Article
Cell Biology
Meaghan E. Beckner, Lauren Thompson, Patrick N. Radcliffe, Rebecca Cherian, Marques Wilson, Nicholas Barringer, Lee M. Margolis, J. Philip Karl
Summary: This study aimed to identify sex differences in changes in serum metabolome related to body composition, physical performance, and endocrine and metabolic status during a 17-day military training exercise. The results showed that women preferentially mobilize fat stores compared to men during sustained military training, which may help mitigate the loss of lean mass and lower body power.
PHYSIOLOGICAL GENOMICS
(2023)
Article
Chemistry, Applied
Xiao Chen, Zixuan Gu, Yinghan Peng, Siew Young Quek
Summary: Camembert cheese undergoes biochemical changes during ripening, with lipolysis and proteolysis being the primary events. Flavor compounds were also found to contribute to the unique aroma. The ripening process was characterized by an increase in proteolysis and the development of odorants. Specific volatile markers were identified to differentiate the ripening stages of Camembert cheese.
Article
Chemistry, Applied
Andrea Asensio-Grau, Joaquim Calvo-Lerma, Ana Heredia, Ana Andres
Summary: The study found that processing techniques and intestinal conditions have different effects on the digestion of salmon. Protein digestion was most affected by processing, while intestinal conditions were the major determinant of fat digestion.
Article
Food Science & Technology
Shuwen Zhang, Tong Wang, Yumeng Zhang, Bo Song, Xiaoyang Pang, Jiaping Lv
Summary: The use of Monascus as an adjunct starter in the production of Camembert-type cheese was found to enhance proteolysis, lipolysis, and volatile compounds, resulting in improved flavor and taste.
Article
Endocrinology & Metabolism
Ming Gao, Minjian Chen, Qiuzhen Chen, Shuai Zhu, Hengjie Wang, Weizheng Yang, Xi Wang, Qiang Wang, Ling Gu
Summary: Well-controlled metabolism is crucial for optimal oocyte development. This study investigated the intrinsic regulation of oocyte maturation through metabolomics and transcriptomics analysis, revealing novel metabolic features during porcine oocyte meiosis. The findings provide a comprehensive data resource and potential molecular biomarkers for predicting and improving oocyte quality.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Roberto Stella, Raphael Severino Bonadio, Stefano Cagnin, Maria Lina Massimino, Alessandro Bertoli, Caterina Peggion
Summary: This study investigated the proteomic profile and secreted metabolome of ALS astrocytes derived from transgenic mouse models, revealing depleted proteins and secreted metabolites involved in glutathione metabolism and signaling, as well as altered transcription factor activation and lipid metabolism in ALS astrocytes expressing mutated hSOD1. These findings provide insights into potential therapeutic targets to address astrocyte dysfunctionalities and altered astrocyte-MN crosstalk in ALS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Physiology
Yingjiang Xu, Huan Liu, Dianfeng Han, Lihua Ren, Xianghong Gong, Fang Jiang, Yanmei Cui, Xiaojing Liu, Chuanbo Ren, Jinglin Xue, Xiuhui Tian
Summary: The study revealed that the metabolic changes in the digestive system of mantis shrimp under Cd stress, with 91 metabolites differentially expressed mainly in amino acids, phospholipids, and fatty acid esters. The functional pathways of differential metabolites showed significant effects on biological processes such as amino acid metabolism, transmembrane transport, energy metabolism, and signal transduction. The Cd-induced oxidative stress and energy metabolism disorders were characterized by the differential expression of amino acids and ADP in mantis shrimp, while the interference of transmembrane transport and signal transduction was due to the differential expression of phospholipids.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Food Science & Technology
Oceance Savary, Emmanuel Coton, Marie-Bernadette Maillard, Frederic Gaucheron, Christophe Le Meur, Jens Frisvad, Anne Thierry, Jean-Luc Jany, Monika Coton
Summary: This study aimed to evaluate the functional impact of Bisifusarium domesticum and other cheese-associated fungi on cheese-making. Their lipolytic and proteolytic activities, as well as their potential to produce volatile and non-volatile secondary metabolites, were assessed. Four novel cheese-associated species, including Bisifusarium allantoides, Bisifusarium penicilloides, Longinectria lagenoides, and Long-inectria verticilliformis, were described, and these species showed potential in lipid production.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Nutrition & Dietetics
Longwei Xi, Gang Zhai, Yulong Liu, Yulong Gong, Qisheng Lu, Zhimin Zhang, Haokun Liu, Junyan Jin, Xiaoming Zhu, Zhan Yin, Shouqi Xie, Dong Han
Summary: This study blocked glucose uptake by knocking out glut2 in zebrafish and found that impaired insulin signaling-mediated anabolism and enhanced AMPK signaling-mediated catabolism contribute to energy homeostasis remodeling caused by blocked glucose uptake. These findings reveal the mechanism of energy homeostasis remodeling and provide a potential strategy for adapting to low glucose levels.
