Article
Immunology
Caiqin Wang, Qing He, Yingxuan Yin, Yinjuan Wu, Xuerong Li
Summary: This study established a cell line with stable overexpression of CsGRN in normal hepatocytes, and found that CsGRN promoted cell proliferation by regulating the expression of cell-cycle-related genes, as well as regulated the malignant metastasis of liver cells possibly through the EGFR-mediated signaling pathways.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Yaqiong Yang, Yu Sun, Rong Hu, Jia Yan, Ziheng Wang, Wenlong Li, Hong Jiang
Summary: This study investigated the regulatory mechanism of epidermal growth factor receptor (EGFR) on microglial activation induced by morphine in BV-2 cells. The results showed that morphine treatment increased CD11b expression, EGFR phosphorylation, ERK1/2 activation, cell migration, and production of proinflammatory cytokines, which were inhibited by blocking the EGFR signaling pathway with AG1478. These findings suggest that the EGFR/ERK signaling pathway could be a novel pharmacological strategy to attenuate morphine tolerance by suppressing microglial activation.
Article
Biochemistry & Molecular Biology
Zhenzhen Pan, Kai Wang, Xiniao Wang, Zhirong Jia, Yuqi Yang, Yalei Duan, Lianzhan Huang, Zhuo-Xun Wu, Jian-ye Zhang, Xuansheng Ding
Summary: This study reveals a common molecular mechanism underlying EGFR-TKIs resistance in non-small cell lung cancer, involving cholesterol accumulation, EGFR/Src/Erk/SP1 axis-mediated ERR alpha re-expression and subsequent cell proliferation. Lowering cholesterol levels and downregulating ERR alpha can overcome resistance to gefitinib and osimertinib.
Article
Cell Biology
Eyleen Corrales, Ella Levit-Zerdoun, Patrick Metzger, Ralf Mertes, Ariane Lehmann, Julia Munch, Steffen Lemke, Silke Kowar, Melanie Boerries
Summary: This study comprehensively characterized a panel of patient-derived BRAFV600E positive melanoma cell lines and found that a decrease in sensitivity to MAPK/ERK pathway inhibition corresponded with increased migratory potential and the acquisition of stem cell-like properties. Additionally, it was discovered that increased activity of the PI3K/AKT pathway lead to a decreased dependency on MAPK/ERK signaling in melanoma cells.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Ya Han, Hengmin Wang
Summary: This study explored the role and mechanism of miR-3918 in glioma malignancy. The findings showed that miR-3918 overexpression impaired the proliferative and migratory capacities of glioma cells by inactivating PI3K/AKT and ERK pathways. Additionally, it was confirmed that miR-3918 targeted EGFR and EGFR overexpression can restore the inactivated PI3K/AKT and ERK pathways caused by miR-3918 and influence glioma cell proliferation and migration.
JOURNAL OF MOLECULAR NEUROSCIENCE
(2022)
Article
Cell Biology
Shanshan Duan, Loredana Moro, Rui Qu, Daniele Simoneschi, Hyunwoo Cho, Shaowen Jiang, Huiyong Zhao, Qing Chang, Elisa de Stanchina, Arnaldo A. Arbini, Michele Pagano
Summary: FBXO31 acts as a tumor suppressor by promoting the degradation of DUSP6 and inhibiting ERK signaling while activating PI3K-AKT signaling. Deletion of FBXO31 enhances tumor development in prostate cancer models, but treatment with a small molecule inhibitor of DUSP6 can prevent tumor formation by suppressing AKT activation. These findings highlight the importance of the FBXO31-DUSP6 axis in regulating signaling pathways and its potential therapeutic implications in prostate cancer.
Article
Genetics & Heredity
Brady G. Strittmatter, Travis J. Jerde, Peter C. Hollenhorst
Summary: ERG is a common oncogene in prostate cancer that can interact with AKT protein to regulate gene expression and promote luminal epithelial differentiation. Mutant ERG can drive prostate tumorigenesis without AKT activation, and ERG can promote tumorigenesis independent of cell differentiation.
