Journal
BIOCHEMICAL PHARMACOLOGY
Volume 186, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bcp.2021.114473
Keywords
Diabetic retinopathy; miRNA; Optical coherence tomography; TNF-alpha
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Funding
- Italian MIUR [PRIN 2017TSHBXZ]
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This study identified five miRNAs associated with stages of diabetic retinopathy, validating them as potential prognostic biomarkers and pharmacological targets. Additionally, optical coherence tomography (OCT) assessment showed an increase in the number of hyperreflective spots (HRS) with worsening of DR stages.
In this study we analyzed the expression of circulating miRNAs, in the serum of diabetic retinopathy (DR) patients. Five miRNAs (hsa-miR-195-5p, hsa-miR-20a-5p, hsa-miR-20b-5p, hsa-miR-27b-3p and hsa-miR-451a) were validated as biomarkers for stratification of DR stages, from the early non-proliferative (NPDR) to the late proliferative (PDR) phase. Furthermore, circulating levels of these miRNAs correlated with retinal hyperreflective spots (HRS), assessed by optical coherence tomography (OCT). The number of HRS increased with worsening of DR stages. On the contrary, no significant vascular density differences between NPDR and PDR patients were detected by angio-OCT (OCTA). A post-hoc bioinformatics analysis associated these five miRNAs to target genes belonging to the ?Tumor Necrosis Factor alfa signaling? pathway, and several molecules were predicted to modify miRNAs expression. In conclusion, correlation between specific circulating miRNAs and intraretinal hyper-reflective spots was demonstrated, confirming that these miRNAs were validated as prognostic biomarkers, and also as potential pharmacological targets, warranting further clinical evaluation to explore novel therapeutics for diabetic retinopathy.
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