Article
Agronomy
Bentao Xiong, Linfang Wei, Yifan Wang, Jinyu Li, Xin Liu, Yunheng Zhou, Panpan Du, Hao Fang, Johannes Liesche, Yahong Wei, Jisheng Li, Shaolin Chen
Summary: Research on the model fungus Neurospora crassa reveals the important role of cellobiose in regulating the expression and secretion of lignocellulases, involving multiple levels such as proteome and phosphoproteome changes. It offers new insights and potential strategies for improving lignocellulase production.
GLOBAL CHANGE BIOLOGY BIOENERGY
(2021)
Article
Biochemistry & Molecular Biology
Mariana Villalba de la Pena, Pauliina A. M. Summanen, Neda N. Moghadam, Ilkka Kronholm
Summary: In nature, parental effects, where parents pass on information or resources to their offspring, are well documented in plants and animals. However, little is known about parental effects in fungi. In this study, we investigated the presence, duration, adaptive nature, and mechanism of parental effects in the filamentous fungus Neurospora crassa. We found a strong silver-spoon effect in initial mycelium growth, which lasted for one generation. The mechanism of this effect was determined to be spore glycogen content, glucose availability, and a radical transcription shift in spores.
Article
Microbiology
Nan-Nan Yu, Mayura Veerana, Wirinthip Ketya, Hu-Nan Sun, Gyungsoon Park
Summary: This study investigated the regulatory networks of nitric oxide (NO) in the filamentous fungus Neurospora crassa. Transcriptome analysis revealed the differential transcription of 424 genes after removing intracellular NO, which were mainly associated with carbohydrate and amino acid metabolism. Protein-protein interaction network analysis identified eight hub genes, including mss-4 and gel-3, that may play major roles during NO scavenging and influence vegetative growth. The study also found that NO scavenging inhibited the transcription of genes related to ribosome biogenesis.
Article
Microbiology
Ling Shen, Florence Chapeland-Leclerc, Gwenael Ruprich-Robert, Qiyi Chen, Siyu Chen, Muhammad Adnan, Jiangxin Wang, Gang Liu, Ning Xie
Summary: This study reveals the important role of the light desensitization protein VIVID (VVD) in regulating white light-responsive gene expression and secondary metabolite production in the filamentous fungus Podospora anserina. The loss of VVD function leads to exacerbated light responses and significant morphological and physiological changes.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nan-Nan Yu, Wirinthip Ketya, Gyungsoon Park
Summary: This study investigated the molecular signaling mechanism regulating cellulase production in Neurospora crassa. It was found that intracellular nitric oxide (NO) and cyclic AMP (cAMP) played a role in promoting transcription of cellulolytic enzymes and enhancing extracellular cellulolytic enzyme activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Nessma A. El-Zawawy, Sameh S. Ali, Maha A. Khalil, Jianzhong Sun, Hoda S. Nouh
Summary: Endophytic fungi produce bioactive compounds that have potential as drugs for treating various diseases. This study emphasizes the use of bioactive metabolites derived from endophytic fungi, particularly benzoic acid, to combat multidrug resistant pathogens in burn wounds. Benzoic acid was found to be safe and effective in treating the tested strains, including the highly resistant Staphylococcus aureus strain SA-17. Toxicological studies on animals showed that oral administration of benzoic acid at a high dose did not cause any toxicity. Histopathological examination confirmed that benzoic acid has promise as a safe and effective candidate for wound healing and combating MDR pathogens.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Genetics & Heredity
Midori Tabara, Hisashi Koiwa, Nobuhiro Suzuki, Toshiyuki Fukuhara
Summary: The study found that NcDCL2 is the sole siRNA producing Dicer in N. crassa, with enzymatic properties different from plant Dicers, as it can cleave both short and long dsRNAs.
FUNGAL GENETICS AND BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nan-Nan Yu, Wirinthip Ketya, Eun-Ha Choi, Gyungsoon Park
Summary: This study evaluated the potential of a non-thermal atmospheric pressure plasma jet to improve cellulolytic enzyme production efficiency. The results showed that plasma treatment significantly increased the activity and protein concentration of cellulolytic enzymes. Furthermore, plasma treatment promoted the transcription and secretion of cellulolytic enzymes by enhancing the intracellular levels of NO and Ca2+.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Sylvain Brun, Hsiao-Che Kuo, Chris E. Jeffree, Darren D. Thomson, Nick Read
Summary: This study used live-cell fluorescence imaging to observe the dynamic behavior of male and female nuclei during sexual reproduction in the model fungus Neurospora crassa for the first time. The specific movement of resident female nuclei within the trichogyne and the extraordinary migration of male nuclei to the protoperithecium were revealed, with male nuclei exhibiting dramatic speed and deformation not previously observed in a living organism. This research opens up new possibilities for studying cell signaling during sexual reproduction in fungi and nuclear dynamics in eukaryotes more broadly.
