2022年发表

Plasma circPTPN22 and its potential target microRNA-200a-3p as novel diagnostic biomarkers in systemic lupus erythematosus patients

作者: Hagar L. Mowafy, Maha M. Kotb, Kamal M. Hanna, Fatema T. Elgengehy, Lamiaa A. Madkour

发表期刊: The Egyptian Rheumatologist

2021年发表

Biotechnology of Nanostructures Micronutrients Vitamins for Human Health

Biotechnology Nanostructures Micronutrients Vitamins for Human

作者: Loutfy H. Madkour

发表期刊: J Biomed Res Environ Sci. 2021 May 22; 2(5): 358-371.

论文简介:

Nowadays, nanotechnology is used as a way to increase bioavailability and decrease the side effects of drugs and nutrients. Micronutrients and nutraceuticals such as vitamins, carotenoids, polyunsaturated fatty acids and polyphenols are classes of food ingredients that are essential for human health and well-being. These compounds are rarely added purely to the targeted food application but rather in encapsulated, solid, dry product forms with added functionalities such as improved stability, bioavailability or handling. Development of new strategies, like nanocarriers, that help to promote the access of neuroprotective molecules to the brain, is needed for providing more effective therapies for the disorders of the Central Nervous System (CNS). Polymer–lipid hybrid nanoparticles, encapsulating vitamin D3 and vitamin K2, with improved features in terms of stability, loading and mucoadhesiveness were produced for potential nutraceutical and pharmaceutical applications. Recently, nanoformulations that include nanovesicles, solid-lipid nanoparticles, nanostructured lipid carriers, nanoemulsions, and polymeric nanoparticles have shown promising outcomes in improving the efficacy and bioavailability of vitamin E. Active targeting of nanoparticles loaded with vitamin D to cancer cells.

2021年发表

Nucleic Acids (DNA/RNA) as Nanoparticles Structures for siRNA Delivery Medical Applications

(DNA/RNA) Nanoparticles Structures siRNA Delivery Medical Applications

作者: Loutfy H Madkour

发表期刊: Journal of Pharmaceutics and Drug Research JPDR, 4(2): 480-503

论文简介:

Engineered design of synthetic DNA/RNA molecules can generate pre-defined structures that can easily self-assemble to form nanoparticles with multiple functionalities. The identification and selection of highly potent siRNA sequences has already been accomplished for many gene targets, and the synthesis of siRNAs on a large scale has been achieved. The field of oligonucleotide-based nanotechnology for biomedical applications is just emerging, but will play an important role in the delivery of siRNA. In particular, oligonucleotide-based structural RNAi systems described in this chapter are promising as a new generation of gene delivery carriers for cancer therapy. RNA interference (RNAi) is a gene regulation mechanism initiated by RNA molecules that enables sequence-specific gene silencing by promoting degradation of specific mRNAs. Molecular therapy using small interfering RNA (siRNA) has shown great therapeutic potential for diseases caused by abnormal gene overexpression or mutation. The major challenges to application of siRNA therapeutics include the stability and effective delivery of siRNA in vivo. In this chapter, we discuss recent advances in nanoparticle-mediated siRNA delivery systems and the application of these systems in clinical trials for cancer therapy. Furthermore, we offer perspectives on future applications of siRNA therapeutics.

2021年发表

Imaging modalities delivery of RNAi therapeutics in cancer therapy and clinical applications

Imaging modalities delivery RNAi therapeutics cancer therapy

作者: Loutfy H Madkour*

发表期刊: J Radiol Oncol. 2021; 5: 005-034.

论文简介:

The RNA interference (RNAi) technique is a new modality for cancer therapy, and several candidates are being tested clinically. Nanotheranostics is a rapidly growing field combining disease diagnosis and therapy, which ultimately may add in the development of ‘personalized medicine’. Technologies on theranostic nanomedicines has been discussed. We designed and developed bioresponsive and fluorescent hyaluronic acid-iodixanol nanogels (HAI-NGs) for targeted X-ray computed tomography (CT) imaging and chemotherapy of MCF-7 human breast tumors. HAI-NGs were obtained with a small size of ca. 90 nm, bright green fluorescence and high serum stability from hyaluronic acid-cystamine-tetrazole and reductively degradable polyiodixanol-methacrylate via nanoprecipitation and a photo-click crosslinking reaction. This chapter presents an over view of the current status of translating the RNAi cancer therapeutics in the clinic, a brief description of the biological barriers in drug delivery, and the roles of imaging in aspects of administration route, systemic circulation, and cellular barriers for the clinical translation of RNAi cancer therapeutics, and with partial content for discussing the safety concerns. Finally, we focus on imaging-guided delivery of RNAi therapeutics in preclinical development, including the basic principles of different imaging modalities, and their advantages and limitations for biological imaging. With growing number of RNAi therapeutics entering the clinic, various imaging methods will play an important role in facilitating the translation of RNAi cancer therapeutics from bench to bedside.

