Article
Microbiology
Yixiao Huang, Fanchao Zhu, Jin Koh, Daniel Stanton, Sixue Chen, Nian Wang
Summary: This study reveals that Citrus Huanglongbing (HLB) is caused by Candidatus Liberibacter asiaticus (Las) triggering immune responses rather than directly containing pathogenicity factors. The researchers used Liberibacter crescens (Lcr) as a surrogate to identify proteins of Las and discovered potential proteins that may interact with host proteins in the phloem tissues.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biology
Layla Drwesh, Benjamin Heim, Max Graf, Linda Kehr, Lea Hansen-Palmus, Mirita Franz-Wachtel, Boris Macek, Hubert Kalbacher, Johannes Buchner, Doron Rapaport
Summary: This study reconstituted the early cytosolic steps of signal-anchored (SA) protein biogenesis and identified molecular (co)chaperones, including Hsp70, Hsp90, and Hsp40 family co-chaperones, that interact with newly synthesized SA proteins. These interactions are mediated by the hydrophobic transmembrane segments of the SA proteins. The study also demonstrated the inhibitory effect of interfering with these interactions on SA protein biogenesis and successfully reconstituted the transfer of peptides from Hsp70 chaperone to the mitochondrial Tom70 receptor in vitro.
Article
Biochemistry & Molecular Biology
Olga Jovanovic, Ksenia Chekashkina, Sanja Skulj, Kristina Zuna, Mario Vazdar, Pavel V. V. Bashkirov, Elena E. E. Pohl
Summary: Oxidative stress and ROS play important roles in the pathogenesis of various diseases by directly altering proteins and affecting lipids. This study reveals that oxidative stress decreases stored curvature elastic stress (SCES) in lipid bilayers, leading to increased protonophoric activity of uncoupling proteins UCP1 and ANT1.
Article
Multidisciplinary Sciences
Uwe Schulte, Fabian den Brave, Alexander Haupt, Arushi Gupta, Jiyao Song, Catrin S. S. Mueller, Jeannine Engelke, Swadha Mishra, Christoph Martensson, Lars Ellenrieder, Chantal Priesnitz, Sebastian P. P. Straub, Kim Nguyen Doan, Bogusz Kulawiak, Wolfgang Bildl, Heike Rampelt, Nils Wiedemann, Nikolaus Pfanner, Bernd Fakler, Thomas Becker
Summary: This study presents a quantitative mapping of mitochondrial protein assemblies using high-resolution complexome profiling. The results reveal the complex organization of mitochondrial protein assemblies involved in various cellular processes. It also identifies quality-control factors and elucidates a constitutive pathway for preprotein removal.
Article
Multidisciplinary Sciences
Hazel Stewart, Yongxu Lu, Sarah O'Keefe, Anusha Valpadashi, Luis Daniel Cruz-Zaragoza, Hendrik A. Michel, Samantha K. Nguyen, George W. Carnell, Nina Lukhovitskaya, Rachel Milligan, Yasmin Adewusi, Irwin Jungreis, Valeria Lulla, David A. Matthews, Stephen High, Peter Rehling, Edward Emmott, Jonathan L. Heeney, Andrew D. Davidson, James R. Edgar, Geoffrey L. Smith, Andrew E. Firth
Summary: The SARS-CoV-2 genome encodes multiple accessory proteins, including a previously uncharacterized ORF3c protein. ORF3c localizes to mitochondria and inhibits innate immunity by restricting IFN-beta production. It has inhibitory effects after stimulation with cytoplasmic RNA helicases RIG-I or MDA5 or adaptor protein MAVS, but not under other stimulation conditions.
