Rapid development of stable transgene CHO cell lines by CRISPR/Cas9-mediated site-specific integration into C12orf35

Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems

(2018) Yoshinori Kawabe et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Producing defucosylated antibodies with enhanced in vitro antibody-dependent cellular cytotoxicity via FUT8 knockout CHO-S cells

(2017) Huifang Zong et al.   ENGINEERING IN LIFE SCIENCES

Accumulative scFv-Fc antibody gene integration into the hprt chromosomal locus of Chinese hamster ovary cells

(2017) Xue Wang et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Rapid characterization of the CHO platform cell line and identification of pseudo attP sites for PhiC31 integrase

(2017) Narges Damavandi et al.   PROTEIN EXPRESSION AND PURIFICATION

What can mathematical modelling say about CHO metabolism and protein glycosylation?

(2017) Sarah N. Galleguillos et al.   Computational and Structural Biotechnology Journal

Production process reproducibility and product quality consistency of transient gene expression in HEK293 cells with anti-PD1 antibody as the model protein

(2016) Kai Ding et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

mTORC1 signalling and eIF4E/4E-BP1 translation initiation factor stoichiometry influence recombinant protein productivity from GS-CHOK1 cells

(2016) L. Josse et al.   BIOCHEMICAL JOURNAL

Targeted transgene insertion into the CHO cell genome using Cre recombinase-incorporating integrase-defective retroviral vectors

(2016) Yoshinori Kawabe et al.   BIOTECHNOLOGY AND BIOENGINEERING

Accelerated homology-directed targeted integration of transgenes in Chinese hamster ovary cells via CRISPR/Cas9 and fluorescent enrichment

(2016) Jae Seong Lee et al.   BIOTECHNOLOGY AND BIOENGINEERING

Disruption of the gene C12orf35 leads to increased productivities in recombinant CHO cell lines

(2016) Anett Ritter et al.   BIOTECHNOLOGY AND BIOENGINEERING

Identifying and retargeting transcriptional hot spots in the human genome

(2016) Joseph K. Cheng et al.   Biotechnology Journal

Gene editing for cell engineering: trends and applications

(2016) Sanjeev K. Gupta et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes

(2016) Harumi Nakao et al.   GENESIS

High-density mammalian cell cultures in stirred-tank bioreactor without external pH control

(2016) Sen Xu et al.   JOURNAL OF BIOTECHNOLOGY

A method for specifically targeting two independent genomic integration sites for co-expression of genes in CHO cells

(2016) Bahar Baser et al.   METHODS

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair

(2016) Xiangjun He et al.   NUCLEIC ACIDS RESEARCH

FISH-Based Analysis of Clonally Derived CHO Cell Populations Reveals High Probability for Transgene Integration in a Terminal Region of Chromosome 1 (1q13)

(2016) Shengwei Li et al.   PLoS One

Identifying and retargeting transcriptional hot spots in the human genome

(2016) Joseph K. Cheng et al.   Biotechnology Journal

The art of CHO cell engineering: A comprehensive retrospect and future perspectives

(2015) Simon Fischer et al.   BIOTECHNOLOGY ADVANCES

CRISPR-Cas targeted plasmid integration into mammalian cells via non-homologous end joining

(2015) Ravichandra Bachu et al.   BIOTECHNOLOGY AND BIOENGINEERING

Functional knockout of FUT8 in Chinese hamster ovary cells using CRISPR/Cas9 to produce a defucosylated antibody

(2015) Tao Sun et al.   ENGINEERING IN LIFE SCIENCES

Zinc finger nuclease technology: Advances and obstacles in modelling and treating genetic disorders

(2015) Hamid Reza Jabalameli et al.   GENE

Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids

(2015) Tetsushi Sakuma et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo

(2015) Miguel A Moreno-Mateos et al.   NATURE METHODS

Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway

(2015) Jae Seong Lee et al.   Scientific Reports

Precise and efficient genome editing in zebrafish using the CRISPR/Cas9 system

(2014) U. Irion et al.   DEVELOPMENT

The impact of anti-apoptotic gene Bcl-2∆ expression on CHO central metabolism

(2014) Neil Templeton et al.   METABOLIC ENGINEERING

In Vitro Characterization of the Anti-PD-1 Antibody Nivolumab, BMS-936558, and In Vivo Toxicology in Non-Human Primates

(2014) C. Wang et al.   Cancer Immunology Research

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

(2013) Prashant Mali et al.   NATURE BIOTECHNOLOGY

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Mammalian cell protein expression for biopharmaceutical production

(2011) Jianwei Zhu   BIOTECHNOLOGY ADVANCES