MICROBIOLOGY SPECTRUM
(2021)
Article
Microbiology
Zheng Wang, Francesc Lopez-Giraldez, Jason Slot, Oded Yarden, Frances Trail, Jeffrey P. Townsend
Summary: This study investigates the gene expression patterns of secondary metabolite clusters (SMCs) in the fungal model N. crassa and reveals their life cycle-specific expression. It also demonstrates the divergent activity of SMCs between asexual and sexual development. The findings highlight the importance of studying the function of SMCs in developmental switches and their ecological associations.
Article
Microbiology
Zhuo Zhu, Gengli Ma, Mingkun Yang, Can Tan, Guang Yang, Sen Wang, Na Li, Feng Ge, Shihua Wang
Summary: The Ras subfamily proteins in A. flavus were shown to have significant impact on growth, sporulation, sclerotia formation, and aflatoxin production. Rsr1 and Rheb were crucial for the pathogenicity of A. flavus.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Food Science & Technology
Vayu Maini Rekdal, Nabila Rodriguez-Valeron, Mikel Olaizola Garcia, Diego Prado Vasquez, Pia M. Sorensen, Rasmus Munk, Jay D. Keasling
Summary: This study explores novel culinary applications of Neurospora intermedia, an edible fungus traditionally used in Java, Indonesia. The fungus can be used to produce oncom-like meat alternatives and enzymes for starch-to-sugar conversion. It also adds orange color and distinct volatile aroma to dishes. The novel foods produced in this study received favorable ratings in consumer trials.
INTERNATIONAL JOURNAL OF GASTRONOMY AND FOOD SCIENCE
(2023)
Article
Microbiology
Antonio Franco-Cano, Ana T. Marcos, Joseph Strauss, David Canovas
Summary: Nitric oxide (NO) serves as a signaling molecule in both eukaryotic and prokaryotic organisms. In Aspergillus nidulans, NO levels transiently increase during conidiation induction. This study provides evidence for an additional biosynthetic pathway in A. nidulans involving l-arginine or an intermediate metabolite, apart from the existing nitrate reductase pathway.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Review
Engineering, Chemical
Huiru Ren, Hengli Qian, Qidong Hou, Weizun Li, Meiting Ju
Summary: In this review, common methods for the removal of ionic liquids (ILs) are systematically summarized and explained. The related factors, reaction pathways, and reaction mechanisms of each method are discussed. Furthermore, the future outlook on ILs degradation is presented.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Environmental Sciences
Beatriz Lima Santos Klienchen Dalari, Cristiane Lisboa Giroletti, Francisco J. Malaret, Everton Skoronski, Jason P. Hallett, William Gerson Matias, Rodrigo Costa Puerari, Maria Eliza Nagel-Hassemer
Summary: Textile dyeing processes generate aqueous effluents containing toxic dyes, which require new wastewater treatment processes. This study evaluated the use of an Ionic Liquid for Liquid-Liquid Extraction to extract and treat reactive dyes, showing high extraction yields for black, navy, and royal dyes. However, the extracted solution exhibited increased toxicity towards Daphnia magna due to traces of the Ionic Liquid. Further studies are needed to reduce the risk of toxicity transfer to water.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Review
Multidisciplinary Sciences
Neha Kaushik, Sarmistha Mitra, Eun Jung Baek, Linh Nhat Nguyen, Pradeep Bhartiya, June Hyun Kim, Eun Ha Choi, Nagendra Kumar Kaushik
Summary: This review critically examines methods for generating reactive oxygen species (ROS) and explores their potential as a solution for virus deactivation. The study highlights the ability of ROS to directly eradicate pathogens through oxidative stress and indirectly through non-oxidative mechanisms. Additionally, the novel and environmentally friendly cold plasma delivery system for virus destruction is discussed in detail.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Engineering, Biomedical
Ihn Han, In-Seok Song, Seung Ah Choi, Taebok Lee, Maksudbek Yusupov, Priyanka Shaw, Annemie Bogaerts, Eun Ha Choi, Jae Jun Ryu
Summary: This study proposes the use of low-dose nonthermal biocompatible dielectric barrier discharge plasma (DBD-NBP) on human gingival fibroblasts (HGFs) to enhance wound healing. The treatment inhibits colony formation but not cell death and induces cell migration through ECM degradation and MMP expression. The study also explores the effect of oxidation on the stability and conformation of FAK's catalytic kinase domain (KD).