FRONTIERS IN NUTRITION
(2023)
Article
Food Science & Technology
James A. Elegbeleye, Elna M. Buys
Summary: This study aimed to quantify and compare biofilm-induced proteolysis and lipolysis in Bacillus subtilis and Bacillus velezensis with planktonic cells. Differences were observed in cell counts and enzyme concentrations in biofilms and planktonic cells in UHT milk. The spoilage potential of biofilms and planktonic cultures was found to be strain-dependent with variations in proteolysis and lipolysis among different strains.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Aurelie Thooft, Raphael Conotte, Jean-Marie Colet, Karim Zouaoui Boudjeltia, Patrick Biston, Michael Piagnerelli
Summary: Inflammatory processes in ICU patients can lead to metabolic changes and increased risks of morbidity and mortality. This study aimed to determine if metabolomics can aid in prognosis at ICU admission. Metabolic profiles of ICU patients were compared to healthy volunteers, and differences were found in septic shock patients. However, these metabolite profiles were not correlated with mortality. Changes in metabolic products were observed in septic shock patients on the first day of ICU admission, but these changes did not predict prognosis.
Article
Biochemistry & Molecular Biology
Dongqin Wei, Qian Sun, Yizhou Li, Chaowei Li, Xinjian Li, Chao Sun
Summary: The study revealed that leptin can reduce the m(6)A methylation of Plin5 by promoting FTO, affecting lipid metabolism and energy consumption, providing a theoretical basis for treating diseases related to obesity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Si-Lan Han, Yan Liu, Samwel M. Limbu, Li-Qiao Chen, Mei-Ling Zhang, Zhen-Yu Du
Summary: In this study, Atglistatin, an ATGL-specific inhibitor, was added to the zebrafish diet and led to severe fat deposition, reduced oxygen consumption, oxidative stress, and inflammation. The Atglistatin-treated fish showed elevated HSL protein expression but reduced free fatty acids and lipase activities, as well as decreased autophagy marker. Glycogenolysis was stimulated while blood glucose was higher in the Atglistatin-treated fish, indicating a loss of lipid-sourced energy production due to ATGL inhibition.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Environmental Sciences
Ce Shi, Yangfang Ye, Feng Pei, Changkao Mu, Chunlin Wang
Summary: The study found that using a transport bag containing oxygen and seawater can effectively extend the post-capture survival time of small swimming crabs, possibly due to metabolic adaptation including a switch in energy generation pathway and decline in certain metabolic processes. Energy homeostasis is crucial for crab survival during transport.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Food Science & Technology
Mariam Sardinas-Valdes, Hugo Sergio Garcia-Galindo, Alfonso Juventino Chay-Canul, Jose Rodolfo Velazquez-Martinez, Josafat Alberto Hernandez-Becerra, Angelica Alejandra Ochoa-Flores
Summary: The addition of nano-emulsified curcumin to hair sheep milk resulted in cheeses with increased fat and ash levels, as well as higher total phenolic content and antioxidant activity during ripening. Lipolysis increased steadily in the first 40 days of ripening, while proteolysis increased consistently throughout the ripening process. This study highlights the potential of using nano-emulsified curcumin to enhance the functionality of dairy foods.
