Article
Medicine, Research & Experimental
Defu Chen, David W. Nauen, Hyeon-Cheol Park, Dawei Li, Wu Yuan, Ang Li, Honghua Guan, Carmen Kut, Kaisorn L. Chaichana, Chetan Bettegowda, Alfredo Quinones-Hinojosa, Xingde Li
Summary: This study systematically investigated the feasibility of using multiphoton microscopy for rapid histopathological assessment of label-and processing-free surgical specimens, focusing on infiltrating glioma. The results showed that multiphoton microscopy robustly captures diagnostic features of glioma and deep learning can assist with image recognition.
Article
Medicine, Research & Experimental
Xingxin Huang, Yuan-E Lian, Lida Qiu, XunBin Yu, Jikui Miao, Shichao Zhang, Zheng Zhang, Xiong Zhang, Jianxin Chen, Yannan Bai, Lianhuang Li
Summary: Nonalcoholic fatty liver disease is a common cause of chronic liver disease worldwide. This study used multiphoton microscopy to visualize important pathological features of nonalcoholic fatty liver disease and developed image analysis algorithms for quantifying disease progression. These findings can contribute to a better understanding of the disease and potential therapeutic targets.
LABORATORY INVESTIGATION
(2023)
Article
Multidisciplinary Sciences
Chenxi Qian, Kun Miao, Li-En Lin, Xinhong Chen, Jiajun Du, Lu Wei
Summary: VISTA is a label-free superresolution method combining sample expansion and vibrational imaging, enabling high-resolution interrogation of protein-rich biological structures in cells and tissues, with a resolution down to 78 nm.
NATURE COMMUNICATIONS
(2021)
Review
Optics
Natan T. Shaked, Stephen A. Boppart, Lihong V. Wang, Juergen Popp
Summary: This Review provides a comprehensive overview and comparison of different label-free imaging modalities, aiming to facilitate collaboration, technological advancements, and biophysical discoveries, as well as new avenues in clinical detection, diagnosis, and monitoring of diseases.
Article
Gastroenterology & Hepatology
Lucas Kreiss, Oana-Maria Thoma, Sarah Lemire, Kristina Lechner, Birgitta Carle, Ashwathama Dilipkumar, Timo Kunert, Kristina Scheibe, Christina Heichler, Anna-Lena Merten, Benno Weigmann, Clemens Neufert, Kai Hildner, Michael Vieth, Markus F. Neurath, Oliver Friedrich, Sebastian Schurmann, Maximilian J. Waldner
Summary: This study demonstrates the potential of label-free imaging for in vivo evaluation of inflammation in inflammatory bowel diseases. The authors developed a multiphoton colitis score (MCS) based on label-free imaging analysis, and validated its accuracy using traditional hematoxylin and eosin scoring. The results show that the MCS can effectively discriminate between healthy and inflamed tissue in various colitis models, and specific characteristics of each phenotype were identified. This quantitative imaging method holds promise for monitoring inflammatory pathways in murine models and could aid in the development of new therapeutic approaches for inflammatory bowel diseases.
INFLAMMATORY BOWEL DISEASES
(2022)
Article
Optics
D. Septier, V Mytskaniuk, R. Habert, D. Labat, K. Baudelle, A. Cassez, G. Brevalle-Wasilewski, M. Conforti, G. Bouwmans, H. Rigneault, A. Kudlinski
Summary: We present a highly multimodal nonlinear micro-endoscope with a diameter of 2 mm, which enables label-free imaging of biological tissues. The micro-endoscope utilizes double-clad antiresonant hollow core fiber and graded-index fiber technology, allowing for various imaging modes and achieving high-resolution images.
Article
Biochemical Research Methods
Xingxin Huang, Yuan-E Lian, Lida Qiu, XunBin Yu, Zhenlin Zhan, Zheng Zhang, Xiong Zhang, Hongxin Lin, Shuoyu Xu, Jianxin Chen, Yannan Bai, Lianhuang Li
Summary: In this study, multiphoton microscopy was used for label-free imaging of liver tissues to directly detect various components including collagen fibers, tumors, blood vessels, and lymphocytes. A deep learning classification model was developed to automatically identify tumor regions with an accuracy of 0.998. An automated image processing method was introduced to extract eight collagen morphological features from different stages of liver diseases. Statistical analysis showed significant differences between them, indicating the potential use of these quantitative features in monitoring fibrotic changes during the progression of liver diseases. Therefore, multiphoton imaging combined with automatic image processing holds promising future for rapid and label-free diagnosis of liver diseases.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Multidisciplinary Sciences
Alberto Aguilar, Alain Abou Khalil, David Pallares Aldeiturriaga, Xxx Sedao, Cyril Mauclair, Pierre Bon
Summary: Ultrafast laser processing can induce surface nanostructurating in materials with dimensions close to the laser wavelength. The characterization of these nanostructures is crucial for fine-tuning laser parameters and generating complex or hybrid nanostructures. However, the characterization of Laser Induced Periodic Surface Structures (LIPSS) in a non-destructive manner is challenging due to their small dimensions. This paper demonstrates the use of a modified label-free Confocal Reflectance Microscope (CRM) in a photon reassignment regime to detect sub-diffraction limit LIPSS, showing the metrological efficiency compared to commercial SEM.
