Article
Biochemistry & Molecular Biology
Guisela Fernandez-Espinosa, Elvira Orduna-Hospital, Ana Boned-Murillo, Maria Dolores Diaz-Barreda, Ana Sanchez-Cano, Maria Sopena-Pinilla, Isabel Pinilla
Summary: This study aimed to investigate the choroidal thickness in patients with moderate diabetic retinopathy and its correlation with retinal thickness compared to healthy subjects. The results showed that retinal thickness was higher in certain areas in the diabetic group, but choroidal thickness did not differ between the groups. Additionally, age was found to have an impact on retinal thickness and choroidal thickness.
Article
Biochemical Research Methods
Xuehua Wang, Rui Li, Junyan Chen, Dingan Han, Mingyi Wang, Honglian Xiong, Wenzheng Ding, Yixu Zheng, Ke Xiong, Yaguang Zeng
Summary: A deep learning model called CVI-Net is proposed for automatic segmentation of the choroid layer and its vessels in OCT scans. It quantifies clinical parameters to determine structural and vascular changes in the choroid with the progression of DR severity. The results show high accuracy and a significant negative correlation between CVI and DR severity.
JOURNAL OF BIOPHOTONICS
(2023)
Review
Medicine, General & Internal
Jikuan Jiang, Jingyuan Liu, Jia Yang, Bing Jiang
Summary: This study examined the differences in subfoveal choroidal thickness and choroidal vascularity index in diabetic eyes with and without retinopathy. The results showed that patients with diabetic retinopathy had thicker SFCT and lower CVI values, suggesting these parameters could be useful in monitoring the onset of retinopathy.
FRONTIERS IN MEDICINE
(2022)
Article
Medicine, General & Internal
Huiping Yao, Zijian Yang, Yu Cheng, Xi Shen
Summary: This study investigated the changes in macular status and choroidal thickness following phacoemulsification in patients with mild to moderate nonproliferative diabetic retinopathy. The results showed that the increase in SCP-VD, MT, and CT after surgery was significantly greater in diabetic retinopathy patients compared to the control group.
FRONTIERS IN MEDICINE
(2023)
Article
Medicine, General & Internal
Tian Huang, Xiaoli Li, Jie Xie, Liang Zhang, Guanrong Zhang, Aiping Zhang, Xiangting Chen, Ying Cui, Qianli Meng
Summary: The study assessed long-term retinal microvascular, neural, and choroidal changes following panretinal photocoagulation in patients with severe nonproliferative diabetic retinopathy and proliferative diabetic retinopathy. The findings showed no significant differences in visual acuity, vessel density, and choroidal thickness post-PRP, but indicated increased macular thickness and changes in RNFL and GCC thickness. The results suggest that PRP may help prevent retinal neurovascular and choroidal damage in diabetic retinopathy patients.
FRONTIERS IN MEDICINE
(2021)
Article
Medicine, General & Internal
Hafsa Hassan, Alyscia Cheema, Muhammad Ali Tahir, Hina Nasreen Nawaz
Summary: This study found that the central choroidal thickness as well as average choroidal thickness were significantly reduced in eyes with diabetic retinopathy compared to non-diabetic healthy eyes, indicating that changes in the choroid may be a possible route in the pathogenesis of diabetic retinopathy.
PAKISTAN JOURNAL OF MEDICAL SCIENCES
(2022)
Review
Medicine, General & Internal
Rui Xie, Bingjie Qiu, Jay Chhablani, Xinyuan Zhang
Summary: The advancement of optical coherence tomography (OCT) technology has greatly improved the analysis of choroidal morphology and physiology, especially with the use of enhanced depth imaging OCT and swept-source OCT. This has led to a better understanding of choroidal disorders in retinal and choroidal diseases.
FRONTIERS IN MEDICINE
(2021)
Article
Medicine, General & Internal
Xue Bai, Rui Hua
Summary: The study found that fluorescein angiography had a higher accuracy in detecting the severity of diabetic retinopathy and macular leakages compared to optical coherence tomography. Central foveal thickness is not a sensitive parameter for detecting latent DMEs.
