Article
Microbiology
You Zhang, Juan Carlos de la Torre, Gregory B. Melikyan
Summary: This study investigates the role of human LAMP1 in LASV fusion and reveals that ectopic expression of hLAMP1 accelerates the kinetics of small fusion pore formation but only modestly increases LASV fusion and infection. Furthermore, it is found that hLAMP1 is involved in the dilation of LASV fusion pores in the late stage of fusion. These findings shed light on the previously unappreciated role of hLAMP1 in promoting dilation of LASV fusion pores.
Article
Biochemistry & Molecular Biology
Leonardo Cassari, Angela Pavan, Giulia Zoia, Monica Chinellato, Elena Zeni, Alessandro Grinzato, Sylvia Rothenberger, Laura Cendron, Monica Dettin, Antonella Pasquato
Summary: The SARS-CoV-2 virus displays unique characteristics and has a high ability to spread among humans. The virus relies on Furin for its invasion and replication, and though there are mutations in the amino acid sequence, the dependence on Furin remains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Michael Katz, Jonathan Weinstein, Maayan Eilon-Ashkenazy, Katrin Gehring, Hadas Cohen-Dvashi, Nadav Elad, Sarel J. Fleishman, Ron Diskin
Summary: Lassa virus, a human pathogen, has a spike complex on its surface that facilitates cell entry. The structure of the spike complex reveals the role of the signal peptide in stabilizing the complex and provides insights into the mechanism of binding with the host cell.
Article
Virology
Alba Torrents de la Pena, Ivan del Moral Sanchez, Judith A. Burger, Ilja Bontjer, Gozde Isik, Dirk Eggink, Marit J. van Gils, Rogier W. Sanders
Summary: Directed virus evolution was used to enhance the stability of the HIV-1 envelope glycoprotein (Env) trimer. By deliberately destabilizing the interface between Env subunits, compensatory mutations were identified in specific Env regions, indicating a potential to strengthen intersubunit interactions and enhance trimer stability. This study sheds light on the structural mechanisms involved in Env trimer stability, which can inform future immunogen design.
JOURNAL OF VIROLOGY
(2021)
Review
Microbiology
Joshua J. Tu, Victoria Maksimova, Lee Ratner, Amanda R. Panfil
Summary: HTLV-1 is an oncogenic human retrovirus that causes lifelong infection. It can lead to aggressive malignancies or progressive neurodegenerative diseases. Treatment options for HTLV-1-associated diseases are limited and the prognosis for ATL is poor. Early acquisition of the virus increases the risk of disease development. Currently, there is no cure or reliable prevention measure for HTLV-1 infection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Xiaoqian Xin, Yifeng Liu, Lei Guo, Hui Wang, Daiqiang Lu, Yaotian Chang, Mingming Wan, Yong Zhang, Yaming Shan, Qiao Zhang, Xiaowen Liu, Feng Gao
Summary: The HIV virus poses a major threat to human health with over 84 million people infected. Developing a vaccine has been challenging due to the high genetic variation of the virus. However, a new HIV-1 envelope glycoprotein nanoparticle vaccine has been designed using amphiphilic polymers, which shows potent and broad neutralizing activities against multiple HIV-1 subtypes. Additionally, this vaccine remains stable under different storage conditions and can potentially be applied to other protein-based vaccines.
Article
Environmental Sciences
Anna Bizon, Grzegorz Franik, Pawel Madej
Summary: This study found significantly higher PCSK9 concentration and lower high-density lipoprotein concentration in the serum of women with PCOS compared to those without, as well as significant differences in paraoxonase 1 activities. Higher PCSK9 concentration in women with PCOS, especially those with insulin resistance, may be associated with hormonal concentrations. The study suggests that changes in paraoxonase 1 activities and lipid profile parameters in women with PCOS could be linked to metabolism disorders, but further research is needed due to the small sample size.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Hyun Ju Jung, Suk Won Choi, Kyung-Hwan Boo, Jee-Eun Kim, Young Kyoung Oh, Min Kyun Han, Moon Young Ryu, Chang Woo Lee, Christian Moller, Pratik Shah, Gu Min Kim, Woorim Yang, Seok Keun Cho, Seong Wook Yang
Summary: The protein HCS1 is identified as a cytoplasmic protease that degrades HYL1, which is important for miRNA biogenesis. The activity of HCS1 is regulated by light and darkness, with COP1 suppressing its activity in light and promoting its activity in darkness.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Asif Ali, Tingkai Wu, Hongyu Zhang, Peizhou Xu, Syed Adeel Zafar, Yongxiang Liao, Xiaoqiong Chen, Hao Zhou, Yutong Liu, Wenming Wang, Xianjun Wu
Summary: This study reports the novel role of SUBSrP1 in anther cuticle biosynthesis through ROS-mediated programmed cell death in rice. The anthers of apa1331 from the apical spikelets showed degeneration, pollen-less, and defects in cuticle formation. The high expression of OsSUBSrP1 in the upper portion of the panicle led to phenotypic defects in apical spikelets.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Maura Mobilia, Callie Whitus, Alexander Karakashian, Hong S. Lu, Alan Daugherty, Scott M. Gordon
Summary: This study highlighted the significant impact of Dennd5b on plasma and hepatic lipid concentrations, as well as its role in resistance to PCSK9-induced hypercholesterolemia in mice lacking Dennd5b. The findings also revealed smaller atherosclerotic lesions and reduced hepatic lipid content in Dennd5b(-/-) mice, associated with differential expression of genes involved in hepatic lipid metabolism.