SCIENTIFIC REPORTS
(2023)
Review
Engineering, Biomedical
Irene Georgakoudi, Kyle P. Quinn
Summary: In the past fifty years, the autofluorescence of NADH and FAD has been studied in various cell types and diseases. With the advancement of nonlinear optical microscopy in biomedical research, NADH and FAD imaging provides a noninvasive method to monitor cell and tissue status and understand dynamic changes in metabolism. Different tools and methods have been developed to measure the properties of NADH and FAD autofluorescence, but further work is needed to fully understand metabolism. This article discusses the current understanding of our optical sensitivity to metabolic pathways and the challenges in the field.
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING
(2023)
Article
Multidisciplinary Sciences
Boram Lee, Eunsoo Lee, June Hoan Kim, Hyung-Jin Kim, Yong Guk Kang, Hyun Jung Kim, Jin-Kyoung Shim, Seok-Gu Kang, Beop-Min Kim, Karam Kim, Youseok Kim, Keunchang Cho, Woong Sun
Summary: The study introduces a method for label-free imaging of brain slices by enhancing regional heterogeneity of optical properties using tissue clearing principle. By retaining more lipids in the white matter and adjusting clearing conditions to enhance refractive index differences in different regions, the method can be used for micro-scale monitoring studies.
Article
Oncology
Bohan Zhang, Tengteng Yao, Yaxin Chen, Chuqiao Wang, Yongyang Bao, Zhaoyang Wang, Keke Zhao, Minbiao Ji
Summary: Femtosecond time-resolved pump-probe microscopy can provide label-free and chemical-specific detection of melanin species in uveal melanoma, and delineate the interface between melanoma and paracancerous regions on various tissue conditions. Transcriptome sequencing confirms the presence of active eumelanin synthesis in uveal melanoma.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Takahiro Matsui, Akio Iwasa, Masafumi Mimura, Seiji Taniguchi, Takao Sudo, Yutaka Uchida, Junichi Kikuta, Hidetomo Morizono, Rie Horii, Yuichi Motoyama, Eiichi Morii, Shinji Ohno, Yasujiro Kiyota, Masaru Ishii
Summary: This study proposes an imaging-based histopathological examination method using multiphoton excitation microscopy to visualize unfixed/unstained human breast tissues. It demonstrates the potential of label-free MPM imaging for real-time automatic diagnosis of breast cancer.
Review
Biochemistry & Molecular Biology
Giulia Borile, Deborah Sandrin, Andrea Filippi, Kurt I. Anderson, Filippo Romanato
Summary: Multiphoton microscopy has been actively used in biomedical research for the past 30 years, offering label-free imaging and a range of applications. This technique, including two-photon autofluorescence and harmonic generation, provides versatile and detailed sample analysis for various research fields. The future of label-free multiphoton microscopy holds promise for further advancements in the study of biological samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Electrical & Electronic
Lingxiao Yang, Jaena Park, Marina Marjanovic, Eric J. Chaney, Darold R. Spillman, Heidi Phillips, Stephen A. Boppart
Summary: Intraoperative imaging in surgical oncology can provide valuable information about the tumor microenvironment and margin by visualizing microstructural features and molecular dynamics. Most current techniques use exogenous labels, which may disrupt the natural environment and alter the optical signatures. Portable nonlinear imaging systems have enabled real-time detection and diagnosis of tissue during surgery, showing potential for assisting in cancer diagnosis.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2021)
Article
Biochemical Research Methods
Chunlei Jiang, Hangyu Yue, Bing Yan, Taiji Dong, Xiangyu Cui, Peng Chen, Zengbo Wang
Summary: The study demonstrated the use of yeast cells as imaging lenses, trapping and magnifying nanoscale objects using fiber-based optical trapping technique. Experimental results showed that yeast cells could clearly resolve 100 nm features on a Blu-ray disc.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Biochemistry & Molecular Biology
Yang Guo, Anna-Lena Merten, Ulrike Schoeler, Ze-Yan Yu, Jasmina Cvetkovska, Diane Fatkin, Michael P. Feneley, Boris Martinac, Oliver Friedrich
Summary: Cardiac mechano-electric coupling is a complex feedback mechanism that regulates cardiac function through Ca2+ signals in acute and chronic pressure overload scenarios. While the molecular mechanisms of how mechanical forces alter cardiac function remain unclear, mechanosensitive channels are believed to play a significant role in cardiac mechano-electric coupling.
