Article
Engineering, Environmental
You Zhang, Wenhong Fan, Xiaomin Li, Wen-Xiong Wang, Shu Liu
Summary: In this study, nanobubble technology and hydrogen water were combined for the first time to remove reactive oxygen species caused by environmental stress. Hydrogen nanobubbles showed remarkable capability in removing H2O2 and O(2)(center dot- ), and increasing the activity and expression of antioxidant enzymes.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Review
Chemistry, Applied
Xiaoyong Chen, Dongxiao Sun-Waterhouse, Wanzi Yao, Xiong Li, Mouming Zhao, Lijun You
Summary: Increasing studies focus on the degradation of polysaccharides by free radicals, particularly those generated from hydrogen peroxide (H2O2). Free radicals mainly break glycosidic bonds through hydrogen abstraction, affecting degradation efficiency. Various factors such as polysaccharide and H2O2 concentration, temperature, and pH impact degradation. Additionally, free radical degradation alters physicochemical and structural properties, enhancing bioactivities like antioxidant, antitumor, and anticoagulant activities.
Article
Chemistry, Organic
Rui Chen, Rongchen Wang, Jie Sun, Xuemei Dong, Chengjun Dong, Lixin Sun, Xianfeng Gu, Chunchang Zhao
Summary: An H2O2-activated, endoplasmic reticulum-targeted theranostic probe was developed, which can be activated by H2O2, leading to increased NIR fluorescence and photothermal signals for specific recognition of H2O2 and further photothermal therapy in H2O2-overexpressing cancer cells in the endoplasmic reticulum.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Cell Biology
Kevin Rychel, Justin Tan, Arjun Patel, Cameron Lamoureux, Ying Hefner, Richard Szubin, Josefin Johnsen, Elsayed Tharwat Tolba Mohamed, Patrick V. Phaneuf, Amitesh Anand, Connor A. Olson, Joon Ho Park, Anand V. Sastry, Laurence Yang, Adam M. Feist, Bernhard O. Palsson
Summary: The relationships between the genome, transcriptome, and metabolome play a crucial role in determining the characteristics of an organism. However, it has been challenging to study these relationships due to the complexity of the variables involved. A new data analytic method called iModulons has been developed to simplify the interpretation of the transcriptome by grouping genes into independently regulated sets. In this study, the researchers used iModulons along with resequencing and metabolic models to gain a deep understanding of the effects of causal mutations and metabolic rewiring in E. coli strains subjected to high levels of oxidative stress. The findings highlight the power of iModulon knowledge mapping in evolutionary analysis.
Review
Plant Sciences
Nadiyah M. Alabdallah, Md. Mahadi Hasan, Ines Hammami, Azzah Ibrahim Alghamdi, Dikhnah Alshehri, Hanan Ali Alatawi
Summary: Metal oxide nanoparticles are critical tools for overcoming crop productivity challenges, boosting crop production and resistance to abiotic stresses such as drought. They enhance osmotic adaptation and crop water balance by mitigating drought-induced reactive oxygen species. The impacts of different forms, features, and dosages of MONPs on plant growth and development under drought stress are summarized and discussed in this review.
Article
Biochemistry & Molecular Biology
Olivia Koufos, Ryan J. Mailloux
Summary: Dihydroorotate dehydrogenase (DHODH) is involved in pyrimidine biosynthesis and can be inhibited by S-glutathionylation, which is a negative feedback loop for hydrogen peroxide (H2O2) production. This redox modification can be influenced by sex differences in liver mitochondria.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Engineering, Environmental
Yanting Zhang, Na Zhang, Ao Qian, Chenglong Yu, Peng Zhang, Songhu Yuan
Summary: This study reveals the significant influence of the C/Fe molar ratio on the production of hydrogen peroxide and hydroxyl radical during the oxygenation of reduced iron and natural organic matter in the subsurface. The accumulation of hydroxyl radical increases more prominently with the increase in the C/Fe ratio.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Leanne E. Mocniak, Zachary T. Bitzer, Neil Trushin, John P. Richie
Summary: Tobacco smoke free radicals, which depend on the intrinsic nitrate levels in tobacco, are closely associated with adverse health effects. The levels of gas phase radicals are highly correlated with nitrate levels in different tobacco types and brands. Nitrate addition to tobacco leaves increases the production of gas phase radicals and NNK in smoke. Tobacco nitrate plays a key role in the production of gas phase radicals and has implications for health regulation.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Weiwei Zhu, Ti Fang, Wenjing Zhang, Ao Liang, Hui Zhang, Zhi-Ping Zhang, Xian-En Zhang, Feng Li
Summary: Dps, a natural protein nanocage, has been developed for antioxidant treatment by inhibiting the generation of ROS. With its ferroxidase activity, Dps not only protects cells from oxidative stress but also alleviates inflammation induced by ROS. This unique material shows great potential in various applications such as antiaging, anti-inflammation, and cosmetics.
Article
Plant Sciences
Chaofan Chen, Yi Zhang, Jianfa Cai, Yuting Qiu, Lihong Li, Chengxu Gao, Yiqun Gao, Meiyu Ke, Shengwei Wu, Chuan Wei, Jiaomei Chen, Tongda Xu, Jiri Friml, Junqi Wang, Ruixi Li, Daiyin Chao, Baocai Zhang, Xu Chen, Zhen Gao
Summary: Multi-copper oxidases SKU5 and SKS1 modulate ROS homeostasis in root apoplast to regulate cell wall formation. Loss of SKU5 and SKS1 function leads to aberrant cell wall structure and excessive ROS production, which can be rescued by decreasing ROS level or inhibiting NADPH oxidase activity.
Article
Chemistry, Analytical
Xia Zhang, Li Zhang, Xinyan Cheng, Shudi Liu, Shujing Fang, Liangwei Zhang, Xiaoyan Wang, Lingxin Chen
Summary: Mercury is a toxic heavy metal pollutant that induces oxidative stress and hypoxia stress. Excessive H2O2 can trigger oxidative stress. There is a need for powerful tools to study H2O2 fluctuations under mercury stress.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Mingxiu Wang, Feng Zhang, Cai-Qi Wang, Nan Yin, Yuting Wang, Guixin Qin, Qingling Xu, Jianhua Gong, Huizhen Liu, Xinrui Duan
Summary: Abnormal production of H2O2 is associated with cancer. It is crucial to develop fluorescent probes that can selectively image basal H2O2 in tumor cells. We have successfully developed a fluorescent probe, BBHP, which contains benzil as a recognition site for H2O2 and biotin as a target binding motif. BBHP enables a selective fluorescence turn-on response to H2O2 and can sufficiently image basal H2O2 in biotin receptor-positive cancer cells and tumor tissues.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Xiaoxiao Li, Xiaoyan Duan, Peng Yang, Lu Li, Bo Tang
Summary: In this study, robust surface-enhanced Raman spectroscopy (SERS) nanoprobes based on Au nanoparticles were developed for monitoring mitochondrial H2O2 in living cells, showing superior performance compared to traditional nanoprobes.
ANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Environmental
Yuting Wang, Huming Yan, Yongkang Yue, Yongbin Zhang, Fangjun Huo, Fangqin Cheng, Caixia Yin
Summary: Ferroptosis, a type of cell death caused by lipid peroxidation, is closely related to the development of tumors, ischemia-reperfusion injury, and other diseases. It involves the accumulation of reactive oxygen species and thiols, which promote or inhibit ferroptosis. Understanding the dynamic correlation between reactive oxygen species and active sulfur in these pathways is crucial for revealing the regulation mechanism of ferroptosis.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Yunfeng Yan, Xiaofei Huang, Yao Zhou, Jingyi Li, Feiyun Liu, Xueying Li, Xiaotao Hu, Jing Wang, Lingyan Guo, Renning Liu, Naoki Takaya, Shengmin Zhou
Summary: In Aspergillus nidulans, the peroxiredoxin (PrxA) plays a crucial role in protecting dormant conidia against high levels of H2O2, while catalase (CatB) becomes the main antioxidant during the mature hyphae stage. CatB needs to be secreted to function, and this process is limited to the mature hyphae stage.