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Wonsun Kim, JaeWoo Park, Yushika Aggarwal, Shital Sharma, Eun Ha Choi, Byoungchoo Park
Summary: In this study, a cathode-side passivation layer of poly(methyl methacrylate) (PMMA) was introduced to enhance the efficiency and stability of self-powered CH3NH3PbI3 perovskite-based photodiodes. The PMMA layer effectively suppressed interface recombination and reduced leakage/noise current in the perovskite film. The PMMA-passivated MAPbI(3) photodiodes demonstrated significantly high specific detectivity values and wide linear dynamic responses with rapid rise and decay response times.
Article
Plant Sciences
Azadeh Barjasteh, Pradeep Lamichhane, Zohreh Dehghani, Neha Kaushik, Ravi Gupta, Eun Ha Choi, Nagendra Kumar Kaushik
Summary: Seed germination is a complex process initiated by water uptake, and plasma treatment has emerged as an efficient method to promote germination. Plasma treatment increases the hydrophilicity and oxygen permeability of seed coats, while reactive oxygen- and nitrogen-enriched plasma-activated water induces hormonal changes in seeds to promote germination and plant growth. However, the molecular mechanism and germination behavior induced by plasma treatment still need further understanding and clarification. This review examines the effects of plasma treatment on sterilization, storage, seed shape variation, watering, and genetic and hormonal alterations to improve plant productivity.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Engineering, Chemical
Ju Sung Kim, Jinsung Choi, Young June Hong, Eun Ha Choi
Summary: An Ar-H-2 arc plasma jet under atmospheric pressure was used to remove tin (Sn) contamination on the multi-thin film surface of the Extreme ultraviolet (EUV) mirror. By converting Sn into SnH4, a gaseous compound that can be easily exhausted, the contamination was effectively removed. The discharge conditions, such as the content of H-2 in the mixture gas, applied power, and treatment time, were varied to investigate the plasma characteristics and the degree of Sn removal. The optimal case was found to be treating for at least 1 min under an applied power of 500 W using 5% of H-2 mixed content.
PLASMA CHEMISTRY AND PLASMA PROCESSING
(2023)
Review
Environmental Sciences
Paritosh Patel, Aditya Nandi, Suresh K. Verma, Neha Kaushik, Mrutyunjay Suar, Eun Ha Choi, Nagendra Kumar Kaushik
Summary: Emerging bio-contaminants, such as viruses, have had a significant impact on the health and environment of countries worldwide. Understanding the behavior of viruses is crucial in developing targeted therapeutic and vaccination treatments. Animal models, including non-human primates, rodents, and rabbits, offer advantages in assessing the risks of viral infections and chemical toxins, but their maintenance and experimental redundancy are issues. Therefore, exploring alternative animal models, such as zebrafish, is important. This review focuses on zebrafish traits and their potential in monitoring emerging bio-contaminants, particularly viruses like coronaviridae and poxviridae.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Physics, Multidisciplinary
Asad Ur Rehman, Amir Muhammad Afzal, Muhammad Waqas Iqbal, Saikh Mohammad Wabaidur, Essam A. Al-Ammar, Sohail Mumtaz, Eun Ha Choi, Muhammad Ali
Summary: In this research, polyaniline (PANI) was used to enhance the electrochemical capabilities of silver-zinc sulfide (AgZnS). The PANI was synthesized via polymerization of aniline, while the AgZnS was synthesized hydrothermally. Adding 50% PANI in AgZnS resulted in an excellent specific capacity (Q(s)) of 1121.6 Cg(-1). The assembled supercapattery device with 50% PANI exhibited a highest Q(s) of 223.44 Cg(-1), along with a tremendous energy density of 49.65 Wh kg(-1) at a power density of 795.33 W kg(-1).
Article
Physics, Applied
Prajwal Lamichhane, Tirtha R. Acharya, Jaewoo Park, Kirubel A. Amsalu, Byoungchoo Park, Eun Ha Choi
Summary: This study investigates the effects of nonthermal plasma characteristics on surface treatments of polyvinyl alcohol. Various diagnostics, such as Raman spectroscopy, work of adhesion, optical emission spectroscopy, and current-voltage measurements, were used. The findings highlight the importance of controlling the plasma's electron temperature and density to achieve desired surface treatment outcomes. Plasma jets with varying electron temperatures are found to be more suitable for different surface treatments, with high electron temperatures (>1.1 eV) suitable for functional group insertion and low electron temperatures for plasma etching, which increases surface roughness significantly.
PLASMA PROCESSES AND POLYMERS
(2023)
Article
Biochemistry & Molecular Biology
Davronjon Abduvokhidov, Maksudbek Yusupov, Aamir Shahzad, Pankaj Attri, Masaharu Shiratani, Maria C. Oliveira, Jamoliddin Razzokov
Summary: The potential of cold atmospheric plasma (CAP) in biomedical applications lies in its ability to generate reactive oxygen and nitrogen species (RONS). This study used molecular dynamics simulations to investigate the permeation capabilities of RONS across modified cell membranes, revealing that less hydrophilic species have a higher penetration ability through nitro-oxidized membranes. This research enhances our understanding of RONS translocation abilities in plasma biomedical applications and highlights the importance of further analyzing their role in intracellular signaling pathways.