2021年发表

Metal Nanoparticles and their Safety Processing in Functional Foods

Metal Nanoparticles safety processing functional foods

作者: Loutfy H. Madkour

发表期刊: Journal of Chemical Science and Chemical Engineering

论文简介:

A Safety of nanotechnology in food industries has been discussed. Different nanomaterials (NMs) such as nanopowders, nanotubes, nano-fibers, quantum dots, and metal and metal-oxide nanoparticles are globally produced in large quantities due to their broad applicability in food-related industries. We present some uses of nanoparticles in food and related industries and their possible side effects. The various aspects of nanoparticles and their impact on human exposure, safety, and environmental concerns have been discussed. ZnO NPs are listed as a kind of safe substance by the FDA. The safety of nanostructured synthetic amorphous silica (SAS) as a food additive (E 551) has been discussed. and has always been produced by the same two production methods. Bioavailability increases for iron of nanometer size. The characteristics of broad spectrum of activity against food pathogens of silver nanostructures …

2021年发表

Biotechnology of Nanostructures Micronutrients Vitamins for Human Health

Biotechnology Nanostructures Micronutrients Vitamins

作者: Loutfy H Madkour

发表期刊: J Biomed Res Environ Sci,

论文简介:

Nowadays, nanotechnology is used as a way to increase bioavailability and decrease the side effects of drugs and nutrients. Micronutrients and nutraceuticals such as vitamins, carotenoids, polyunsaturated fatty acids and polyphenols are classes of food ingredients that are essential for human health and well-being. These compounds are rarely added purely to the targeted food application but rather in encapsulated, solid, dry product forms with added functionalities such as improved stability, bioavailability or handling. Development of new strategies, like nanocarriers, that help to promote the access of neuroprotective molecules to the brain, is needed for providing more effective therapies for the disorders of the Central Nervous System (CNS). Polymer–lipid hybrid nanoparticles, encapsulating vitamin D3 and vitamin K2, with improved features in terms of stability, loading and mucoadhesiveness were produced for potential nutraceutical and pharmaceutical applications. Recently, nanoformulations that include nanovesicles, solid-lipid nanoparticles, nanostructured lipid carriers, nanoemulsions, and polymeric nanoparticles have shown promising outcomes in improving the effi cacy and bioavailability of vitamin E. Active targeting of nanoparticles loaded with vitamin D to cancer cells

2021年发表

Recent Targeted of siRNA Delivery Vehicles for Cancer Therapy

Recent Targeted siRNA Delivery Vehicles Cancer Therapy

作者: Loutfy H Madkour

发表期刊: Biomed J Sci & Tech Res 34(4)-2021. BJSTR. MS.ID.005577.

论文简介:

Recent progress in RNA biology has broadened the scope of therapeutic targets of RNA drugs for cancer therapy. However, RNA drugs, typically small interfering RNAs (siRNAs), are rapidly degraded by RNases and filtrated in the kidney, thereby requiring a delivery vehicle for efficient transport to the target cells. To date, various delivery formulations have been developed from cationic lipids, polymers, and/or inorganic nanoparticles for systemic delivery of siRNA to solid tumors. This research article describes the current status of clinical trials related to siRNA-based cancer therapy, as well as the remaining issues that need to be overcome to establish a successful therapy. It, then introduces various promising design strategies of delivery vehicles for stable and targeted siRNA delivery, including the prospects for future design. The current major strategies to design delivery vehicles for systemic siRNA delivery involve the construction of multimolecular assemblies from more than dozens of monomer components, including siRNA. The success of RNAi-based cancer therapy is closely associated with tumor biology as well as architecture of delivery vehicles. Tumor cell plasticity evokes a resistance mechanism against clinical treatments, and cancer stem cells are gradually being identified as the root of cancer recurrence. New target RNA genes should be discovered to increase apoptosis in cancer cells and simultaneously reduce side effects in normal and healthy cells. Multidisciplinary research studies will guide the development of highly effective and safer RNAi-based drugs in clinical trials. Keywords: RNAi; siRNA Delivery; Polyion Complex Micelle; Systemic Administration; Cancer Therapy