Letter
Oncology
George Vartholomatos, George A. Alexou, Vissaria Tatsi, Haralambos Harissis, Georgios S. Markopoulos
Article
Biochemistry & Molecular Biology
Evrysthenis Vartholomatos, Stefania Mantziou, George A. Alexiou, Diamanto Lazari, Chrissa Sioka, Athanassios Kyritsis, Georgios S. Markopoulos
Summary: The study highlights the importance of natural substances as potential antiglioma agents and their targeting of CD markers on glioma cells. The identified NF-kappa B binding sites and core regulatory network provide potential targets for glioblastoma treatment.
Article
Surgery
K. Vaia Georvasili, S. Georgios Markopoulos, Anna Batistatou, Michael Mitsis, Thomas Messinis, D. Georgios Lianos, George Alexiou, George Vartholomatos, D. Christina Bali
Summary: The study introduces an iFC protocol for colorectal cancer detection, with a high accuracy in predicting the presence of cancer cells based on the tumor-index and good accuracy in tumor margin evaluation for rectal cancer patients.
INTERNATIONAL JOURNAL OF SURGERY
(2022)
Review
Oncology
Michail Mitsis, Panagiota Drosou, Vasileios Tatsis, Georgios S. Markopoulos
Summary: This study summarizes the role of neutrophil extracellular traps (NETs) in the development of pancreatic adenocarcinoma (PDAC). The results suggest that NETs have a dual role in PDAC progression and metastasis, promoting cancer cell proliferation and invasion while also potentially leading to cancer-associated thrombosis. The interplay between inflammation and cancer progression may be involved in these mechanisms.
Article
Biology
Zoi Anastasiadi, Stefania Mantziou, Christos Akrivis, Minas Paschopoulos, Eufemia Balasi, Georgios D. Lianos, George A. Alexiou, Michail Mitsis, George Vartholomatos, Georgios S. Markopoulos
Summary: In this study, the role of intraoperative flow cytometry in gynecological malignancies was investigated. The results confirmed its value in cancer cell identification and suggested the need for further validation in larger multicenter studies. Cell-cycle analysis showed a correlation between aneuploidy and poor prognosis in intraoperative tumor characterization.
Article
Oncology
Athanasios Paliouras, Georgios S. Markopoulos, Stavros Tsampalas, Stefania Mantziou, Ioannis Giannakis, Dimitrios Baltogiannis, Georgios K. Glantzounis, George A. Alexiou, Evangelia Lampri, Nikolaos Sofikitis, George Vartholomatos
Summary: Bladder cancer predominantly affects male patients, with surgical treatment being the primary option. Intraoperative flow cytometry (iFC) is highly specific, sensitive, and accurate in detecting cancer cells, based on cell proliferation and tumor aneuploidy. Further research on iFC as a novel malignancy evaluation tool during transurethral resections is supported.
Article
Oncology
George Vartholomatos, Georgios S. Markopoulos, Eyrysthenis Vartholomatos, Anna C. Goussia, Lefkothea Dova, Savvas Dimitriadis, Stefania Mantziou, Vaso Zoi, Anastasios Nasios, Chrissa Sioka, Athanasios P. Kyritsis, Spyridon Voulgaris, George A. Alexiou
Summary: Intraoperative Flow Cytometry (iFC) is a promising technique for glioma grading and resection margin assessment. It uses a fast cell cycle analysis protocol to evaluate the malignancy of tumor samples. The results showed that high-grade gliomas had a significantly higher tumor index and iFC could accurately identify malignant tissue in the resection margins. Comparative studies with other intraoperative adjuncts are needed.
Article
Microbiology
Angelos Liontos, Alexandros-George Asimakopoulos, Georgios S. S. Markopoulos, Dimitrios Biros, Lazaros Athanasiou, Stavros Tsourlos, Leukothea Dova, Iro-Chrisavgi Rapti, Ilias Tsiakas, Evangelia Ntzani, Evangelos Evangelou, Ioanna Tzoulaki, Konstantinos Tsilidis, George Vartholomatos, Evangelia Dounousi, Haralampos Milionis, Eirini Christaki
Summary: This study suggests that alterations in lymphocyte subpopulations are associated with COVID-19 severity markers. Increased levels of CD16CD56 cells are associated with higher risk of lung injury. An increase in CD3CD4 and CD4RO cell count results in a decrease in CRP levels. However, further evaluation in larger scale trials is needed to validate these findings.