Letter
Hematology
Micaela Kalani Roy, Rebecca Burgoyne Wilkerson, Keisha Alexander, Natalie J. Nokoff, Melanie Cree-Green, Angelo D'Alessandro
Article
Critical Care Medicine
Margot DeBot, Christopher Erickson, Marguerite Kelher, Terry R. Schaid, Ernest E. Moore, Angela Sauaia, Alexis Cralley, Ian LaCroix, Angelo D'Alessandro, Kirk Hansen, Mitchell J. Cohen, Christopher C. Silliman, Julia Coleman
Summary: By studying the sex differences in platelets and cryoprecipitate coagulation factors, it was found that blood products from female donors are more effective in improving coagulation dysfunction compared to male blood products. Additionally, female-donor cryoprecipitate contains more factor V and factor XIII, and several proteins with potential immunological significance. These sex differences may influence how transfusions modulate thromboinflammation caused by trauma, suggesting the potential role of sex-specific transfusion guidelines in hemostatic resuscitation.
JOURNAL OF TRAUMA AND ACUTE CARE SURGERY
(2023)
Article
Critical Care Medicine
Terry R. Schaid, Ian LaCroix, Kirk C. Hansen, Angelo D'Alessandro, Ernest E. Moore, Angela Sauaia, Monika Dzieciatkowska, Margot DeBot, Alexis L. Cralley, Otto Thielen, William Hallas, Christopher Erickson, Sanchayita Mitra, Anirban Banerjee, Kenneth Jones, Christopher C. Silliman, Mitchell J. Cohen
Summary: This study identified the proteomic signature of trauma-induced NETosis and revealed that NETosis may mediate organ dysfunction following severe injury. SerpinB1 was identified as a major component of this NET protein profile and could serve as an early marker of excessive NETosis after injury.
JOURNAL OF TRAUMA AND ACUTE CARE SURGERY
(2023)
Article
Critical Care Medicine
Terry R. Schaid Jr, Mitchell J. Cohen, Angelo D'Alessandro, Christopher C. Silliman, Ernest E. Moore, Angela Sauaia, Monika Dzieciatkowska, William Hallas, Otto Thielen, Margot DeBot, Alexis Cralley, Ian LaCroix, Christopher Erickson, Sanchayita Mitra, Anirban Banerjee, Kenneth Jones, Kirk C. Hansen
Summary: Severe injury can lead to organ dysfunction, and hemolysis of red blood cells may contribute to this process. Hemolysis can release erythrocyte proteins, disrupting arginine metabolism and reducing NO production. This study aimed to quantify hemolysis and arginine metabolism in trauma patients and measure their association with injury severity, transfusions, and outcomes.
Article
Biochemistry & Molecular Biology
Ji Young Lee, Reece P. Stevens, Viktoriya V. Pastukh, Viktor M. Pastukh, Natalya Kozhukhar, Mikhail F. Alexeyev, Julie A. Reisz, David Nerguizian, Angelo D'Alessandro, Anna Koloteva, Meredith S. Gwin, Justin T. Roberts, Glen M. Borchert, Brant M. Wagener, Jean-Franc Comma Ois Pittet, Brian B. Graham, Kurt R. Stenmark, Troy Stevens
Summary: Pulmonary microvascular endothelial cells prefer glucose over fructose for glycolysis, and this study found that the enzyme PFKFB3 inhibits fructose metabolism in these cells. PFKFB3 knockout led to increased survival and enhanced fructose utilization in fructose-rich medium. The study also demonstrated that pneumonia is associated with increased fructose levels in the lung. These findings suggest that PFKFB3 is a key regulator of glucose versus fructose use in glycolysis in pulmonary microvascular endothelial cells.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2023)
Article
Oncology
Miles Piper, Maureen Hoen, Laurel B. Darragh, Michael W. Knitz, Diemmy Nguyen, Jacob Gadwa, Greta Durini, Idil Karakoc, Abby Grier, Brooke Neupert, Benjamin Van Court, Khalid N. M. Abdelazeem, Justin Yu, Nicholas A. Olimpo, Sophia Corbo, Richard Blake Ross, Tiffany T. Pham, Molishree Joshi, Ross M. Kedl, Anthony J. Saviola, Maria Amann, Pablo Umana, Laura Codarri Deak, Christian Klein, Angelo D'Alessandro, Sana D. Karam
Summary: In PDAC patients, the response to RT involves changes in IL-2Rb, IL-2Rg, and IL-2Ra expression. PD1-IL2v, a PD-1-targeted IL-2 variant immunocytokine, enhances tumor-specific T cell activation and reduces Treg suppression. In PDAC mouse models, PD1-IL2v treatment improves survival, increases tumor-infiltrating CD8+ T cells, and activates antigen-specific CD8+ T cells, especially when combined with single-dose RT.