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
(2021)
Article
Engineering, Biomedical
Abbas Shafiee, Amanda S. Cavalcanti, Navid T. Saidy, Dominik Schneidereit, Oliver Friedrich, Akhilandeshwari Ravichandran, Elena M. De-Juan-Pardo, Dietmar W. Hutmacher
Summary: The biomimetically designed mPCL dressings, combined with human cell seeding, promote wound healing, reduce scar formation, and enhance skin regeneration.
Article
Biochemistry & Molecular Biology
Tom Zimmermann, Lisa A. Gebhardt, Lucas Kreiss, Christin Schneider, Stephanie Arndt, Sigrid Karrer, Oliver Friedrich, Michael J. M. Fischer, Anja-Katrin Bosserhoff
Summary: CAP is a partially ionized gas with reported antitumor effects, but its active components and molecular mechanisms are not fully understood. This study characterized the impact of acidified nitrite on melanoma cells, demonstrating a synergistic effect with acidification leading to strong cytotoxicity. It also highlighted the importance of RNS during CAP treatment and evaluated important molecular mechanisms involved in the cancer cell response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Sarah Lemire, Oana-Maria Thoma, Lucas Kreiss, Simon Voelkl, Oliver Friedrich, Markus F. Neurath, Sebastian Schuermann, Maximilian J. Waldner
Summary: The activity of immune cells is a major factor in the progression of inflammatory bowel diseases. This study aims to understand the autofluorescence changes depending on the type and activation state of immune cells. The results show that innate immune cells have significantly increased autofluorescence signals compared to adaptive immune cells. In vitro stimulation also increases autofluorescence signals in adaptive immune cells and macrophages. Cell death leads to a significant decrease in NADH autofluorescence, while FAD signals remain unaffected. These findings demonstrate the value of autofluorescence as a tool to characterize immune cells in different functional states.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Nina Simon, Cornelia Voigtlaender, Barbara Kappes, Petra Rohrbach, Oliver Friedrich
Summary: Drug resistance often arises from mutations in solute transporters, which may result in the ability of transporters to actively transport drugs away from their target sites. This study examined the transport characteristics of different strains of Plasmodium falciparum with various pfmdr1 gene mutations. The results showed that the drug-resistant strain Dd2 had the highest transport rates, followed by K1 and the drug-sensitive strain 3D7, which correlated with their gene copy numbers. This assay provides a means to evaluate the likelihood of resistance formation for newly developed drugs in the early stages of drug development.
Article
Chemistry, Multidisciplinary
Matthias Ryma, Hatice Genc, Ali Nadernezhad, Ilona Paulus, Dominik Schneidereit, Oliver Friedrich, Kristina Andelovic, Stefan Lyer, Christoph Alexiou, Iwona Cicha, Juergen Groll
Summary: This study presents a facile and flexible approach for integrating biomimetically branched microvasculature within bulk hydrogels. By creating sacrificial scaffolds using melt electrowriting and placing them in a bioreactor filled with hydrogel precursor solution, physiological-like branchings and channels can be generated. This approach allows for rapid formation of a tight and biologically functional endothelial layer.
ADVANCED MATERIALS
(2022)
Article
Biochemical Research Methods
Lucas Kreiss, Ingo Ganzleben, Alexander Muehlberg, Paul Ritter, Dominik Schneidereit, Christoph Becker, Markus F. Neurath, Oliver Friedrich, Sebastian Schuermann, Maximilian Waldner
Summary: This study integrated Raman spectroscopy and multiphoton microscopy to investigate the biochemical composition and morphological features related to cellular components and connective tissue. The results showed significant increase in collagen levels in the fibrosis model and high correlation between autofluorescence signatures assigned to immune cells and disease severity. The study also examined the impact of integrated data sets on prediction performance in machine learning models.
JOURNAL OF BIOPHOTONICS
(2022)
Article
Oncology
Philipp Kunze, Lucas Kreiss, Vendula Novosadova, Adriana V. Roehe, Sara Steinmann, Jan Prochazka, Carol I. Geppert, Arndt Hartmann, Sebastian Schuermann, Oliver Friedrich, Regine Schneider-Stock
Summary: In this study, the ECM remodeling potential of colorectal tumor cells was investigated using multiphoton microscopy and the chorioallantoic membrane assay. The results showed that DAPK1 ko tumor cells have a high ECM remodeling potential at the tissue level.
Article
Biochemistry & Molecular Biology
Kai Peter Sommer, Adrian Krolinski, Mohammad Mirkhalaf, Hala Zreiqat, Oliver Friedrich, Martin Vielreicher
Summary: The goal of bone tissue engineering is to build artificial bone tissue that closely resembles human bone and supports its integration into the body. This study used two types of 3D-printed scaffolds to seed and differentiate osteoblastic cells, and tested different assays and imaging methods to monitor these processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Mena Michael, Larisa Kovbasyuk, Paul Ritter, Michael B. B. Reid, Oliver Friedrich, Michael Haug
Summary: An oxidizing redox state has unique effects on muscle contraction. However, our understanding of the elasticity and flexibility of muscle fibers is limited. The MyoRobot platform allows us to investigate the calcium responsiveness of muscle contraction and examine the passive stress-strain behavior. Our findings show that an oxidizing environment leads to decreased force generation and increased passive stress in muscle fibers.
Article
Multidisciplinary Sciences
Charlotte Gineste, Sonia Youhanna, Sabine U. Vorrink, Sara Henriksson, Andres Hernandez, Arthur J. Cheng, Thomas Chaillou, Andreas Buttgereit, Dominik Schneidereit, Oliver Friedrich, Kjell Hultenby, Joseph D. Bruton, Niklas Ivarsson, Linda Sandblad, Volker M. Lauschke, Hakan Westerblad
Summary: This study investigated the effects of extracellular matrix (ECM) disruption on cellular and mitochondrial morphology, gene expression, and Ca2+ handling in skeletal muscle fibers. The results showed that enzymatic dissociation of fibers resulted in myopathic phenotypes, shorter and less branched mitochondria, and prolonged mitochondrial Ca2+ accumulation during repeated contractions. However, this accumulation was concealed with enzymatic dissociation, making it an ambiguous method in studies of native intracellular Ca2+ fluxes.
Article
Biochemistry & Molecular Biology
Anna Rederer, Victoria Rose, Rene Krueger, Linda Schmittutz, Izabela Swierzy, Lena Fischer, Ingo Thievessen, Julian Bauer, Oliver Friedrich, Mario Schiffer, Janina Mueller-Deile
Summary: Signaling-pathway analyses and investigation of gene responses are usually done in 2D monocultures, but glomerular cells grow in 3D and have different interactions. We cultured glomerular cells in both 2D and 3D monocultures and co-cultures to study cell behavior, gene expression, and cell-cell interactions. Our results showed that 3D co-cultures exhibited increased expression of cell-specific markers and extracellular matrix compared to 2D co-cultures. Additionally, using 3D co-cultures confirmed intercellular communication between podocytes and glomerular endothelial cells. These findings question the reliability of 2D monocultures and suggest that glomerular 3D co-cultures are more suitable for studying cell communication, disease modeling, and drug screening.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Biomaterials
T. Distler, L. Kretzschmar, D. Schneidereit, S. Girardo, R. Goswami, O. Friedrich, R. Detsch, J. Guck, A. R. Boccaccini, S. Budday
Summary: This study investigates the properties of hydrogels containing cells and microgel beads through complex mechanical analyses. The results show that the stiffness of hydrogels significantly decreases when the cell and bead concentrations exceed certain values. Hydrogels with higher cell concentrations exhibit more pronounced material nonlinearity for larger strains and faster stress relaxation.
BIOMATERIALS SCIENCE
(2021)
Article
Medicine, Research & Experimental
Dominik Schneidereit, Anita Broellochs, Paul Ritter, Lucas Kreiss, Zeinab Mokhtari, Andreas Beilhack, Gerhard Kroenke, Jochen A. Ackermann, Maria Faas, Anika Grueneboom, Sebastian Schuermann, Oliver Friedrich
Summary: Structural remodeling or damage in tissues is often unevenly distributed depending on the location and extent of damaging stimuli. By optimizing different clearing protocols, aqueous solution-based protocols using 2,2'-thiodiethanol (TDE) were found to be advantageous in preserving muscle morphology. Applying TDE clearing allows for the assessment of muscle fiber integrity in 3D and quantification of muscle necrosis.