FRONTIERS IN MEDICINE
(2021)
Article
Ophthalmology
Xia Gong, Wei Wang, Kun Xiong, Lanhua Wang, Wangting Li, Yuting Li, Meng Yuan, Xiao Guo, Shaochong Zhang, Xiaoling Liang, Hua Liu, Wenyong Huang
Summary: This study evaluated the role of the peripapillary retinal nerve fiber layer (pRNFL) and peripapillary choroidal thickness (pCT) in the development and progression of diabetic retinopathy (DR). The results showed that thinning of pRNFL and pCT were strongly associated with increased risk of incident DR. Assessment of both metrics improved the risk assessment for DR incidences.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Medicine, General & Internal
Qian Ren, Hua Yu, Zhaohui Sun, Li Li
Summary: The study aimed to evaluate the changes in choroidal thickness in patients with stage IV-V diabetic retinopathy (DR) treated with Panretinal Photocoagulation (PRP) or its combination with anti-vascular endothelial growth factor (VEGF) therapy using optical coherence tomography angiography (OCTA). Results showed that choroidal thickness increased in the short term but gradually decreased in the long term after treatment. OCTA provides an effective evaluation of choroidal thickness in patients with proliferative DR, aiding in treatment selection and efficacy determination.
PAKISTAN JOURNAL OF MEDICAL SCIENCES
(2021)
Article
Medicine, General & Internal
Hui Wang, Xuhui Liu, Xiaofeng Hu, Hua Xin, Han Bao, Shuo Yang
Summary: This study evaluated the changes in fundus vascular density and micromorphological structure in different stages of diabetic retinopathy (DR), as well as the correlation between fundus blood flow and DR severity. Patients with diabetes mellitus (DM) showed significant correlations between the densities and perfusion of retinal capillary plexuses and the severity of DR. OCTA parameters have high sensitivity and specificity in distinguishing DR from healthy eyes.
FRONTIERS IN MEDICINE
(2023)
Review
Medicine, General & Internal
Sara Vaz-Pereira, Tiago Morais-Sarmento, Michael Engelbert
Summary: This review discusses recent developments in the use of optical coherence tomography (OCT) for proliferative diabetic retinopathy (PDR), focusing on the higher detection rate of neovascularization and improving retinal imaging quality with widefield-OCT and OCT-angiography (OCTA). Additionally, studies have advanced in analyzing retinal nonperfusion areas (NPAs) and the impact of PDR treatment on NPAs and vascular density. Ongoing technological developments in artificial intelligence and deep learning are enhancing imaging protocols for managing PDR without the need for invasive conventional fluorescein angiography.
Article
Ophthalmology
Ling-Yun Ma, Ao Rong, Yi Jiang, Shu-Ya Deng
Summary: This study compared the changes in retinal and choroid thickness in diabetic patients after FLACS and PE surgery, and found that FLACS group had advantages in reducing EPT, achieving good vision earlier, and promoting faster recovery of retinal and choroidal thickness. Baseline CMT was a significant predictor of CMT changes in the early period after FLACS.
OPHTHALMOLOGY AND THERAPY
(2021)
Article
Pharmacology & Pharmacy
Maria Consiglia Trotta, Carlo Gesualdo, Chiara Bianca Maria Platania, Domenico De Robertis, Mauro Giordano, Francesca Simonelli, Michele D'Amico, Filippo Drago, Claudio Bucolo, Settimio Rossi
Summary: This study identified five miRNAs associated with stages of diabetic retinopathy, validating them as potential prognostic biomarkers and pharmacological targets. Additionally, optical coherence tomography (OCT) assessment showed an increase in the number of hyperreflective spots (HRS) with worsening of DR stages.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Medicine, General & Internal
Qingquan Wei, Wei Qiu, Qing Liu, Yanyun Jiang
Summary: This study examined the changes in macular thickness (MT) and potential risk factors in normal individuals, patients without obvious diabetic retinopathy (NDR), and patients with nonproliferative diabetic retinopathy (NPDR). The results showed significant differences in MT in the central and inner ring regions between the NPDR group, NDR group, and control group. Diabetic duration, total cholesterol, triglycerides, systolic pressure, and diastolic pressure were positively correlated with MT in the central region.