Article
Biochemistry & Molecular Biology
Giulia Unali, Giovanni Crivicich, Isabel Pagani, Monah Abou-Alezz, Filippo Folchini, Erika Valeri, Vittoria Matafora, Julie A. Reisz, Anna Maria Sole Giordano, Ivan Cuccovillo, Giacomo M. Butta, Lorena Donnici, Angelo D'Alessandro, Raffaele De Francesco, Lara Manganaro, Davide Cittaro, Ivan Merelli, Carolina Petrillo, Angela Bachi, Elisa Vicenzi, Anna Kajaste-Rudnitski
Summary: The interferon-induced transmembrane proteins (IFITM) inhibit endocytic viral entry by binding to phosphatidylinositol 3,4,5-trisphosphate (PIP3), which plays a key role in endosomal antiviral immunity. The level of PIP3 is closely correlated with the potency of endosomal IFITM restriction, and exogenous PIP3 enhances the inhibition of endocytic viruses. These findings elucidate cell-compartment-specific antiviral mechanisms and have potential relevance for the development of broadly acting antiviral strategies.
Article
Biochemistry & Molecular Biology
Sachin Kumar, Jeffrey D. Vassallo, Kalpana J. Nattamai, Aishlin Hassan, Rebekah Karns, Angelika Vollmer, Karin Soller, Vadim Sakk, Mehmet Sacma, Travis Nemkov, Angelo D'Alessandro, Hartmut Geiger
Summary: It has been found that culturing hematopoietic stem cells (HSCs) at a pH of 6.9 for 2 days can maintain their potential, unlike cultivation at the commonly used pH of 7.4. These HSCs maintained at pH 6.9 exhibit smaller size, lower metabolic activity, weaker proliferation, and enhanced reconstitution ability upon transplantation compared to those cultivated at pH 7.4. This is due to the attenuation of the polyamine pathway in HSCs cultured at pH 6.9. Inhibiting the polyamine pathway in HSCs cultured at pH 7.4 with DFMO mimics the phenotypes and potential of HSCs cultured at pH 6.9. Exposing HSCs to a pH of 6.9 ex vivo positively regulates their function by reducing polyamines. These findings have the potential to improve short-term cultivation protocols for HSC transplantation and gene therapy interventions.
Article
Biochemical Research Methods
Sarah C. Skinner, Travis Nemkov, Mor Diaw, Maimouna Ndour Mbaye, Demba Diedhiou, Djiby Sow, Fatou Gueye, Philomene Lopez, Philippe Connes, Angelo D'Alessandro
Summary: Epidemiological data suggests that the prevalence of type 2 diabetes (T2D) will increase the most in sub-Saharan Africa over the next two decades. Metabolomics studies have identified biomarkers that could enhance T2D diagnosis and monitoring. However, there is a lack of research on the metabolome of individuals from sub-Saharan Africa.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Valentina Laghezza Masci, Davide Stefanoni, Angelo D'Alessandro, Marta Zambelli, Lorenzo Modesti, Daniele Pollini, Elisa Ovidi, Antonio Tiezzi
Summary: Cancer therapies often fail to completely eradicate cancer stem cells, leading to frequent relapses. This study investigated the mechanisms of resistance to the chemotherapeutic agent vinblastine in murine myeloma cells. The results suggest that altered amino acid uptake and metabolism may contribute to the acquisition of vinblastine resistance in blood cancer cells. These findings could be valuable for future research using human cell models.