Article
Cell Biology
Olivia M. Connor, Srujan K. Matta, Jonathan R. Friedman
Summary: This study reveals the importance of a protein called Mdi1 in coordinating mitochondrial fission. Loss of Mdi1 leads to mitochondrial hyperfusion, suggesting its role in regulating fission. This work highlights the crucial role of Mdi1 in efficient mitochondrial division.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Neurosciences
Avanti Gokhale, Chelsea E. Lee, Stephanie A. Zlatic, Amanda A. H. Freeman, Nicole Shearing, Cortnie Hartwig, Oluwaseun Ogunbona, Julia L. Bassell, Meghan E. Wynne, Erica Werner, Chongchong Xu, Zhexing Wen, Duc Duong, Nicholas T. Seyfried, Carrie E. Bearden, Viktor Janos Olah, Matthew J. M. Rowan, Jill R. Glausier, David A. Lewis, Victor Faundez
Summary: This study identified the importance of genes SLC25A1 and MRPL40, present in the deleted segment of 22q11.2 microdeletion syndrome, in maintaining mitochondrial ribosomal integrity and proteostasis, which are crucial for synaptic neurodevelopment and function. Fruit fly experiments demonstrated that mitochondrial ribosome function is essential for synaptic neurodevelopment, function, and behavior. Mitochondrial proteostasis perturbations, caused by genetic or environmental factors, may contribute to the pathogenesis of neurodevelopmental disorders.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Dung T. Huynh, Wouter S. P. Jong, Gregory M. Koningstein, Peter van Ulsen, Joen Luirink
Summary: Ct-MOMP, a promising subunit-based vaccine candidate, is difficult to express in its native structure in E. coli outer membrane. Co-expression of the Bam complex improved the expression and localization of recombinant Ct-MOMP, leading to correct folding and assembly into a beta-barrel conformation at the cell surface.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Inna Aphasizheva, Ruslan Aphasizhev
Summary: Trypanosoma brucei, a unicellular parasite, causes a range of diseases in African human and animal populations, posing a threat to public health and impacting the economy in sub-Saharan Africa. The organism is characterized by a single mitochondrion containing a kinetoplast nucleoid, which consists of densely packed DNA molecules called kinetoplast DNA (kDNA). The kDNA network includes maxicircles encoding rRNAs and proteins, as well as minicircles bearing gRNA genes.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2021)
Article
Cell Biology
Jialin Zhou, Martin Jung, Kai S. Dimmer, Doron Rapaport
Summary: In this study, we investigated the biogenesis of multi-span proteins in the mitochondrial outer membrane. Our findings suggest that the targeting specificity and efficiency of these proteins are influenced by their length, as well as the behavior of the membrane. Additionally, cytosolic proteins do not significantly contribute to the biogenesis process.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Victor V. Lemeshko
Summary: Recently reported kinase-linked mild depolarization of mitochondria may be a crucial component of the mitochondrial anti-aging program. However, the current measurement methods for mitochondrial inner membrane potential ignore the possibility of outer membrane potential generation. The experimental data reported in this study may reflect changes in the positive outer membrane potential generated by VDAC-kinase complexes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2022)
Review
Biochemistry & Molecular Biology
Jakob D. Busch, Laura F. Fielden, Nikolaus Pfanner, Nils Wiedemann
Summary: Biogenesis of mitochondria involves the import of numerous precursor proteins across the mitochondrial membranes. Different import pathways exist, ranging from presequence-directed pathway to pathways using internal or carboxy-terminal targeting signals. Recent studies have revealed the structural organization of membrane-embedded preprotein translocases and their dynamic interactions with other cellular machineries. These insights provide valuable knowledge about the mechanisms of mitochondrial protein import.
Article
Multidisciplinary Sciences
Kazuya Nishio, Tomoyuki Kawarasaki, Yuki Sugiura, Shunsuke Matsumoto, Ayano Konoshima, Yuki Takano, Mayuko Hayashi, Fumihiko Okumura, Takumi Kamura, Tsunehiro Mizushima, Kunio Nakatsukasa
Summary: Deficiencies in mitochondrial protein import are associated with diseases, but it is unclear how accumulation of non-imported mitochondrial proteins causes cell dysfunction. This study showed that non-imported citrate synthase is targeted for proteasomal degradation. Accumulation of non-imported citrate synthase caused ectopic citrate synthesis, leading to metabolic imbalance and growth defect. This study highlights the importance of mitochondrial import and its failure in cellular metabolism.