INTERNATIONAL JOURNAL OF GENERAL MEDICINE
(2022)
Review
Biotechnology & Applied Microbiology
Antonia Lichtenegger, Bernhard Baumann, Yoshiaki Yasuno
Summary: The zebrafish is an established vertebrate model in biomedical research, especially for high-throughput studies. Recently, optical coherence tomography (OCT) has been widely used in zebrafish research. This review article summarizes the relevant literature and provides an outlook for this emerging field.
BIOENGINEERING-BASEL
(2023)
Article
Biochemical Research Methods
Kiriko Tomita, Shuichi Makita, Naoki Fukutake, Rion Morishita, Ibrahim Abd El-Sadek, Pradipta Mukherjee, Antonia Lichtenegger, Junya Tamaoki, Lixuan Bian, Makoto Kobayashi, Tomoko Mori, Satoshi Matsusaka, Yoshiaki Yasuno
Summary: This article presents a new formulation for the lateral imaging process of point-scanning optical coherence tomography (OCT) and introduces a new differential contrast method based on this formulation. The formulation utilizes a mathematical sample model called the dispersed scatterer model (DSM), where the sample is represented as a material with slowly varying refractive index and randomly distributed scatterers. It is demonstrated that the formulation represents OCT image and speckle as separate mathematical quantities. The new differential contrast method utilizes complex signal processing of OCT images and is validated through experiments on in vivo and in vitro samples.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Biochemical Research Methods
Rion Morishita, Toshio Suzuki, Pradipta Mukherjee, Ibrahim Abd El-Sadek, Yiheng Lim, Antonia Lichtenegger, Shuichi Makita, Kiriko Tomita, Yuki Yamamoto, Tetsuharu Nagamoto, Yoshiaki Yasuno
Summary: We utilized 3D dynamic optical coherence tomography (DOCT) to observe the intratissue and intracellular activities of hiPSCs-derived alveolar organoids in normal and fibrosis models. The DOCT images revealed cystic structures surrounded by high-intensity borders, potentially representing alveoli with highly dynamic epithelium, as well as mesh-like structures, possibly fibroblasts. Furthermore, the images displayed abnormal repair of the alveolar epithelium.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Engineering, Electrical & Electronic
Yoshiaki Yasuno
Summary: Jones-matrix optical coherence tomography (JM-OCT) is an extension of polarization-sensitive OCT that provides multiple optical contrasts for biological and clinical samples. It measures the spatial and temporal distribution of the Jones matrix in the sample to compute various contrasts, including intensity, phase retardation, polarization uniformity, diattenuation, signal attenuation, scatterer density, Doppler OCT, OCT angiography, and dynamic OCT. JM-OCT is a generalized version of OCT as it measures the generalized form of sample information, i.e., the Jones matrix sequence. This review summarizes the concept, principles, implementation, processing, and applications of JM-OCT, including noise correction, quantity estimation, and self-calibration.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2023)
Article
Biochemical Research Methods
Lida Zhu, Shuichi Makita, Junya Tamaoki, Antonia Lichtenegger, Yiheng Lim, Yiqiang Zhu, Makoto Kobayashi, Yoshiaki Yasuno
Summary: This study proposes a method called multi-focus averaging (MFA) to improve the image contrast of optical coherence tomography (OCT) by suppressing multiple-scattering signals. The MFA method captures multiple OCT volumes with various focal positions and averages them in complex form, effectively reducing multiple-scattering and enhancing image contrast.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Biochemical Research Methods
Toshihiro Mino, Yoshikiyo Moriguchi, Masato Tamura, Akiko Matsumoto, Atsushi Kubota, Masahiro Akiba, Yunchan Hwang, Shuichi Makita, Yoshiaki Yasuno, Hiroshi Enaida, James G. Fujimoto, Zhenguo Wang
Summary: This study proposes a novel scanning protocol called ammonite scan for widefield optical coherence tomography angiography (OCTA) and relative retinal blood flow velocity imaging in the human retina using variable interscan time analysis (VISTA). The protocol achieved an interscan time of 1.28 ms through a repeated circle scan with a 400 kHz swept-source. The study also eliminated image artifacts caused by eye movement through extra motion-correction processing and explored the microvascular flow parameter in the healthy human eye using the VISTA image.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Multidisciplinary Sciences
Pradipta Mukherjee, Shinichi Fukuda, Donny Lukmanto, Thi Hang Tran, Kosuke Okada, Shuichi Makita, Ibrahim Abd El-Sadek, Yiheng Lim, Yoshiaki Yasuno
Summary: This paper presents label-free functional activity imaging of renal tubules in normal and obstructed mouse kidney models using three-dimensional dynamic optical coherence tomography (OCT). The study found that the structure of renal tubules changed and a high dynamics appearance was observed in the perirenal cortex region in the obstructed kidneys. These findings provide a new tool for studying kidney function during kidney diseases.
