期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 114, 期 32, 页码 E6546-E6555出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1703553114
关键词
flagellar distal end; trypanosome; flagella connector; axonemal capping structure; structure immunoprecipitation
资金
- Wellcome Trust [WT066839MA, 104627/Z/14/Z]
- Long Term Fellowship from European Molecular Biology Organization
- Czech Science Foundation (GA CR) Junior Project [16-26444Y]
- J. E. Purkyne Fellowship
- Wellcome Trust [104627/Z/14/Z] Funding Source: Wellcome Trust
The distal end of the eukaryotic flagellum/cilium is important for axonemal growth and signaling and has distinct biomechanical properties. Specific flagellum tip structures exist, yet their composition, dynamics, and functions are largely unknown. We used biochemical approaches to identify seven constituents of the flagella connector at the tip of an assembling trypanosome flagellum and three constituents of the axonemal capping structure at the tips of both assembling and mature flagella. Both tip structures contain evolutionarily conserved as well as kinetoplastid-specific proteins, and component assembly into the structures occurs very early during flagellum extension. Localization and functional studies reveal that the flagella connector membrane junction is attached to the tips of extending microtubules of the assembling flagellum by a kinesin-15 family member. On the opposite side, a kinetoplastidspecific kinesin facilitates attachment of the junction to the microtubules in the mature flagellum. Functional studies also suggest roles of several other components and the definition of subdomains in the tip structures.
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