Expanding the toolbox for Trypanosoma cruzi: A parasite line incorporating a bioluminescence-fluorescence dual reporter and streamlined CRISPR/Cas9 functionality for rapid in vivo localisation and phenotyping

Rapid, Selection-Free, High-Efficiency Genome Editing in Protozoan Parasites Using CRISPR-Cas9 Ribonucleoproteins

(2017) Lia Carolina Soares Medeiros et al.   mBio

A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids

(2017) Tom Beneke et al.   Royal Society Open Science

Optimized CRISPR-Cas9 Genome Editing for Leishmania and Its Use To Target a Multigene Family, Induce Chromosomal Translocation, and Study DNA Break Repair Mechanisms

(2017) Wen-Wei Zhang et al.   mSphere

Host and parasite genetics shape a link betweenTrypanosoma cruziinfection dynamics and chronic cardiomyopathy

(2016) Michael D. Lewis et al.   CELLULAR MICROBIOLOGY

CRISPR/Cas9-mediated endogenous C-terminal Tagging ofTrypanosoma cruziGenes Reveals the Acidocalcisome Localization of the Inositol 1,4,5-Trisphosphate Receptor

(2016) Noelia Lander et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Proteasome inhibition for treatment of leishmaniasis, Chagas disease and sleeping sickness

(2016) Shilpi Khare et al.   NATURE

Killer lymphocytes use granulysin, perforin and granzymes to kill intracellular parasites

(2016) Farokh Dotiwala et al.   NATURE MEDICINE

mScarlet: a bright monomeric red fluorescent protein for cellular imaging

(2016) Daphne S Bindels et al.   NATURE METHODS

Putting Infection Dynamics at the Heart of Chagas Disease

(2016) Michael D. Lewis et al.   TRENDS IN PARASITOLOGY

Nitroheterocyclic drugs cure experimental Trypanosoma cruzi infections more effectively in the chronic stage than in the acute stage

(2016) Amanda Fortes Francisco et al.   Scientific Reports

Limited Ability of Posaconazole To Cure both Acute and Chronic Trypanosoma cruzi Infections Revealed by Highly SensitiveIn VivoImaging

(2015) Amanda Fortes Francisco et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

A method for fixing and paraffin embedding tissue to retain the natural fluorescence of reporter proteins

(2015) Akifumi Nakagawa et al.   BIOTECHNIQUES

First efficient CRISPR-Cas9-mediated genome editing inLeishmaniaparasites

(2015) Lauriane Sollelis et al.   CELLULAR MICROBIOLOGY

CRISPR-Cas9-Mediated Genome Editing in Leishmania donovani

(2015) Wen-Wei Zhang et al.   mBio

CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 Genes in Trypanosoma cruzi Reveals Their Role in Flagellar Attachment

(2015) Noelia Lander et al.   mBio

A toolkit enabling efficient, scalable and reproducible gene tagging in trypanosomatids

(2015) S. Dean et al.   Open Biology

The Trypanosoma cruzi Vitamin C Dependent Peroxidase Confers Protection against Oxidative Stress but Is Not a Determinant of Virulence

(2015) Martin C. Taylor et al.   PLoS Neglected Tropical Diseases

A Clinical Adverse Drug Reaction Prediction Model for Patients with Chagas Disease Treated with Benznidazole

(2014) Gilberto Marcelo Sperandio da Silva et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Bioluminescence imaging of chronicTrypanosoma cruziinfections reveals tissue-specific parasite dynamics and heart disease in the absence of locally persistent infection

(2014) Michael D. Lewis et al.   CELLULAR MICROBIOLOGY

CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in Trypanosoma cruzi

(2014) D. Peng et al.   mBio

A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum

(2013) Nathan C Shaner et al.   NATURE METHODS

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Highly Sensitive In Vivo Imaging of Trypanosoma brucei Expressing “Red-Shifted” Luciferase

(2013) Alex P. McLatchie et al.   PLoS Neglected Tropical Diseases

Trypanosoma cruzi and Chagas' Disease in the United States

(2011) C. Bern et al.   CLINICAL MICROBIOLOGY REVIEWS

RNA Interference in Protozoan Parasites: Achievements and Challenges

(2011) Nikolay G. Kolev et al.   EUKARYOTIC CELL

Genome-wide RNAi screens in bloodstream form trypanosomes identify drug transporters

(2010) Gabriela Schumann Burkard et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

TriTrypDB: a functional genomic resource for the Trypanosomatidae

(2009) Martin Aslett et al.   NUCLEIC ACIDS RESEARCH

Feasibility, Drug Safety, and Effectiveness of Etiological Treatment Programs for Chagas Disease in Honduras, Guatemala, and Bolivia: 10-Year Experience of Médecins Sans Frontières

(2009) Oliver Yun et al.   PLoS Neglected Tropical Diseases

New, Improved Treatments for Chagas Disease: From the R&D Pipeline to the Patients

(2009) Isabela Ribeiro et al.   PLoS Neglected Tropical Diseases

Single-locus targeting constructs for reliable regulated RNAi and transgene expression in Trypanosoma brucei

(2008) Sam Alsford et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Drug-induced cure drives conversion to a stable and protective CD8+ T central memory response in chronic Chagas disease

(2008) Juan M Bustamante et al.   NATURE MEDICINE

A mechanism for cross-resistance to nifurtimox and benznidazole in trypanosomes

(2008) Shane R. Wilkinson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA