Producing defucosylated antibodies with enhanced in vitro antibody-dependent cellular cytotoxicity via FUT8 knockout CHO-S cells

Production process reproducibility and product quality consistency of transient gene expression in HEK293 cells with anti-PD1 antibody as the model protein

(2016) Kai Ding et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

CRISPR-Cas targeted plasmid integration into mammalian cells via non-homologous end joining

(2015) Ravichandra Bachu et al.   BIOTECHNOLOGY AND BIOENGINEERING

CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives

(2015) Jae Seong Lee et al.   Biotechnology Journal

Phase 1/2 study of mogamulizumab, a defucosylated anti-CCR4 antibody, in previously treated patients with cutaneous T-cell lymphoma

(2015) M. Duvic et al.   BLOOD

Functional knockout of FUT8 in Chinese hamster ovary cells using CRISPR/Cas9 to produce a defucosylated antibody

(2015) Tao Sun et al.   ENGINEERING IN LIFE SCIENCES

Core Fucosylation of IgG B Cell Receptor Is Required for Antigen Recognition and Antibody Production

(2015) Wenzhe Li et al.   JOURNAL OF IMMUNOLOGY

Morphological and molecular characteristics of HER2 amplified urothelial bladder cancer

(2015) J. Tschui et al.   VIRCHOWS ARCHIV

CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives

(2015) Jae Seong Lee et al.   Biotechnology Journal

Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool

(2014) Carlotta Ronda et al.   BIOTECHNOLOGY AND BIOENGINEERING

Characterization of Genomic Deletion Efficiency Mediated by Clustered Regularly Interspaced Palindromic Repeats (CRISPR)/Cas9 Nuclease System in Mammalian Cells

(2014) Matthew C. Canver et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Expanding the genetic editing tool kit: ZFNs, TALENs, and CRISPR-Cas9

(2014) Rajat M. Gupta et al.   JOURNAL OF CLINICAL INVESTIGATION

Biacore surface matrix effects on the binding kinetics and affinity of an antigen/antibody complex

(2012) Andrew W. Drake et al.   ANALYTICAL BIOCHEMISTRY

Donor substrate binding and enzymatic mechanism of human core α1,6-fucosyltransferase (FUT8)

(2012) Miriam P. Kötzler et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Marketing approval of mogamulizumab

(2012) Alain Beck et al.   mAbs

Trastuzumab induces antibody-dependent cell-mediated cytotoxicity (ADCC) in HER-2-non-amplified breast cancer cell lines

(2011) D. M. Collins et al.   ANNALS OF ONCOLOGY

Mammalian cell protein expression for biopharmaceutical production

(2011) Jianwei Zhu   BIOTECHNOLOGY ADVANCES

Therapeutic antibodies: Market considerations, disease targets and bioprocessing

(2011) John G. Elvin et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

Highly efficient deletion of FUT8 in CHO cell lines using zinc-finger nucleases yields cells that produce completely nonfucosylated antibodies

(2010) Laetitia Malphettes et al.   BIOTECHNOLOGY AND BIOENGINEERING

Cell Engineering and Cultivation of Chinese Hamster Ovary (CHO) Cells

(2010) Takeshi Omasa et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

The bioanalysis of the monoclonal antibody trastuzumab by high-performance liquid chromatography with fluorescence detection after immuno-affinity purification from human serum

(2009) Carola W.N. Damen et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Variable Contribution of Monoclonal Antibodies to ADCC in patients with chronic lymphocytic leukemia

(2009) James Weitzman et al.   LEUKEMIA & LYMPHOMA

Glycosylation as a strategy to improve antibody-based therapeutics

(2009) Roy Jefferis   NATURE REVIEWS DRUG DISCOVERY