Article
Physiology
Francesca I. Cendali, Travis Nemkov, Christina Lisk, Ian S. Lacroix, Seyed-Mehdi Nouraie, Yingze Zhang, Victor R. Gordeuk, Paul W. Buehler, David Irwin, Angelo D'Alessandro
Summary: This study investigated the exercise intolerance and metabolic changes in sickle cell disease patients using a mouse model and clinical cases. The results identified specific metabolites, including carboxylic acids, sphingosine 1-phosphate, and acylcarnitine, that were associated with reduced exercise tolerance in both mouse models and patients. These findings have important implications for understanding the mechanisms of exercise intolerance and developing preventive and therapeutic strategies for sickle cell disease.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Endocrinology & Metabolism
Shanlee M. Davis, Rhianna Urban, Angelo D'Alessandro, Julie A. Reisz, Christine L. Chan, Megan Kelsey, Susan Howell, Nicole Tartaglia, Philip Zeitler, Peter Baker Ii
Summary: Conditions related to cardiometabolic disease, including metabolic syndrome and type 2 diabetes, are common among men with Klinefelter syndrome (KS). The molecular mechanisms underlying this aberrant metabolism in KS are largely unknown. This study found that the plasma metabolome profile in adolescent males with KS is distinctly different from that in males without KS, suggesting differences in mitochondrial β-oxidation.
ENDOCRINE CONNECTIONS
(2023)
Article
Cell Biology
Eric. L. L. Bolf, Thomas. C. C. Beadnell, Madison. M. M. Rose, Angelo D'Alessandro, Travis Nemkov, Kirk. C. C. Hansen, Rebecca. E. E. Schweppe
Summary: Thyroid cancer is a common endocrine neoplasm with a poor prognosis for patients with metastatic disease or resistance to radioactive iodine. This study examines the effects of kinase inhibitors on thyroid cancer cells and reveals significant alterations in metabolite profiles, particularly in glycolysis, the TCA cycle, and amino acid levels. The study also highlights the accumulation of the tumor-suppressive metabolite 2-oxoglutarate and its potential in reducing thyroid cancer cell viability. These findings emphasize the importance of understanding how therapeutics reprogram metabolic processes and cancer cell behavior.
Article
Developmental Biology
Hiroko Kawakami, Katherine Q. Chen, Ruizhi Zhang, Matthew P. Pappas, Abigail Bailey, Julie A. Reisz, Dylan Corcoran, Ryuichi Nishinakamura, Angelo D'Alessandro, Yasuhiko Kawakami
Summary: Recent studies have shown that regulation of cellular metabolism, especially glycolysis and pathways branching from glycolysis, plays a crucial role in vertebrate embryo development. The transcription factor Sall4 is highly expressed in undifferentiated cells in developing mouse embryos and is involved in the regulation of glycolytic metabolism. Knockout experiments demonstrate that Sall4 restricts glycolysis in limb buds and is important for hindlimb patterning.
DEVELOPMENTAL BIOLOGY
(2023)
Article
Nutrition & Dietetics
Christopher Y. Kim, Hannah J. Larsen, Steven L. Spitalnik, Eldad A. Hod, Richard O. Francis, Krystalyn E. Hudson, Dominique E. Gordy, Elizabeth F. Stone, Sandy Peltier, Pascal Amireault, Angelo D'Alessandro, James C. Zimring, Paul W. Buehler, Xiaoyun Fu, Tiffany Thomas
Summary: High-dose fish oil intake reduces the quality of red blood cells, while low-dose fish oil improves the filterability of fresh red blood cells and reduces lipid peroxidation of stored red blood cells. However, low-dose fish oil does not improve the post-transfusion recovery capacity of stored red blood cells.