Review
Biochemistry & Molecular Biology
Ruairidh Edwards, Ross Eaglesfield, Kostas Tokatlidis
Summary: The mitochondrial intermembrane space (IMS) is a narrow sub-mitochondrial compartment with diverse protein import mechanisms. IMS proteins do not require energy to cross the outer mitochondrial membrane, unlike import into the matrix or inner membrane. Many IMS proteins are associated with human diseases, highlighting the importance of understanding their normal function and localization in the IMS.
Article
Multidisciplinary Sciences
Qingqing Sun, Xueli Cao, Zihe Liu, Chuanjing An, Jinglei Hu, Yue Wang, Meiyu Qiao, Teng Gao, Wenzhen Cheng, Yi Zhang, Yue Feng, Hongbo Gao
Summary: Chloroplast division involves complex protein interactions, with PDV1 playing a crucial role in stabilizing PARC6. Under light conditions, reduction of a disulfide bond within PARC6 promotes its interaction with PDV1, ultimately leading to chloroplast division. Magnesium ions also enhance PARC6 dimerization, further contributing to the regulation of chloroplast division.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Qingqing Sun, Xueli Cao, Zihe Liu, Chuanjing An, Jinglei Hu, Yue Wang, Meiyu Qiao, Teng Gao, Wenzhen Cheng, Yi Zhang, Yue Feng, Hongbo Gao
Summary: Chloroplast division requires the coordination of protein complexes from stroma to cytosol. The interaction between PDV1 and PARC6, crucial for chloroplast division, is regulated by intramolecular disulfide bond formation in PARC6, which can be reduced by light exposure. Additionally, magnesium ions, which increase in chloroplasts upon light exposure, promote PARC6 dimerization.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biochemistry & Molecular Biology
Eleanor Dickson-Murray, Kenza Nedara, Nazanine Modjtahedi, Kostas Tokatlidis
Summary: Mitochondrial proteins, mostly encoded by nuclear genes, must be precisely targeted and sorted within mitochondria. Protein translocases facilitate this process, with the main import pathway for the intermembrane space chemically modifying imported proteins by introducing disulfide bonds. Mia40 is a key component in this pathway, regulated by redox processes, and plays a crucial role in trapping folded precursors in the intermembrane space.
Article
Cell Biology
Esra Peker, Konstantin Weiss, Jiyao Song, Christine Zarges, Sarah Gerlich, Volker Boehm, Aleksandra Trifunovic, Thomas Langer, Niels H. Gehring, Thomas Becker, Jan Riemer
Summary: Peker et al. discovered a two-step import pathway that allows proteins to be localized to both the matrix and IMS. Weak targeting signals enable proteins to form stabilizing disulfide bonds in the IMS before being imported into the matrix. This pathway enables the monitoring of import activity in both compartments. The study found that NDUFAF8, a factor involved in complex I assembly, follows this two-step import pathway, and its import is regulated by proteases to ensure proper function.
JOURNAL OF CELL BIOLOGY
(2023)
Review
Physiology
Kyle B. Heine, Hailey A. Parry, Wendy R. Hood
Summary: Our understanding of variation in mitochondrial performance is still limited. The structure of the inner mitochondrial membrane plays a crucial role in ATP production through oxidative phosphorylation. Changes in the morphology and density of the inner mitochondrial membrane can affect ATP synthesis. This review explores the association between ATP synthases, inner mitochondrial membrane density, and mitochondrial density in impacting ATP production by mitochondria, as well as the potential constraints on ATP production due to increased membrane density.
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zachary Spaulding, Indhujah Thevarajan, Lynn G. Schrag, Lejla Zubcevic, Anna Zolkiewska, Michal Zolkiewski
Summary: SKD3, a member of the AAA+ family of ATPases, plays important roles in 3-methylglutaconic aciduria and congenital neutropenia. It is upregulated in acute myeloid leukemia, contributing to drug resistance. Research has shown that SKD3 can form oligomers at high protein concentration or in the presence of nucleotides. The non-canonical structure of SKD3 may have functional significance.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Jakob D. Busch, Laura F. Fielden, Nikolaus Pfanner, Nils Wiedemann
Summary: Biogenesis of mitochondria involves the import of numerous precursor proteins across the mitochondrial membranes. Different import pathways exist, ranging from presequence-directed pathway to pathways using internal or carboxy-terminal targeting signals. Recent studies have revealed the structural organization of membrane-embedded preprotein translocases and their dynamic interactions with other cellular machineries. These insights provide valuable knowledge about the mechanisms of mitochondrial protein import.