Article
Biochemistry & Molecular Biology
Anila Duni, Athanasios Kitsos, Aris Bechlioulis, Georgios S. Markopoulos, Lampros Lakkas, Gerasimos Baxevanos, Michail Mitsis, George Vartholomatos, Katerina K. Naka, Evangelia Dounousi
Summary: This study investigated differences in circulating immune cells between type 2 cardiorenal syndrome (CRS-2) patients and CKD patients without cardiovascular disease (CVD). The results showed that CRS-2 patients had higher levels of proinflammatory cells and T regulatory cells, as well as lower levels of lymphocytes and natural killer cells compared to CKD patients. Decreased lymphocytes, T-lymphocytes, and increased proinflammatory cells were associated with mortality. CD4+ T-lymphocytes independently predicted fatal cardiovascular events in the CRS-2 cohort.
Article
Biochemistry & Molecular Biology
Maria Ntinopoulou, Dimitrios Cassimos, Eugenia Roupakia, Evangelos Kolettas, Maria Panopoulou, Elpis Mantadakis, Theocharis Konstantinidis, Akrivi Chrysanthopoulou
Summary: Elevated levels of neutrophil extracellular traps (NETs) and interleukin (IL)-17A were found in the sera of children during asthma exacerbation. The stimulation of control neutrophils with these sera resulted in IL-17A-enriched NET formation, which led to fibroblasts acquiring a pre-fibrotic phenotype and promoting fibrosis.
Article
Oncology
George A. Alexiou, Georgios S. Markopoulos, Evrysthenis Vartholomatos, Anna C. Goussia, Lefkothea Dova, Savvas Dimitriadis, Stefania Mantziou, Vasiliki Zoi, Anastasios Nasios, Chrissa Sioka, Athanasios P. Kyritsis, Spyridon Voulgaris, George Vartholomatos
Summary: This study investigated the role of intraoperative flow cytometry in differentiating low-grade (grade 1) from high-grade (grade 2-3) meningiomas. Intraoperative flow cytometry analysis using the 'Ioannina Protocol' was performed on 59 patients. The results showed that high-grade meningiomas had higher S-phase fraction, mitosis fraction, and tumor index, while low-grade meningiomas had lower G0/G1 phase fraction. ROC analysis identified a tumor index cutoff value of 11.4% for discriminating between low- and high-grade meningiomas.
Article
Medicine, Research & Experimental
Georgio S. S. Markopoulos
Summary: This study systematically catalogued the regulatory elements within HERVs and found that they are widely present in the human genome and participate in various functions such as inflammation, chemokine signaling, and response to viral infection.
Meeting Abstract
Transplantation
Anila Duni, Lampros Lakkas, Georgios Markopoulos, Aris Bechlioulis, Vasileios Koutlas, Eirini Tzalavra, Vasileios Tatsis, Ioanna Theodorou, Haralambos Pappas, George Vartholomatos, Michael Mitsis, Katerina Naka, Evangelia Ntounousi
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2022)
Meeting Abstract
Transplantation
Anila Duni, Lampros Lakkas, Aris Bechlioulis, Georgios Markopoulos, Revekka Georgiou, John Alekos, Athanasios Kitsos, Olga Balafa, Ioanna Theodorou, George Vartholomatos, Michael Mitsis, Katerina Naka, Evangelia Ntounousi
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2022)
Meeting Abstract
Transplantation
Anila Duni, Ioannis Mallioras, Georgios Markopoulos, Haralambos Pappas, Efthymios Pappas, Vasileios Koutlas, Eirini Tzalavra, Gerasimos Baxevanos, Silvia Priska, Georgios Katagis, Konstantina Gartzonika, George Vartholomatos, Mixalis Mitsis, Evangelia Ntounousi
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2022)
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