JOURNAL OF LIPID RESEARCH
(2022)
Article
Virology
Aurelie Drouin, Julie Migraine, Marie-Alice Durand, Alain Moreau, Julien Burlaud-Gaillard, Maxime Beretta, Philippe Roingeard, Melanie Bouvin-Pley, Martine Braibant
Summary: The cellular factor IFITM3 reduces HIV-1 infectivity through an unclear mechanism. IFITM3 interacts with Env of sensitive viruses, inducing conformational changes that decrease viral infectivity, but this antiviral action is modulated by the nature of Env, particularly the V1V2 and V3 loops, which may escape this interaction after processing in the Golgi apparatus.
JOURNAL OF VIROLOGY
(2021)
Article
Multidisciplinary Sciences
Jeremie Prevost, Yaozong Chen, Fei Zhou, William D. Tolbert, Romain Gasser, Halima Medjahed, Manon Nayrac, Dung N. Nguyen, Suneetha Gottumukkala, Ann J. Hessell, Venigalla B. Rao, Edwin Pozharski, Rick K. Huang, Doreen Matthies, Andres Finzi, Marzena Pazgier
Summary: Resistance to the HIV-1 entry inhibitor temsavir is not solely determined by residue 375, but also involves other residues within the gp120 inner domain layers. The resistance is mediated by crosstalk between residue 375 and the inner domain layers. Additionally, temsavir has the ability to adjust its binding mode to accommodate changes in Env conformation, contributing to its broad antiviral activity.
NATURE COMMUNICATIONS
(2023)
Review
Virology
Tianshu Xiao, Yongfei Cai, Bing Chen
Summary: HIV-1 infection begins with virion attachment to host cell membrane through Env, binding to receptors CD4 and coreceptors CCR5/CXCR4; understanding the structural biology of HIV-1 Env aids in designing antiviral therapeutics targeting viral entry.
Article
Virology
Nicholas Weaver, Jason Hammonds, Lingmei Ding, Grigoriy Lerner, Krista Dienger-Stambaugh, Paul Spearman
Summary: The molecular motor protein KIF16B regulates the outward sorting step involved in human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env) trafficking, limiting lysosomal degradation and enhancing particle incorporation. The absence of KIF16B reduces Env expression on cell surface, leading to a decrease in Env incorporation into particles and particle infectivity. HIV-1 replication is substantially reduced in KIF16B knockout cells.
JOURNAL OF VIROLOGY
(2023)
Article
Microbiology
Yuta Hikichi, Rachel Van Duyne, Phuong Pham, Jennifer L. Groebner, Ann Wiegand, John W. Mellors, Mary F. Kearney, Eric O. Freed
Summary: The presence of mutations in the HIV-1 envelope glycoprotein (Env) can lead to reduced susceptibility to multiple classes of antiretroviral drugs (ARVs) and may contribute to virological failure in patients. The accumulation of Env mutations in individuals failing integrase inhibitor therapy suggests a possible mechanism for drug resistance development without mutations in target genes. Understanding how Env mutations affect drug resistance may influence therapeutic strategies for ARV-treated patients.
