Article
Microbiology
Haruka Ishihara, Kohei Ogura, Van An Nguyen, Tohru Miyohi-Akiyama, Shigefumi Okamoto, Norihiko Takemoto
Summary: This study identified the complete genomic sequences of Lancefield group A SDSE strains, revealing a group-specific conserved region that can be targeted for differentiation through PCR. The growth of GA-SDSE was suppressed in the presence of other SDSE and S. pyogenes, potentially explaining the low frequency of isolation of GA-SDSE strains.
JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Medicine, General & Internal
Tadayoshi Ikebe, Hitoshi Otsuka, Kazuki Chiba, Yu Kazawa, Takahiro Yamaguchi, Rumi Okuno, Yoshimi Date, Mari Sasaki, Junko Isobe, Makoto Ohnishi, Yukihiro Akeda
Summary: This study identified csrS/csrR and srrG gene mutations that are associated with increased virulence gene expression in STSS-SDSE isolates. Strains carrying these mutations exhibited enhanced pathogenicity in mice. The higher frequency of mutations observed in STSS-SDSE isolates highlights their importance in STSS.
Article
Infectious Diseases
Guillem Lopez de Egea, Aida Gonzalez-Diaz, Gerard Guedon, Julie Lao, Damaris Berbel, Antonio Casabella, Jose Maria Marimon, Emilia Cercenado, Lucia Fernandez-Delgado, Helene Chiapello, Thomas Lacroix, Maria Angeles Dominguez, Nathalie Leblond-Bourget, Carmen Ardanuy
Summary: In this study, we investigated the genes and mobile genetic elements associated with tetracycline resistance in Streptococcus dysgalactiae subsp. equisimilis (SDSE). Out of 84 SDSE isolates, 28.5% were tetracycline resistant and carried tet(M), tet(W), or both tet(L) and tet(W) genes. The tet(M) gene was found in ICEs of the Tn916-family and a new IME. The new IME is a major cause of tetracycline resistance in invasive SDSE in our settings.
Article
Infectious Diseases
Viivi Nevanlinna, Reetta Huttunen, Janne Aittoniemi, Tiina Luukkaala, Sari Rantala
Summary: This study aimed to investigate the risk factors associated with S. dysgalactiae subsp. equisimilis bacteremia. Using the Finhealth study data in Finland, it was found that obesity, diabetes, and coronary artery disease were associated with an increased risk of S. dysgalactiae subsp. equisimilis bacteremia.
BMC INFECTIOUS DISEASES
(2023)
Article
Fisheries
Jiao Wang, Yang Feng, Zhenyang Qin, Yi Geng, Xiaoli Huang, Ping Ouyang, Defang Chen, Hongrui Guo, Zhicai Zuo, Huidan Deng, Jing Fang, Weimin Lai
Summary: This study reports for the first time that Streptococcus dysgalactiae is a virulent pathogen in channel catfish in China. The characteristics and etiology of the disease were described, and the pathogenicity of the isolated strain was confirmed. The findings of this study also provide important information on the range of hosts affected by S. dysgalactiae.
AQUACULTURE REPORTS
(2022)
Article
Medicine, General & Internal
Hirokazu Toyoshima, Motoaki Tanigawa, Chiaki Ishiguro, Hiroyuki Tanaka, Yuki Nakanishi, Shigetoshi Sakabe
Summary: We report the first case of cervical osteomyelitis caused by septic atlantoaxial arthritis due to SDSE in an immunocompetent individual, presenting with progressive atlantoaxial subluxation. We also provide a review of the relevant literature.
Article
Microbiology
Cinthia Alves-Barroco, Joao Caco, Catarina Roma-Rodrigues, Alexandra R. Fernandes, Ricardo Bexiga, Manuela Oliveira, Lelia Chambel, Rogerio Tenreiro, Rosario Mato, Ilda Santos-Sanches
Summary: The study suggests that there is a niche expansion for Streptococcus dysgalactiae subsp. dysgalactiae (SDSD) into human infections, possibly due to the acquisition of virulence genes. Genetic relationships between human SDSD, SDSE, and bovine SDSD were analyzed, showing closer relationships between human strains. The presence of the CRISPR/Cas IIA system among bovine SDSD isolates and the maintenance of virulence genes over time indicate potential activities and adaptations within the subspecies.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Infectious Diseases
Keishin Sunagawa, Takuya Shirafuji, Guodong Sun, Rina Arai, Hiroki Azuma, Tohru Miyoshi-Akiyama, Harutaka Katano
Summary: This is the first case report of intra-familial transmission of Streptococcus dysgalactiae subsp. equisimilis.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2022)
Review
Infectious Diseases
Chen-Hong He, Shu-Fan Feng, Shu-Xin Chen, Deng-Ke Han, Tian-Rong He, Jian-Wei Cao, Hui-Qiang Mai
Summary: This is a case of human infection with SDSD, which is believed to be limited to animals. The patient experienced muscle pain and weakness, followed by sore throat, headache, and fever. After treatment, the patient's condition greatly improved and fully recovered within a month.