Article
Energy & Fuels
Asad Ur Rehman, Amir Muhammad Afzal, Muhammad Waqas Iqbal, Muhammad Ali, Saikh Mohammad Wabaidur, Essam A. Al-Ammar, Sohail Mumtaz, Eun Ha Choi
Summary: Transition metal dichalcogenides (TMDs) are excellent candidates for energy storage applications due to their 2D structure and electrochemical features. However, their poor stability limits their potential as supercapattery electrode materials. In this study, tungsten disulfide (WS2) nanosheets were combined with hydrothermally synthesized silver zinc sulfide (AgZnS) nanostructures to create a hybrid electrode material with superior charge transfer and active redox sites. The AgZnS@WS2 hybrid electrode exhibited a high specific capacity and excellent electrochemical performance, making it suitable for industrial applications as a supercapattery electrode material.
JOURNAL OF ENERGY STORAGE
(2023)
Article
Chemistry, Physical
Muhammad Ali, Amir Muhammad Afzal, Muhammad Waqas Iqbal, Asad Ur Rehman, Saikh Mohammad Wabaidur, Essam A. Al-Ammar, Sohail Mumtaz, Eun Ha Choi
Summary: In this study, rGO and CoMnS nanostructures were synthesized and their structural and electrochemical properties were measured. The rGO@CoMnS composite electrode material showed a superior specific capacity of 1355.8 C/g compared to the reference electrode (747.6 C/g) in a 3-electrode measurement system. The estimated specific capacitance was 2238 F/g. This study provides new opportunities for designing high-performance energy devices.
Article
Chemistry, Physical
Min Ji Kim, Kien Tiek Wong, Minhee Kim, Choe Earn Choong, In Wook Nah, Yeomin Yoon, Eun Ha Choi, Min Jang
Summary: In this study, CeO2 with varying concentrations of oxygen vacancies (Ov) was prepared and the effects of Ov concentration and photoinduced electron transfer (PET) on O3 activation in visible-light-driven photocatalytic-ozonation (PO) for oxalic acid (OA) removal were investigated. The formation of Ov reduced the bandgap of CeO2 and created a region with low adsorption energy barrier for O3 around the Ce atom. Notably, there was no distinct linear correlation between Ov concentration and VLD-PO activity. CeO2 with a lower Ov amount exhibited better PET and higher photoexcited electron density, suggesting the crucial role of PET properties in Ov regeneration and O3 activation for OA removal. The electric energy per order (EEO) for VLD-PO using CeOx(7 0 0) was 6.94 kWh m 3 order 1. Bioassay experiments and continuous-flow experiments (CFEs) demonstrated the effectiveness of VLD-PO in treating toxic and refractory micropollutants in water. The findings of this study can promote the application of PO systems for water treatment.
APPLIED SURFACE SCIENCE
(2023)
Article
Engineering, Environmental
Shaik Abdul Munnaf, Min Jang, Eun Ha Choi
Summary: In this study, non-thermal plasma was used along with synthesized nano-particles to remove inorganic and organic compounds, achieving environmental safety. Plasma-generated reactive species were employed for oxidation and reduction processes. The maximum concentrations of H2O2 and NOx were found in deionized water. The removal capacity for arsenic was determined as 4.69 mg/g. The study also demonstrated the catalytic enhancement of synthesized nanoparticles in the removal of organic compounds. The results showed the potential of this eco-friendly method for pollutant removal and the recycling of waste material.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Review
Biochemistry & Molecular Biology
Desh Deepak Singh, Ihn Han, Eun-Ha Choi, Dharmendra Kumar Yadav
Summary: SARS-CoV-2 causes severe respiratory distress and organ failure, and supportive treatment options are currently used. Targeting viral proteins and enzymes, as well as developing antibodies and antiviral drugs, are effective approaches for COVID-19 treatment. Clinical studies are exploring various therapy categories. This article provides new insights into drug pathophysiology and development.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Engineering, Biomedical
Nagendra Kumar Kaushik, Pradeep Bhartiya, Neha Kaushik, Yungoh Shin, Linh Nhat Nguyen, Jang Sick Park, Doyoung Kim, Eun Ha Choi
Summary: This study demonstrates that nitric oxide enriched plasma-activated water (NO-PAW), generated by a microwave plasma system, effectively inhibits coronavirus infection in host lung cells and improves host antiviral responses.
BIOACTIVE MATERIALS
(2023)
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