Article
Multidisciplinary Sciences
Ivan Radin, Luise Kost, Uta Gey, Iris Steinebrunner, Gerhard Roedel
Summary: COX11 homologs play a crucial role in cellular redox homeostasis, mitigating the dangers of ROS and providing antioxidative protection. The gene is upregulated under oxidative stress and can reduce ROS levels effectively, suggesting a defensive role in protecting cells during heightened oxidative stress.
Article
Multidisciplinary Sciences
Sophie Kaspar, Christian Oertlin, Karolina Szczepanowska, Alexandra Kukat, Katharina Senft, Christina Lucas, Susanne Brodesser, Maria Hatzoglou, Ola Larsson, Ivan Topisirovic, Aleksandra Trifunovic
Summary: This study reveals the complex interplay between three transcription factors regulating mitochondrial stress response, in which CHOP acts as a regulator to attenuate prolonged ISR and prevent unfavorable metabolic alterations. The interaction between CHOP and C/EBP beta is necessary to adjust ATF4 levels and prevent overactivation of the ATF4-regulated transcriptional program, switching ISR from an acute to a chronic state.
Article
Biology
Barbara Uszczynska-Ratajczak, Sreedevi Sugunan, Monika Kwiatkowska, Maciej Migdal, Silvia Carbonell-Sala, Anna Sokol, Cecilia L. Winata, Agnieszka Chacinska
Summary: Most mitochondrial proteins are encoded by nuclear genes, synthesized in the cytosol, and targeted into the organelle. The presence of nuclear-encoded mRNA5 in the mitochondrial fraction confirms that large proteins with specific properties, like transmembrane domains, are predominantly encoded. Under proteostatic stress conditions, the population of transcripts on the mitochondrial surface is further restricted, allowing only the largest and most evolutionarily conserved proteins to be synthesized there.
LIFE SCIENCE ALLIANCE
(2022)
Article
Cell Biology
Marcel Morgenstern, Christian D. Peikert, Philipp Luebbert, Ida Suppanz, Cinzia Klemm, Oliver Alka, Conny Steiert, Nataliia Naumenko, Alexander Schendzielorz, Laura Melchionda, Wignand W. D. Muehlhaeuser, Bettina Knapp, Jakob D. Busch, Sebastian B. Stiller, Stefan Dannenmaier, Caroline Lindau, Mariya Licheva, Christopher Eickhorst, Riccardo Galbusera, Ralf M. Zerbes, Michael T. Ryan, Claudine Kraft, Vera Kozjak-Pavlovic, Friedel Drepper, Sven Dennerlein, Silke Oeljeklaus, Nikolaus Pfanner, Nils Wiedemann, Bettina Warscheid
Summary: Researchers have classified and identified human mitochondrial proteins, establishing a high-confidence mitochondrial proteome containing over 1,100 proteins, which account for 7% of cellular proteins and are linked to various diseases.
Article
Neurosciences
Avanti Gokhale, Chelsea E. Lee, Stephanie A. Zlatic, Amanda A. H. Freeman, Nicole Shearing, Cortnie Hartwig, Oluwaseun Ogunbona, Julia L. Bassell, Meghan E. Wynne, Erica Werner, Chongchong Xu, Zhexing Wen, Duc Duong, Nicholas T. Seyfried, Carrie E. Bearden, Viktor Janos Olah, Matthew J. M. Rowan, Jill R. Glausier, David A. Lewis, Victor Faundez
Summary: This study identified the importance of genes SLC25A1 and MRPL40, present in the deleted segment of 22q11.2 microdeletion syndrome, in maintaining mitochondrial ribosomal integrity and proteostasis, which are crucial for synaptic neurodevelopment and function. Fruit fly experiments demonstrated that mitochondrial ribosome function is essential for synaptic neurodevelopment, function, and behavior. Mitochondrial proteostasis perturbations, caused by genetic or environmental factors, may contribute to the pathogenesis of neurodevelopmental disorders.
JOURNAL OF NEUROSCIENCE
(2021)
Review
Physiology
Marcel G. Genge, Dejana Mokranjac
Summary: The majority of mitochondrial proteins are synthesized in the cytosol and contain specific mitochondrial targeting signals. These signals, such as presequences, help guide the proteins to the mitochondrial matrix with the assistance of TOM and TIM23 complexes in the outer and inner membranes. The TOM and TIM23 complexes interact in the intermembrane space, facilitating the transfer of presequences and precursor proteins from the outer to the inner mitochondrial membrane.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biology
Marcel G. Genge, Shalini Roy Chowdhury, Vit Dohnalek, Kaori Yunoki, Takashi Hirashima, Toshiya Endo, Pavel Dolezal, Dejana Mokranjac
Summary: This study reveals the coordination mechanism of translocation of proteins across mitochondrial membranes, and finds that both the core and PBD domains of Tim50 play essential roles in this process.
LIFE SCIENCE ALLIANCE
(2023)