Article
Multidisciplinary Sciences
Xiao-Ping Zhao, Xiao-Li Zheng, Min Huang, Ya-Jia Xie, Xiao-Wen Nie, Ali Adnan Nasim, Xiao-Jun Yao, Xing-Xing Fan
Summary: Despite advances in clinical and diagnostic techniques, the treatment of non-small cell lung cancer (NSCLC) remains unsatisfactory in terms of cure and survival rates. Epidermal growth factor (EGFR) is recognized as a key driver of NSCLC and a target for pharmacological intervention. DMU-212, an analog of resveratrol, has shown inhibitory effects on various types of cancer, but its effect on lung cancer is unclear. This study aimed to investigate the effects and underlying mechanism of DMU-212 on EGFR-mutant NSCLC cells. The results showed that DMU-212 had higher cytotoxicity on EGFR-mutant NSCLC cell lines compared to normal lung epithelial cells. Further analysis revealed that DMU-212 induced G2/M phase arrest in H1975 and PC9 cells by regulating cell cycle-related proteins and modulating the expression of EGFR and key signaling molecules. In conclusion, this study suggests that DMU-212 inhibits NSCLC growth by targeting AMPK and EGFR.
Review
Pharmacology & Pharmacy
Jieun Bang, Mihyeon Jun, Soyun Lee, Hyuk Moon, Simon Weonsang Ro
Summary: Hepatocellular carcinoma (HCC) is a significant global health concern, with its incidence steadily increasing. Recent progress has been made in understanding the molecular signaling pathways, particularly the EGFR/PI3K/AKT/mTOR pathway, which plays a central role in HCC. Preclinical studies have shown the effectiveness of targeting this signaling pathway for HCC therapy, offering potential therapeutic options for patients.
Article
Cell Biology
Jinhuan Wu, Yuping Chen, Rui Li, Yaping Guan, Mu Chen, Hui Yin, Xiaoning Yang, Mingpeng Jin, Bingsong Huang, Xin Ding, Jie Yang, Zhe Wang, Yiming He, Qianwen Wang, Jian Luo, Ping Wang, Zhiyong Mao, Michael S. Y. Huen, Zhenkun Lou, Jian Yuan, Fanghua Gong
Summary: The study reveals that CDK2 regulates the ERK pathway through USP37, promoting cancer cell proliferation. Additionally, the combination of CDK1/2 and EGFR inhibitors shows a synergistic anticancer effect by reducing the stability and activity of ERK1/2.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Mihoko Kato, Irina Kolotuev, Alexandre Cunha, Shahla Gharib, Paul W. Sternberg
Summary: This study reveals the function of muscarinic receptor GAR-3 in epithelial cell migration in C. elegans, including receiving signals from cholinergic neurons, affecting migratory path, and determining migration direction. The experimental results suggest that the regulation of GAR-3 receptor in LC is accomplished by specific downstream signaling pathways, and there is a clear correlation between GAR-3 receptor activation level and LC migration direction.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Oncology
Jian Sun, Lin Chen, Ming Dong
Summary: The study revealed that miR-338-5p controls epithelial-mesenchymal transition and metastasis of pancreatic cancer cells by targeting the EGFR/ERK pathway, and its expression in clinical analysis is negatively associated with lymph node metastasis and AJCC staging.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Wei Wang, Xinhang Xia, Kuifei Chen, Meng Chen, Yinnan Meng, Dongqing Lv, Haihua Yang
Summary: PHLPP, a potential tumor suppressor, is found to be a key regulator of sensitivity to EGFR-TKIs in NSCLC. Loss of PHLPP function leads to activation of survival pathways, reducing sensitivity to EGFR-TKIs. Enhancing PHLPP expression may be a valuable strategy for delaying or overcoming EGFR-TKIs drug resistance.
FRONTIERS IN ONCOLOGY
(2021)
Article
Pharmacology & Pharmacy
Nuray Uremis, Yusuf Turkoz, Muhammed Mehdi Uremis, Yilmaz Cigremis, Emine Salva
Summary: In this study, the anticarcinogenic effects of sorafenib-cucurbitacin D and sorafenib-cucurbitacin I combination on HepG2 cell line were investigated. The results showed that the combination of cucurbitacin D and I with sorafenib had a synergistic effect, inhibiting cell proliferation, migration, and angiogenesis.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Jing Huang, Zhi-Feng Xu, Feng Liu, An-Ni Song, Hua Su, Chun Zhang
Summary: MCM6 plays an important role in renal fibrosis, with overexpression promoting fibrosis recovery and depletion exacerbating fibrosis development. Decreased expression of DUSP6 may be involved in MCM6-induced renal fibrosis.