Article
Biotechnology & Applied Microbiology
Abdelmalik Khalafalla, Yan Li, Anna Uehara, Nasareldien A. Hussein, Jing Zhang, Ying Tao, Eric Bergeron, Ibrahim H. Ibrahim, Mohamed A. Al Hosani, Mohd F. Yusof, Zulaikha M. Alhammadi, Saeed M. Alyammahi, Esmat F. Gasim, Hassan Z. A. Ishag, Farida A. L. Hosani, Susan Gerber, Salama S. Almuhairi, Suxiang Tong
Summary: Crimean-Congo haemorrhagic fever virus (CCHFV) is a tick-borne virus that causes a deadly disease known as Crimean-Congo haemorrhagic fever (CCHF) with a high fatality rate. Evidence of CCHFV has been found in dromedary camels in the UAE, with a novel lineage identified in camels and potential reassortment of the genome's M segment.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Oncology
Tra Ly Nguyen, Marie-Julie Nokin, Silvia Teres, Mercedes Tome, Clement Bodineau, Oriane Galmar, Jean-Max Pasquet, Benoit Rousseau, Sebastian van Liempd, Juan Manuel Falcon-Perez, Elodie Richard, Elodie Muzotte, Hamid-Reza Rezvani, Muriel Priault, Marion Bouchecareilh, Isabelle Redonnet-Vernhet, Julien Calvo, Benjamin Uzan, Francoise Pflumio, Patricia Fuentes, Maria L. Toribio, Abdel-Majid Khatib, Pierre Soubeyran, Piedad Del Socorro Murdoch, Raul Duran
Summary: Upregulation of Notch1 in T-ALL induces metabolic changes in glutamine, leading to increased glutamine addiction. Notch1 also activates the mTORC1 pathway through increased glutaminolysis, promoting cell growth. Combined treatment targeting mTORC1 and limiting glutamine availability has a synergistic effect in inducing apoptosis and preventing Notch1-driven leukemia progression.
MOLECULAR ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Daniela Gerovska, Patricia Garcia-Gallastegi, Jean Descarpentrie, Olatz Crende, Maria Casado-Andres, Ander Martin, Jokin Eguia, Abdel-Majid Khatib, Marcos J. Arauzo-Bravo, Iker Badiola
Summary: Colon cancer remains a major health issue globally, and evidence suggests cancer stem cells may play a crucial role in its development, with proprotein convertases possibly playing a role in regulating malignant phenotypes. Targeting proprotein convertases could be a new potential therapeutic strategy for colon cancer, given their impact on cell proliferation, differentiation, and chemoresistance.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Bruno O. Villoutreix, Vincent Calvez, Anne-Genevieve Marcelin, Abdel-Majid Khatib
Summary: The SARS-CoV-2 virus exploits ACE2 as a receptor for cell invasion, with certain mutations potentially enhancing its transmission capabilities and pathogenicity through effects on ACE2 binding. The UK strain may exhibit higher affinity for ACE2, while the transmission level of the South African strain could be influenced by the N501Y substitution.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Isabel Galeano-Otero, Raquel Del Toro, Abdel-Majid Khatib, Juan Antonio Rosado, Antonio Ordonez-Fernandez, Tarik Smani
Summary: Angiogenesis is a complex multistep process regulated by factors like VEGF and changes in Ca2+ concentration. Studies have shown that SARAF and Orai1 play crucial roles in angiogenesis, and their disruption can impact HUVEC tube formation and migration. These findings provide new insights into the mechanisms of angiogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Asheley P. Chapman, Xiaoling Tang, Joo R. Lee, Asiya Chida, Kristina Mercer, Rebekah E. Wharton, Markus Kainulainen, Jennifer L. Harcourt, Roosecelis B. Martines, Michelle Schroeder, Liangjun Zhao, Anton Bryksin, Bin Zhou, Eric Bergeron, Brigid C. Bollweg, Azaibi Tamin, Natalie Thornburg, David E. Wentworth, David Petway, Dennis Bagarozzi, M. G. Finn, Jason M. Goldstein
Summary: This study reports the development and functional characterization of 29 nM-affinity mouse SARS-CoV-2 mAbs created by an accelerated immunization and hybridoma screening process, highlighting their differing functions such as binding diverse protein epitopes, viral neutralization, impact on RBD-hACE2 binding, and immunohistochemical staining of infected lung tissue, which were correlated with variable gene usage and sequence.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Jose J. Lopez, Geraldine Siegfried, Carlos Cantonero, Fabienne Soulet, Jean Descarpentrie, Tarik Smani, Iker Badiola, Simon Pernot, Serge Evrard, Juan A. Rosado, Abdel-Majid Khatib
Summary: Furin, a proprotein convertase involved in the maturation of various proproteins, plays a role in tumorigenesis and tumor progression. Inhibition of Furin can enhance calcium influx in triple negative breast cancer cells, leading to reduced malignant phenotype and increased sensitivity to apoptosis inducers. This suggests that targeting Furin with its inhibitor ppFurin may be a potential strategy for interfering with Ca2+ mobilization and reducing breast cancer cell resistance to treatments.
Article
Pharmacology & Pharmacy
Sergio E. Rodriguez, David W. Hawman, Teresa E. Sorvillo, T. Justin O'Neal, Brian H. Bird, Luis L. Rodriguez, Eric Bergeron, Stuart T. Nichol, Joel M. Montgomery, Christina F. Spiropoulou, Jessica R. Spengler
Summary: This article reviews the progress in immunobiology research of Crimean-Congo hemorrhagic fever virus (CCHFV), including the relationship between different outcomes and disease severity, and provides recommendations for future research.
ANTIVIRAL RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Zongsheng He, Abdel-Majid Khatib, John W. M. Creemers
Summary: Furin, as the first discovered proprotein convertase member, plays diverse roles in cancer by regulating the maturation of various protein precursors. Understanding the functions and regulations of Furin in cancer cells is crucial for developing effective strategies in cancer treatment.
Review
Immunology
Simon Pernot, Serge Evrard, Abdel-Majid Khatib
Summary: This article discusses the importance of the reciprocal interactions between tumor cells and stromal cells in the evolution and survival of cancer cells, as well as the impact of immune cells and other cells on tumor progression.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Jean Descarpentrie, Marcos J. Arauzo-Bravo, Zongsheng He, Alexia Francois, Alvaro Gonzalez, Patricia Garcia-Gallastegi, Iker Badiola, Serge Evrard, Simon Pernot, John W. M. Creemers, Abdel-Majid Khatib
Summary: This study reveals that the malignant phenotype of colorectal cancer stem cells can be repressed by inhibition of the proprotein convertase Furin, leading to reduced expression of stem cell markers LGR5 and Nanog, as well as dysregulated expression of several calcium regulators. Targeting Furin may provide a potential therapeutic approach for colorectal cancer stem cells.
Review
Oncology
Alexia Francois, Jean Descarpentrie, Iker Badiola, Geraldine Siegfried, Serge Evrard, Simon Pernot, Abdel-Majid Khatib
Summary: Immunotherapy is an advanced clinical management for various cancers, achieved by rebuilding aberrant immune surveillance on cancers through in vivo or ex vivo engineering of efficient immune cells. The programming of immune cells using different strategies has improved their therapeutic influence and specificity. However, effective immunotherapy must consider the complete complexity of immune cell function.
BRITISH JOURNAL OF CANCER
(2023)
Article
Cell Biology
Malak Alannan, Hala Fatrouni, Veronique Trezeguet, Franziska Dittrich-Domergue, Patrick Moreau, Geraldine Siegfried, Benjamin Liet, Abdel-Majid Khatib, Christophe F. Grosset, Bassam Badran, Hussein Fayyad-Kazan, Aksam J. Merched
Summary: The inhibition of PCSK9 in liver cancer cells can effectively suppress cell proliferation and enhance vulnerability to iron-triggered lipid peroxidation. Targeting the key metabolic player PCSK9 may be a potential therapeutic approach for treating liver cancers.
Article
Cell Biology
Celine Gounou, Flora Bouvet, Benjamin Liet, Valerie Prouzet-Mauleon, Lena d'Agata, Etienne Harte, Francoise Argoul, Geraldine Siegfried, Richard Iggo, Abdel-Majid Khatib, Anthony Bouter
Summary: ANXA5 and ANXA6 are crucial components of the membrane repair machinery in MDA-MB-231 cells and are correlated with the invasiveness of breast cancer cells. High expression of ANXA5 and ANXA6 predicts poor prognosis in high-grade lung, gastric, and breast cancers. Genetic inhibition of ANXA5 and ANXA6 in zebrafish significantly reduces tumor cell dissemination.
BIOLOGY OF THE CELL
(2023)
Review
Biochemistry & Molecular Biology
Marta Martin-Bornez, Debora Falcon, Rosario Morrugares, Geraldine Siegfried, Abdel-Majid Khatib, Juan A. A. Rosado, Isabel Galeano-Otero, Tarik Smani
Summary: Myocardial infarction (MI) leads to the loss of cardiac myocytes and damage to the coronary microcirculation, which exceeds the regenerative capacity of the heart. The repair process after MI involves angiogenesis, fibroblast proliferation, and scar formation. Various signaling pathways, including Notch, Wnt, PI3K, and modulation of intracellular Ca2+ concentration, regulate angiogenesis in the infarcted heart. Additionally, cell-to-cell communication through extracellular vesicles containing pro-angiogenic proteins and miRNAs plays a role in cardiac repair after MI.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)