BMC INFECTIOUS DISEASES
(2023)
Article
Agriculture, Dairy & Animal Science
Anne Elisabeth Reetz, Etienne Aubry, Kinga Teske, Andreas Ochs, Lennard Epping, Torsten Semmler, Antina Luebke-Becker, Marcus Fulde, Lars Mundhenk
Summary: Movement disorders in rhinoceroses can be caused by lesions affecting the feet and interdigital space. This case study focuses on a 3-year-old male Greater one-horned rhinoceros that developed a severe movement disorder of the right hind limb and eventually died. The pathological analysis revealed a retroperitoneal abscess and chronic thrombosis of the right iliac artery with the presence of Streptococcus dysgalactiae subspecies equisimilis. The same bacteria was also found in the vaginal swab of the dam. This suggests that the list of differential diagnoses for lameness in rhinoceroses should consider causes beyond extremities.
Article
Agriculture, Dairy & Animal Science
M. Smistad, H. Kaspersen, F. Franklin-Alming, C. Wolff, L. Solverod, D. Porcellato, E. Trettenes, H. J. Jorgensen
Summary: This study aimed to investigate risk factors, sources, and genetic diversity of Streptococcus dysgalactiae ssp. dysgalactiae (SDSD) infection in Norwegian bovine dairy herds. The study found that closed flooring and rubber mats were risk factors for high incidence of SDSD intramammary infections (SDSD-IMI), while automatic milking systems were associated with lower incidence. External sources such as wounds and skin of the animals and the cubicle bases were frequently positive for SDSD. Eight multilocus sequence types (ST) were identified among the SDSD isolates, with one ST (ST453) being widely distributed in 15 out of 27 herds.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Infectious Diseases
Yasuto Fukushima, Yoshiko Takayama, Haruno Yoshida, Mieko Goto, Yuzo Tsuyuki, Takashi Takahashi
Summary: This study evaluated the feasibility of using CRISPR array-based genogrouping for the identification of Streptococcus bacteria and compared it to traditional typing methods. The results showed that CRISPR array genogrouping is a reliable method for determining the genetic relationship of Streptococcus isolates.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2022)
Article
Microbiology
Andreas Itzek, Victoria Weissbach, David Meintrup, Beate Riess, Mark van der Linden, Stefan Borgmann
Summary: SDSE is an important beta-hemolytic pathogen, and this study reveals an increasing infection burden for the German population, mainly driven by a mutation-driven outbreak of the stG62647 strain. Men are more susceptible to infection, particularly those with the stG62647 strain, while women with non-stG62647 strains are younger. Increasing age is a general risk factor for invasive SDSE infections.
Article
Veterinary Sciences
Carla Paola Bustos, Gabriela Retamar, Romina Leiva, Sara Frosth, Ana Ivanissevich, Maria Eugenia Demarchi, Saoirse Walsh, Lars Frykberg, Bengt Guss, Maria Mesplet, Andrew Waller
Summary: This study investigated the genotypic and phenotypic properties of Streptococcus dysgalactiae subsp. equisimilis (Sde) strain related to equine neonatal mastitis. It identified a novel genotype and horse-specific Sde strain as the causative agent of this condition.
JOURNAL OF EQUINE VETERINARY SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Sara Taylor, Deonne Walther, Deepani D. Fernando, Pearl Swe-Kay, Katja Fischer
Summary: This study investigates the antimicrobial potential of current and emerging scabicides and finds that both abametapir and manuka oil have antimicrobial properties against common scabies-associated bacteria. The current scabicide ivermectin also shows some antimicrobial activity.
Article
Microbiology
Ahmed Tabbabi, Shinya Watanabe, Daiki Mizushima, Abraham G. Caceres, Eduardo A. Gomez, Daisuke S. Yamamoto, Longzhu Cui, Yoshihisa Hashiguchi, Hirotomo Kato
Summary: Variations in the gut microbial content of Lutzomyia ayacuchensis populations from Ecuador and Peru were investigated, showing differences in bacterial and fungal compositions. While specific bacterial genera were found in different regions, there was also overlap in microbial communities. The study suggests that the ecological diversity of sand flies in Peru and Ecuador may influence their susceptibility to Leishmania infection through variations in gut microbiota.