2021年发表

Chemical, Structural, Advances and Hurdles to Clinical Translation of RNAi Therapeutics

Chemical Structural Advances and Hurdles RNAi Therapeutics

作者: Loutfy H Madkour

发表期刊: Journal of Radiology and Medical Imaging, Publisher: MedDocs Publishers LLC

论文简介:

Abstract RNAi can wipe out the disease-causing proteins from being translated. Now RNAi technique has become a powerful tool for basic research to selectively knock down gene expression in vitro and in vivo. At the same time, both scientific and industrial communities started to develop RNAi therapeutics as the next class of drugs for treating a variety of genetic disorders, such as cancer and other diseases that are particularly hard to be addressed by current treatment strategies. Small interfering RNA (siRNA), a 21–23 nt double-stranded RNA responsible for post-transcriptional gene silencing, has attracted great interests as promising genomic drugs, due to its strong ability to silence target genes in a sequence-specific manner. In order to develop siRNAs as therapeutic agents for cancer treatment, delivery strategies for siRNA must be carefully designed and potential gene targets carefully selected for optimal anti-cancer effects. In this review, various modifications and delivery strategies for siRNA delivery are discussed. In addition, we present current thinking on target gene selection in major tumor types.

2021年发表

A Review: Metal Nanoparticles and their Safety Processing in Functional Foods

Metal Nanoparticles Safety Processing Functional Foods

作者: Loutfy H. Madkour

发表期刊: Journal of Chemical Science and Chemical Engineering 1(3): 18-36.

论文简介:

Abstract Safety of nanotechnology in food industries has been discussed. Different nonmaterial’s (NMs) such as nanopowders, nanotubes, nano-fibers, quantum dots, and metal and metal-oxide nanoparticles are globally produced in large quantities due to their broad applicability in food-related industries. We present some uses of nanoparticles in food and related industries and their possible side effects. The various aspects of nanoparticles and their impact on human exposure, safety, and environmental concerns have been discussed. ZnO NPs are listed as a kind of safe substance by the FDA. The safety of nanostructured synthetic amorphous silica (SAS) as a food additive (E 551) has been discussed. and has always been produced by the same two production methods. Bioavailability increases for iron of nanometer size. The characteristics of broad spectrum of activity against food pathogens of silver nanostructures gives an insight for their potential applicability in incorporation of food packaging materials and antimicrobials for stored fruits and foods. Keywords: Food-processing industry; Food safety; Nanostructured; Silicon dioxide; Silver nanostructures; ZnO NPs; Antimicrobial activity

2021年发表

Pharmacokinetics, Bio Distribution and Therapeutic Applications of Recently-Developed siRNA and DNA Repair Genes Recurrence

RNA interference Small interfering RNA Cancer Delivery Pharmacokinetics;

作者: Loutfy H. Madkour

发表期刊: Austin J Anal Pharm Chem. 2021; 8(2): 1135.

论文简介:

Up regulation of cell cycle-regulating and DNA repair genes appears to have a negative impact on recurrence-free survival in patients with papillary thyroid cancer. Furthermore, recurrence is associated with thyroid dedifferentiation. Most cases address local applications or diseases in the filtering organs, reflecting remaining challenges in systemic delivery of siRNA. Small Interfering RNA (siRNA) is a promising drug candidate, expected to have broad therapeutic potentials toward various diseases including viral infections and cancer. With recent advances in bio conjugate chemistry and carrier technology, several siRNA-based drugs have advanced to clinical trials. The difficulty in siRNA delivery is in large part due to poor circulation stability and unfavorable pharmacokinetics and bio distribution profiles of siRNA. In this research we describe the pharmacokinetics and bio distribution of siRNA Nano medicines, focusing on those reported in the past 5 years, and their pharmacological effects in selected disease models such as hepatocellular carcinoma, liver infections, and respiratory diseases. The examples discussed here will provide an insight into the current status of the art and unmet needs in siRNA delivery.