Review
Biochemistry & Molecular Biology
Shashank Dadsena, Andreas Jenner, Ana J. Garcia-Saez
Summary: Apoptotic cell death plays a crucial role in development and tissue homeostasis, and MOMP is a central event in this process. Researchers focus on the interaction network of Bcl-2 family proteins and other regulatory elements, emphasizing the importance of single-molecule techniques in uncovering apoptosis regulation mechanisms.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Qiongqiong Bai, Han Li, Xiling Wu, Jihong Shao, Mingjun Sun, Dehui Yin
Summary: This study demonstrated that omp31 and BP26 are potential candidate antigens with high application value in the clinical diagnosis of brucellosis, showing improved accuracy in detecting the disease.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Vladimir Varga, Flavia Moreira-Leite, Neil Portman, Keith Gull
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Multidisciplinary Sciences
Beatrice Freya Lucy Edwards, Richard John Wheeler, Amy Rachel Barker, Flavia Fernandes Moreira-Leite, Keith Gull, Jack Daniel Sunter
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Infectious Diseases
Fernanda Cristina Costa, Amanda Fortes Francisco, Shiromani Jayawardhana, Simone Guedes Calderano, Michael D. Lewis, Francisco Olmo, Tom Beneke, Eva Gluenz, Jack Sunter, Samuel Dean, John Morrison Kelly, Martin Craig Taylor
PLOS NEGLECTED TROPICAL DISEASES
(2018)
Article
Cell Biology
Calvin Tiengwe, Carolina M. Koeller, James D. Bangs
MOLECULAR BIOLOGY OF THE CELL
(2018)
Article
Biochemistry & Molecular Biology
Jack Daniel Sunter, Flavia Moreira-Leite, Keith Gull
Article
Multidisciplinary Sciences
Jack D. Sunter, Ryuji Yanase, Ziyin Wang, Carolina Moura Costa Catta-Preta, Flavia Moreira-Leite, Jitka Myskova, Katerina Pruzinova, Petr Volf, Jeremy C. Mottram, Keith Gull
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biology
Samuel Dean, Flavia Moreira-Leite, Keith Gull
Article
Biochemistry & Molecular Biology
Movin Abeywickrema, Hana Vachova, Helen Farr, Timm Mohr, Richard J. Wheeler, De-Hua Lai, Sue Vaughan, Keith Gull, Jack D. Sunter, Vladimir Varga
MOLECULAR MICROBIOLOGY
(2019)
Review
Microbiology
Richard J. Wheeler, Keith Gull, Jack D. Sunter
ANNUAL REVIEW OF MICROBIOLOGY, VOL 73
(2019)
Editorial Material
Microbiology
Calvin Tiengwe
Article
Biochemistry & Molecular Biology
Carolina M. Koeller, Calvin Tiengwe, Kevin J. Schwartz, James D. Bangs
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Cell Biology
Ziyin Wang, Tom Beneke, Eva Gluenz, Richard John Wheeler
JOURNAL OF CELL SCIENCE
(2020)
Article
Microbiology
Carla Gilabert Carbajo, Lucy J. Cornell, Youssef Madbouly, Zhihao Lai, Phillip A. Yates, Michele Tinti, Calvin Tiengwe
Summary: Iron deprivation in Trypanosoma brucei induces upregulation of TfR, parasite-specific genes, glucose uptake and glycolysis genes, endocytosis genes, and a divergent RNA binding protein RBP5. Cells depleted of TfR import free iron as a survival strategy. RBP5 expression is regulated post-transcriptionally and above a certain threshold is toxic, affecting cell cycle progression. This study sheds new light on the mechanisms by which T. brucei handles iron stress.
Article
Cell Biology
Ciaran J. McCoy, Humbeline Paupelin-Vaucelle, Peter Gorilak, Tom Beneke, Vladimir Varga, Eva Gluenz
Summary: ULK kinase family proteins are involved in the function of motile cilia. Knockout mutants of ULK4 and Fused genes in Leishmania mexicana showed structural defects in the flagellum cytoskeleton. The interaction between ULK4 and Fused proteins suggests that ULK4 acts as a positive regulator of Fused kinase in the assembly of motile cilia.
MOLECULAR BIOLOGY OF THE CELL
(2023)
Correction
Biochemistry & Molecular Biology
Jack Sunter, Keith Gull