SCIENTIFIC REPORTS
(2023)
Proceedings Paper
Engineering, Biomedical
Yusong Liu, Shuichi Makita, Masahiro Miura, Takuya Iwasaki, Shinnosuke Azuma, Toshihiro Mino, Tatsuo Yamaguchi, Yoshiaki Yasuno
Summary: A U-Net shape model was used to synthesize retinal DOPU from OCT with OCTA images. The synthesized DOPU showed high recall and precision for healthy structure, RPE elevation, and RPE thickening. However, improvement is needed for synthesizing RPE defect and hyperreflective retina foci.
OPHTHALMIC TECHNOLOGIES XXXIII
(2023)
Proceedings Paper
Computer Science, Artificial Intelligence
Lida Zhu, Shuichi Makita, Yiheng Lim, Junya Tamaoki, Yiqiang Zhu, Makoto Kobayashi, Yoshiaki Yasuno
Summary: Optical coherence tomography (OCT) is used to investigate tissue optical properties, while polarization-sensitive OCT (PS-OCT) provides additional contrasts. Multiple scattering (MS) in biological samples hampers OCT and PS-OCT measurements. Various methods, including wavefront shaping, have been proposed to tackle MS. This study presents a method called multi-focus averaging (MFA) to suppress MS and improve image contrast in deep regions. MFA effectively mitigates artifacts caused by MS and improves the accuracy of polarization-sensitive measurements.
OPTICAL COHERENCE TOMOGRAPHY AND COHERENCE DOMAIN OPTICAL METHODS IN BIOMEDICINE XXVII
(2023)
Proceedings Paper
Computer Science, Artificial Intelligence
Yiqiang Zhu, Lida Zhu, Shuichi Makita, Yiheng Lim, Yoshiaki Yasuno
Summary: In this study, a B-scan-wise-multi-focus averaging (B-MFA) method was proposed to suppress multiple scattering noise in optical coherence tomography. This method utilizes multiple B-scans with different focus positions, which are computationally refocused and complex averaged. The results demonstrate the ability of B-MFA in suppressing multiple scattering noise.
OPTICAL COHERENCE TOMOGRAPHY AND COHERENCE DOMAIN OPTICAL METHODS IN BIOMEDICINE XXVII
(2023)
Article
Biochemical Research Methods
Shuichi Makita, Masahiro Miura, Shinnoshuke Azuma, Toshihiro Mino, Yoshiaki Yasuno
Summary: Degree of polarization uniformity (DOPU) imaging obtained by polarization-sensitive optical coherence tomography (PS-OCT) has the potential to provide biomarkers for retinal diseases. A neural network-based approach is presented to estimate the DOPU from standard OCT images. DOPU images are synthesized by the neural network and compared with ground truth DOPU images to evaluate the clinical findings. The proposed method demonstrates the potential of extending the features of retinal non-PS OCT.
BIOMEDICAL OPTICS EXPRESS
(2023)
Proceedings Paper
Engineering, Biomedical
Shuichi Makita, Lida Zhu, Kensuke Oikawa, Yoshiaki Yasuno
Summary: Post-signal-processing techniques in optical coherence tomography (OCT), such as refocusing and digital aberration correction, aim to restore degraded spatial resolution caused by optical aberrations. Recent studies have shown promising results with Lissajous-pattern-based probe beam scanning and motion correction algorithm, as well as a Lissajous-cycle-wise (LCW) digital refocus algorithm.
OPTICAL COHERENCE TOMOGRAPHY AND COHERENCE DOMAIN OPTICAL METHODS IN BIOMEDICINE XXVI
(2022)
