmar Reusch et al.   mAbs

Effect of culture pH on recombinant antibody production by a new human cell line, F2N78, grown in suspension at 33.0 °C and 37.0 °C

(2013) Jin Seok Seo et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Adaptive immune activation: glycosylation does matter

(2013) Margreet A Wolfert et al.   Nature Chemical Biology

Ligand Binding and Signaling of Dendritic Cell Immunoreceptor (DCIR) Is Modulated by the Glycosylation of the Carbohydrate Recognition Domain

(2013) Karien Bloem et al.   PLoS One

A Dual-Mode Surface Display System for the Maturation and Production of Monoclonal Antibodies in Glyco-Engineered Pichia pastoris

(2013) Hussam H. Shaheen et al.   PLoS One

Revisiting the Role of Glycosylation in the Structure of Human IgG Fc

(2012) M. Jack Borrok et al.   ACS Chemical Biology

Recent structural and mechanistic insights into post-translational enzymatic glycosylation

(2012) Ramon Hurtado-Guerrero et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Mogamulizumab

(2012) Joshuan M. Subramaniam et al.   DRUGS

Galactosylation variations in marketed therapeutic antibodies

(2012) T. Shantha Raju et al.   mAbs

Production, characterization and pharmacokinetic properties of antibodies with N-linked Mannose-5 glycans

(2012) Marcella Yu et al.   mAbs

Generation and Comparative Characterization of Glycosylated and Aglycosylated Human IgG1 Antibodies

(2012) Dmitrij Hristodorov et al.   MOLECULAR BIOTECHNOLOGY

The Impact of Glycosylation on the Pharmacokinetics of a TNFR2:Fc Fusion Protein Expressed in Glycoengineered Pichia Pastoris

(2012) Liming Liu et al.   PHARMACEUTICAL RESEARCH

Loss of Response and Need for Adalimumab Dose Intensification in Crohn's Disease: A Systematic Review

(2011) Vincent Billioud et al.   AMERICAN JOURNAL OF GASTROENTEROLOGY

Rapid High-Resolution Characterization of Functionally Important Monoclonal AntibodyN-Glycans by Capillary Electrophoresis

(2011) Zoltan Szabo et al.   ANALYTICAL CHEMISTRY

Modulation of antibody galactosylation through feeding of uridine, manganese chloride, and galactose

(2011) Michael J. Gramer et al.   BIOTECHNOLOGY AND BIOENGINEERING

High-mannose glycans on the Fc region of therapeutic IgG antibodies increase serum clearance in humans

(2011) A. M. Goetze et al.   GLYCOBIOLOGY

Chemoenzymatic Synthesis and Fcγ Receptor Binding of Homogeneous Glycoforms of Antibody Fc Domain. Presence of a Bisecting Sugar Moiety Enhances the Affinity of Fc to FcγIIIa Receptor

(2011) Guozhang Zou et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biochemical and biophysical characterization of humanized IgG1 produced inPichia pastoris

(2011) Sha Ha et al.   mAbs

Glycoengineered Pichia produced anti-HER2 is comparable to trastuzumab in preclinical study

(2011) Ningyan Zhang et al.   mAbs

HILIC analysis of fluorescence-labeled N-glycans from recombinant biopharmaceuticals

(2010) Michael Melmer et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Glycan labeling strategies and their use in identification and quantification

(2010) L. R. Ruhaak et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

The evaluation of a novel approach for the profiling and identification of N-linked glycan with a procainamide tag by HPLC with fluorescent and mass spectrometric detection

(2010) Song Klapoetke et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Loss of Response and Requirement of Infliximab Dose Intensification in Crohn's Disease: A Review

(2009) Javier P Gisbert et al.   AMERICAN JOURNAL OF GASTROENTEROLOGY

Recombinant antibody therapeutics: the impact of glycosylation on mechanisms of action

(2009) Royston Jefferis   TRENDS IN PHARMACOLOGICAL SCIENCES

Anti-Inflammatory Actions of Intravenous Immunoglobulin

(2008) Falk Nimmerjahn et al.   Annual Review of Immunology

Diversity in specificity, abundance, and composition of anti-Neu5Gc antibodies in normal humans: Potential implications for disease

(2008) V. Padler-Karavani et al.   GLYCOBIOLOGY

Cetuximab-Induced Anaphylaxis and IgE Specific for Galactose-α-1,3-Galactose

(2008) Christine H. Chung et al.   NEW ENGLAND JOURNAL OF MEDICINE

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

(2008) Gaurav Backliwal et al.   NUCLEIC ACIDS RESEARCH

Generation of glyco-engineered Nicotiana benthamiana for the production of monoclonal antibodies with a homogeneous human-like N-glycan structure

(2008) Richard Strasser et al.   PLANT BIOTECHNOLOGY JOURNAL

Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides

(2008) Johannes Stadlmann et al.   PROTEOMICS

The Galα1,3Galβ1,4GlcNAc-R (α-Gal) epitope: A carbohydrate of unique evolution and clinical relevance

(2007) Bruce A. Macher et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS