Article
Genetics & Heredity
Zuzanna Cyske, Lidia Gaffke, Karolina Pierzynowska, Grzegorz Wegrzyn
Summary: Monogenic diseases are caused by mutations in a single gene, and they have been considered as genetic disorders with the simplest mechanisms. However, recent studies have revealed that the molecular mechanisms of monogenic diseases can be unexpectedly complicated, requiring complex studies at the molecular level. In the case of mucopolysaccharidoses (MPS), a group of monogenic lysosomal storage diseases, the expression of hundreds of genes is significantly altered, indicating a more complex mechanism than previously thought.
Article
Biochemistry & Molecular Biology
Sara E. Lewis, Courtney B. Rosencrance, Evan De Vallance, Andrew Giromini, Xena M. Williams, Joo-Yeun Oh, Heidi Schmidt, Adam C. Straub, Paul D. Chantler, Rakesh P. Patel, Eric E. Kelley
Summary: Research indicates that XOR does not contribute to nitrite reduction in human and rodent erythrocytes, suggesting a need to validate immuno-detectable XOR by demonstrating enzymatic activity.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Veterinary Sciences
Kang Zhang, Jingyan Zhang, Lei Wang, Qiang Liang, Yuhui Niu, Linlin Gu, Yanming Wei, Jianxi Li
Summary: This study used transcriptomics and proteomics techniques to analyze differentially expressed proteins and genes in cow peripheral blood mononuclear cells (PBMCs) after BVDV-1 infection. The results showed that BVDV-1 primarily suppresses the host through specific pathways, while PBMCs counteract viral invasion through different mechanisms. This study is important for understanding the mechanism of BVDV-1 infection and host responses.
VETERINARY SCIENCES
(2023)
Article
Nutrition & Dietetics
Fernando Canovas, Maria Salud Abellan-Ruiz, Ana Maria Garcia-Munoz, Antonio Jesus Luque-Rubia, Desiree Victoria-Montesinos, Silvia Perez-Pinero, Maravilla Sanchez-Macarro, Francisco Javier Lopez-Roman
Summary: This study demonstrates the efficacy of eggshell membrane supplement in relieving joint pain and stiffness, improving the quality of life for patients with knee joint pain. The dosage of the supplement has a dose-dependent response on the outcomes.
Article
Multidisciplinary Sciences
Xingyu Chen, Dongning Chen, Ehsan Ban, Kimani C. Toussaint, Paul A. Janmey, Rebecca G. Wells, Vivek B. Shenoy
Summary: Cells can sense and respond to mechanical forces in fibrous extracellular matrices, and this phenomenon, known as long-range force transmission, is influenced by the presence of glycosaminoglycans (GAGs) in the matrix. GAGs increase the stiffness of the collagen network and hinder the alignment of collagen fibers, thus impeding cellular force transmission over long distances.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Engineering, Biomedical
Zeina Habli, Nataly Naser Al Deen, Waddah Malaeb, Nadine Mahfouz, Angela Mermerian, Rabih Talhouk, Rami Mhanna
Summary: Alginate sulfates (AlgSulf) show a robust inhibitory effect on breast cancer cells in cell culture models, selectively targeting cancer cells while leaving normal cells unaffected. These findings suggest that AlgSulf may represent a promising new class of anticancer biomimetic glycosaminoglycans.
ACTA BIOMATERIALIA
(2021)
Article
Oncology
Moussa Ndiaye, Gora Diop, Celine Derbois, Jean-Louis Spadoni, Josselin Noirel, Raissa Medina-Santos, Cedric Coulonges, Magali Torres, Alioune Dieye, Mbacke Sembene, Jean-Francois Deleuze, Alain Toledano, Ahmadou Dem, Jean-Francois Zagury, Sigrid Le Clerc
Summary: This study performed a transcriptomic analysis of PBMCs from Senegalese women with CC and identified differential gene expression patterns. The IL-1 pathway and downregulation of the T-cell receptor alpha gene were found to be involved in CC. These findings have potential implications for the development of clinical biomarkers for CC prevention and should be validated in other populations.
Article
Multidisciplinary Sciences
Xiao Liu, Ivy Aneas, Noboru Sakabe, Rebecca L. Anderson, Christine Billstrand, Cristina Paz, Harjot Kaur, Brian Furner, Seong Choi, Adriana Y. Prichina, Elizabeth Ann L. Enninga, Haidong Dong, Amy Murtha, Gregory E. Crawford, John A. Kessler, William Grobman, Marcelo A. Nobrega, Sarosh Rana, Carole Ober
Summary: The mechanisms underlying the timing of labor in humans are largely unknown. This study aimed to characterize the cells at the maternal-fetal interface (MFI) in term and preterm pregnancies in Black women. High-dimensional single-cell resolution analysis revealed distinct cell populations at the MFI, including immune and non-immune cells. Perturbations in the PD1/PD-L1 pathway at the MFI may play a role in the onset of spontaneous preterm labor.
SCIENTIFIC REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
Hengwei Yu, Sayed Haidar Abbas Raza, Yueting Pan, Gong Cheng, Chugang Mei, Linsen Zan
Summary: This study used blood transcriptome sequencing and metabolite detection to explore the differential genes and metabolites associated with the subcutaneous fat depot phenotype in beef cattle. The functional genes SMPD3 and CERS1, as well as the metabolite sphingosine 1-phosphate, were identified as important factors contributing to the differences in phenotype. These findings provide important reference value for effective breeding to improve beef performance.
Article
Multidisciplinary Sciences
Kevin M. Magnaye, Selene M. Clay, Jessie Nicodemus-Johnson, Katherine A. Naughton, Janel Huffman, Matthew C. Altman, Daniel J. Jackson, James E. Gern, Douglas K. Hogarth, Edward T. Naureckas, Steven R. White, Carole Ober
Summary: Maternal asthma is a consistent risk factor for asthma in children, potentially impacting diverse epigenetically mediated pathways leading to distinct subtypes of severe asthma in adults, including hard-to-treat T2-low asthma. Analysis shows differences in DNA methylation patterns between mothers with and without asthma in genes related to impaired T cell signaling and response to pathogens. These findings may have implications for understanding and treating different subtypes of asthma.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Immunology
Lucy-Jayne Marsh, Samuel Kemble, Patricia Reis Nisa, Ruchir Singh, Adam P. Croft
Summary: Rheumatoid arthritis is an immune-mediated inflammatory disease where fibroblasts play a significant role, both in maintaining joint homeostasis and promoting disease progression during inflammation. Recent studies have revealed the heterogeneity of fibroblasts, challenging the previous notion of their homogeneity and providing new insights into their role in inflammatory joint pathology.
IMMUNOLOGICAL REVIEWS
(2021)
Article
Biotechnology & Applied Microbiology
Kyu-Sang Lim, Jian Cheng, Austin Putz, Qian Dong, Xuechun Bai, Hamid Beiki, Christopher K. Tuggle, Michael K. Dyck, Pig Gen Canada, Frederic Fortin, John C. S. Harding, Graham S. Plastow, Jack C. M. Dekkers
Summary: Investigation of gene expression profiles in blood of young healthy pigs revealed associations with disease resilience, growth rate, health scores, mortality, and feed conversion rate. Genes involved in stress response, heme metabolism, and viral gene expression were found to be associated with host response to disease.
Article
Plant Sciences
Jiping Xiao, Xiaoyu Xu, Maoxing Li, Xiaojie Wu, Huachun Guo
Summary: This study investigated the molecular mechanisms of anthocyanin biosynthesis in purple sweet potatoes by combining transcriptomic and metabolomic analysis. The significantly enhanced expression of multiple structural genes was found to be responsible for the higher accumulation of anthocyanin in purple sweet potatoes. Additionally, the competition or redistribution of intermediate substrates played a crucial role in the coloration of purple and non-purple materials. Moreover, chlorogenic acid production seemed to be an interrelated but independent pathway differentiated from anthocyanin biosynthesis.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Infectious Diseases
Mengyao Li, Mohammad A. K. Azad, Philip E. Thompson, Kade D. Roberts, Tony Velkov, Yan Zhu, Jian Li
Summary: Polymyxins are last-line antibiotics used to treat Gram-negative 'superbugs'. However, they can cause nephrotoxicity in up to 60% of patients. This study investigated the transcriptomic responses of human renal tubular cells to polymyxins and identified potential targets for designing safer new-generation polymyxins.
Article
Ophthalmology
James T. Rosenbaum, Christina A. Harrington, Robert P. Searles, Suzanne S. Fei, Amr Zaki, Sruthi Arepalli, Michael A. Paley, Lynn M. Hassman, Albert T. Vitale, Christopher D. Conrady, Puthyda Keath, Claire Mitchell, Lindsey Watson, Stephen R. Planck, Tammy M. Martin, Dongseok Choi
Summary: This study aimed to categorize idiopathic uveitis into subtypes based on gene expression from blood. Gene expression profiling achieved an overall accuracy of 85% in differentiating uveitis associated with diagnosable systemic diseases. Results suggest that peripheral blood gene expression profiling could be a valuable tool in aiding differential diagnosis of uveitis causes.
AMERICAN JOURNAL OF OPHTHALMOLOGY
(2021)
Article
Nutrition & Dietetics
Catalina Amadora Pomar, Catalina Pico, Andreu Palou, Juana Sanchez
Summary: This study finds that the diet of nursing mothers can affect lipid metabolism in their offspring and attenuate their ability to burn calories from an obesogenic diet in adulthood.
Article
Nutrition & Dietetics
Alba Serrano, Andreu Palou, M. Luisa Bonet, Joan Ribot
Summary: This study found that mild NR supplementation during early postnatal life has beneficial effects on lipid and energy metabolism in skeletal muscle and liver in adulthood, with potential preventive strategies against obesity-related disorders.
Article
Biochemistry & Molecular Biology
Catalina A. Pomar, Pedro Castillo, Andreu Palou, Mariona Palou, Catalina Pico
Summary: This study aimed to evaluate the impact of improving maternal nutrition during lactation on the levels of specific miRNA in the mammary gland and milk of diet-induced obese rats. The results showed that implementing a healthy diet during lactation can normalize the expression levels of specific miRNA and some target genes in the mammary gland of obese dams but not in milk.
Article
Biochemistry & Molecular Biology
Marta Alonso-Bernaldez, Antoni Asensio, Andreu Palou-March, Juana Sanchez, Andreu Palou, Francisca Serra, Mariona Palou
Summary: This study investigated the effects of maternal diet during lactation on the expression of leptin, adiponectin, and related miRNAs in breast milk, as well as their impact on dams and their offspring. The results showed that a diet enriched with oleic acid, betaine, and leucine during lactation can affect the ratio of leptin to adiponectin in breast milk, leading to increased body fat in offspring and higher insulin resistance in adulthood. Additionally, specific miRNAs in breast milk were also influenced by the maternal diet.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Pere Bibiloni, Catalina A. Pomar, Andreu Palou, Juana Sanchez, Francisca Serra
Summary: Elevated miR-222 levels are linked to metabolic syndrome, insulin resistance, and diabetes. Rats fed an obesogenic diet during lactation have higher miR-222 content in breast milk, leading to increased body fat mass and impaired insulin sensitivity in their offspring. Transfection experiments with miR-222 mimics or inhibitors on 3T3-L1 preadipocytes reveal that miR-222 plays a role in pathways related to insulin signaling, lipid metabolism, and adipogenesis.
Article
Agriculture, Multidisciplinary
Francisco Garcia-Carrizo, Sebastia Galmes, Catalina Pico, Andreu Palou, Ana Maria Rodriguez
Summary: Supplementation with high-esterified pectin (HEP) can reverse metabolic malprogramming associated with gestational undernutrition. HEP supplementation improves blood pressure, reduces heart lipid content, and regulates gene expression in the heart. Furthermore, HEP supplementation increases circulating levels of fibroblast growth factor 21.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Catalina A. Pomar, M. Luisa Bonet, Adrian Ferre-Beltran, Pablo A. Fraile-Ribot, Mercedes Garcia-Gasalla, Melchor Riera, Catalina Pico, Andreu Palou
Summary: The study reveals that gene expression patterns in blood cells from SARS-CoV-2 infected individuals are associated with clinical phenotypes and body mass index (BMI) in COVID-19 patients. mRNA levels of several genes were found to be increased in the blood cells of COVID-19 patients compared to controls, and some genes were also associated with disease severity and obesity status.
Article
Nutrition & Dietetics
Pedro Castillo, Catalina Amadora Pomar, Andreu Palou, Mariona Palou, Catalina Pico
Summary: The study aimed to analyze the long-term metabolic effects of leptin supplementation during suckling in the offspring of diet-induced obese rats and the potential benefits of improving maternal diet during lactation. The results showed that leptin treatment significantly improved metabolic profile and body fat accumulation under standard diet conditions, with stronger effects in male offspring. However, under high-fat diet conditions, the offspring of dams fed as high-fat diet showed metabolic alterations that were not observed in the offspring of dams switched to standard diet during lactation. Leptin supplementation improved glucose homeostasis in male offspring but impaired insulin sensitivity in female offspring born to high-fat diet-fed dams. These findings highlight the importance of balanced maternal diet during the perinatal period, especially lactation, for the metabolic health of offspring, and the beneficial effects of leptin supplementation during suckling, particularly in male offspring.
Article
Biochemistry & Molecular Biology
Pedro Castillo, Ondrej Kuda, Jan Kopecky, Catalina Amadora Pomar, Andreu Palou, Mariona Palou, Catalina Pico
Summary: The study evaluated the impact of improving maternal diet during lactation on the metabolome of milk and offspring plasma in diet-induced obese rats. The results showed that the milk of rats fed a western diet had altered amino acid and carnitine profiles, as well as changes in other metabolites. These alterations were also observed in the plasma of the offspring, but were largely normalized in rats that switched to a standard diet during lactation.
Article
Nutrition & Dietetics
Marta Alonso-Bernaldez, Andreu Palou-March, Rocio Zamanillo-Campos, Andreu Palou, Mariona Palou, Francisca Serra
Summary: Although nutrient profiling systems are helpful for healthier food choices, assessing overall diet quality is still necessary. This study developed a diet profiling algorithm (DPA) to evaluate nutritional quality, giving a final score and color coding. The DPA considers various factors such as carbohydrate/fiber ratio and energy from saturated fats and sodium, as well as fiber, protein, and macronutrient distribution.
Article
Multidisciplinary Sciences
Sebastia Galmes, Azahara I. I. Ruperez, Juana Sanchez, Luis A. A. Moreno, Ronja Foraita, Antje Hebestreit, Denes Molnar, Andreu Palou, Catalina Pico
Summary: Insufficient PA in children is a major contributor to obesity and cardiometabolic complications. This study aimed to identify transcript-based biomarkers for early discernment of low PA in children. The analysis revealed differentially expressed genes related to cardiometabolic benefits and metabolic complications, as well as affected pathways associated with protein catabolism, skeletal morphogenesis, and wound healing.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Sebastia Galmes, Andreu Palou, Francisca Serra
Summary: The study found that suboptimal vitamin A intake is associated with cardiometabolic risk, with differences observed between sexes and genetic backgrounds. The findings highlight the importance of personalized nutritional advice regarding vitamin A and cardiometabolic risk.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pediatrics
Catalina Pico, Empar Lurbe, Jaap Keijer, Jan Kopecky, Jean-Francois Landrier, Julio Alvarez-Pitti, Jean Charles Martin, Paula Oliver, Andreu Palou, Mariona Palou, Petr Zouhar, Joan Ribot, Ana M. Rodriguez, Juana Sanchez, Francisca Serra, M. Luisa Bonet
Summary: The objective of the INTEGRActiv study is to identify novel integrative biomarkers of PA and its effects on body health in children and adolescents, and to validate these biomarkers through an educational intervention.
FRONTIERS IN PEDIATRICS
(2023)