Article
Microbiology
Pourya Gholizadeh, Mohammad Aghazadeh, Reza Ghotaslou, Mohammad Ahangarzadeh Rezaee, Tahereh Pirzadeh, Longzhu Cui, Shinya Watanabe, Hadi Feizi, Hiva Kadkhoda, Hossein Samadi Kafil
Summary: The study showed a negative correlation between CRISPR-Cas loci and antibiotic resistance in Enterococcus faecalis isolates, indicating that CRISPR-Cas systems may contribute to limiting the development and evolution of antibiotic resistance in bacteria.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2021)
Review
Biochemistry & Molecular Biology
Srivani Veeranarayanan, Aa Haeruman Azam, Kotaro Kiga, Shinya Watanabe, Longzhu Cui
Summary: Cancer, especially solid tumors, presents challenges in modern medicine due to its unique characteristics and variations in microenvironments. Traditional treatment options are limited and ineffective, calling for tailored and personalized approaches. Bacteriophage vectors offer potential for improved tumor theragnostics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Dai Akine, Teppei Sasahara, Kotaro Kiga, Ryusuke Ae, Koki Kosami, Akio Yoshimura, Yoshinari Kubota, Kazumasa Sasaki, Yumiko Kimura, Masanori Ogawa, Shinya Watanabe, Yuji Morisawa, Longzhu Cui
Summary: A high prevalence of extended-spectrum beta-lactamase-producing Enterobacterales (ESBL-PE) in geriatric long-term care facilities may require monitoring. This study analyzed ESBL-causative gene types and antimicrobial susceptibility in ESBL-PE strains from residents in Japanese g-LTCFs. The majority of strains contained bla(CTX-M) group genes and exhibited differences in antimicrobial susceptibility across facilities. Treatment strategies should take into account the specific antimicrobial susceptibility profiles of each g-LTCF.
Article
Microbiology
Yusuke Taki, Shinya Watanabe, Yusuke Sato'o, Xin-Ee Tan, Hisaya K. Ono, Kotaro Kiga, Yoshifumi Aiba, Teppei Sasahara, Aa Haeruman Azam, Kanate Thitiananpakorn, Srivani Veeranarayanan, Feng-Yu Li, Yuancheng Zhang, Tomofumi Kawaguchi, Sarah Hossain, Dong-Liang Maniruzzaman, Dong-Liang Hu, Longzhu Cui
Summary: In patients with non-menstrual toxic shock syndrome, human serum can induce the production of toxic shock syndrome toxin-1 (TSST-1) in Staphylococcus aureus, which may be associated with mutations in the tst promoter.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Kohei Ogura, Hiroka Furuya, Natsuki Takahashi, Kana Shibata, Maho Endo, Shinya Watanabe, Longzhu Cui, Tohru Miyoshi-Akiyama, Shigefumi Okamoto, Kazuhiro Ogai, Junko Sugama
Summary: This study analyzed the characteristics of Staphylococcus spp. on healed skin before recurrence and found that the imbalance between two strains of Staphylococcus leads to recurrent pressure injuries.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Respiratory System
Yasumaro Fujiki, Naoko Mato, Shinya Watanabe, Tomoki Shibano, Ken Tonai, Kento Takahashi, Tatsuya Saito, Akiko Okuyama, Ayako Takigami, Masashi Bando, Takuji Suzuki, Longzhu Cui, Koichi Hagiwara
Summary: This study presented a case of life-threatening pneumonia caused by P. aeruginosa in a healthy 67-year-old man. Rapid disseminated infection led to hemorrhagic pneumonia and bacteremia. Radical pneumonectomy eventually resolved the infection.
RESPIRATORY MEDICINE CASE REPORTS
(2022)
Article
Multidisciplinary Sciences
Jumpei Fujiki, Tomohiro Nakamura, Keisuke Nakamura, Keita Nishida, Yurika Amano, Yusaku Watanabe, Satoshi Gondaira, Masaru Usui, Masaru Shimizu, Kazuhiko Miyanaga, Shinya Watanabe, Tomohito Iwasaki, Kotaro Kiga, Tomoko Hanawa, Hidetoshi Higuchi, Teiji Sawa, Yasunori Tanji, Yutaka Tamura, Longzhu Cui, Hidetomo Iwano
Summary: The study assessed the biological stability and clinical potential of Staphylococcus virus Phi SA012. The virus showed a wide host range and efficient lytic activity. Experiments on mice demonstrated the ability of Phi SA012 to spread and accumulate in specific organs without causing inflammation. Additionally, the study highlighted the importance of appropriate dosing and administration periods for effective phage therapy.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Hiroka Furuya, Kohei Ogura, Norihiko Takemoto, Shinya Watanabe, Ayaka Yamazaki, Kazuhiro Ogai, Junko Sugama, Shigefumi Okamoto
Summary: In this study, we developed a multiplex-nested PCR method to determine S. aureus MLST in samples collected from human skins. Using this assay, we succeeded in typing sequence types (STs) of S. aureus in the DNA samples derived from the skins healed from pressure injury. Therefore, this culture-free approach may be used in detecting S. aureus in clinical specimens without isolation.
MICROBIOLOGY AND IMMUNOLOGY
(2023)
Letter
Allergy
Okuto Iwasawa, Koji Kamiya, Takayuki Suzuki, Shinya Watanabe, Longzhu Cui, Daiki Karube, Soichiro Kado, Takeo Maekawa, Mayumi Komine, Mamitaro Ohtsuki
Summary: This article reports a rare case of cutaneous M. chelonae infection requiring differential diagnosis of M. stephanolepidis infection.
JOURNAL OF CUTANEOUS IMMUNOLOGY AND ALLERGY
(2023)